##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.076990778916134	33.0	31.0	34.0	30.0	34.0
2	32.266373165855256	34.0	31.0	34.0	30.0	34.0
3	32.337439332497446	34.0	31.0	34.0	30.0	34.0
4	35.86283778950024	37.0	35.0	37.0	35.0	37.0
5	35.82160177267713	37.0	35.0	37.0	35.0	37.0
6	35.4180353330547	37.0	35.0	37.0	33.0	37.0
7	35.78588466302944	37.0	35.0	37.0	35.0	37.0
8	35.85354704861307	37.0	35.0	37.0	35.0	37.0
9	37.595338815529374	39.0	37.0	39.0	35.0	39.0
10	37.141991136614365	39.0	37.0	39.0	34.0	39.0
11	37.06696110878921	39.0	37.0	39.0	33.0	39.0
12	36.84882072541201	39.0	35.0	39.0	33.0	39.0
13	36.73306110615354	39.0	35.0	39.0	33.0	39.0
14	37.8429139980496	40.0	37.0	41.0	33.0	41.0
15	37.87438579448542	40.0	37.0	41.0	33.0	41.0
16	37.93043145937113	40.0	37.0	41.0	33.0	41.0
17	37.86241043424565	40.0	36.0	41.0	33.0	41.0
18	37.85212002093476	40.0	36.0	41.0	33.0	41.0
19	37.87649809666889	40.0	36.0	41.0	33.0	41.0
20	37.81101013980353	40.0	36.0	41.0	33.0	41.0
21	37.63352686690237	39.0	36.0	41.0	33.0	41.0
22	37.70954903666219	39.0	36.0	41.0	33.0	41.0
23	37.65107008249651	39.0	36.0	41.0	33.0	41.0
24	37.58862350190333	39.0	36.0	41.0	33.0	41.0
25	37.48425562998189	39.0	35.0	41.0	33.0	41.0
26	37.418480573220826	39.0	35.0	41.0	33.0	41.0
27	37.31326457996815	39.0	35.0	41.0	32.0	41.0
28	37.19863265144755	39.0	35.0	41.0	32.0	41.0
29	37.08954222156958	39.0	35.0	41.0	32.0	41.0
30	37.02538716126919	39.0	35.0	41.0	31.0	41.0
31	37.008719365029165	39.0	35.0	41.0	31.0	41.0
32	36.88300067397877	39.0	35.0	41.0	31.0	41.0
33	36.78072533670699	39.0	35.0	41.0	31.0	41.0
34	36.65543023566666	39.0	35.0	41.0	30.0	41.0
35	36.61261469876161	39.0	35.0	41.0	30.0	41.0
36	36.45076848640935	39.0	35.0	40.0	30.0	41.0
37	36.413599874993885	39.0	35.0	40.0	30.0	41.0
38	36.327263759144834	39.0	35.0	40.0	30.0	41.0
39	36.249497339854734	39.0	35.0	40.0	30.0	41.0
40	36.103451976188595	38.0	35.0	40.0	30.0	41.0
41	36.06545312835342	38.0	35.0	40.0	30.0	41.0
42	36.0273375579377	38.0	35.0	40.0	30.0	41.0
43	35.93560678798285	38.0	35.0	40.0	29.0	41.0
44	35.77402226012568	38.0	35.0	40.0	29.0	41.0
45	35.78194339331368	38.0	35.0	40.0	29.0	41.0
46	35.66236675740906	38.0	35.0	40.0	29.0	41.0
47	35.456950076622725	38.0	34.0	40.0	28.0	41.0
48	35.378420254003395	38.0	34.0	40.0	27.0	41.0
49	35.398802463976025	38.0	34.0	40.0	28.0	41.0
50	35.29196741557381	37.0	34.0	40.0	27.0	41.0
51	34.33107418661305	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	15.0
10	31.0
11	28.0
12	18.0
13	21.0
14	32.0
15	48.0
16	84.0
17	125.0
18	285.0
19	490.0
20	892.0
21	1340.0
22	2098.0
23	3230.0
24	4683.0
25	6820.0
26	9119.0
27	11241.0
28	13564.0
29	16160.0
30	20165.0
31	25517.0
32	32522.0
33	45010.0
34	74385.0
35	89949.0
36	94307.0
37	138156.0
38	222353.0
39	249568.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.68730020671192	4.760681057431275	51.61237184048918	16.93964689536762
2	21.77732720351523	5.20930994363429	50.8229883239767	22.190374528873775
