##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477850
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.065363607826725	33.0	31.0	34.0	30.0	34.0
2	32.27461965051795	34.0	31.0	34.0	30.0	34.0
3	32.3549461127969	34.0	31.0	34.0	30.0	34.0
4	35.86365177356911	37.0	35.0	37.0	35.0	37.0
5	35.82876634927278	37.0	35.0	37.0	35.0	37.0
6	35.49472010044993	37.0	35.0	37.0	33.0	37.0
7	35.82835617871717	37.0	35.0	37.0	35.0	37.0
8	35.92432562519619	37.0	35.0	37.0	35.0	37.0
9	37.68829339750968	39.0	38.0	39.0	35.0	39.0
10	37.17377419692372	39.0	37.0	39.0	34.0	39.0
11	37.0899173380768	39.0	37.0	39.0	33.0	39.0
12	36.81958145861672	39.0	35.0	39.0	33.0	39.0
13	36.63761222140839	39.0	35.0	39.0	33.0	39.0
14	37.68329182797949	40.0	36.0	41.0	33.0	41.0
15	37.73672700638276	39.0	36.0	41.0	33.0	41.0
16	37.83684419797007	40.0	36.0	41.0	33.0	41.0
17	37.81378047504447	39.0	36.0	41.0	33.0	41.0
18	37.78407450036622	39.0	36.0	41.0	33.0	41.0
19	37.83692999895365	39.0	36.0	41.0	33.0	41.0
20	37.799497750340066	39.0	36.0	41.0	33.0	41.0
21	37.57350842314534	39.0	35.0	41.0	33.0	41.0
22	37.66431725436853	39.0	36.0	41.0	33.0	41.0
23	37.66072407659307	39.0	36.0	41.0	33.0	41.0
24	37.597407136130585	39.0	35.0	41.0	33.0	41.0
25	37.49808726587841	39.0	35.0	41.0	33.0	41.0
26	37.37079208956786	39.0	35.0	41.0	33.0	41.0
27	37.30790206131631	39.0	35.0	41.0	32.0	41.0
28	37.21312964319347	39.0	35.0	41.0	32.0	41.0
29	37.11193680025112	39.0	35.0	41.0	32.0	41.0
30	37.12172857591295	39.0	35.0	41.0	32.0	41.0
31	37.04656272888982	39.0	35.0	40.0	32.0	41.0
32	36.86213874646856	39.0	35.0	40.0	31.0	41.0
33	36.840548289212094	39.0	35.0	40.0	31.0	41.0
34	36.6096745840745	39.0	35.0	40.0	30.0	41.0
35	36.535253740713614	39.0	35.0	40.0	30.0	41.0
36	36.403645495448366	38.0	35.0	40.0	30.0	41.0
37	36.40582400334833	38.0	35.0	40.0	30.0	41.0
38	36.33536883959402	38.0	35.0	40.0	30.0	41.0
39	36.329090718844824	38.0	35.0	40.0	30.0	41.0
40	36.16668619859789	38.0	35.0	40.0	30.0	41.0
41	36.06782044574657	38.0	35.0	40.0	30.0	41.0
42	35.98004813225908	38.0	35.0	40.0	30.0	41.0
43	35.94407031495239	38.0	35.0	40.0	30.0	41.0
44	35.821747410275194	38.0	35.0	40.0	30.0	41.0
45	35.86800878936905	38.0	35.0	40.0	30.0	41.0
46	35.750049178612535	38.0	35.0	40.0	29.0	41.0
47	35.50826828502668	38.0	34.0	40.0	28.0	41.0
48	35.394630114052525	37.0	34.0	40.0	28.0	41.0
49	35.45852464162394	37.0	34.0	40.0	28.0	41.0
50	35.31321753688396	37.0	34.0	40.0	28.0	41.0
51	34.342917233441455	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	4.0
11	3.0
12	12.0
13	6.0
14	10.0
15	13.0
16	27.0
17	48.0
18	82.0
19	143.0
20	289.0
21	490.0
22	787.0
23	1148.0
24	1762.0
25	2558.0
26	3527.0
27	4580.0
28	5509.0
29	7079.0
30	8985.0
31	11250.0
32	15128.0
33	21211.0
34	35741.0
35	45514.0
36	44253.0
37	63968.0
38	100193.0
39	103491.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.19106414146699	4.791043214397823	54.922046667364235	18.095845976770953
2	19.263785706811763	4.797530605838652	53.161033797216696	22.777649890132885
3	20.773883017683374	4.704614418750654	52.59914199016428	21.922360573401694
4	19.6547033587946	4.70691639635869	51.132572983153715	24.505807261693
5	17.283247881134248	6.008998639740504	51.337030448885635	25.370723030239617
6	20.506225803076276	6.215967353772104	53.83007219838861	19.447734644763003
7	81.43727111018102	2.8295490216595165	10.379407763942659	5.353772104216804
8	82.95762268494298	2.5962122004813226	8.294862404520247	6.151302710055457
9	77.38160510620487	5.406926859893272	11.26964528617767	5.941822747724181
10	38.934602908862615	28.01276551219002	17.80956367060793	15.243067908339436
11	27.756618185623104	24.20843360887308	27.362561473265668	20.67238673223815
12	24.65669143036518	21.76352411844721	31.542952809459035	22.036831641728575
13	24.02197342262216	25.16542848174113	31.675421157266925	19.137176938369784
14	18.409333472847127	28.829758292351155	32.233755362561475	20.527152872240244
15	15.522653552369992	24.151302710055454	39.677304593491684	20.64873914408287
