##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1460077
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98392276571715	33.0	31.0	34.0	30.0	34.0
2	32.17548663529389	34.0	31.0	34.0	30.0	34.0
3	32.256687147321685	34.0	31.0	34.0	30.0	34.0
4	35.84719641498359	37.0	35.0	37.0	35.0	37.0
5	35.74746537340154	37.0	35.0	37.0	35.0	37.0
6	35.067516302222415	37.0	35.0	37.0	32.0	37.0
7	35.62804427437731	37.0	35.0	37.0	35.0	37.0
8	35.54623694503783	37.0	35.0	37.0	33.0	37.0
9	37.45597184258091	39.0	37.0	39.0	35.0	39.0
10	37.05596006238027	39.0	37.0	39.0	33.0	39.0
11	36.940456564961984	39.0	37.0	39.0	33.0	39.0
12	36.639205329581934	39.0	35.0	39.0	33.0	39.0
13	36.38694397624235	39.0	35.0	39.0	32.0	39.0
14	37.14275959418578	40.0	36.0	41.0	31.0	41.0
15	37.21340586832064	40.0	36.0	41.0	31.0	41.0
16	37.542089903477695	40.0	36.0	41.0	32.0	41.0
17	37.352482780017766	39.0	35.0	41.0	32.0	41.0
18	37.45254531096648	39.0	36.0	41.0	32.0	41.0
19	37.46343103822607	39.0	36.0	41.0	32.0	41.0
20	37.06616842810345	39.0	35.0	41.0	31.0	41.0
21	37.12425646044695	39.0	35.0	41.0	31.0	41.0
22	37.34096968858492	39.0	35.0	41.0	32.0	41.0
23	37.14266644841334	39.0	35.0	41.0	32.0	41.0
24	37.167804848648395	39.0	35.0	41.0	32.0	41.0
25	37.169592425604954	39.0	35.0	41.0	32.0	41.0
26	37.04616400367926	39.0	35.0	41.0	32.0	41.0
27	36.81420226467508	39.0	35.0	41.0	31.0	41.0
28	36.72307145445069	39.0	35.0	41.0	31.0	41.0
29	36.65775229662545	39.0	35.0	41.0	31.0	41.0
30	36.60722482444419	39.0	35.0	40.0	30.0	41.0
31	36.507558163028385	39.0	35.0	40.0	30.0	41.0
32	36.25988423898192	39.0	35.0	40.0	30.0	41.0
33	36.163000992413416	38.0	35.0	40.0	30.0	41.0
34	35.884152685098115	38.0	35.0	40.0	29.0	41.0
35	35.766373965208686	38.0	35.0	40.0	29.0	41.0
36	35.58138714602038	38.0	34.0	40.0	27.0	41.0
37	35.45265831870511	38.0	34.0	40.0	27.0	41.0
38	35.38148330533253	38.0	34.0	40.0	26.0	41.0
39	35.28134132651908	38.0	34.0	40.0	26.0	41.0
40	35.17534828642599	38.0	34.0	40.0	25.0	41.0
41	34.902411311184274	38.0	34.0	40.0	23.0	41.0
42	35.01970854961759	38.0	34.0	40.0	24.0	41.0
43	34.91878373537834	38.0	34.0	40.0	24.0	41.0
44	34.87372309816537	38.0	34.0	40.0	24.0	41.0
45	35.00660033683155	38.0	34.0	40.0	26.0	41.0
46	34.8787200948991	38.0	34.0	40.0	25.0	41.0
47	34.47763713831531	37.0	33.0	40.0	23.0	41.0
48	34.50127082338808	37.0	33.0	40.0	23.0	41.0
49	34.55665146427209	37.0	33.0	40.0	24.0	41.0
50	34.344001035561824	37.0	33.0	40.0	24.0	41.0
51	33.44874414157609	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	14.0
10	24.0
11	23.0
12	24.0
13	24.0
14	41.0
15	62.0
16	110.0
17	251.0
18	534.0
19	1346.0
20	3141.0
21	6103.0
22	8888.0
23	11168.0
24	13191.0
25	15105.0
26	17122.0
27	19496.0
28	21576.0
29	25693.0
30	31689.0
31	40933.0
32	54307.0
33	74864.0
34	113795.0
35	112099.0
36	118278.0
37	168370.0
38	265844.0
39	335820.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.76746568845342	3.680764781583437	47.502152283749425	15.049617246213728
2	18.43957544704834	16.768088258358976	45.95435720170922	18.837979092883458
3	18.64607140582312	17.01656830427436	44.8688665049857	19.468493784916824
4	16.988624572539667	4.247926650443778	56.98274817013075	21.7807006068858
5	26.880637117083552	4.990969654340148	45.001051314416976	23.12734191415932
