##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675971
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34187709236047	33.0	31.0	34.0	31.0	34.0
2	32.52433314446922	34.0	31.0	34.0	31.0	34.0
3	32.564743162058726	34.0	31.0	34.0	31.0	34.0
4	36.03927535352848	37.0	35.0	37.0	35.0	37.0
5	35.24533596855486	37.0	35.0	37.0	35.0	37.0
6	26.659874462070118	37.0	15.0	37.0	0.0	37.0
7	31.39499031763197	37.0	19.0	37.0	17.0	37.0
8	34.8141192447605	37.0	32.0	37.0	32.0	37.0
9	37.29727310787001	39.0	37.0	39.0	35.0	39.0
10	37.369039500215244	39.0	37.0	39.0	34.0	39.0
11	37.42642213941131	39.0	37.0	39.0	35.0	39.0
12	37.17753720203973	39.0	37.0	39.0	34.0	39.0
13	37.18869448541432	39.0	37.0	39.0	34.0	39.0
14	38.37557380420166	40.0	38.0	41.0	34.0	41.0
15	38.51128820615086	40.0	38.0	41.0	34.0	41.0
16	38.578299956654945	40.0	38.0	41.0	35.0	41.0
17	38.55752095873935	40.0	38.0	41.0	35.0	41.0
18	38.53580553011889	40.0	38.0	41.0	35.0	41.0
19	38.5407909510911	40.0	37.0	41.0	35.0	41.0
20	38.468054102912696	40.0	37.0	41.0	34.0	41.0
21	38.34951943204664	40.0	37.0	41.0	34.0	41.0
22	38.328151651476176	40.0	37.0	41.0	34.0	41.0
23	38.2701669154446	40.0	37.0	41.0	34.0	41.0
24	38.21799899699839	40.0	37.0	41.0	34.0	41.0
25	38.10669688492553	40.0	37.0	41.0	34.0	41.0
26	38.03027496741724	40.0	36.0	41.0	34.0	41.0
27	37.97235532293545	40.0	36.0	41.0	34.0	41.0
28	37.89492300705208	40.0	36.0	41.0	34.0	41.0
29	37.85624827100571	40.0	36.0	41.0	34.0	41.0
30	37.60952023089748	40.0	36.0	41.0	33.0	41.0
31	37.649307440703815	40.0	36.0	41.0	33.0	41.0
32	37.55300301344288	40.0	36.0	41.0	33.0	41.0
33	37.44552059185971	40.0	36.0	41.0	33.0	41.0
34	37.392028652116736	40.0	36.0	41.0	33.0	41.0
35	37.317020996462865	40.0	35.0	41.0	33.0	41.0
36	37.1960261608856	39.0	35.0	41.0	32.0	41.0
37	37.10008121650189	39.0	35.0	41.0	32.0	41.0
38	37.01723002909888	39.0	35.0	41.0	32.0	41.0
39	36.99733864322582	39.0	35.0	41.0	32.0	41.0
40	36.96350878957825	39.0	35.0	41.0	32.0	41.0
41	36.86391723905315	39.0	35.0	41.0	31.0	41.0
42	36.80317350892272	39.0	35.0	41.0	31.0	41.0
43	36.69248680786602	39.0	35.0	41.0	31.0	41.0
44	36.55461254994667	39.0	35.0	41.0	31.0	41.0
45	36.52442930244049	39.0	35.0	41.0	31.0	41.0
46	36.451060770358495	39.0	35.0	41.0	31.0	41.0
47	36.37369798408512	39.0	35.0	40.0	31.0	41.0
48	36.33371993768964	39.0	35.0	40.0	31.0	41.0
49	36.30647172733742	39.0	35.0	40.0	31.0	41.0
50	36.22120327647192	39.0	35.0	40.0	31.0	41.0
51	35.19517109461796	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	6.0
11	6.0
12	5.0
13	4.0
14	7.0
15	8.0
16	21.0
17	43.0
18	102.0
19	185.0
20	350.0
21	613.0
22	970.0
23	1544.0
24	2359.0
25	3609.0
26	4867.0
27	5886.0
28	6616.0
29	7759.0
30	9598.0
31	12575.0
32	17216.0
33	27392.0
34	42229.0
35	56288.0
36	62611.0
37	105134.0
38	148801.0
39	159063.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.728933637685643	4.5749004025320605	53.215596527069955	19.48056943271235
2	20.907109920395996	4.528300770299317	52.82253232757027	21.74205698173442
3	20.736096666868846	4.383620007367179	52.47473634223954	22.40554698352444
