##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1502210
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27939569034955	33.0	31.0	34.0	30.0	34.0
2	32.471997257374134	34.0	31.0	34.0	31.0	34.0
3	32.52742892138915	34.0	31.0	34.0	31.0	34.0
4	35.9950566165849	37.0	35.0	37.0	35.0	37.0
5	35.954602219396754	37.0	35.0	37.0	35.0	37.0
6	35.591537800973235	37.0	35.0	37.0	33.0	37.0
7	35.924364769239986	37.0	35.0	37.0	35.0	37.0
8	36.01477556400237	37.0	35.0	37.0	35.0	37.0
9	37.80254757989895	39.0	38.0	39.0	35.0	39.0
10	37.31854401182258	39.0	37.0	39.0	34.0	39.0
11	37.20489678540284	39.0	37.0	39.0	34.0	39.0
12	37.083410441948864	39.0	37.0	39.0	33.0	39.0
13	37.05399511386557	39.0	37.0	39.0	33.0	39.0
14	38.26375273763322	40.0	38.0	41.0	33.0	41.0
15	38.302122872301474	40.0	38.0	41.0	33.0	41.0
16	38.33142303672589	40.0	38.0	41.0	34.0	41.0
17	38.264674712590114	40.0	37.0	41.0	34.0	41.0
18	38.230391889283126	40.0	37.0	41.0	34.0	41.0
19	38.24470946139355	40.0	37.0	41.0	34.0	41.0
20	38.2145465680564	40.0	37.0	41.0	34.0	41.0
21	38.06155464282624	40.0	37.0	41.0	33.0	41.0
22	38.11380832240499	40.0	37.0	41.0	34.0	41.0
23	38.071457386117785	40.0	37.0	41.0	34.0	41.0
24	38.0350969571498	40.0	37.0	41.0	34.0	41.0
25	37.96134961157228	40.0	37.0	41.0	33.0	41.0
26	37.889086079842365	40.0	37.0	41.0	33.0	41.0
27	37.79682068419196	40.0	36.0	41.0	33.0	41.0
28	37.62606759374521	40.0	36.0	41.0	33.0	41.0
29	37.54534119730264	40.0	36.0	41.0	33.0	41.0
30	37.51456653863308	40.0	36.0	41.0	33.0	41.0
31	37.45488646727156	40.0	36.0	41.0	32.0	41.0
32	37.34547167173697	40.0	36.0	41.0	32.0	41.0
33	37.28894894854914	40.0	36.0	41.0	32.0	41.0
34	37.15417950885695	39.0	35.0	41.0	31.0	41.0
35	37.115870617290526	39.0	35.0	41.0	31.0	41.0
36	36.96088296576377	39.0	35.0	41.0	31.0	41.0
37	36.880886826741936	39.0	35.0	41.0	31.0	41.0
38	36.77655054885802	39.0	35.0	41.0	31.0	41.0
39	36.78110450602779	39.0	35.0	41.0	31.0	41.0
40	36.70322058833319	39.0	35.0	41.0	31.0	41.0
41	36.62705081180394	39.0	35.0	41.0	30.0	41.0
42	36.55406833931341	39.0	35.0	41.0	30.0	41.0
43	36.46721031014306	39.0	35.0	41.0	30.0	41.0
44	36.32155624047237	39.0	35.0	40.0	30.0	41.0
45	36.34947111256083	39.0	35.0	40.0	30.0	41.0
46	36.256621244699474	39.0	35.0	40.0	30.0	41.0
47	36.057973252740965	38.0	35.0	40.0	29.0	41.0
48	35.94072466565926	38.0	35.0	40.0	29.0	41.0
49	35.895724965217916	38.0	35.0	40.0	29.0	41.0
50	35.81502785895447	38.0	35.0	40.0	29.0	41.0
51	34.916041698564115	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	34.0
10	46.0
11	48.0
12	29.0
13	27.0
14	47.0
15	46.0
16	107.0
17	170.0
18	322.0
19	515.0
20	907.0
21	1504.0
22	2379.0
23	3452.0
24	5446.0
25	7894.0
26	10937.0
27	13603.0
28	16376.0
29	19356.0
30	23545.0
31	30054.0
32	39034.0
33	53759.0
34	88602.0
35	109882.0
36	122540.0
37	185300.0
38	321465.0
39	444615.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.89553391336764	4.408771077279475	55.468809287649535	17.226885721703358
