##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934313.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1450691
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40294521714135	33.0	31.0	34.0	31.0	34.0
2	32.574326993136374	34.0	31.0	34.0	31.0	34.0
3	32.63283704110662	34.0	31.0	34.0	31.0	34.0
4	36.07748652194024	37.0	35.0	37.0	35.0	37.0
5	35.1637143954157	37.0	35.0	37.0	35.0	37.0
6	25.951998047826862	37.0	0.0	37.0	0.0	37.0
7	31.037875054025978	37.0	19.0	37.0	17.0	37.0
8	34.759759314699	37.0	32.0	37.0	32.0	37.0
9	37.28277351965374	39.0	37.0	39.0	35.0	39.0
10	37.39598577505478	39.0	37.0	39.0	35.0	39.0
11	37.43818635395132	39.0	37.0	39.0	35.0	39.0
12	37.17945654863786	39.0	37.0	39.0	34.0	39.0
13	37.196080350674265	39.0	37.0	39.0	34.0	39.0
14	38.25445804792337	40.0	38.0	41.0	33.0	41.0
15	38.42791538652959	40.0	38.0	41.0	34.0	41.0
16	38.53896108819866	40.0	38.0	41.0	34.0	41.0
17	38.529040298726606	40.0	38.0	41.0	34.0	41.0
18	38.51272945099956	40.0	38.0	41.0	35.0	41.0
19	38.5081171662332	40.0	37.0	41.0	34.0	41.0
20	38.43970976589777	40.0	37.0	41.0	34.0	41.0
21	38.3375949805989	40.0	37.0	41.0	34.0	41.0
22	38.352655389741855	40.0	37.0	41.0	34.0	41.0
23	38.294705764356436	40.0	37.0	41.0	34.0	41.0
24	38.244974291561746	40.0	37.0	41.0	34.0	41.0
25	38.12858492952669	40.0	37.0	41.0	34.0	41.0
26	38.09232358924127	40.0	37.0	41.0	34.0	41.0
27	38.07583903119272	40.0	37.0	41.0	34.0	41.0
28	37.990675478099746	40.0	36.0	41.0	34.0	41.0
29	37.96690060116179	40.0	36.0	41.0	34.0	41.0
30	37.741014454491	40.0	36.0	41.0	33.0	41.0
31	37.74274190713253	40.0	36.0	41.0	33.0	41.0
32	37.69525763929052	40.0	36.0	41.0	33.0	41.0
33	37.61040359387354	40.0	36.0	41.0	33.0	41.0
34	37.52321755632316	40.0	36.0	41.0	33.0	41.0
35	37.49528121426272	40.0	36.0	41.0	33.0	41.0
36	37.412263535101545	40.0	35.0	41.0	33.0	41.0
37	37.347800461986736	40.0	35.0	41.0	33.0	41.0
38	37.24963827582855	40.0	35.0	41.0	33.0	41.0
39	37.21354788855793	39.0	35.0	41.0	33.0	41.0
40	37.12594342971729	39.0	35.0	41.0	32.0	41.0
41	37.05771525431673	39.0	35.0	41.0	32.0	41.0
42	37.01241822000688	39.0	35.0	41.0	32.0	41.0
43	36.92290708358982	39.0	35.0	41.0	32.0	41.0
44	36.788712413601516	39.0	35.0	41.0	31.0	41.0
45	36.74079731658913	39.0	35.0	41.0	32.0	41.0
46	36.67490802658871	39.0	35.0	41.0	31.0	41.0
47	36.608672694598646	39.0	35.0	41.0	31.0	41.0
48	36.55825740974473	39.0	35.0	41.0	31.0	41.0
49	36.52333060589746	39.0	35.0	40.0	31.0	41.0
50	36.43434956169163	39.0	35.0	40.0	31.0	41.0
51	35.441218012657416	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	19.0
10	31.0
11	28.0
12	13.0
13	14.0
14	28.0
15	44.0
16	75.0
17	151.0
18	250.0
19	485.0
20	799.0
21	1360.0
22	2079.0
23	3133.0
24	4680.0
25	7046.0
26	9045.0
27	10694.0
28	12485.0
29	14860.0
30	19231.0
31	25933.0
32	36966.0
33	61265.0
34	93040.0
35	114379.0
36	135692.0
37	228637.0
38	319790.0
39	348241.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.953631752040923	4.375845717661445	52.91250859073365	16.758013939563973
2	21.069890142008187	4.310359683764496	51.876174871147605	22.743575303079705
3	22.467293172701837	4.789303855886608	50.408460519848816	22.33494245156274
4	19.93291472822262	5.202210532773692	49.088468874488086	25.7764058645156
5	18.658694373922497	8.596041472649931	48.079501423804246	24.66576272962333
6	16.72340974059948	31.864814767583173	38.069168416981974	13.342607074835373
7	84.7192131198167	2.3823129805037735	8.75258755999727	4.145886339682262
8	85.86342646366457	2.684169130435082	7.206496766023915	4.245907639876445
9	80.49281342477481	4.702862291142635	9.471624212185779	5.332700071896772
10	41.42570678387058	24.90289110499755	17.43837936541965	16.233022745712216
