##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1938149
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22844683251907	33.0	31.0	34.0	30.0	34.0
2	32.42001259965049	34.0	31.0	34.0	31.0	34.0
3	32.48384515328801	34.0	31.0	34.0	30.0	34.0
4	35.97239015163437	37.0	35.0	37.0	35.0	37.0
5	35.9347263806859	37.0	35.0	37.0	35.0	37.0
6	35.56514643610992	37.0	35.0	37.0	33.0	37.0
7	35.892453057014706	37.0	35.0	37.0	35.0	37.0
8	35.989376977724625	37.0	35.0	37.0	35.0	37.0
9	37.78728312425928	39.0	38.0	39.0	35.0	39.0
10	37.280906679517415	39.0	37.0	39.0	34.0	39.0
11	37.17059575914958	39.0	37.0	39.0	34.0	39.0
12	36.88128518498836	39.0	35.0	39.0	33.0	39.0
13	36.753327530545896	39.0	35.0	39.0	33.0	39.0
14	37.61585254797232	40.0	37.0	41.0	32.0	41.0
15	37.75256133558359	40.0	37.0	41.0	32.0	41.0
16	37.896713307387614	40.0	37.0	41.0	33.0	41.0
17	37.87274353003819	40.0	36.0	41.0	33.0	41.0
18	37.87441161644435	40.0	36.0	41.0	33.0	41.0
19	37.87271876414042	40.0	36.0	41.0	33.0	41.0
20	37.774101991126585	40.0	36.0	41.0	33.0	41.0
21	37.620103511133564	40.0	36.0	41.0	33.0	41.0
22	37.76981233125007	40.0	36.0	41.0	33.0	41.0
23	37.7360455775072	39.0	36.0	41.0	33.0	41.0
24	37.657260097133914	39.0	35.0	41.0	33.0	41.0
25	37.58973381303502	39.0	35.0	41.0	33.0	41.0
26	37.528922182969424	39.0	35.0	41.0	33.0	41.0
27	37.45265921247541	39.0	35.0	41.0	33.0	41.0
28	37.336900826510245	39.0	35.0	41.0	32.0	41.0
29	37.235812623281284	39.0	35.0	41.0	32.0	41.0
30	37.153249827541636	39.0	35.0	41.0	32.0	41.0
31	37.026011932003165	39.0	35.0	41.0	31.0	41.0
32	36.93187262692393	39.0	35.0	41.0	31.0	41.0
33	36.857793699039654	39.0	35.0	41.0	31.0	41.0
34	36.659457554604934	39.0	35.0	41.0	30.0	41.0
35	36.565876514137976	39.0	35.0	41.0	30.0	41.0
36	36.392188113504176	39.0	35.0	41.0	30.0	41.0
37	36.30262791973166	39.0	35.0	41.0	30.0	41.0
38	36.23730683244683	39.0	35.0	40.0	30.0	41.0
39	36.161343116550896	38.0	35.0	40.0	30.0	41.0
40	35.96716970676661	38.0	35.0	40.0	29.0	41.0
41	35.81899430848712	38.0	35.0	40.0	29.0	41.0
42	35.81375786897705	38.0	35.0	40.0	29.0	41.0
43	35.80660826386413	38.0	35.0	40.0	29.0	41.0
44	35.70040693465776	38.0	35.0	40.0	29.0	41.0
45	35.708700414674	38.0	35.0	40.0	29.0	41.0
46	35.63092672441592	38.0	34.0	40.0	29.0	41.0
47	35.3980689823125	38.0	34.0	40.0	28.0	41.0
48	35.28631441648707	37.0	34.0	40.0	27.0	41.0
49	35.27140225029139	37.0	34.0	40.0	27.0	41.0
50	35.1533457953955	37.0	34.0	40.0	27.0	41.0
51	34.17858379309331	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	36.0
10	47.0
11	53.0
12	40.0
13	29.0
14	67.0
15	85.0
16	158.0
17	292.0
18	571.0
19	984.0
20	1604.0
21	2636.0
22	3998.0
23	5960.0
24	8670.0
25	12388.0
26	16920.0
27	21250.0
28	24650.0
29	29717.0
30	36893.0
31	46481.0
32	61637.0
33	87946.0
34	140570.0
35	145959.0
36	165254.0
37	245827.0
38	399653.0
39	477617.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.14440221056276	4.479015803222559	54.09707922352719	18.279502762687493
