##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1539937
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38504432324179	33.0	31.0	34.0	31.0	34.0
2	32.56122360849827	34.0	31.0	34.0	31.0	34.0
3	32.60790408958289	34.0	31.0	34.0	31.0	34.0
4	36.076295328964754	37.0	35.0	37.0	35.0	37.0
5	35.141435656133986	37.0	35.0	37.0	35.0	37.0
6	25.867613415354004	37.0	0.0	37.0	0.0	37.0
7	30.984370139817408	37.0	19.0	37.0	17.0	37.0
8	34.73554177865718	37.0	32.0	37.0	32.0	37.0
9	37.261616546650934	39.0	37.0	39.0	35.0	39.0
10	37.43473401833971	39.0	37.0	39.0	35.0	39.0
11	37.471113428666236	39.0	37.0	39.0	35.0	39.0
12	37.092688207374714	39.0	37.0	39.0	34.0	39.0
13	37.05986348792191	39.0	37.0	39.0	33.0	39.0
14	37.94739005556721	40.0	37.0	41.0	33.0	41.0
15	38.16680162889781	40.0	37.0	41.0	33.0	41.0
16	38.337974865205524	40.0	37.0	41.0	33.0	41.0
17	38.364813625492474	40.0	37.0	41.0	34.0	41.0
18	38.34411862303458	40.0	37.0	41.0	34.0	41.0
19	38.32876474816827	40.0	37.0	41.0	34.0	41.0
20	38.23409139464796	40.0	37.0	41.0	34.0	41.0
21	38.12855136281549	40.0	36.0	41.0	34.0	41.0
22	38.161593623635255	40.0	36.0	41.0	34.0	41.0
23	38.13125601891506	40.0	36.0	41.0	34.0	41.0
24	38.083206001284466	40.0	36.0	41.0	34.0	41.0
25	37.94121253012298	40.0	36.0	41.0	34.0	41.0
26	37.88928443176572	40.0	35.0	41.0	34.0	41.0
27	37.85185887474618	40.0	35.0	41.0	34.0	41.0
28	37.751886603153245	40.0	35.0	41.0	34.0	41.0
29	37.706449030057726	40.0	35.0	41.0	33.0	41.0
30	37.45730766907997	40.0	35.0	41.0	33.0	41.0
31	37.410131713180476	39.0	35.0	41.0	33.0	41.0
32	37.32904462974784	39.0	35.0	41.0	33.0	41.0
33	37.23463752088559	39.0	35.0	41.0	33.0	41.0
34	37.06977947799163	39.0	35.0	41.0	32.0	41.0
35	36.99121132877514	39.0	35.0	41.0	32.0	41.0
36	36.89854390147129	39.0	35.0	41.0	32.0	41.0
37	36.798587864308736	39.0	35.0	41.0	31.0	41.0
38	36.69251534316014	39.0	35.0	41.0	31.0	41.0
39	36.63698255188362	39.0	35.0	41.0	31.0	41.0
40	36.51273396249327	39.0	35.0	41.0	31.0	41.0
41	36.37175806542735	39.0	35.0	41.0	31.0	41.0
42	36.32019946270529	39.0	35.0	40.0	31.0	41.0
43	36.2352966387586	38.0	35.0	40.0	31.0	41.0
44	36.096997474572014	38.0	35.0	40.0	30.0	41.0
45	36.082397526652066	38.0	35.0	40.0	30.0	41.0
46	36.058982932418665	38.0	35.0	40.0	30.0	41.0
47	35.972763171480395	38.0	35.0	40.0	30.0	41.0
48	35.90340838618723	38.0	35.0	40.0	30.0	41.0
49	35.85677790714815	38.0	35.0	40.0	30.0	41.0
50	35.74605909202779	37.0	35.0	40.0	30.0	41.0
51	34.66689221701927	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	11.0
10	25.0
11	12.0
12	10.0
13	15.0
14	16.0
15	49.0
16	78.0
17	159.0
18	309.0
19	574.0
20	996.0
21	1697.0
22	2491.0
23	3846.0
24	6040.0
25	8953.0
26	11924.0
27	13949.0
28	15905.0
29	19172.0
30	24261.0
31	32666.0
32	46814.0
33	77645.0
34	113070.0
35	128237.0
36	151449.0
37	244970.0
38	320232.0
39	314129.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.735946990039203	4.330761583103724	53.84928084720349	17.084010579653583
2	21.69387448967068	4.115428098681959	51.61847530126232	22.572222110385034
3	22.02849856844793	4.2497193066989105	51.03572418871681	22.686057936136347
4	19.65437547120434	4.694477761103214	50.46232410806416	25.188822659628286
5	18.079246099028726	8.100136564028269	49.07298155703772	24.747635779905284
6	16.60613388729539	31.664152494550102	38.282150503559556	13.44756311459495
7	83.35847505449898	2.1664522639562525	9.944757480338481	4.530315201206283
8	84.668593585322	2.487179670337163	8.516257483260679	4.3279692610801614
9	78.79504161533882	4.595447735848934	10.99804732271515	5.611463326097107
10	45.03190714944832	22.48611469170492	17.836054332092807	14.64592382675395
11	33.5529310614655	22.228571688322315	25.16265275787256	19.05584449233962
12	30.27604376023175	19.57437219834318	29.97635617560978	20.173227865815292
13	27.90971318956555	19.693662792698664	31.834614013430418	20.56201000430537
