FastQCFastQC Report
Sat 14 Jan 2017
SRR2934325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460077
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG294322.0157840990577895No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC285821.9575679912771724No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC256661.757852496820373No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT84420.5781886845693754No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC61410.4205942563303168No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT60760.41614243632356374No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT59620.40833462892710454No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC58500.4006638006077761No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.3960750015238922No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA51060.3497075839150949No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT33780.23135766127402868No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG21530.1474579765313747No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC21320.14601969622150066No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC20570.14088298082909326No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT20260.13875980513356487No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC19630.13444496420394267No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT17440.11944575525811309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT253.8913262E-545.032
AGTCGAC253.8913262E-545.028
CCGATGA31600.043.71835718
CGATGAA32250.043.4651219
CACGACC1350.043.33333627
ACCGATG32400.042.517
GATGAAT34150.041.96925420
ACCACCG41250.041.89090714
CCACCGA40250.041.53416415
TACGGCT35050.041.2125557
CGACCAC58450.041.03507212
CGGGTAT1700.041.029416
CGTTTTT28400.040.9595071
TGAATGA34400.040.7485522
ACGGCTG35700.040.5882388
TAGCCGT501.0822987E-940.544
GCGATAG501.0822987E-940.59
ATACCGG501.0822987E-940.528
GACCACC58100.040.35283713
CCCCGTA1400.040.1785740
Produced by FastQC (version 0.11.5)