4	19.149194270168397	4.849468394354195	51.5547264601588	24.446610875318616
5	17.917336690479324	7.8126132629950105	49.533190033300244	24.73686001322542
6	16.74287802287376	30.03501629507775	39.41914667936938	13.802959002679108
7	82.44051889800006	2.1468376601954815	10.67767700093643	4.734966440868026
8	82.98477301541043	2.9098881460891075	8.857036766370156	5.248302072130313
9	77.36249040269479	4.742067337208253	11.921221472518791	5.974220787578165
10	39.04220743197563	24.994415440899093	19.95100381525243	16.012373311872846
11	29.18705092378223	25.501685723204105	25.398870661611223	19.91239269140244
12	27.526476727551923	21.59708034812144	31.237878548044222	19.638564376282414
13	24.18328596936851	24.009905750394616	31.861426007920457	19.94538227231642
14	18.843855727538607	27.404134201023417	31.103109452920318	22.648900618517658
15	18.336733380574017	25.68083542045443	35.20994243835904	20.77248876061251
16	19.586047330432816	24.18432151675146	33.81062205331294	22.41900909950279
17	19.042828760405403	24.453563836318423	30.87099890379913	25.63260849947705
18	20.24199262986134	24.471316077168993	32.692970556429195	22.593720736540472
19	22.318413068016234	25.87951258264038	29.50378048762447	22.29829386171892
20	25.06069047340788	25.07178562393949	30.004097808929675	19.863426093722953
21	24.167161017262575	26.166359207717495	29.754974695660017	19.911505079359916
22	21.99088422432323	24.732126082331934	28.971805003469083	24.30518468987575
23	21.81336181581754	25.80717220117431	28.4211896664206	23.958276316587547
24	22.47892291237346	23.756048706231482	29.978800865717613	23.786227515677446
25	21.39721970321212	25.449612483375766	28.2781361922331	24.875031621179016
26	21.16510915409093	26.531315692537106	28.88319173455666	23.420383418815305
27	21.1096334014329	26.29402740650117	30.57409267557336	22.022246516492572
28	19.939760729380403	25.175488297574894	32.27875160324925	22.605999369795448
29	21.390562612893156	23.154543612078033	32.852296918063054	22.602596856965757
30	22.148435361872032	23.390796350731023	31.900185067110865	22.560583220286077
31	22.941812592552047	25.72521602258085	27.99602349804947	23.33694788681763
32	23.65929899359588	25.86205621247065	28.44752215701561	22.031122636917853
33	22.492237093011386	24.441433138403866	30.513439186000586	22.552890582584165
34	21.898572571900274	23.175698365758294	30.498497716618022	24.42723134572341
35	22.502444631500467	22.722276547366675	29.658520853705262	25.116757967427596
36	23.391683962773552	24.252371773345306	30.63489410048656	21.721050163394583
37	21.987925517514807	24.804318528457582	31.948116117407405	21.25963983662021
38	22.294595478208386	24.615257163399022	30.542878318744442	22.547269039648153
39	20.658430613147605	23.302922758520705	32.92005130397605	23.11859532435563
40	22.892106318170455	22.02505728796058	31.51747634144068	23.565360052428286
41	20.973532888245206	23.40632956147527	29.628194108918876	25.99194344136065
42	23.143300526206005	22.690470449176072	29.83308455540252	24.333144469215394
