##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934324.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651082
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08812100472751	33.0	31.0	34.0	30.0	34.0
2	32.280133685157935	34.0	31.0	34.0	30.0	34.0
3	32.34139939362477	34.0	31.0	34.0	30.0	34.0
4	35.874693203006686	37.0	35.0	37.0	35.0	37.0
5	35.83148512783336	37.0	35.0	37.0	35.0	37.0
6	35.466173231635956	37.0	35.0	37.0	33.0	37.0
7	35.818941392942826	37.0	35.0	37.0	35.0	37.0
8	35.89070193923346	37.0	35.0	37.0	35.0	37.0
9	37.63378191994249	39.0	37.0	39.0	35.0	39.0
10	37.14683250343275	39.0	37.0	39.0	34.0	39.0
11	37.07432397148132	39.0	37.0	39.0	33.0	39.0
12	36.869105581171034	39.0	35.0	39.0	33.0	39.0
13	36.75805198116366	39.0	35.0	39.0	33.0	39.0
14	37.88956383374137	40.0	37.0	41.0	33.0	41.0
15	37.941815316657504	40.0	37.0	41.0	33.0	41.0
16	37.97401095407337	40.0	37.0	41.0	33.0	41.0
17	37.91021561032251	40.0	36.0	41.0	33.0	41.0
18	37.88221606495034	39.0	36.0	41.0	33.0	41.0
19	37.9008174085599	40.0	37.0	41.0	33.0	41.0
20	37.84687643031139	40.0	36.0	41.0	33.0	41.0
21	37.69028171566715	39.0	36.0	41.0	33.0	41.0
22	37.75869398938997	39.0	36.0	41.0	33.0	41.0
23	37.700770409871566	39.0	36.0	41.0	33.0	41.0
24	37.641321676839475	39.0	36.0	41.0	33.0	41.0
25	37.566017798065374	39.0	36.0	41.0	33.0	41.0
26	37.504828884840926	39.0	36.0	41.0	33.0	41.0
27	37.42699076306825	39.0	35.0	41.0	33.0	41.0
28	37.34439287217278	39.0	35.0	41.0	32.0	41.0
29	37.24286341812552	39.0	35.0	41.0	32.0	41.0
30	37.18491833593925	39.0	35.0	41.0	32.0	41.0
31	37.12226570539502	39.0	35.0	41.0	32.0	41.0
32	36.98567615139107	39.0	35.0	41.0	31.0	41.0
33	36.89783468134582	39.0	35.0	41.0	31.0	41.0
34	36.72083700670576	39.0	35.0	41.0	31.0	41.0
35	36.661896658178236	39.0	35.0	41.0	31.0	41.0
36	36.468725905492704	39.0	35.0	40.0	30.0	41.0
37	36.40328407174519	39.0	35.0	40.0	30.0	41.0
38	36.35306459094246	39.0	35.0	40.0	30.0	41.0
39	36.278293671150486	39.0	35.0	40.0	30.0	41.0
40	36.1280637461948	38.0	35.0	40.0	30.0	41.0
41	36.09321099339254	38.0	35.0	40.0	30.0	41.0
42	36.0403497562519	38.0	35.0	40.0	30.0	41.0
43	35.94166479798243	38.0	35.0	40.0	30.0	41.0
44	35.755135297858025	38.0	35.0	40.0	29.0	41.0
45	35.83814788306235	38.0	35.0	40.0	29.0	41.0
46	35.7505644450315	38.0	35.0	40.0	29.0	41.0
47	35.56768886253959	38.0	34.0	40.0	28.0	41.0
48	35.47004985547135	38.0	34.0	40.0	28.0	41.0
49	35.47349642594942	38.0	34.0	40.0	28.0	41.0
50	35.3204788336953	37.0	34.0	40.0	27.0	41.0
51	34.3681594637849	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	25.0
11	12.0
12	7.0
13	12.0
14	17.0
15	24.0
16	35.0
17	72.0
18	119.0
19	237.0
20	437.0
21	821.0
22	1152.0
23	1814.0
24	2670.0
25	3825.0
26	5368.0
27	6535.0
28	7742.0
29	9480.0
30	11901.0
31	15249.0
32	19945.0
33	27388.0
34	45139.0
35	56310.0
36	58680.0
37	85974.0
38	139397.0
39	150640.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.53070427380883	4.698025747908866	52.42565452585082	18.345615452431492
2	21.241256861654907	4.877112253141693	50.71987860208085	23.161752283122556
3	21.42664057676299	5.085380950479356	51.1424060256619	22.34557244709576
4	20.677426192092547	5.274911608676019	49.29425172251729	24.753410476714148
5	18.540061006140547	6.433291044753195	49.1956466312999	25.831001317806358
6	21.686976448435065	6.772879606562614	53.51077130069638	18.02937264430594
7	81.64148294684847	2.7151725896277275	10.528166959000556	5.11517750452324
8	81.60707867826173	3.6387121745033655	8.979360510657644	5.774848636577267
9	75.30848648864506	6.203826860518337	12.212133033934283	6.275553616902325
10	37.00808807492758	25.63440549731063	20.51984235472644	16.837664073035345
11	28.575970461477972	25.160732442303736	26.18241634694247	20.08088074927582
12	24.988096737430922	22.00183694219776	32.55503915021457	20.455027170156754
13	23.487824882272893	23.946599660257846	32.02146580615038	20.54410965131888
