FastQCFastQC Report
Sat 14 Jan 2017
SRR2934313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450691
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC29730.20493681976382289TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.19942220638302713No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT28090.19363186233319157TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC26480.18253370290434007TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC22790.15709754868541956TruSeq Adapter, Index 19 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.033899E-445.08
TACGCGG351.2122291E-745.02
TTTAGGG52300.041.0850833
TTAGGGT10500.040.928574
CGTTAGG4350.040.8620722
CTTAGGG25750.040.368933
TTAGGGA26850.040.3072624
TAGGGTA5900.040.0423745
TCGTTAG1800.040.01
TGTTTAG7150.039.650351
TTTTAGG29750.039.6302532
TAGGGTG5250.039.428575
AGGGCGA10850.039.400926
TAGGGAT13950.039.354845
TAGGGCG8700.039.3103455
CTAGGGG25200.039.196434
GTTTAGG10050.039.1791042
GCTAGGG16800.039.1071433
TTGCGAG1850.038.918921
CTAGGGT5550.038.918924
Produced by FastQC (version 0.11.5)