14	21.690822416761204	20.994754980236205	35.308847050236466	22.005575552766118
15	19.86776082398176	21.388732136444546	36.424866731561096	22.3186403080126
16	26.06983272692324	21.863881444500652	30.635603924056632	21.43068190451947
17	25.778587046093442	22.722812686493018	28.379277853574532	23.119322413839008
18	26.19451315216142	21.633936972746287	30.536508961080877	21.635040914011416
19	25.471756312108873	24.060464811222797	28.34245816549638	22.125320711171952
20	25.175640302168205	27.27760940869659	27.892699506538253	19.65405078259695
21	25.082779360454353	25.338309294471138	30.586900632947973	18.992010712126536
22	24.056113983883755	21.660366625387923	31.816496389138	22.467023001590324
23	22.992044479741704	22.89061175879273	30.65203316759062	23.465310593874943
24	23.190753907465044	24.149429489647954	29.702254053250233	22.95756254963677
25	24.612110755180243	24.19176888405175	26.774601818126325	24.421518542641678
26	22.672291139182967	24.658216537429777	27.04019709897223	25.629295224415028
27	22.680343416646267	23.402256066319595	30.520209592989843	23.397190924044295
28	20.250308941209934	24.690230834118537	28.681887635663017	26.377572589008512
29	22.300133057391307	25.925735922963085	27.448005989855428	24.32612502979018
30	25.980348546726262	23.509987746251955	28.039913321129372	22.46975038589241
31	25.050310499715252	25.025504290110568	27.36436620459149	22.559819005582696
32	26.55011211497613	25.344673191176003	25.14518451079492	22.960030183052943
33	27.452421754915946	24.243719061234323	24.879394416784585	23.424464767065146
34	23.189325277592527	25.324997061568105	28.37395296041332	23.111724700426056
35	23.35615028407006	28.1414759175213	25.395649302536405	23.106724495872232
36	26.00970039683442	28.441812879358054	23.84006618452573	21.708420539281803
37	24.174690263302978	28.467528217063425	24.783286588996823	22.574494930636774
38	23.866430899445888	27.191372114573518	25.637996879093105	23.30420010688749
39	23.764153988117695	24.98433377469338	25.437599070611327	25.813913166577596
40	26.535825816250924	24.013579776315524	26.818629593288556	22.631964814144993
41	23.33835734838503	24.66730781843673	26.041000378586915	25.953334454591324
42	23.381670808611002	24.277876302731865	27.935168776385005	24.405284112272124
43	23.371215835453008	22.965095325328242	29.11171041412733	24.551978425091416
44	23.715450697009032	23.213676923146856	27.077341475657768	25.99353090418634
45	22.946588074706952	23.170428400642365	26.132952192200072	27.750031332450614
46	22.965809640264503	23.641291819080912	27.625480782655394	25.76741775799919
47	21.05099104703634	25.45870383009175	29.401397589641654	24.088907533230255
48	21.42269456477765	26.237696736944432	28.25122066681949	24.08838803145843
49	24.28417526171525	23.69811232537435	28.013483668487737	24.00422874442266
50	22.08447488436215	24.115142372707453	27.39956244963268	26.400820293297713
51	21.104694542698823	24.43703865807497	25.945671803456893	28.51259499576931
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	339.0
1	1026.0
2	1713.0
3	8464.5
4	15216.0
5	9559.5
6	3903.0
7	3739.0
8	3575.0
9	3603.0
10	3631.0
11	3577.5
12	3524.0
13	3524.5
14	3525.0
15	3483.0
16	3441.0
17	3296.5
18	3152.0
19	3321.5
20	3491.0
21	3847.0
22	4203.0
23	4934.5
24	5666.0
25	6819.0
26	8953.0
27	9934.0
28	12052.0
29	14170.0
30	15995.0
31	17820.0
32	21727.5
33	25635.0
34	29127.0
35	32619.0
36	34405.0
37	36191.0
38	40320.0
39	44449.0
40	49826.0
41	55203.0
42	62043.5
43	68884.0
44	78619.5
45	88355.0
46	103840.5
47	119326.0
48	136661.5
49	153997.0
50	154274.0
51	154551.0
52	140541.0
53	126531.0
54	115658.0
55	104785.0
56	98144.0
57	91503.0
58	88020.0
59	84537.0
60	76256.0
61	67975.0
62	60218.5
63	52462.0
64	46051.5
65	39641.0
66	34737.5
67	29834.0
68	26145.5
69	22457.0
70	18272.0
71	14087.0
72	11937.0
73	9787.0
74	7901.0
75	4484.0
76	2953.0
77	2270.5
78	1588.0
79	1151.0
80	714.0