43	22.738253564132187	22.70703920730327	31.8720773524308	22.68262987613374
44	21.918839713538006	23.491984123579265	30.05735453148138	24.531821631401346
45	22.63854514468816	22.369006954440355	28.784518862495577	26.207929038375905
46	22.916071843318722	22.80867078617278	30.74540179978135	23.529855570727147
47	20.563899930618327	23.305881465329133	32.77329944627802	23.35691915777452
48	21.42858199538146	21.046021205051694	33.539900380341756	23.985496419225086
49	21.738210662883468	21.18404487766487	33.741684184676565	23.3360602747751
50	21.554031164058813	20.956520324096743	31.506824996930344	25.9826235149141
51	20.269804473860564	21.02102013252048	29.412356447244036	29.29681894637492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	539.5
2	961.0
3	3296.5
4	5632.0
5	3796.0
6	1960.0
7	1881.0
8	1802.0
9	1903.5
10	2005.0
11	2046.5
12	2088.0
13	2014.5
14	1941.0
15	1881.5
16	1822.0
17	1752.5
18	1683.0
19	1722.5
20	1762.0
21	1939.0
22	2116.0
23	2490.0
24	2864.0
25	3289.0
26	4836.5
27	5959.0
28	6739.5
29	7520.0
30	8937.5
31	10355.0
32	11673.0
33	12991.0
34	14759.0
35	16527.0
36	18611.5
37	20696.0
38	23149.5
39	25603.0
40	29758.0
41	33913.0
42	37438.0
43	40963.0
44	45162.0
45	49361.0
46	55932.0
47	62503.0
48	70093.5
49	77684.0
50	74313.5
51	70943.0
52	59864.0
53	48785.0
54	42400.0
55	36015.0
56	32639.5
57	29264.0
58	26975.5
59	24687.0
60	23555.5
61	22424.0
62	19524.5
63	16625.0
64	13396.5
65	10168.0
66	8910.5
67	7653.0
68	6326.0
69	4999.0
70	4360.5
71	3722.0
72	3172.0
73	2622.0
74	2131.0
75	1295.0
76	950.0
77	722.5
78	495.0
79	385.0
80	275.0
81	182.0
82	89.0
83	68.5
84	48.0
85	29.0
86	10.0
87	9.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	675971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.83614333727801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.75588850512814	25.356181624262337
2	11.509741474196217	8.249294904886183
3	4.37846220751451	4.70721597796034
4	2.312765472473716	3.3152237990830224
5	1.5368035394268174	2.7536555960067806
6	1.2048602649972806	2.590652709469999
7	0.9433332108245903	2.3663796910547297
8	0.8017461950517805	2.2985193252794285
9	0.6614348161258012	2.133294559105531
>10	5.758698583272037	38.78628314195931
>50	0.09013851155508436	2.210044450005754
>100	0.04104899344131867	2.5417103495632984
>500	0.002539112996370227	0.6452850559303004
>1k	0.002539112996370227	2.0462588154330303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	3642	0.5387805098147702	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	2747	0.40637838013760946	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2478	0.36658377356425054	No Hit
TCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1821	0.2693902549073851	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1801	0.2664315480989569	No Hit
CCTGTATCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	1004	0.14852708178309423	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	873	0.1291475521878897	No Hit
CTGTCACTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	808	0.11953175506049815	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	771	0.11405814746490604	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTG	750	0.11095150531605646	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4793534042140862E-4	0.0	0.0	0.43241500005177735	0.0
2	1.4793534042140862E-4	0.0	0.0	1.6087968270828186	0.0
3	1.4793534042140862E-4	0.0	0.0	2.2190301063211293	0.0
4	1.4793534042140862E-4	0.0	0.0	2.8390271180272526	0.0
5	1.4793534042140862E-4	0.0	0.0	3.8751662423387985	0.0
6	1.4793534042140862E-4	0.0	0.0	4.532147089150274	0.0
7	1.4793534042140862E-4	0.0	0.0	5.317387876107111	0.0
8	1.4793534042140862E-4	0.0	0.0	6.555014934072616	0.0
9	1.4793534042140862E-4	0.0	0.0	7.1082931072486835	0.0
10	1.4793534042140862E-4	0.0	0.0	8.227867763557905	0.0
11	1.4793534042140862E-4	0.0	0.0	10.30473200773406	0.0
12	1.4793534042140862E-4	0.0	0.0	12.13853848759784	0.0
13	1.4793534042140862E-4	0.0	0.0	12.719480569432712	0.0
14	1.4793534042140862E-4	0.0	0.0	12.931176041575748	0.0
15	1.4793534042140862E-4	0.0	0.0	13.264326428204761	0.0
16	1.4793534042140862E-4	0.0	0.0	14.075751770416186	0.0
17	1.4793534042140862E-4	0.0	0.0	15.138223385322743	0.0
18	1.4793534042140862E-4	0.0	0.0	16.342860862374273	0.0
19	1.4793534042140862E-4	0.0	0.0	17.11863378754414	0.0
20	1.4793534042140862E-4	0.0	0.0	17.941450150968016	0.0
21	1.4793534042140862E-4	0.0	0.0	19.114281529828943	0.0
22	1.4793534042140862E-4	0.0	0.0	20.2797161416688	0.0
23	1.4793534042140862E-4	0.0	0.0	21.369555794553317	0.0
24	1.4793534042140862E-4	0.0	0.0	22.25539261299671	0.0
25	1.4793534042140862E-4	0.0	0.0	23.024656383188034	0.0
26	1.4793534042140862E-4	0.0	0.0	23.693324121892804	0.0
27	1.4793534042140862E-4	0.0	0.0	24.36095631321462	0.0
28	1.4793534042140862E-4	0.0	0.0	25.048559775493327	0.0
29	1.4793534042140862E-4	0.0	0.0	25.78113558126014	0.0
30	1.4793534042140862E-4	0.0	0.0	26.594484082897047	0.0
31	1.4793534042140862E-4	0.0	0.0	27.33194175489777	0.0
32	1.4793534042140862E-4	0.0	0.0	28.062298530558262	0.0
33	1.4793534042140862E-4	0.0	0.0	28.757446695198464	0.0
34	1.4793534042140862E-4	0.0	0.0	29.44904441166855	0.0
35	1.4793534042140862E-4	0.0	0.0	30.1749631271164	0.0
36	2.9587068084281724E-4	0.0	0.0	30.846441637289175	0.0
37	2.9587068084281724E-4	0.0	0.0	31.536707935695468	0.0
38	2.9587068084281724E-4	0.0	0.0	32.25389846605845	0.0
39	2.9587068084281724E-4	0.0	0.0	32.95599959169846	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAT	25	3.8890947E-5	45.0	21
TCGTTGA	50	2.1827873E-11	45.0	24
ACGCATA	20	7.0312223E-4	45.0	34
ACTTCGC	20	7.0312223E-4	45.0	31
ACGGGTA	20	7.0312223E-4	45.0	5
AGACGGT	25	3.8890947E-5	45.0	17
CGTGTAG	25	3.8890947E-5	45.0	20
CGTAAGC	20	7.0312223E-4	45.0	39
TGTCGAA	20	7.0312223E-4	45.0	40
GTTACGT	30	2.16417E-6	44.999996	31
CCGTAAC	30	2.16417E-6	44.999996	13
ATAAGCG	30	2.16417E-6	44.999996	33
TCCGTAA	30	2.16417E-6	44.999996	12
TTACGTA	30	2.16417E-6	44.999996	32
TAGGGAC	500	0.0	43.649998	5
TTAGGGA	1300	0.0	42.923077	4
CGCTAGG	90	0.0	42.5	2
CGTTTAT	460	0.0	42.06522	1
AGGGCGA	355	0.0	41.830986	6
CATTCTA	70	0.0	41.785713	45
>>END_MODULE