6	19.424112563926425	15.661708252372991	48.0728071190766	16.84137206462399
7	75.13596885643702	1.928322958309733	18.748942692748397	4.186765492504847
8	75.66717371755051	11.587539561269713	8.537494940335339	4.207791780844436
9	71.76470829963077	4.237516240581832	8.922680105227327	15.075095354560068
10	43.25710219392539	26.359911155370575	16.36612315651846	14.01686349418558
11	34.07690142369204	20.6529518648674	27.11226873651184	18.15787797492872
12	26.998096675723264	18.853183770444986	29.55440021313944	24.594319340692305
13	28.846560832065705	18.95982198199136	34.8611751298048	17.332442056138138
14	20.331461970841264	27.037067223167	31.985367894980882	20.646102911010857
15	16.914792849966133	21.45804639070405	39.464288527248904	22.16287223208091
16	22.981596176092083	22.867287136226377	27.504782282030334	26.646334405651206
17	23.77778706191523	24.65048076231596	30.70276430626604	20.86896786950277
18	23.872713562366915	21.358325622552783	30.18039459562749	24.588566219452808
19	23.108849738746656	24.61781125242025	28.11358579033845	24.159753218494643
20	23.903191407028533	27.990989516306332	31.766406840187194	16.33941223647794
21	22.137188655118877	30.039374635721266	31.012405510120356	16.8110311990395
22	19.606089267894774	20.16195036289182	36.667723688545195	23.56423668066821
23	21.615229881711716	26.115061054999156	32.26391484832649	20.005794214962634
24	21.40681621585711	27.1737723421436	27.497659370019527	23.921752071979764
25	22.44552855774045	31.339237588154596	24.860606666634705	21.35462718747025
26	20.10846003327222	23.121177855688433	30.095467567806356	26.674894543232995
27	22.076575413488467	25.300309504224778	29.35564357222256	23.267471510064194
28	17.249843672628224	27.249864219489794	31.012884936890316	24.487407170991666
29	23.43218885031406	24.586100596064455	29.26749753608885	22.714213017532636
30	22.35286221206142	29.872671098853004	28.853478275460816	18.920988413624762
31	23.33691990216954	25.580431716957392	26.680305216779665	24.402343164093402
32	27.64724052224643	25.593924156054783	26.338405440261027	20.42042988143776
33	24.99580501576287	23.76532196589632	24.29604740023985	26.94282561810096
34	23.60971373427566	25.781448512646936	28.170843044579158	22.437994708498252
35	21.643995487909198	27.755111545486983	25.892333075584368	24.708559891019448
36	26.75228772181193	28.805261640310754	23.41958677521802	21.022863862659296
37	19.956892684426915	30.08259153455605	29.217157725243258	20.74335805577377
38	22.46949989623835	27.052340390267087	24.36323563757254	26.114924075922026
39	21.820972455562277	25.941987991044307	27.74381077162369	24.49322878176973
40	21.42626724480969	22.383819483492996	27.422937283444643	28.766975988252675
41	22.379025215793412	26.874267589996965	24.551787337243173	26.194919856966447
42	20.36988460197647	22.75297809636067	31.115071328429938	25.762065973232918
43	24.643494829382288	22.249511498366182	27.403417764953492	25.70357590729804
44	22.594630283197393	23.280963949161585	29.35461622914408	24.76978953849694
45	20.0205194657542	22.558399317296278	27.42478650098591	29.99629471596361
46	24.586100596064455	24.375974691745707	27.790657616002445	23.247267096187393
