FastQCFastQC Report
Sat 14 Jan 2017
SRR2934318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477720
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT39510.26737135587256045No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.25458138212922615No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC28750.19455647889992692No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC25860.17499932328181253No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC23340.15794602495736676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG207.0339424E-445.01
TCATACG750.045.031
GTCGCGA302.1659253E-644.99999617
TCTAGCG750.042.01
GCGCGAC6600.041.5909089
CCATACG556.184564E-1140.9090922
TATCCGG556.184564E-1140.9090922
GACCGAT6300.040.7142879
TTTGTCG4050.040.01
CGTTTTT21450.039.9650341
CGTTAGG850.039.7058832
AGCTACG1550.039.1935469
GCGATGT4250.038.6470579
TTTCGCG4950.038.6363641
TTCGTCG1400.038.5714261
CGCGATT356.249502E-638.57142616
GGACCGA7050.038.297878
CGCGACC7200.038.12510
TGGGCGA18550.037.964966
GTTTGCG2550.037.9411771
Produced by FastQC (version 0.11.5)