16	18.198179344982734	24.4139374280632	34.24045202469394	23.147431202260123
17	19.05870042900492	23.355864811133202	29.889295804122636	27.696138955739247
18	20.40682222454745	22.02678664852987	34.66129538558125	22.905095741341423
19	22.094590352621115	23.197656168253637	31.73087789055143	22.97687558857382
20	24.197760803599454	24.86177670817202	29.995396044783927	20.945066443444595
21	22.55352097938684	26.417913571204355	31.794914722193155	19.233650727215654
22	21.93721879250811	23.587108925394997	29.165009940357855	25.310662341739036
23	20.642879564716964	24.240033483310665	28.8975619964424	26.219524955529977
24	21.3447734644763	23.641519305221305	30.314743120226012	24.698964110076385
25	20.674897980537825	25.066652715287223	28.12660876844198	26.13184053573297
26	18.145652401381188	28.256356597258552	27.864601862509154	25.733389138851102
27	18.111122737260647	28.31432457884273	28.747514910536783	24.82703777335984
28	16.151930522130375	25.193470754420844	32.53949984304698	26.1150988804018
29	18.005022496599352	24.7360050224966	31.180914512922463	26.078057967981582
30	19.833001988071572	24.533012451606155	29.010568169927804	26.623417390394476
31	22.69310453071047	26.21136339855603	25.566181856231037	25.52935021450246
32	21.461337239719576	25.76101287014754	28.118656482159675	24.658993407973213
33	21.90959506121168	24.830176833734434	27.932405566600398	25.327822538453486
34	18.959087579784452	24.802553102438004	29.694883331589413	26.543475986188135
35	17.907711624986923	25.97760803599456	29.29015381395835	26.824526525060165
36	21.90101496285445	24.15402322904677	28.391754734749398	25.553207073349377
37	19.83928010882076	24.429632729936174	30.345505911897035	25.38558124934603
38	17.88531966098148	26.11572669247672	30.01716019671445	25.98179344982735
39	19.97091137386209	23.227581877158105	32.10254263890342	24.698964110076385
40	22.166998011928428	22.837919849325104	30.934184367479332	24.060897771267133
41	19.210003139060376	23.067071256670506	30.364758815527885	27.35816678874124
42	21.351051585225488	22.454326671549648	30.452024693941617	25.74259704928325
43	20.50036622371037	21.686093962540546	30.352411844721143	27.461127969027938
44	18.99612849220467	23.105577063932195	29.594224128910746	28.30407031495239
45	20.78643925918175	23.895573924871822	27.175054933556552	28.14293188238987
46	21.442084336088733	23.20121377001151	29.4577796379617	25.898922255938057
47	19.027519095950613	24.170974155069583	31.588573820236476	25.21293292874333
48	18.738516270796275	22.18897143455059	32.92623208119703	26.14628021345611
49	21.296222664015904	19.236789787590247	34.11991210630951	25.34707544208434
50	20.66694569425552	21.012033064769277	30.719681908548708	27.601339332426495
51	19.582295699487286	20.685152244428167	28.434655226535522	31.29789682954902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	331.0
2	542.0
3	1366.0
4	2190.0
5	1461.0
6	732.0
7	752.5
8	773.0
9	935.0
10	1097.0
11	1163.5
12	1230.0
13	1235.0
14	1240.0
15	1165.5
16	1091.0
17	1276.0
18	1461.0
19	1438.0
20	1415.0
21	1705.0
22	1995.0
23	1843.0
24	1691.0
25	2277.0
26	3516.5
27	4170.0
28	4994.5
29	5819.0
30	6564.5
31	7310.0
32	7962.0
33	8614.0
34	9664.0
35	10714.0
36	12154.5
37	13595.0
38	14246.5
39	14898.0
40	18322.0
41	21746.0
42	26062.5
43	30379.0
44	34342.0
45	38305.0
46	43000.0
47	47695.0
48	54340.5
49	60986.0
50	56888.0
51	52790.0
52	43848.0
53	34906.0
54	30343.0
55	25780.0
56	22439.0
57	19098.0
58	16686.5
59	14275.0
60	13938.5
61	13602.0
62	12167.0
63	10732.0
64	9154.0
65	7576.0
66	6680.0
67	5784.0
68	4654.5
69	3525.0
70	3023.5
71	2522.0
72	2058.0
73	1594.0
74	1413.5
75	1006.0
76	779.0
77	659.5
78	540.0
79	401.5
80	263.0
81	160.0
82	57.0
83	62.5
84	68.0
85	55.5
86	43.0
87	26.0
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.364350768430803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33922744959759	20.654657476104195
2	12.17572341176937	7.150644262451815