3	22.487734715931126	5.338834355597225	49.64531396491545	22.5281169635562
4	19.946100524498565	5.399078268138124	48.64159390331605	26.01322730404726
5	18.13341767481089	6.377194666907643	49.65331510954979	25.836072548731675
6	21.371904498337642	7.3977641977958255	52.747498936318415	18.48283236754811
7	82.13165554036907	2.949880830010505	10.039083238260908	4.879380391359517
8	82.20150082647118	3.988994190227684	8.429064675605357	5.380440307695783
9	75.75634349572833	6.270449984374235	11.340069355804312	6.633137164093123
10	37.71636036402384	28.551661037626086	18.062254553121953	15.669724045228117
11	30.14671275325976	25.383019500201442	25.468772944458877	19.00149480207992
12	28.424960559063507	21.110125872124765	30.244044324458653	20.220869244353075
13	25.742882746519975	22.659618128899382	29.836927259240852	21.760571865339795
14	20.48405983726613	26.586391653205915	29.570536208474056	23.35901230105389
15	18.661493220677215	25.246058730284236	34.907017286237654	21.18543076280089
16	21.7099293263601	23.547462789970893	32.52550011860521	22.2171077650638
17	20.072330347494418	24.122886285849834	30.606072586384126	25.198710780271625
18	22.487169929251056	23.84115186360778	30.47202988098062	23.199648326160542
19	23.345928076298918	26.160165971979048	28.518621016841937	21.975284934880097
20	26.258627116538086	25.33614220575555	29.039260204753997	19.365970472952366
21	25.497482934029154	26.55598730359543	28.30033096499452	19.646198797380894
22	23.09902216599457	24.32555057288196	28.771080662833647	23.804346598289825
23	22.72946341500149	24.80994928215613	28.52737521038304	23.933212092459343
24	23.81780734749818	23.97943046911182	28.66339466916679	23.539367514223212
25	21.79238818165046	25.909118292687516	26.521347053884416	25.777146471777613
26	20.18058112784135	27.13225797949448	27.8917078019632	24.795453090700974
27	21.43704322877249	26.556646221388846	28.36546969542937	23.64084085440929
28	20.821237485268483	26.6916302379258	29.425386031695826	23.061746245109887
29	22.718450074740105	25.170942101834804	28.447552026266347	23.663055797158748
30	23.274859085723325	25.690075191933342	28.173724617545286	22.861341104798054
31	23.710686140511395	26.802704951673086	24.990116233098757	24.49649267471676
32	23.567512717113413	27.357513733729437	25.36692307981942	23.70805046933773
33	22.904076630256753	26.43945298527413	25.60083889648213	25.055631487986986
34	21.912028826712152	24.651432487282886	27.609690986381107	25.82684769962385
35	20.78179654877686	24.756953465342807	26.9248871367951	27.536362849085233
36	21.604879003866905	27.99101612654234	25.507837356497117	24.896267513093637
37	22.021503311532566	27.591241288165456	27.001886387511437	23.385369012790534
38	23.453425807739084	27.109195856724916	25.601968469842273	23.835409865693727
39	22.95933159379036	25.043488574365462	26.54732724116768	25.449852590676503
40	21.733932760263116	24.07243200909683	29.544367758964107	24.649267471675948
41	18.41543449039298	25.12142913621525	28.64823955991822	27.81489681347355
