FastQCFastQC Report
Sat 14 Jan 2017
SRR2934314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688786
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260361.541699185095092No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC110040.6515923272694113No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC91590.542342250587108No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG86500.5122022565322072No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT70110.41515029139275195No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT57780.3421392645367738No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC56530.33473749782388057No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG54750.3241973820247207TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT43650.2584696936142294TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC40420.23934352842811343TruSeq Adapter, Index 19 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC39230.23229704651743913No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC36910.21855936749830943TruSeq Adapter, Index 19 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT31610.18717587663564242No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC29490.1746224802905756No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT29200.17290527041318438No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT27470.16266122528254023No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC25270.14963411586784828No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC23110.13684386298796888No Hit
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC22860.13536350964539023TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT19330.11446092044817993No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA207.034227E-445.00000416
CGTTTAT59350.044.6208951
CGTTTTT149350.044.0810171
CGTTAGG3250.043.6153872
CGTTATT6600.042.6136361
GTTTATT61800.042.1237872
TAGGGAT11950.041.0460245
GCGTTAG1100.040.9090921
GTTAGGG18000.040.6253
TTAGGGA27050.040.5914964
TACGCGG501.0822987E-940.52
CGTAAGG501.0822987E-940.52
CGTTTTC7100.040.2464751
AGGGCGA7950.040.188686
TAGGGCG7000.040.178575
CTTAGGG26300.040.038023
TCTTGCG3050.039.8360631
CTAGGGA14500.039.724144
TTCGTAG2950.039.661021
TCTTAGG15350.039.5765462
Produced by FastQC (version 0.11.5)