14	20.09700775017586	26.68818981326469	32.156932613710715	21.057869822848733
15	18.971650268322577	24.6884417016597	35.16423430535631	21.175673724661408
16	20.590801158686617	24.222755351860442	32.40237020836085	22.78407328109209
17	20.17426376401129	23.857824360065244	30.05212861052832	25.91578326539514
18	20.700311174322128	23.43330026018228	32.536454701558334	23.32993386393726
19	22.97729011092305	25.6003084096934	30.046292172107353	21.376109307276195
20	24.277433564435817	26.002408298801072	29.733581945131338	19.986576191631773
21	23.830331663292796	26.33262784103999	30.280824842339367	19.556215653327847
22	22.179387542582962	24.038753951115222	29.397218783501927	24.384639722799893
23	21.315133884825567	24.580621181356573	29.050104287939156	25.054140645878707
24	22.06788085064554	24.863842035258234	30.144436491870458	22.92384062222577
25	20.680498001787793	25.240599494380124	29.190025219557597	24.888877284274486
26	19.01281251823887	26.28347888591606	30.27345249907078	24.43025609677429
27	19.405696978260803	25.056598093634904	30.55867002927435	24.979034898829948
28	17.570905047290513	24.99132213761093	31.479752166393787	25.95802064870477
29	18.430858171474558	23.874258541934807	31.313260080911466	26.381623205679162
30	20.718895622978366	25.040010321280576	29.443603109900135	24.797490945840924
31	21.766229138572406	25.988277974202944	27.241115558408925	25.004377328815725
32	21.94685154865286	25.766800495175723	27.31468540061006	24.97166255556136
33	23.122586709508173	24.599973582436622	26.811522972528806	25.4659167355264
34	20.485591676624452	24.512580596606877	29.39307184041334	25.60875588635533
35	20.636263942176257	23.29675831922861	29.228883612202456	26.83809412639268
36	21.92765273805757	24.556814656218418	28.56844452772462	24.94708807799939
37	20.123271723070214	25.376066301940465	28.71481625970308	25.78584571528625
38	20.527521878964556	26.04188105338498	26.675902574483707	26.75469449316676
39	21.727677926897073	25.11004758233218	28.565679898998898	24.59659459177185
40	22.75704135577393	23.90574459131108	30.215241705345868	23.121972347569123
41	21.598047557757702	23.57076374404453	29.082051108769708	25.749137589428063
42	23.36418454203925	22.94918305221155	27.89126408040769	25.79536832534151
43	22.93305605131151	21.599583462605324	28.308569427506825	27.158791058576337
44	20.05461677638147	23.165745635726374	28.968854921499904	27.810782666392253
45	20.25551313045054	23.0035540838174	28.60346315825042	28.137469627481636
46	21.2449430332892	24.16638764395268	30.137371329571387	24.451297993186728
47	19.5787934545879	23.46693657634522	31.076730734377545	25.877539234689333
48	20.116820922710197	22.621881729183112	30.531484513471423	26.72981283463527
49	21.678068200318855	21.10333260633837	31.24783053440273	25.97076865894004
50	21.07645427150497	22.01811753358256	29.342694161411316	27.562734033501158
51	20.299440009092557	21.612485063325355	28.384596717464163	29.70347821011793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	630.0
2	1032.0
3	2196.0
4	3360.0
5	2320.0
6	1280.0
7	1301.0
8	1322.0
9	1370.0
10	1418.0
11	1524.0
12	1630.0
13	1656.5
14	1683.0
15	1681.5
16	1680.0
17	1677.0
18	1674.0
19	1823.5
20	1973.0
21	2061.5
22	2150.0
23	2316.0
24	2482.0
25	3297.0
26	5158.0
27	6204.0
28	7144.5
29	8085.0
30	8793.5
31	9502.0
32	11810.0
33	14118.0
34	15406.0
35	16694.0
36	17756.5
37	18819.0
38	20136.0
39	21453.0
40	24622.5
41	27792.0
42	30935.0
43	34078.0
44	38158.0
45	42238.0
46	45830.5
47	49423.0
48	55340.0
49	61257.0
50	67921.5
51	74586.0
52	70364.0
53	66142.0
54	54924.5
55	43707.0
56	38473.0
57	33239.0
58	29419.0
59	25599.0
60	22379.0
61	19159.0
62	17564.0
63	15969.0
64	13777.5
65	11586.0
66	9903.5
67	8221.0
68	6910.5
69	5600.0
70	4799.5
71	3999.0
72	3586.0
73	3173.0
74	2732.5
75	1594.0
76	896.0
77	779.0
78	662.0
79	471.0
80	280.0
81	220.0
82	160.0
83	126.0