2	20.172279508191266	4.265116062334827	53.54091638319542	22.021688046278484
3	20.829777461207154	4.382609621823846	52.1095585836867	22.678054333282297
4	18.58621630797292	4.891393347135221	51.485677768088344	25.03671257680351
5	18.645462352134523	5.708056796320089	50.46697865145352	25.179502200091864
6	21.75361633859447	6.390983950313204	53.16480385565268	18.69059585543965
7	85.41382363318046	2.1425766038037293	8.389772401994396	4.053827361021428
8	87.06578973645496	2.563356654528994	6.3675518070043475	4.003301802011703
9	81.96577043156417	5.255989508790382	8.15698204645156	4.621258013193894
10	37.276079908934165	34.341004253732834	14.946379001604305	13.436536835728694
11	26.473329294838937	25.793264590170484	29.13187903156017	18.601527083430412
12	26.61691774119464	22.115882599636535	31.58340045666052	19.683799202508304
13	24.31144780024098	22.334760120089733	32.03040853143036	21.32338354823893
14	19.8320474500902	26.177232211208818	31.507645402440403	22.483074936260575
15	18.39350024297535	26.343187703450248	34.316972993123464	20.946339060450935
16	21.61814926009013	24.274302527609322	33.90371519294906	20.20383301935149
17	21.07501614288282	24.349059052995255	31.21467704249073	23.361247761631194
18	21.573148893962895	25.195811504383542	30.719739583680045	22.511300017973518
19	23.211734710859332	26.63269449677475	29.607045619454002	20.548525172911912
20	27.106330007122835	25.95755586768827	28.510527822341754	18.425586302847137
21	25.577515793397726	26.290265675238484	28.67661645176107	19.45560207960272
22	23.129921915045166	24.769772535131573	29.019910664953635	23.080394884869627
23	22.605161728386843	26.019997204119267	28.56724426012342	22.807596807370476
24	22.9234927207248	24.491316127572045	30.216347914073268	22.368843237629893
25	23.588779198647323	26.117054206801978	27.101337362951917	23.19282923159878
26	22.31365787739397	27.753043848729536	27.557931314529927	22.37536695934656
27	22.39227538093875	26.43498578760626	29.12808462199027	22.044654209464724
28	20.621417777807363	27.39963121001724	30.52262999181206	21.45632102036333
29	23.09836840388494	26.00169084215922	29.591934549763348	21.30800620419249
30	23.72324774831748	24.483660739843298	30.21242036732548	21.58067114451375
31	22.69582814653078	26.643611745361834	28.16610194313711	22.494458164970276
32	23.64190093262593	27.086226293261262	27.747784930202833	21.52408784390997
33	23.526204725038443	26.745594823626522	27.632155291204292	22.09604516013074
34	21.84408305097157	26.589225208193263	30.128211102309265	21.438480638525906
35	22.505242276379466	24.834610340764605	29.07496288801166	23.585184494844263
36	22.507039628281	27.625631569487624	27.848037225154936	22.01929157707644
37	22.47848170362333	27.772348739523768	29.013386943236963	20.73578261361594
38	21.47409483361181	27.785263045779217	28.200717609388832	22.539924511220136
39	21.438813481470635	26.056942770984083	28.97484373023745	23.529400017307832
40	22.53726176766231	24.15853975143289	31.737506740069634	21.56669174083517