81	530.0
82	346.0
83	250.5
84	155.0
85	101.0
86	47.0
87	30.5
88	14.0
89	9.5
90	5.0
91	7.0
92	9.0
93	6.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1539937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.5016241357239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73175338927442	25.093636181689092
2	10.366734696838204	7.153383680501589
3	3.758683046330219	3.890420091294088
4	1.929077700373527	2.662252549875762
5	1.2921898037149713	2.2291323459894397
6	0.9331113117460672	1.9316313452793061
7	0.8116378306475807	1.9601976357136013
8	0.7069569521739514	1.951293043523409
9	0.6182610735607399	1.9197910059967616
>10	6.6777182355964975	42.531007999001815
>50	0.11460497341674997	2.7535132805221103
>100	0.0542266491863451	3.368285457545064
>500	0.0027323492845959243	0.6540196396109633
>1k	0.0021018071419968646	1.3836732169216976
>5k	2.101807141996865E-4	0.5177625265353452
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7140	0.4636553313544645	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	2859	0.18565694570622043	No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	2792	0.18130611836717994	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2765	0.1795527998872681	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2131	0.13838228447007897	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	2018	0.13104432194304053	No Hit
TCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	1697	0.1101993133485331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19883930316629836	0.0
2	0.0	0.0	0.0	0.6684039671752805	0.0
3	0.0	0.0	0.0	0.9999759730430531	0.0
4	0.0	0.0	0.0	1.374601688250883	0.0
5	0.0	0.0	0.0	2.1132682700655936	0.0
6	0.0	0.0	0.0	2.8531037308669123	0.0
7	0.0	0.0	0.0	3.7109310315941495	0.0
8	0.0	0.0	0.0	5.011633592802823	0.0
9	0.0	0.0	0.0	5.804133545722975	0.0
10	0.0	0.0	0.0	6.94307624272941	0.0
11	0.0	0.0	0.0	8.811074738771781	0.0
12	0.0	0.0	0.0	10.225743001174724	0.0
13	0.0	0.0	0.0	10.750180039832799	0.0
14	0.0	0.0	0.0	11.022593781433915	0.0
15	0.0	0.0	0.0	11.31403427542815	0.0
16	0.0	0.0	0.0	11.861199516603602	0.0
17	0.0	0.0	0.0	12.564864666541553	0.0
18	0.0	0.0	0.0	13.306258632658349	0.0
19	0.0	0.0	0.0	13.860437147753448	0.0
20	0.0	0.0	0.0	14.498060634948054	0.0
21	0.0	0.0	0.0	15.371018424779715	0.0
22	0.0	0.0	0.0	16.279821836867352	0.0
23	0.0	0.0	0.0	17.13219436899042	0.0
24	6.493772147821631E-5	0.0	0.0	17.793585062246052	0.0
25	6.493772147821631E-5	0.0	0.0	18.428351289695616	0.0
26	6.493772147821631E-5	0.0	0.0	18.978503666059066	0.0
27	6.493772147821631E-5	0.0	0.0	19.617490845404713	0.0
28	6.493772147821631E-5	0.0	0.0	20.173422678979726	0.0
29	6.493772147821631E-5	0.0	0.0	20.831046984389623	0.0
30	6.493772147821631E-5	0.0	0.0	21.67536723904939	0.0
31	6.493772147821631E-5	0.0	0.0	22.301431811820873	0.0
32	6.493772147821631E-5	0.0	0.0	22.905742247897155	0.0
33	6.493772147821631E-5	0.0	0.0	23.490766180694404	0.0
34	6.493772147821631E-5	0.0	0.0	24.158780521540816	0.0
35	6.493772147821631E-5	0.0	0.0	24.880433420328234	0.0
36	6.493772147821631E-5	0.0	0.0	25.49883534196529	0.0
37	6.493772147821631E-5	0.0	0.0	26.123276471699818	0.0
38	6.493772147821631E-5	0.0	0.0	26.86226774212192	0.0
39	6.493772147821631E-5	0.0	0.0	28.22920677923837	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	20	7.034034E-4	45.0	26
CGTTAGG	410	0.0	43.353657	2
TCGTTAG	220	0.0	42.954548	1
CGTTTAT	1320	0.0	42.78409	1
TTTAGGG	5755	0.0	41.403126	3
TTAGGGA	2535	0.0	41.094677	4
CTTTAGG	1670	0.0	41.092815	2
CGTTTTT	2685	0.0	40.223465	1
TAGGGAC	1090	0.0	40.04587	5
TTTTAGG	3250	0.0	39.807693	2
TGTAGGG	1795	0.0	39.735374	3
CCGTTAG	85	0.0	39.705883	1
CTAGGGA	1555	0.0	39.501606	4
TTAGGGG	5810	0.0	39.38468	4
TTAGGGT	895	0.0	39.217876	4
GTTAGGG	2065	0.0	39.116222	3
TAGGGGA	2935	0.0	39.097103	5
TAGGGTG	505	0.0	38.76238	5
CTTAGGG	2610	0.0	38.706894	3
TAGGGGG	4750	0.0	38.652634	5
>>END_MODULE