##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688786
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21127958190085	33.0	31.0	34.0	30.0	34.0
2	32.42236138859512	34.0	31.0	34.0	31.0	34.0
3	32.311731622597534	34.0	31.0	34.0	30.0	34.0
4	35.971606230748	37.0	35.0	37.0	35.0	37.0
5	35.12181709227812	37.0	35.0	37.0	35.0	37.0
6	25.972478454937452	36.0	0.0	37.0	0.0	37.0
7	31.02210345182871	37.0	19.0	37.0	17.0	37.0
8	34.64248696992988	37.0	32.0	37.0	32.0	37.0
9	37.22114998584782	39.0	37.0	39.0	34.0	39.0
10	37.39230370218607	39.0	37.0	39.0	34.0	39.0
11	37.46229421608185	39.0	37.0	39.0	35.0	39.0
12	37.29334030481068	39.0	37.0	39.0	34.0	39.0
13	37.33272480941931	39.0	37.0	39.0	34.0	39.0
14	38.50934931957039	40.0	38.0	41.0	34.0	41.0
15	38.5916688082445	40.0	38.0	41.0	34.0	41.0
16	38.67477169990751	40.0	38.0	41.0	35.0	41.0
17	38.62600590009628	40.0	38.0	41.0	34.0	41.0
18	38.464152355597456	40.0	38.0	41.0	34.0	41.0
19	38.342408096703785	40.0	37.0	41.0	34.0	41.0
20	38.121545299404424	40.0	36.0	41.0	34.0	41.0
21	37.99745379225077	39.0	36.0	41.0	34.0	41.0
22	37.96408011435434	39.0	36.0	41.0	34.0	41.0
23	37.855104791252415	39.0	35.0	41.0	34.0	41.0
24	37.78068150730762	39.0	35.0	41.0	34.0	41.0
25	37.66241015735564	39.0	35.0	41.0	33.0	41.0
26	37.60985939011811	39.0	35.0	41.0	33.0	41.0
27	37.56164842673968	39.0	35.0	41.0	33.0	41.0
28	37.46050358067866	39.0	35.0	41.0	33.0	41.0
29	37.406268763478614	39.0	35.0	41.0	33.0	41.0
30	37.06056066310355	39.0	35.0	41.0	33.0	41.0
31	36.94389164760958	39.0	35.0	41.0	32.0	41.0
32	36.61651979587704	39.0	35.0	41.0	31.0	41.0
33	36.14165501135135	39.0	35.0	41.0	30.0	41.0
34	35.76755314172429	39.0	35.0	41.0	26.0	41.0
35	35.55293743553061	39.0	35.0	41.0	24.0	41.0
36	35.40842652651076	39.0	35.0	41.0	23.0	41.0
37	35.32620947828795	39.0	35.0	41.0	23.0	41.0
38	35.2111605614921	39.0	35.0	41.0	23.0	41.0
39	35.13144353399424	39.0	35.0	41.0	22.0	41.0
40	35.00835096927616	39.0	35.0	41.0	21.0	41.0
41	34.888725392086386	39.0	35.0	40.0	21.0	41.0
42	34.83998505435265	39.0	35.0	40.0	20.0	41.0
43	34.77204157305899	39.0	35.0	40.0	20.0	41.0
44	34.651712531960825	38.0	35.0	40.0	18.0	41.0
45	34.63685511367337	38.0	35.0	40.0	20.0	41.0
46	34.56693151174868	38.0	34.0	40.0	20.0	41.0
47	34.46414880274943	38.0	34.0	40.0	20.0	41.0
48	34.408408762270646	38.0	34.0	40.0	19.0	41.0
49	34.40927151219871	38.0	34.0	40.0	18.0	41.0
50	34.30633958358253	38.0	34.0	40.0	18.0	41.0
51	33.397146234040314	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	9.0
11	9.0
12	5.0
13	17.0
14	12.0
15	43.0
16	89.0
17	207.0
18	403.0
19	867.0
20	1637.0
21	3024.0
22	4980.0
23	8678.0
24	16064.0
25	27080.0
26	36854.0
27	37735.0
28	33448.0
29	30708.0
30	31136.0
31	36442.0
32	46710.0
33	67503.0
34	97499.0
35	123703.0
36	151781.0
37	244453.0
38	340043.0
39	347450.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.79785597464688	3.6310698928105754	47.3625432707282	26.208530861814346
2	29.844278671187467	6.882340332049177	43.6970699662361	19.576311030527254
3	16.62940123852282	7.014091779538674	55.651752205430405	20.704754776508093
4	15.628800807207071	3.7095878341009456	58.1421802407173	22.519431117974687
5	17.503757136783467	7.0082295803020624	53.241204036509075	22.246809246405405
6	13.856344143070821	31.719531071432378	42.21878911833708	12.205335667159723