47	17.11423438626867	25.261681404473872	34.636050016540224	22.988034192717233
48	20.286053406772382	29.02853753603406	27.127678882689064	23.557730174504496
49	21.802274811533913	22.23047140664499	32.041734785220235	23.925518996600864
50	21.949732788065287	23.047346133114896	28.014070490802883	26.988850588016934
51	20.50439805571898	24.373509068357354	26.016025182233538	29.10606769369013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	974.5
2	1738.0
3	5589.0
4	9440.0
5	6221.5
6	3003.0
7	2917.0
8	2831.0
9	2949.5
10	3068.0
11	3069.5
12	3071.0
13	3023.5
14	2976.0
15	3002.5
16	3029.0
17	2988.0
18	2947.0
19	3433.0
20	3919.0
21	4118.5
22	4318.0
23	5115.5
24	5913.0
25	7228.5
26	10903.5
27	13263.0
28	15224.5
29	17186.0
30	20532.5
31	23879.0
32	27425.0
33	30971.0
34	34422.0
35	37873.0
36	40210.0
37	42547.0
38	47201.0
39	51855.0
40	55713.0
41	59571.0
42	64504.5
43	69438.0
44	76632.5
45	83827.0
46	148734.5
47	213642.0
48	179242.5
49	144843.0
50	138825.5
51	132808.0
52	117424.5
53	102041.0
54	96067.0
55	90093.0
56	82757.0
57	75421.0
58	69848.0
59	64275.0
60	53853.0
61	43431.0
62	37950.0
63	32469.0
64	28803.5
65	25138.0
66	21023.0
67	16908.0
68	14400.0
69	11892.0
70	9836.0
71	7780.0
72	6543.0
73	5306.0
74	4676.5
75	3119.0
76	2191.0
77	1692.5
78	1194.0
79	850.0
80	506.0
81	429.5
82	353.0
83	256.5
84	160.0
85	122.0
86	84.0
87	75.0
88	66.0
89	36.5
90	7.0
91	5.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1460077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.207221822245273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63631502813126	19.560104639372145
2	10.158911463549597	5.324736923955894
3	3.7792975022251984	2.9713466391921983
4	1.9658251436002134	2.0607526240831184
5	1.14087895619965	1.494963393872794
6	0.7799740349211985	1.2264571529262918
7	0.5748555512070689	1.0545756862362912
8	0.44491306395749824	0.9327948286999165
9	0.3643679781647695	0.8594165185818424
>10	5.0635239402375705	32.865905815811296
>50	0.9926158561121884	16.721268134061607
>100	0.09035526263167469	4.240444088326801
>500	0.002634264213577838	0.49195585615499654
>1k	0.002897690634935622	1.424849412739682
>5k	0.0018439849495044864	2.993433331866496
>10k+	7.902792640733514E-4	5.776994954118736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	29432	2.0157840990577895	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	28582	1.9575679912771724	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC	25666	1.757852496820373	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAATCACGT	8442	0.5781886845693754	No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	6141	0.4205942563303168	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCT	6076	0.41614243632356374	No Hit
GAACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT	5962	0.40833462892710454	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTC	5850	0.4006638006077761	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5783	0.3960750015238922	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAATCACGTCGTA	5106	0.3497075839150949	No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGCT	3378	0.23135766127402868	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCG	2153	0.1474579765313747	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	2132	0.14601969622150066	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGC	2057	0.14088298082909326	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAATCACGTCGT	2026	0.13875980513356487	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC	1963	0.13444496420394267	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	1744	0.11944575525811309	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.739581542617273E-4	0.0	0.0	0.25320582407640146	0.0