42	21.008652531938687	24.707346368609908	27.143365450869204	27.140635648582194
43	22.102079544556023	24.591376836968674	26.911143994246707	26.395399624228595
44	22.370541479816406	24.136347035058193	26.888834920383903	26.604276564741497
45	22.19555174010776	24.220782643728796	26.006543994999753	27.577121621163688
46	21.4339369020321	25.497200540689114	27.446938291407335	25.62192426587145
47	19.87832612289005	25.71332557692959	29.55396913252531	24.854379167655043
48	20.445842605248	23.832303538953337	29.5366490076698	26.185204848128862
49	21.52242014857655	22.86256480927154	29.579949319808573	26.035065722343337
50	20.892306475844073	22.951612842496054	28.149250528075548	28.00683015358432
51	19.335942647795264	23.47234616152146	26.96084522209295	30.230865968590333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	1206.5
2	2115.0
3	3814.5
4	5514.0
5	3944.5
6	2375.0
7	2345.0
8	2315.0
9	2497.0
10	2679.0
11	2573.0
12	2467.0
13	2518.5
14	2570.0
15	2436.5
16	2303.0
17	2453.5
18	2604.0
19	2662.0
20	2720.0
21	2996.0
22	3272.0
23	4042.5
24	4813.0
25	5437.0
26	8250.0
27	10439.0
28	11162.5
29	11886.0
30	12825.5
31	13765.0
32	16985.0
33	20205.0
34	24216.5
35	28228.0
36	28757.0
37	29286.0
38	33796.0
39	38306.0
40	39658.0
41	41010.0
42	48007.0
43	55004.0
44	58366.0
45	61728.0
46	66728.5
47	71729.0
48	82698.5
49	93668.0
50	103044.5
51	112421.0
52	107421.0
53	102421.0
54	88481.0
55	74541.0
56	67406.5
57	60272.0
58	55060.0
59	49848.0
60	47248.0
61	44648.0
62	38015.0
63	31382.0
64	26666.5
65	21951.0
66	18146.0
67	14341.0
68	12792.5
69	11244.0
70	9325.0
71	7406.0
72	6786.5
73	6167.0
74	5026.0
75	3032.0
76	2179.0
77	1789.5
78	1400.0
79	998.5
80	597.0
81	388.0
82	179.0
83	123.5
84	68.0
85	45.5
86	23.0
87	14.0
88	5.0
89	6.0
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1062348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.847797693670746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92586572869828	19.042032943325463
2	11.548816234691586	6.201205637407599
3	4.997859634120594	4.0254457307469895
4	2.6684544981414415	2.8656850608346844
5	1.5743136168366707	2.1133426745611006
6	1.0090370876752652	1.625425415718974
7	0.725305617655593	1.3631000942230185
8	0.5441265176533816	1.1686878932575642
9	0.3941986893016216	0.9525029995332095
>10	4.114260326950125	27.06996620610479
>50	1.3496862532773468	24.526515348320814
>100	0.1395738354767426	6.062270774364122
>500	0.006376469641068667	1.2294594421204368
>1k	0.002125489880356222	1.7543597794812082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	4219	0.397139167203214	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	3818	0.35939259075180635	TruSeq Adapter, Index 8 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3734	0.35148557723081325	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2830	0.26639105076679204	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2718	0.25584836607213457	No Hit
TTTTTGGGGAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGATTG	1127	0.10608576473999105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.41311133451562E-5	0.0	0.0	0.39337392266940774	0.0
2	9.41311133451562E-5	0.0	0.0	1.5369728186997105	0.0
3	9.41311133451562E-5	0.0	0.0	2.1059953988711797	0.0
4	9.41311133451562E-5	0.0	0.0	2.8315580205356436	0.0
5	9.41311133451562E-5	0.0	0.0	4.051026593922142	0.0
6	9.41311133451562E-5	0.0	0.0	4.7716943976926585	0.0
7	9.41311133451562E-5	0.0	0.0	5.438989860196471	0.0
8	9.41311133451562E-5	0.0	0.0	6.524603990406157	0.0
9	9.41311133451562E-5	0.0	0.0	7.036206591437081	0.0
10	9.41311133451562E-5	0.0	0.0	8.142247173242666	0.0
11	9.41311133451562E-5	0.0	0.0	9.952200220643329	0.0
12	9.41311133451562E-5	0.0	0.0	11.69748519317587	0.0
13	1.882622266903124E-4	0.0	0.0	12.251823319665496	0.0
14	1.882622266903124E-4	0.0	0.0	12.449875182143705	0.0
15	1.882622266903124E-4	0.0	0.0	12.774062736504423	0.0
16	1.882622266903124E-4	0.0	0.0	13.506873453896464	0.0
17	1.882622266903124E-4	0.0	0.0	14.535067604965604	0.0
18	1.882622266903124E-4	0.0	0.0	15.670006438568153	0.0
19	1.882622266903124E-4	0.0	0.0	16.395569060232617	0.0
20	1.882622266903124E-4	0.0	0.0	17.198789850406836	0.0
21	1.882622266903124E-4	0.0	0.0	18.21540587453452	0.0
22	1.882622266903124E-4	0.0	0.0	19.242941107810246	0.0
23	1.882622266903124E-4	0.0	0.0	20.202984332817493	0.0
24	1.882622266903124E-4	0.0	0.0	20.957821730732302	0.0
25	1.882622266903124E-4	0.0	0.0	21.61824562196192	0.0
26	1.882622266903124E-4	0.0	0.0	22.205529638122346	0.0
27	2.823933400354686E-4	0.0	0.0	22.77878811839435	0.0
28	3.765244533806248E-4	0.0	0.0	23.378968097083064	0.0
29	3.765244533806248E-4	0.0	0.0	24.029790614751477	0.0
30	3.765244533806248E-4	0.0	0.0	24.737750718220394	0.0
31	3.765244533806248E-4	0.0	0.0	25.396009593843072	0.0
32	3.765244533806248E-4	0.0	0.0	26.085143474642962	0.0
33	3.765244533806248E-4	0.0	0.0	26.71582193405551	0.0
34	3.765244533806248E-4	0.0	0.0	27.350924555795277	0.0
35	3.765244533806248E-4	0.0	0.0	28.02302070507969	0.0
36	3.765244533806248E-4	0.0	0.0	28.65793506459277	0.0
37	3.765244533806248E-4	0.0	0.0	29.324948133756546	0.0
38	4.70655566725781E-4	0.0	0.0	29.977935667031897	0.0
39	4.70655566725781E-4	0.0	0.0	30.633747133707597	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.033043E-4	45.000004	24
CCGGCAT	20	7.033043E-4	45.000004	17
ATTCGCA	20	7.033043E-4	45.000004	39
TCTAGCG	20	7.033043E-4	45.000004	1
CTCGCTA	20	7.033043E-4	45.000004	22
CGTAATG	20	7.033043E-4	45.000004	33
CGTCGTG	20	7.033043E-4	45.000004	18
TCGAGGT	20	7.033043E-4	45.000004	38
CGCAACA	20	7.033043E-4	45.000004	22
TTCGATA	20	7.033043E-4	45.000004	31
TCGAAGA	20	7.033043E-4	45.000004	38
TACACGA	20	7.033043E-4	45.000004	17
TCGAAAG	20	7.033043E-4	45.000004	26
TAACTCG	20	7.033043E-4	45.000004	29
CTAGCGA	20	7.033043E-4	45.000004	27
CGCATCG	25	3.8906066E-5	45.0	21
TCGCTAG	25	3.8906066E-5	45.0	15
ACTACGT	35	1.2118107E-7	45.0	14
TAGTCGG	25	3.8906066E-5	45.0	33
ACGGTTG	25	3.8906066E-5	45.0	12
>>END_MODULE