##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	676858
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19297105153518	33.0	31.0	34.0	30.0	34.0
2	32.38415443121009	34.0	31.0	34.0	30.0	34.0
3	32.46126957205204	34.0	31.0	34.0	30.0	34.0
4	35.94653383723026	37.0	35.0	37.0	35.0	37.0
5	35.90144166132335	37.0	35.0	37.0	35.0	37.0
6	35.53769476020081	37.0	35.0	37.0	33.0	37.0
7	35.878426494183415	37.0	35.0	37.0	35.0	37.0
8	35.970221228086245	37.0	35.0	37.0	35.0	37.0
9	37.75825653238936	39.0	38.0	39.0	35.0	39.0
10	37.28215667097087	39.0	37.0	39.0	34.0	39.0
11	37.18016629780545	39.0	37.0	39.0	34.0	39.0
12	36.94817967727352	39.0	37.0	39.0	33.0	39.0
13	36.849213572122956	39.0	36.0	39.0	33.0	39.0
14	37.94539475044987	40.0	37.0	41.0	33.0	41.0
15	38.01267769606032	40.0	37.0	41.0	33.0	41.0
16	38.061500344237636	40.0	37.0	41.0	33.0	41.0
17	38.006525740997375	40.0	37.0	41.0	33.0	41.0
18	37.9935540393997	40.0	37.0	41.0	33.0	41.0
19	38.025449946665326	40.0	37.0	41.0	34.0	41.0
20	37.969534230222585	40.0	36.0	41.0	34.0	41.0
21	37.80695950997107	40.0	36.0	41.0	33.0	41.0
22	37.878460474722914	40.0	36.0	41.0	33.0	41.0
23	37.84118382289934	40.0	36.0	41.0	33.0	41.0
24	37.79143040342287	40.0	36.0	41.0	33.0	41.0
25	37.70483912430672	39.0	36.0	41.0	33.0	41.0
26	37.65911904712657	40.0	36.0	41.0	33.0	41.0
27	37.56503136551537	39.0	35.0	41.0	33.0	41.0
28	37.47786980430164	39.0	35.0	41.0	33.0	41.0
29	37.394782952997524	39.0	35.0	41.0	33.0	41.0
30	37.324765312665285	39.0	35.0	41.0	32.0	41.0
31	37.25644374447816	39.0	35.0	41.0	32.0	41.0
32	37.12752748730162	39.0	35.0	41.0	32.0	41.0
33	37.108712610325945	39.0	35.0	41.0	32.0	41.0
34	36.96819716986428	39.0	35.0	41.0	31.0	41.0
35	36.896668134231994	39.0	35.0	41.0	31.0	41.0
36	36.755728675734055	39.0	35.0	41.0	31.0	41.0
37	36.70916647214039	39.0	35.0	41.0	31.0	41.0
38	36.61718706139249	39.0	35.0	41.0	31.0	41.0
39	36.5874097668935	39.0	35.0	41.0	31.0	41.0
40	36.47398124865186	39.0	35.0	40.0	30.0	41.0
41	36.39168776907416	39.0	35.0	40.0	30.0	41.0
42	36.345489009511596	38.0	35.0	40.0	30.0	41.0
43	36.29336729417396	38.0	35.0	40.0	30.0	41.0
44	36.15725898194304	38.0	35.0	40.0	30.0	41.0
45	36.185583977732406	38.0	35.0	40.0	30.0	41.0
46	36.055980722692205	38.0	35.0	40.0	30.0	41.0
47	35.860871851998496	38.0	35.0	40.0	30.0	41.0
48	35.76392537282562	38.0	35.0	40.0	29.0	41.0
49	35.796775690026564	38.0	35.0	40.0	29.0	41.0
50	35.69703394212671	38.0	34.0	40.0	29.0	41.0
51	34.74736503077455	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	9.0
11	19.0
12	14.0
13	14.0
14	13.0
15	31.0
16	34.0
17	76.0
18	130.0
19	285.0
20	464.0
21	745.0
22	1015.0
23	1541.0
24	2260.0
25	3412.0
26	4615.0
27	6118.0
28	7212.0
29	8896.0
30	11234.0
31	14309.0
32	19212.0
33	26699.0
34	46791.0
35	58607.0
36	58154.0
37	86638.0
38	143399.0
39	174843.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.183211249626954	4.740728483670135	53.619666163360705	17.456394103342205
2	20.072895644285804	5.025130825077047	52.38942289224623	22.51255063839092