2	2.739581542617273E-4	0.0	0.0	1.03809593603625	0.0
3	2.739581542617273E-4	0.0	0.0	1.3405457383411972	0.0
4	2.739581542617273E-4	0.0	0.0	2.1350928752387714	0.0
5	2.739581542617273E-4	0.0	0.0	4.61297589099753	0.0
6	2.739581542617273E-4	0.0	0.0	5.212533311599319	0.0
7	2.739581542617273E-4	0.0	0.0	6.104061635105546	0.0
8	2.739581542617273E-4	0.0	0.0	7.132363567126939	0.0
9	2.739581542617273E-4	0.0	0.0	7.469195117791733	0.0
10	2.739581542617273E-4	0.0	0.0	10.3292497587456	0.0
11	2.739581542617273E-4	0.0	0.0	11.403097233913005	0.0
12	2.739581542617273E-4	0.0	0.0	14.646693290833293	0.0
13	2.739581542617273E-4	0.0	0.0	15.123380479248697	0.0
14	2.739581542617273E-4	0.0	0.0	15.385147495645777	0.0
15	2.739581542617273E-4	0.0	0.0	15.988814288561494	0.0
16	2.739581542617273E-4	0.0	0.0	16.519676701982156	0.0
17	2.739581542617273E-4	0.0	0.0	17.092865650236256	0.0
18	3.424476928271591E-4	0.0	0.0	17.669616054495755	0.0
19	3.424476928271591E-4	0.0	0.0	18.85023872028667	0.0
20	3.424476928271591E-4	0.0	0.0	19.423427668540768	0.0
21	3.424476928271591E-4	0.0	0.0	20.049696009183076	0.0
22	3.424476928271591E-4	0.0	0.0	20.8120530629549	0.0
23	3.424476928271591E-4	0.0	0.0	21.42626724480969	0.0
24	3.424476928271591E-4	0.0	0.0	21.905077608920624	0.0
25	4.1093723139259096E-4	0.0	0.0	22.348410392054667	0.0
26	4.1093723139259096E-4	0.0	0.0	22.743732008654337	0.0
27	4.1093723139259096E-4	0.0	0.0	23.28582670639973	0.0
28	4.1093723139259096E-4	0.0	0.0	23.72210506706153	0.0
29	4.1093723139259096E-4	0.0	0.0	24.236256033072227	0.0
30	4.1093723139259096E-4	0.0	0.0	24.801226236698476	0.0
31	4.7942676995802277E-4	0.0	0.0	25.22154653487453	0.0
32	4.7942676995802277E-4	0.0	0.0	25.649674640447046	0.0
33	4.7942676995802277E-4	0.0	0.0	26.093007423581085	0.0
34	4.7942676995802277E-4	0.0	0.0	26.605857088359038	0.0
35	4.7942676995802277E-4	0.0	0.0	27.149253087337176	0.0
36	4.7942676995802277E-4	0.0	0.0	27.589846288928598	0.0
37	4.7942676995802277E-4	0.0	0.0	28.07214961950637	0.0
38	4.7942676995802277E-4	0.0	0.0	28.708965349087755	0.0
39	4.7942676995802277E-4	0.0	0.0	30.090878768722472	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAGT	25	3.8913262E-5	45.0	32
AGTCGAC	25	3.8913262E-5	45.0	28
CCGATGA	3160	0.0	43.718357	18
CGATGAA	3225	0.0	43.46512	19
CACGACC	135	0.0	43.333336	27
ACCGATG	3240	0.0	42.5	17
GATGAAT	3415	0.0	41.969254	20
ACCACCG	4125	0.0	41.890907	14
CCACCGA	4025	0.0	41.534164	15
TACGGCT	3505	0.0	41.212555	7
CGACCAC	5845	0.0	41.035072	12
CGGGTAT	170	0.0	41.02941	6
CGTTTTT	2840	0.0	40.959507	1
TGAATGA	3440	0.0	40.74855	22
ACGGCTG	3570	0.0	40.588238	8
TAGCCGT	50	1.0822987E-9	40.5	44
GCGATAG	50	1.0822987E-9	40.5	9
ATACCGG	50	1.0822987E-9	40.5	28
GACCACC	5810	0.0	40.352837	13
CCCCGTA	140	0.0	40.17857	40
>>END_MODULE