7	70.59799169344132	1.514815968393864	22.638155456049493	5.249036882115318
8	71.07916574391308	3.2997076006077735	20.26165541400746	5.3594712414716845
9	65.90912051615776	3.4929233188811373	22.018894045782	8.579062119179103
10	33.57713765983375	20.603143323073496	29.482006601191625	16.337712415901127
11	23.733794571958793	21.13695873840735	35.97157958438784	19.157667105246016
12	21.25888063970213	18.633089094769854	39.51045307102262	20.5975771945054
13	20.622328702393318	18.64072771801756	42.69220611729372	18.0447374622954
14	17.28809926183661	22.07781210881663	41.39328488038153	19.24080374896523
15	15.566922037487283	22.763334134697942	43.696004111829446	17.973739715985328
16	18.473566218573577	23.291464993196296	38.80142303406116	19.43354575416897
17	18.238959820841714	24.132009621112445	37.57083490744238	20.05819565060345
18	18.168021288665347	23.68991689888476	38.13526402990077	20.00679778254912
19	18.2838441341887	25.853186845461767	35.931906114806736	19.9310629055428
20	18.681644684406432	26.84816193407572	36.17752634140738	18.29266704011047
21	19.22357243605762	26.375870003659436	36.91296588200044	17.487591678282506
22	17.995352874786978	22.49071226312866	37.77299195990493	21.740942902179437
23	17.461596673586826	24.1254960664051	37.23136027892225	21.181546981085823
24	18.71604809608796	23.24486346997192	36.47205744244682	21.567030991493297
25	18.98274855428693	25.718593119554523	34.276989506071224	21.02166882008733
26	17.810604777633163	24.043602919493647	35.73507833437748	22.410713968495713
27	18.625983398725477	23.10061784027106	36.56810276731333	21.70529599369014
28	16.882482445970066	22.289561850939076	37.62922004327368	23.19873565981717
29	17.938684948833068	21.185573542177636	38.069595555623984	22.806145953365316
30	18.52614836930197	21.79980175108036	37.57515753920272	22.09889234041495
31	17.59838132244109	22.053652742265744	37.28340950244732	23.064556432845844
32	17.84950846347613	22.948615159055088	37.079002312904066	22.122874064564723
33	17.454194906873933	23.20791385054116	34.81891725772241	24.518973984862498
34	16.01831137870636	23.41279475315404	35.95091385172544	24.61798001641416
35	15.522570651343628	23.462416197197278	33.744713658213655	27.270299493245442
36	16.51588774421389	23.84559085639033	32.72978340654174	26.908737992854036
37	15.800936293882115	23.79679841021894	34.09727461028218	26.30499068561677
38	16.698740989089202	24.15717562793628	33.619179694762984	25.52490368821153
39	17.97977955762305	23.55567845777973	32.5577663481341	25.906775636463113
40	18.847740329443756	23.340612724169908	32.79379388507484	25.017853061311495
41	17.447681352166587	23.702055796293905	30.992085438889237	27.85817741265027
42	17.886517297040598	23.16563495907711	33.09519382562385	25.85265391825844
43	18.610350867427844	22.736569346264123	33.56049848826317	25.09258129804487
44	19.774382307764277	22.55880851688728	32.30107307853097	25.365736096817475
45	18.86289914767176	21.533456577683612	32.056104207401056	27.54754006724357
46	20.00093558331251	22.65763690603783	32.125325529700035	25.216101980949627
47	17.39723091025151	22.15271798795111	35.47613492769362	24.973916174103763
48	17.351695241433788	22.533938580731956	34.01917116792773	26.095195009906526