41	19.56271093921622	25.64015683559556	30.672209611172875	24.12492261401535
42	22.119211029083814	27.42273051038137	27.05846719167094	23.399591268863873
43	22.21853136379068	26.531643378755298	28.553930542334292	22.695894715119724
44	22.29528494684498	25.78068312685976	28.82146970130674	23.102562224988517
45	22.86358099067374	23.85924737553338	28.64765911556973	24.62951251822315
46	22.22006244133643	25.17683945653404	29.20903202614814	23.39406607598139
47	20.944208865604676	26.095685689750436	30.183995579845696	22.776109864799196
48	20.976894042776976	24.90097922394339	30.428036026920335	23.694090706359297
49	21.32211874504896	25.08730470440218	30.57235672775444	23.018219822794418
50	20.49879843696953	24.51614621124876	29.849355283216063	25.135700068565647
51	19.36273889802358	24.516945034316105	29.10658296776083	27.013733099899483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	1212.5
2	2055.0
3	4876.0
4	7697.0
5	5390.5
6	3084.0
7	3073.0
8	3062.0
9	2983.0
10	2904.0
11	2925.0
12	2946.0
13	2973.5
14	3001.0
15	2992.0
16	2983.0
17	3022.5
18	3062.0
19	3652.0
20	4242.0
21	4575.0
22	4908.0
23	6123.5
24	7339.0
25	9168.0
26	14207.0
27	17417.0
28	21739.0
29	26061.0
30	29663.0
31	33265.0
32	37218.0
33	41171.0
34	44427.0
35	47683.0
36	52879.5
37	58076.0
38	62460.5
39	66845.0
40	74733.0
41	82621.0
42	89701.0
43	96781.0
44	106385.5
45	115990.0
46	125520.0
47	135050.0
48	133708.0
49	132366.0
50	118046.5
51	103727.0
52	94531.0
53	85335.0
54	79294.5
55	73254.0
56	72129.5
57	71005.0
58	68956.5
59	66908.0
60	63580.0
61	60252.0
62	51937.0
63	43622.0
64	36524.5
65	29427.0
66	24423.0
67	19419.0
68	15866.0
69	12313.0
70	10512.0
71	8711.0
72	7646.0
73	6581.0
74	5621.0
75	3320.0
76	1979.0
77	1846.5
78	1714.0
79	1212.0
80	710.0
81	502.0
82	294.0
83	254.5
84	215.0
85	143.0
86	71.0
87	43.0
88	15.0
89	17.0
90	19.0
91	10.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1502210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.88087140982371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81865238641277	18.84610178649401
2	11.0087992691328	5.698346365219744
3	4.26498925861439	3.3114491569943514
4	2.059982852808878	2.1325660527995347
5	1.1838073905481206	1.5318983424387433
6	0.7594171371733477	1.1792626364159917
7	0.5424963519379269	0.9828194827362768
8	0.4125211750383589	0.8541125987997716
9	0.32562789088563027	0.7584780214316792
>10	5.147889216444653	35.02084404291811
>50	1.3594396626921676	22.714082202740077
>100	0.11066244631383487	5.051938925593645
>500	0.003896564998107827	0.6442746580252761
>1k	0.0018183969991169859	1.2738257273927691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4275	0.28458071774252597	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3485	0.23199153247548612	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	3232	0.2151496794722442	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	3122	0.207827134688226	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	2539	0.16901764733292948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6627435578248045E-4	0.0	0.0	0.22679918253772774	0.0