3	21.200901813969843	4.51202467873616	50.91570166859223	23.371371838701766
4	20.607719787606854	5.033847572164324	49.15063425415671	25.207798386072117
5	18.49723280215348	6.317720999086958	49.29290929559819	25.892136903161372
6	20.61185654893641	6.401933640438615	53.931991643742116	19.054218166882862
7	82.9665897426048	2.65609625652648	9.647961610854862	4.729352390013858
8	84.14733961924067	2.857320146914124	7.708263771721691	5.287076462123517
9	78.53124288994147	5.22605923251258	10.318707912147008	5.923989965398946
10	40.006618818127286	26.442769384420366	17.88986759408916	15.660744203363187
11	28.655936695732343	25.739667699872054	25.383315259626094	20.22108034476951
12	26.42075590448809	21.29811570521439	30.900720682920195	21.380407707377323
13	24.16149325264679	23.645875501212956	30.070118104535958	22.122513141604294
14	19.549595336097084	26.217020409007503	30.72254446279722	23.510839792098196
15	18.206329835800123	24.6645529786159	34.81734721315254	22.311769972431442
16	21.056853874815694	23.33709581625688	31.80992172656599	23.79612858236144
17	19.83946411211805	24.415608591462316	28.86824119682415	26.87668609959548
18	20.83598036811266	23.50507787453203	31.974210247939745	23.684731509415563
19	23.128041627638293	25.191251340753894	29.2937366478641	22.38697038374371
20	24.746844980778835	26.109169131486958	28.449837336634864	20.694148551099346
21	24.434814983349533	26.160878648106394	29.70490117572667	19.6994051928174
22	22.66043985592251	23.02284969668675	28.734239678041774	25.582470769348966
23	21.51928469486953	24.8526278776346	27.957858221369918	25.670229206125956
24	22.721013861105284	23.72063268809706	29.402031149812814	24.156322300984844
25	21.514852450587863	24.65820009514551	28.327507394460877	25.49944005980575
26	19.81434806118861	26.033968720174688	28.866320557635426	25.285362661001276
27	19.816416441853388	26.437155208330253	30.065833601730347	23.68059474808601
28	18.31935206498261	26.247750636027057	32.13436200798395	23.298535291006388
29	19.972431440568037	24.758368815911165	31.482378874150857	23.786820869369944
30	22.978970478298255	25.61526937703329	29.781431260323437	21.62432888434502
31	25.148701795649902	26.72554656959067	26.558746443123965	21.56700519163547
32	22.524960922379584	27.799331617562324	26.754947123325717	22.92076033673237
33	23.237961285823612	26.226180380522944	25.601824902712238	24.934033430941202
34	21.26989708328778	25.048680816360303	28.87149150929737	24.80993059105455
35	20.582160512249246	25.5142142074113	28.94166871042375	24.9619565699157
36	22.72278675881795	26.296062098697213	28.081517836828407	22.89963330565643
37	21.47156419810359	28.025819300355465	28.587236909366514	21.915379592174432
38	20.95033227057965	28.889663710852204	27.595300639129626	22.564703379438523
39	22.93996672861959	24.669428447325732	28.077233334022793	24.313371490031884
40	24.582260976452964	23.9830215495717	28.488250120409305	22.946467353566035
41	20.38994885190099	24.889120022220318	28.057435976231353	26.663495149647343