49	18.405292322413853	20.86445529510548	35.31548698295699	25.41476539952368
50	17.479242485430362	20.770304822517478	34.89761284141389	26.852839850638272
51	16.724380708982668	20.68870774627454	33.15873059108733	29.428180953655463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	276.0
1	895.5
2	1515.0
3	29153.5
4	56792.0
5	37468.0
6	18144.0
7	17973.5
8	17803.0
9	17993.0
10	18183.0
11	18208.5
12	18234.0
13	17595.5
14	16957.0
15	16197.5
16	15438.0
17	14829.5
18	14221.0
19	13425.0
20	12629.0
21	12326.0
22	12023.0
23	11977.5
24	11932.0
25	12559.0
26	14081.0
27	14976.0
28	16439.5
29	17903.0
30	20259.0
31	22615.0
32	26132.5
33	29650.0
34	33646.5
35	37643.0
36	41365.5
37	45088.0
38	49657.5
39	54227.0
40	60238.5
41	66250.0
42	75626.5
43	85003.0
44	100894.0
45	116785.0
46	163696.5
47	210608.0
48	202329.0
49	194050.0
50	182041.0
51	170032.0
52	140120.0
53	110208.0
54	92747.0
55	75286.0
56	66001.0
57	56716.0
58	51380.5
59	46045.0
60	40453.5
61	34862.0
62	29839.0
63	24816.0
64	21292.0
65	17768.0
66	14950.5
67	12133.0
68	9609.5
69	7086.0
70	6038.5
71	4991.0
72	3949.0
73	2907.0
74	2326.5
75	1344.0
76	942.0
77	748.5
78	555.0
79	441.5
80	328.0
81	220.0
82	112.0
83	78.0
84	44.0
85	38.5
86	33.0
87	30.0
88	27.0
89	17.5
90	8.0
91	7.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1688786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.91999996033642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47744763946436	32.82412049652441
2	9.522937881951133	8.174489870312577
3	3.55479864094402	4.577158725849639
4	1.9454123224837996	3.339883872153706
5	1.3683519807306603	2.9364833479343098
6	1.0663112819056582	2.745964810625828
7	0.8943491403066023	2.6869825546540396
8	0.7682091595133412	2.6377229676674143
9	0.666365660048721	2.574037269257459
>10	3.6118677475057726	23.325706366154037
>50	0.07662032414145103	2.2419257433214024
>100	0.04166112763949891	3.2841687213794275
>500	0.0015316468554119925	0.45517187130873643
>1k	0.002910129025282786	3.01824178616378
>5k	9.189881132471956E-4	2.742995595353601
>10k+	3.063293710823985E-4	2.43494600133963
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26036	1.541699185095092	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	11004	0.6515923272694113	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	9159	0.542342250587108	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	8650	0.5122022565322072	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	7011	0.41515029139275195	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	5778	0.3421392645367738	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	5653	0.33473749782388057	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	5475	0.3241973820247207	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	4365	0.2584696936142294	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	4042	0.23934352842811343	TruSeq Adapter, Index 19 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	3923	0.23229704651743913	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	3691	0.21855936749830943	TruSeq Adapter, Index 19 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3161	0.18717587663564242	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	2949	0.1746224802905756	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2920	0.17290527041318438	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	2747	0.16266122528254023	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	2527	0.14963411586784828	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	2311	0.13684386298796888	No Hit
TCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	2286	0.13536350964539023	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGT	1933	0.11446092044817993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7764240110943602E-4	0.0	0.0	0.275404935853329	0.0
2	1.7764240110943602E-4	0.0	0.0	0.9168124321257992	0.0
3	1.7764240110943602E-4	0.0	0.0	1.5235796601819296	0.0
4	1.7764240110943602E-4	0.0	0.0	2.3204834715588594	0.0
5	1.7764240110943602E-4	0.0	0.0	4.241449183022597	0.0
6	1.7764240110943602E-4	0.0	0.0	6.064237860806521	0.0
7	1.7764240110943602E-4	0.0	0.0	8.049273264937062	0.0
8	1.7764240110943602E-4	0.0	0.0	10.273770625763122	0.0
9	1.7764240110943602E-4	0.0	0.0	11.590337674518855	0.0
10	2.3685653481258134E-4	0.0	0.0	13.679589953966932	0.0
11	2.3685653481258134E-4	0.0	0.0	15.773460935843854	0.0
12	2.9607066851572667E-4	0.0	0.0	17.93951394670491	0.0
13	3.5528480221887204E-4	0.0	0.0	18.694908650355938	0.0
14	3.5528480221887204E-4	0.0	0.0	19.10893387320833	0.0
15	3.5528480221887204E-4	0.0	0.0	19.53557170653949	0.0
16	4.1449893592201736E-4	0.0	0.0	20.123212769409506	0.0
17	4.1449893592201736E-4	0.0	0.0	20.883759102692704	0.0
18	4.1449893592201736E-4	0.0	0.0	21.67095179614232	0.0
19	4.1449893592201736E-4	0.0	0.0	22.37417884800087	0.0
20	5.329272033283081E-4	0.0	0.0	23.02950166569358	0.0
21	5.329272033283081E-4	0.0	0.0	23.863355096501273	0.0
22	5.329272033283081E-4	0.0	0.0	24.81380115657046	0.0
23	5.329272033283081E-4	0.0	0.0	25.70337508719281	0.0
24	5.329272033283081E-4	0.0	0.0	26.40589156944693	0.0
25	5.329272033283081E-4	0.0	0.0	27.020238206617062	0.0
26	5.921413370314533E-4	0.0	0.0	27.56429766708156	0.0
27	5.921413370314533E-4	0.0	0.0	28.141339400018712	0.0
28	5.921413370314533E-4	0.0	0.0	28.699195753636044	0.0
29	5.921413370314533E-4	0.0	0.0	29.330299990644168	0.0
30	5.921413370314533E-4	0.0	0.0	30.024052781110218	0.0
31	5.921413370314533E-4	0.0	0.0	30.621523390174954	0.0
32	5.921413370314533E-4	0.0	0.0	31.19897962204803	0.0
33	5.921413370314533E-4	0.0	0.0	31.77158029495744	0.0
34	5.921413370314533E-4	0.0	0.0	32.3599911415656	0.0
35	6.513554707345987E-4	0.0	0.0	32.96752815335987	0.0
36	6.513554707345987E-4	0.0	0.0	33.53687204891561	0.0
37	6.513554707345987E-4	0.0	0.0	34.112847927446104	0.0
38	7.105696044377441E-4	0.0	0.0	34.72790513422068	0.0
39	7.105696044377441E-4	0.0	0.0	35.55850178767469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	20	7.034227E-4	45.000004	16
CGTTTAT	5935	0.0	44.620895	1
CGTTTTT	14935	0.0	44.081017	1
CGTTAGG	325	0.0	43.615387	2
CGTTATT	660	0.0	42.613636	1
GTTTATT	6180	0.0	42.123787	2
TAGGGAT	1195	0.0	41.046024	5
GCGTTAG	110	0.0	40.909092	1
GTTAGGG	1800	0.0	40.625	3
TTAGGGA	2705	0.0	40.591496	4
TACGCGG	50	1.0822987E-9	40.5	2
CGTAAGG	50	1.0822987E-9	40.5	2
CGTTTTC	710	0.0	40.246475	1
AGGGCGA	795	0.0	40.18868	6
TAGGGCG	700	0.0	40.17857	5
CTTAGGG	2630	0.0	40.03802	3
TCTTGCG	305	0.0	39.836063	1
CTAGGGA	1450	0.0	39.72414	4
TTCGTAG	295	0.0	39.66102	1
TCTTAGG	1535	0.0	39.576546	2
>>END_MODULE