2	20.119299393390293	4.245494025485141	53.47070839238882	22.16449818873575
3	21.193881378573064	4.730544452464697	51.68193983021945	22.39363433874279
4	18.596454658542765	5.047547943940327	50.28627829955282	26.06971909796409
5	18.356019067677458	5.9995387351540055	50.32291119000655	25.32153100716199
6	21.347326753515855	6.442641922783027	53.00309728509005	19.206934038611067
7	84.4551683074934	2.342286377363144	8.669973257989968	4.532572057153501
8	86.81577113008339	2.581019312756656	6.753402344195416	3.849807212964534
9	82.01944226166306	4.804480976436796	8.677351431701071	4.498725330199072
10	40.29488960859046	30.004401106416484	15.651170266063136	14.049539018929918
11	30.627005457268762	23.998516109958523	25.76380866486529	19.61066976790742
12	26.717295729069335	22.0253963962523	30.40514428973211	20.852163584946258
13	25.87081798148646	22.065744171371758	31.626464219211215	20.436973627930566
14	20.837149259422265	23.852758482448976	34.24535471731018	21.064737540818584
15	19.109779485478153	23.470073766258427	34.960005654879986	22.460141093383427
16	25.379472888823308	23.1110714398119	30.590630544916824	20.918825126447967
17	25.31585548892268	23.226645629412392	29.053132653887808	22.40436622777712
18	26.663017136453387	21.887687685518504	29.90002316643354	21.549272011594567
19	25.889289213574397	24.500231922313507	27.76958840625772	21.840890457854375
20	25.054059311229427	27.665468444376568	27.912147105305113	19.368325139088892
21	24.73246380954199	25.488081669675548	30.91784996922321	18.861604551559246
22	23.667994565949265	21.95558752190879	31.545923455833375	22.83049445630857
23	22.493678246615715	24.335022745929237	30.74701687021999	22.424282137235064
24	22.381818941680955	26.23183253712692	29.741366633834655	21.644981887357474
25	25.19161323510215	25.59343992644528	26.369954012823577	22.84499282562899
26	21.84924894835227	25.48163221713088	26.687989416706355	25.9811294178105
27	21.03764984013097	24.53026057336149	30.317173757022807	24.11491582948473
28	19.42802127184236	25.500309831700246	29.17350523618153	25.898163660275863
29	21.930718432896544	26.02477931263283	27.832380276232634	24.212121978238
30	25.10591290968857	23.57811499528674	29.64751420040461	21.668457894620072
31	24.63938531041731	24.95030051869077	27.950585842471348	22.45972832842057
32	25.847548356705293	23.85126220945861	27.00463173883948	23.29655769499662
33	25.372249501973275	23.747812990642103	26.646145368596528	24.23379213878809
34	21.902289246079636	24.04799630988123	30.32883436722357	23.720880076815558
35	22.102067488103337	26.121417909562165	27.78506709236493	23.991447509969564
36	24.587634903198875	26.703158529091414	25.856010038443895	22.85319652926581
37	22.716468135318802	26.955306325777844	27.19336851810671	23.134857020796648
38	25.271173681693206	25.844246237002416	26.637993260580068	22.24658682072431
39	24.176882169533922	22.824045003763903	27.257605065451624	25.741467761250554
40	25.64802809278337	22.868365641650872	29.01675774153587	22.466848524029885