42	20.69134146305429	25.355392120651597	27.6146547724929	26.33861164380121
43	21.0837428234578	24.88143746546543	28.382319482077484	25.652500228999287
44	20.262300216589004	24.085110909526076	29.388734416967814	26.26385445691711
45	21.581040631860745	24.486672241445028	27.936878931770032	25.99540819492419
46	22.877176601295986	25.123438003244402	29.841414299601986	22.157971095857622
47	19.769434652467726	25.667865342509067	30.24666916842233	24.316030836600884
48	19.524479285167644	25.544944434430857	30.314925730359988	24.615650550041515
49	22.018207659509084	22.874960479155153	30.640400202110342	24.46643165922542
50	21.88627452139149	22.814238732496328	29.298168892145764	26.001317853966416
51	20.477855030154036	23.34182354349066	27.90053452866037	28.279786897694937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	575.5
2	1007.0
3	2068.5
4	3130.0
5	2153.5
6	1177.0
7	1167.0
8	1157.0
9	1297.0
10	1437.0
11	1472.0
12	1507.0
13	1520.0
14	1533.0
15	1524.0
16	1515.0
17	1472.0
18	1429.0
19	1511.0
20	1593.0
21	1984.5
22	2376.0
23	2079.0
24	1782.0
25	2808.0
26	4894.0
27	5954.0
28	6411.5
29	6869.0
30	8742.0
31	10615.0
32	12018.0
33	13421.0
34	15354.0
35	17287.0
36	18834.5
37	20382.0
38	22084.5
39	23787.0
40	28023.5
41	32260.0
42	37703.5
43	43147.0
44	50445.5
45	57744.0
46	65488.5
47	73233.0
48	72390.0
49	71547.0
50	62182.5
51	52818.0
52	47029.0
53	41240.0
54	37123.5
55	33007.0
56	33097.0
57	33187.0
58	30184.0
59	27181.0
60	26264.0
61	25347.0
62	22470.5
63	19594.0
64	17175.0
65	14756.0
66	12280.5
67	9805.0
68	8166.0
69	6527.0
70	5675.0
71	4823.0
72	4070.5
73	3318.0
74	2951.0
75	1959.5
76	1335.0
77	1022.5
78	710.0
79	553.5
80	397.0
81	305.5
82	214.0
83	150.5
84	87.0
85	67.5
86	48.0
87	27.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	676858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.540856130624213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.18659109789816	18.628122166282655
2	12.12174134376013	6.434427861147542
3	5.193839519880443	4.135468423880916
4	2.654278690706351	2.817873154424755
5	1.5648449075556161	2.076616177908678
6	0.9585995006914471	1.5265230860843944
7	0.7033318292308925	1.3066920224194114
8	0.5145213133540066	1.0924668923094807
9	0.36219806783545033	0.8651742128289681
>10	4.163865579428692	27.38069163020722
>50	1.4327960775350488	25.5001556839852
>100	0.1372065317576725	5.798468044985033
>500	0.0033739311087952256	0.55772869052067
>1k	0.002811609257329355	1.8795919530150702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	3313	0.4894675101720006	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	2785	0.41146001081467604	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2442	0.3607846845276262	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2230	0.3294634916038519	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1824	0.2694804523253031	No Hit
TCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	771	0.11390867803882056	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	728	0.10755579456843238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4774147605553896E-4	0.0	0.0	0.43953089126522843	0.0