2	2.6627435578248045E-4	0.0	0.0	0.9195784876947963	0.0
3	2.6627435578248045E-4	0.0	0.0	1.3225847251715805	0.0
4	2.6627435578248045E-4	0.0	0.0	1.790362199692453	0.0
5	2.6627435578248045E-4	0.0	0.0	2.5408564714653745	0.0
6	2.6627435578248045E-4	0.0	0.0	3.185573255403705	0.0
7	2.6627435578248045E-4	0.0	0.0	3.7290392155557477	0.0
8	2.6627435578248045E-4	0.0	0.0	4.5363164936992835	0.0
9	2.6627435578248045E-4	0.0	0.0	4.988250644051098	0.0
10	2.6627435578248045E-4	0.0	0.0	5.734417957542554	0.0
11	3.328429447281006E-4	0.0	0.0	7.089088742585924	0.0
12	3.328429447281006E-4	0.0	0.0	8.235000432695829	0.0
13	3.9941153367372074E-4	0.0	0.0	8.631616085633834	0.0
14	3.9941153367372074E-4	0.0	0.0	8.784457565852977	0.0
15	3.9941153367372074E-4	0.0	0.0	8.982632255144088	0.0
16	3.9941153367372074E-4	0.0	0.0	9.449677475186558	0.0
17	3.9941153367372074E-4	0.0	0.0	10.103846998755168	0.0
18	3.9941153367372074E-4	0.0	0.0	10.826582168937765	0.0
19	3.9941153367372074E-4	0.0	0.0	11.349012454982992	0.0
20	3.9941153367372074E-4	0.0	0.0	11.88508930176207	0.0
21	3.9941153367372074E-4	0.0	0.0	12.573009099926109	0.0
22	3.9941153367372074E-4	0.0	0.0	13.378355888990221	0.0
23	3.9941153367372074E-4	0.0	0.0	14.112407719293573	0.0
24	3.9941153367372074E-4	0.0	0.0	14.69168758029836	0.0
25	3.9941153367372074E-4	0.0	0.0	15.168651520093729	0.0
26	4.6598012261934085E-4	0.0	0.0	15.599150584805054	0.0
27	4.6598012261934085E-4	0.0	0.0	16.032911510374714	0.0
28	4.6598012261934085E-4	0.0	0.0	16.50355143422025	0.0
29	4.6598012261934085E-4	0.0	0.0	17.015264177445232	0.0
30	4.6598012261934085E-4	0.0	0.0	17.572243561153233	0.0
31	4.6598012261934085E-4	0.0	0.0	18.08968120302754	0.0
32	4.6598012261934085E-4	0.0	0.0	18.591475226499625	0.0
33	4.6598012261934085E-4	0.0	0.0	19.08854288015657	0.0
34	4.6598012261934085E-4	0.0	0.0	19.574360442281705	0.0
35	4.6598012261934085E-4	0.0	0.0	20.08527436243934	0.0
36	4.6598012261934085E-4	0.0	0.0	20.611033077931847	0.0
37	4.6598012261934085E-4	0.0	0.0	21.14384806385259	0.0
38	4.6598012261934085E-4	0.0	0.0	21.658556393580124	0.0
39	4.6598012261934085E-4	0.0	0.0	22.202222059499004	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.033978E-4	45.000004	39
CCGATAG	20	7.033978E-4	45.000004	10
CGCGCAT	20	7.033978E-4	45.000004	26
TCTAGCG	40	6.8175723E-9	45.000004	1
GAACGTA	20	7.033978E-4	45.000004	9
CGTTTAA	40	6.8175723E-9	45.000004	41
ATCGATA	20	7.033978E-4	45.000004	44
GCGATCG	35	1.2122655E-7	45.0	9
CACGTTA	25	3.8913804E-5	45.0	29
CTTTGCG	525	0.0	40.714287	1
TACGGGA	225	0.0	40.0	4
CTACGGG	155	0.0	39.19355	3
CGTTTTT	2385	0.0	38.867924	1
CAACCCG	220	0.0	38.863636	23
TTTGGGA	9880	0.0	38.737347	4
TTCGGGA	1540	0.0	38.57143	4
CGTTATG	35	6.249571E-6	38.571426	2
CATACGA	175	0.0	38.571426	18
TTGGGAT	6255	0.0	38.129498	5
CGTTTGG	1060	0.0	37.995285	2
>>END_MODULE