3	5.256618527071532	4.630715708542816
4	2.733308197013881	3.21047282621371
5	1.6539233626469356	2.4283192882433595
6	1.0803020568065733	1.9033421119155287
7	0.7335433243491506	1.5078016436020485
8	0.5152283716628248	1.2103477305083694
9	0.39976119798785564	1.0564855237191157
>10	4.010671718608436	28.775176075806876
>50	0.9827841019314655	18.874129133295828
>100	0.11102881618030402	5.109188537381235
>500	0.0028652597723949425	0.4577025219160794
>1k	0.0050142046016911496	3.0310171602990366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3096	0.6479020613163127	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	2821	0.5903526211154129	TruSeq Adapter, Index 14 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2588	0.5415925499633777	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	2287	0.4786020717798472	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1464	0.3063722925604269	No Hit
TCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1096	0.2293606780370409	TruSeq Adapter, Index 14 (95% over 21bp)
TTGGGGAGAAAAGACAGGCCAAGAGGAGTCATTGAGCCAAATGAACACAAG	1058	0.22140839175473476	No Hit
TTCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	597	0.12493460290886262	No Hit
TTTTTTGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATAC	558	0.11677304593491682	No Hit
TGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	513	0.1073558648111332	TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	508	0.10630951135293502	No Hit
TTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	483	0.10107774406194414	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6399497750340065	0.0
2	0.0	0.0	0.0	2.5476614000209272	0.0
3	0.0	0.0	0.0	3.494820550381919	0.0
4	0.0	0.0	0.0	4.530082661923197	0.0
5	0.0	0.0	0.0	6.179972794810086	0.0
6	0.0	0.0	0.0	7.313173590038715	0.0
7	0.0	0.0	0.0	8.28732865962122	0.0
8	0.0	0.0	0.0	9.87925081092393	0.0
9	0.0	0.0	0.0	10.642461023333682	0.0
10	0.0	0.0	0.0	11.990582818876216	0.0
11	0.0	0.0	0.0	14.469812702730982	0.0
12	0.0	0.0	0.0	16.601025426389032	0.0
13	0.0	0.0	0.0	17.290781626033272	0.0
14	0.0	0.0	0.0	17.518886679920477	0.0
15	0.0	0.0	0.0	17.875274667782776	0.0
16	0.0	0.0	0.0	18.891283875693208	0.0
17	0.0	0.0	0.0	20.231453384953436	0.0
18	0.0	0.0	0.0	22.042272679711207	0.0
19	0.0	0.0	0.0	22.94318300721984	0.0
20	0.0	0.0	0.0	23.952495552997803	0.0
21	0.0	0.0	0.0	25.187401904363295	0.0
22	0.0	0.0	0.0	26.318091451292247	0.0
23	0.0	0.0	0.0	27.376164068222245	0.0
24	0.0	0.0	0.0	28.21847860207178	0.0
25	0.0	0.0	0.0	28.986502040389244	0.0
26	0.0	0.0	0.0	29.68881448153186	0.0
27	0.0	0.0	0.0	30.28063199748875	0.0
28	0.0	0.0	0.0	30.910118237940775	0.0
29	0.0	0.0	0.0	31.590038715077952	0.0
30	0.0	0.0	0.0	32.357015799937216	0.0
31	0.0	0.0	0.0	33.042586585748666	0.0
32	0.0	0.0	0.0	33.68839594014858	0.0
33	2.0927069163963587E-4	0.0	0.0	34.35764361201214	0.0
34	2.0927069163963587E-4	0.0	0.0	34.97331798681595	0.0
35	2.0927069163963587E-4	0.0	0.0	35.64549544836245	0.0
36	2.0927069163963587E-4	0.0	0.0	36.273098252589726	0.0
37	2.0927069163963587E-4	0.0	0.0	36.93502145024589	0.0
38	2.0927069163963587E-4	0.0	0.0	37.61284922046667	0.0
39	2.0927069163963587E-4	0.0	0.0	38.3452966412054	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.029144E-4	45.000004	29
TCGCACC	20	7.029144E-4	45.000004	31
CTACTAT	40	6.8012014E-9	45.000004	29
GTCGAAA	20	7.029144E-4	45.000004	44
CGAAATG	20	7.029144E-4	45.000004	12
GATCGAA	20	7.029144E-4	45.000004	9
CTCCGAC	20	7.029144E-4	45.000004	5
CTATCGT	20	7.029144E-4	45.000004	30
CGACATA	20	7.029144E-4	45.000004	8
ATCGTGT	20	7.029144E-4	45.000004	32
GTAATCG	20	7.029144E-4	45.000004	22
TAGCACA	20	7.029144E-4	45.000004	23
TCGGAGA	20	7.029144E-4	45.000004	29
ACCAGTC	20	7.029144E-4	45.000004	32
CGGGCAA	40	6.8012014E-9	45.000004	6
TGTTAAG	20	7.029144E-4	45.000004	33
GAACGTA	20	7.029144E-4	45.000004	18
CGAATAT	20	7.029144E-4	45.000004	14
TTAACTA	20	7.029144E-4	45.000004	21
GACGGAT	20	7.029144E-4	45.000004	21
>>END_MODULE