2	1.4774147605553896E-4	0.0	0.0	1.7437926418835266	0.0
3	1.4774147605553896E-4	0.0	0.0	2.387206770105399	0.0
4	1.4774147605553896E-4	0.0	0.0	3.1313805849971486	0.0
5	1.4774147605553896E-4	0.0	0.0	4.291151172033129	0.0
6	1.4774147605553896E-4	0.0	0.0	5.095012543251317	0.0
7	1.4774147605553896E-4	0.0	0.0	5.8380044263346225	0.0
8	1.4774147605553896E-4	0.0	0.0	7.015208507545157	0.0
9	1.4774147605553896E-4	0.0	0.0	7.624051130370034	0.0
10	1.4774147605553896E-4	0.0	0.0	8.65247954519264	0.0
11	1.4774147605553896E-4	0.0	0.0	10.545786560844371	0.0
12	1.4774147605553896E-4	0.0	0.0	12.205514302852297	0.0
13	1.4774147605553896E-4	0.0	0.0	12.736201684843794	0.0
14	1.4774147605553896E-4	0.0	0.0	12.929004311096271	0.0
15	1.4774147605553896E-4	0.0	0.0	13.210008598553907	0.0
16	1.4774147605553896E-4	0.0	0.0	13.892574217930497	0.0
17	1.4774147605553896E-4	0.0	0.0	14.869293116133665	0.0
18	1.4774147605553896E-4	0.0	0.0	16.125391145557856	0.0
19	1.4774147605553896E-4	0.0	0.0	16.839130216382166	0.0
20	1.4774147605553896E-4	0.0	0.0	17.616398121910358	0.0
21	1.4774147605553896E-4	0.0	0.0	18.64586072706535	0.0
22	1.4774147605553896E-4	0.0	0.0	19.5967248669588	0.0
23	1.4774147605553896E-4	0.0	0.0	20.549657387517026	0.0
24	1.4774147605553896E-4	0.0	0.0	21.318060804481885	0.0
25	1.4774147605553896E-4	0.0	0.0	21.974623924072702	0.0
26	1.4774147605553896E-4	0.0	0.0	22.5840575128018	0.0
27	1.4774147605553896E-4	0.0	0.0	23.158919596133902	0.0
28	2.9548295211107793E-4	0.0	0.0	23.76864866781511	0.0
29	2.9548295211107793E-4	0.0	0.0	24.40777829323137	0.0
30	2.9548295211107793E-4	0.0	0.0	25.09241229327274	0.0
31	2.9548295211107793E-4	0.0	0.0	25.710119404660947	0.0
32	2.9548295211107793E-4	0.0	0.0	26.28261762437616	0.0
33	2.9548295211107793E-4	0.0	0.0	26.91347372713331	0.0
34	2.9548295211107793E-4	0.0	0.0	27.50872413416108	0.0
35	2.9548295211107793E-4	0.0	0.0	28.180209142833505	0.0
36	2.9548295211107793E-4	0.0	0.0	28.745172547269885	0.0
37	2.9548295211107793E-4	0.0	0.0	29.357117741091926	0.0
38	2.9548295211107793E-4	0.0	0.0	29.981916443330803	0.0
39	2.9548295211107793E-4	0.0	0.0	30.635820216352617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.031228E-4	45.000004	20
AGCGTAA	20	7.031228E-4	45.000004	12
ACAGTCG	20	7.031228E-4	45.000004	25
GTACATA	20	7.031228E-4	45.000004	20
GAATGGT	20	7.031228E-4	45.000004	10
CATGCGG	20	7.031228E-4	45.000004	2
ATTCGCT	20	7.031228E-4	45.000004	11
AGGCGAT	20	7.031228E-4	45.000004	23
TATGCGA	20	7.031228E-4	45.000004	35
CCGTCCA	20	7.031228E-4	45.000004	45
ATCGTAG	20	7.031228E-4	45.000004	27
CGTTACC	20	7.031228E-4	45.000004	25
CGTTACA	40	6.8084773E-9	45.000004	20
CTTATCA	20	7.031228E-4	45.000004	15
CATAGCA	20	7.031228E-4	45.000004	34
GTGTCGA	40	6.8084773E-9	45.000004	22
ACAATCG	20	7.031228E-4	45.000004	42
TCATGCG	20	7.031228E-4	45.000004	1
GGACGTA	20	7.031228E-4	45.000004	9
ACGTATA	20	7.031228E-4	45.000004	20
>>END_MODULE