84	92.0
85	55.5
86	19.0
87	14.0
88	9.0
89	6.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.376453363997946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42327224029106	21.695856983113305
2	11.781864764897017	7.157825311436499
3	4.899881044466342	4.465230241091081
4	2.528459232865799	3.0722249587967183
5	1.439262713606107	2.185984834919852
6	0.9453956167867166	1.7230659518309859
7	0.6346518251915627	1.3494930099215376
8	0.4979116260529442	1.2099831430551715
9	0.38201701154031226	1.0443889741777344
>10	4.613413057551334	34.35657647700298
>50	0.7686878113462242	14.98661555611102
>100	0.08059235181990573	4.194324856586726
>500	0.0015302345282260585	0.296872051171783
>1k	0.003060469056452117	2.261557650784627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3306	0.5077701426241241	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2930	0.4500201203535038	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2860	0.4392687864201437	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2493	0.382901078512384	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1913	0.29381859735025695	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	1094	0.16802799032994306	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	658	0.10106253897358551	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5530793356289991	0.0
2	0.0	0.0	0.0	1.9877680537935314	0.0
3	0.0	0.0	0.0	2.793350146371732	0.0
4	0.0	0.0	0.0	3.679106471995847	0.0
5	0.0	0.0	0.0	5.044986652986874	0.0
6	0.0	0.0	0.0	5.979891933734922	0.0
7	0.0	0.0	0.0	6.838001972101824	0.0
8	0.0	0.0	0.0	8.18760156170805	0.0
9	0.0	0.0	0.0	8.904408354093647	0.0
10	0.0	0.0	0.0	10.091355620336609	0.0
11	0.0	0.0	0.0	12.086188836429205	0.0
12	0.0	0.0	0.0	13.806248675282069	0.0
13	0.0	0.0	0.0	14.400643851312124	0.0
14	0.0	0.0	0.0	14.617820796765999	0.0
15	1.53590484762288E-4	0.0	0.0	14.937903367010607	0.0
16	1.53590484762288E-4	0.0	0.0	15.777121775751748	0.0
17	1.53590484762288E-4	0.0	0.0	16.866692674655418	0.0
18	1.53590484762288E-4	0.0	0.0	18.227657960134053	0.0
19	1.53590484762288E-4	0.0	0.0	19.038154948224648	0.0
20	1.53590484762288E-4	0.0	0.0	19.89826166289346	0.0
21	1.53590484762288E-4	0.0	0.0	21.004727515120983	0.0
22	1.53590484762288E-4	0.0	0.0	22.101824347778006	0.0
23	1.53590484762288E-4	0.0	0.0	23.142860653496793	0.0
24	1.53590484762288E-4	0.0	0.0	24.008035854162763	0.0
25	1.53590484762288E-4	0.0	0.0	24.724535465578835	0.0
26	1.53590484762288E-4	0.0	0.0	25.401562322411003	0.0
27	1.53590484762288E-4	0.0	0.0	25.98658847887056	0.0
28	3.07180969524576E-4	0.0	0.0	26.685885955993253	0.0
29	3.07180969524576E-4	0.0	0.0	27.394245271716926	0.0
30	3.07180969524576E-4	0.0	0.0	28.10414049228822	0.0
31	4.60771454286864E-4	0.0	0.0	28.827398085033835	0.0
32	4.60771454286864E-4	0.0	0.0	29.49152334114597	0.0
33	4.60771454286864E-4	0.0	0.0	30.17223636961243	0.0
34	4.60771454286864E-4	0.0	0.0	30.84818809305126	0.0
35	4.60771454286864E-4	0.0	0.0	31.544567350963472	0.0
36	4.60771454286864E-4	0.0	0.0	32.15585748031738	0.0
37	4.60771454286864E-4	0.0	0.0	32.81092089782854	0.0
38	4.60771454286864E-4	0.0	0.0	33.47335665860829	0.0
39	4.60771454286864E-4	0.0	0.0	34.140092952961375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	45	3.8380676E-10	45.000004	27
TCGACGT	45	3.8380676E-10	45.000004	26
TTTAGCG	40	6.8066583E-9	45.0	1
AGCAACG	20	7.0310314E-4	45.0	40
TTTACGG	85	0.0	45.0	2
CCGGATG	20	7.0310314E-4	45.0	5
ATTAACG	20	7.0310314E-4	45.0	42
TAACGGT	20	7.0310314E-4	45.0	35
CCTAACG	20	7.0310314E-4	45.0	33
AACGGTA	20	7.0310314E-4	45.0	36
AAACGTC	20	7.0310314E-4	45.0	32
TCCGTAG	20	7.0310314E-4	45.0	16
TCATCGG	25	3.8889353E-5	45.0	2
ATTATCG	20	7.0310314E-4	45.0	30
CCGTTCA	20	7.0310314E-4	45.0	25
GTAACGG	20	7.0310314E-4	45.0	34
GCCCGTT	20	7.0310314E-4	45.0	23
TAGTACG	20	7.0310314E-4	45.0	38
GTACGAG	20	7.0310314E-4	45.0	25
TTACGGG	155	0.0	42.09677	3
>>END_MODULE