41	22.100003663289044	23.383031954715555	28.3010748915589	26.215889490436496
42	22.433982113862246	23.75354010450177	29.90616304525607	23.906314736379915
43	21.794144825810605	22.966913276533436	30.244939888522502	24.994002009133457
44	23.916737051692106	23.01649666769686	28.12890030642639	24.937865974184646
45	23.23763549654851	22.508279807176848	27.067784778156888	27.18629991811775
46	22.48103731962816	23.15368942222708	28.43336606215518	25.931907195989574
47	20.938225079702335	25.437414770484622	30.19974212508945	23.42461802472359
48	21.479721115352845	26.36128594860354	29.075060792539688	23.08393214350393
49	24.35354557363753	23.67965517615003	28.758882830989773	23.20791641922267
50	22.262013911211163	23.70483383888442	28.18931877786486	25.843833472039556
51	21.92880939494332	24.078024960929216	27.017530643928822	26.975635000198643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	555.0
1	1789.0
2	3023.0
3	7849.0
4	12675.0
5	8430.5
6	4186.0
7	4133.0
8	4080.0
9	4278.5
10	4477.0
11	4428.0
12	4379.0
13	4287.5
14	4196.0
15	4156.0
16	4116.0
17	4081.0
18	4046.0
19	4297.0
20	4548.0
21	5493.5
22	6439.0
23	7766.5
24	9094.0
25	10415.0
26	14665.0
27	17594.0
28	20483.5
29	23373.0
30	27411.5
31	31450.0
32	35908.0
33	40366.0
34	46470.0
35	52574.0
36	55255.0
37	57936.0
38	63205.0
39	68474.0
40	74304.0
41	80134.0
42	84423.0
43	88712.0
44	97368.0
45	106024.0
46	113744.5
47	121465.0
48	138456.0
49	155447.0
50	167839.0
51	180231.0
52	180664.0
53	181097.0
54	166176.0
55	151255.0
56	137586.0
57	123917.0
58	111925.5
59	99934.0
60	88503.5
61	77073.0
62	68389.0
63	59705.0
64	52821.5
65	45938.0
66	39583.5
67	33229.0
68	27362.0
69	21495.0
70	17995.5
71	14496.0
72	12811.5
73	11127.0
74	10047.0
75	6734.0
76	4501.0
77	3434.0
78	2367.0
79	1600.5
80	834.0
81	692.0
82	550.0
83	366.0
84	182.0
85	126.5
86	71.0
87	62.5
88	54.0
89	35.5
90	17.0
91	12.0
92	7.0
93	5.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1938149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.643496693133688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45024780361184	18.57877689968637
2	11.114650937151174	5.700370291043427
3	4.190789305578548	3.223994750976705
4	2.1394046981566692	2.194472692098209
5	1.2661565698626551	1.6234340906131246
6	0.8505480352217045	1.3086615437135483
7	0.5774506734761516	1.036549810501346
8	0.46174423109166607	0.9472589330458158
9	0.3592846296626084	0.8291982791382175
>10	5.086274593155745	33.4298036540476
>50	1.3680966676654398	22.980499882263068
>100	0.12617892483700577	5.447159532734288
>500	0.007134501522388169	1.2757090808645795
>1k	0.0018345861057569577	1.0157581995230696
>5k	2.0384290063966195E-4	0.4083523597506288
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7812	0.4030649862317087	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	4049	0.20891066682695705	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	3541	0.18270009168541737	No Hit
TCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	3010	0.15530281727565837	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	2731	0.14090763919595448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0638248142944635E-4	0.0	0.0	0.22851700256275445	0.0
2	2.0638248142944635E-4	0.0	0.0	0.7711481418611262	0.0
3	2.0638248142944635E-4	0.0	0.0	1.085055896115314	0.0
4	2.0638248142944635E-4	0.0	0.0	1.4883272648284522	0.0
5	2.0638248142944635E-4	0.0	0.0	2.1572644827616454	0.0
6	2.0638248142944635E-4	0.0	0.0	2.8172756583730147	0.0
7	2.0638248142944635E-4	0.0	0.0	3.3878716239050766	0.0
8	2.0638248142944635E-4	0.0	0.0	4.231150443025794	0.0
9	2.0638248142944635E-4	0.0	0.0	4.716974804310711	0.0
10	2.0638248142944635E-4	0.0	0.0	5.49256016952257	0.0
11	2.0638248142944635E-4	0.0	0.0	6.793543736833443	0.0
12	3.095737221441695E-4	0.0	0.0	7.89299481102846	0.0
13	3.095737221441695E-4	0.0	0.0	8.278517286338666	0.0
14	3.611693425015311E-4	0.0	0.0	8.44780251673117	0.0
15	3.611693425015311E-4	0.0	0.0	8.643762682848429	0.0
16	4.127649628588927E-4	0.0	0.0	9.047034051561567	0.0
17	4.127649628588927E-4	0.0	0.0	9.626814037517239	0.0
18	4.127649628588927E-4	0.0	0.0	10.267012494911382	0.0
19	4.127649628588927E-4	0.0	0.0	10.722343844565097	0.0
20	4.127649628588927E-4	0.0	0.0	11.23602984084299	0.0
21	4.127649628588927E-4	0.0	0.0	11.949648866005658	0.0
22	4.127649628588927E-4	0.0	0.0	12.731322514419686	0.0
23	4.127649628588927E-4	0.0	0.0	13.433642098724093	0.0
24	4.127649628588927E-4	0.0	0.0	13.970236550440653	0.0
25	4.127649628588927E-4	0.0	0.0	14.452449218300554	0.0
26	4.643605832162543E-4	0.0	0.0	14.887503489153827	0.0
27	4.643605832162543E-4	0.0	0.0	15.38787781537952	0.0
28	4.643605832162543E-4	0.0	0.0	15.874269728488366	0.0
29	5.159562035736159E-4	0.0	0.0	16.46008640202585	0.0
30	5.159562035736159E-4	0.0	0.0	17.08800510177494	0.0
31	5.159562035736159E-4	0.0	0.0	17.575274140429865	0.0
32	5.159562035736159E-4	0.0	0.0	18.082097919200226	0.0
33	5.159562035736159E-4	0.0	0.0	18.586496703813793	0.0
34	5.159562035736159E-4	0.0	0.0	19.143780999293657	0.0
35	5.159562035736159E-4	0.0	0.0	19.784598604132086	0.0
36	5.159562035736159E-4	0.0	0.0	20.29126759604138	0.0
37	5.159562035736159E-4	0.0	0.0	20.8191939835379	0.0
38	5.675518239309775E-4	0.0	0.0	21.499327451088643	0.0
39	5.675518239309775E-4	0.0	0.0	22.804799837370606	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGTA	20	7.034488E-4	45.0	26
CGCGTAG	20	7.034488E-4	45.0	27
CGTTCAT	330	0.0	42.272728	17
TCGACGG	60	3.6379788E-12	41.250004	2
CGAACGA	105	0.0	40.714287	22
CGATTGA	50	1.0822987E-9	40.5	10
CGTTTTT	3590	0.0	39.735374	1
CACGACC	975	0.0	39.692307	27
AAACACG	905	0.0	39.530388	40
CGAATAT	115	0.0	39.130436	14
CGTTTGG	1040	0.0	38.72596	2
AACACGT	910	0.0	38.571426	41
ACACGAC	1005	0.0	38.50746	26
TCAAGCG	1030	0.0	38.4466	17
AGACACG	1030	0.0	38.228153	24
CGGGTAT	195	0.0	38.076927	6
ACGACCA	1015	0.0	37.906406	28
ACGTGAG	915	0.0	37.868855	44
ACACGTG	940	0.0	37.81915	42
GCGAGAC	1065	0.0	37.816902	21
>>END_MODULE