11	30.6588377538704	25.062814893040624	24.754065476383325	19.52428187670565
12	27.243361956474537	22.84276941126677	29.339604367849525	20.574264264409166
13	25.737114244177427	22.771079437316423	31.362364555925414	20.12944176258073
14	21.892877256424697	24.336953906793383	32.84751887204098	20.92264996474094
15	20.132474799940166	23.68960722855522	34.46805694665508	21.709861024849538
16	23.990360455810368	23.90874417777459	30.81448771654336	21.286407649871684
17	23.995806136523907	23.63494362341808	28.945860972460707	23.4233892675973
18	25.160837145884273	22.366582545835055	30.78932729299348	21.6832530152872
19	24.897376491616754	24.780742418612924	28.427142651329607	21.894738438440715
20	25.817489734202525	26.98031489821058	27.879403677282067	19.32279169030483
21	25.568711738061378	25.929849981836245	29.29528066280138	19.206157617301
22	24.2358986165903	22.34486875564817	30.48760900839669	22.931623619364842
23	22.785210634104715	24.598208715708584	29.355872477322876	23.26070817286383
24	22.562971714858644	25.114652258820108	29.839710868820447	22.482665157500804
25	23.237546796664486	25.92867812649282	27.317602439113497	23.516172637729195
26	21.78596268950452	25.782885535238037	28.05063242275578	24.38051935250167
27	21.741501119121853	25.1988190455445	30.19816073857217	22.861519096761473
28	19.278123321920383	25.93350341320102	30.09710544836909	24.691267816509512
29	20.732120072434444	25.228391159799017	29.64835378450683	24.3911349832597
30	23.94893192278714	24.465926927236744	29.447552924778602	22.13758822519751
31	23.294485179821205	26.61469603106382	27.76773275632095	22.323086032794027
32	23.70380735801077	25.429123086859985	27.622974155075063	23.24409540005418
33	24.221767419802013	24.842712886479614	26.760281824316827	24.17523786940155
34	20.865780514251483	24.904683354346307	29.806485323201148	24.423050808201058
35	21.20189619981099	25.4170598700895	29.102958521146128	24.278085408953388
36	24.05267558701336	26.83383298028319	27.125418162792762	21.988073269910686
37	22.113875387660087	27.27755255943547	27.062000108913615	23.54657194399083
38	21.936098038796683	27.253426125894485	27.17525648122171	23.635219354087123
39	21.71737468558087	25.1991637088808	27.06613606894921	26.017325536589116
40	23.174680204123415	24.33895295414392	28.532402834235548	23.953964007497117
41	21.39332221679186	23.91060535979061	28.34690502663903	26.3491673967785
42	23.02619923884549	23.40270946741932	29.76581504951778	23.80527624421741
43	23.017169059434437	23.346184680266163	29.27087849859136	24.36576776170804
44	22.683948545899852	24.555470462007417	27.64337822458401	25.117202767508722
45	22.219135570566024	23.263603344888747	26.991688788308466	27.52557229623676
46	22.57758544031775	23.646662176852274	28.5674206292036	25.20833175362637
47	19.99329974474233	25.628062764572196	30.66511062659105	23.713526864094423
48	20.95277354033354	25.59525081495646	29.33967330051679	24.112302344193214
49	23.159652882660744	23.486393725472894	29.475401722351624	23.878551669514735
50	21.30033204865819	24.00676643061824	28.318297969726153	26.37460355099742
51	20.132681597941946	23.57083624286633	27.397495400467776	28.898986758723943
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	227.0
1	883.5
2	1540.0
3	4249.5
4	6959.0
5	4702.5
6	2446.0
7	2463.0
8	2480.0
9	2554.0
10	2628.0
11	2729.5
12	2831.0
13	2818.0
14	2805.0
15	2828.0
16	2851.0
17	2956.5
18	3062.0
19	3416.5
20	3771.0
21	4216.0
22	4661.0
23	5150.5
24	5640.0
25	6815.5
26	9672.5
27	11354.0
28	13302.5
29	15251.0
30	18622.5
31	21994.0
32	25026.0
33	28058.0
34	32851.0
35	37644.0
36	40342.5
37	43041.0
38	47801.0
39	52561.0
40	58970.0
41	65379.0
42	72593.5
43	79808.0
44	87914.5
45	96021.0
46	107978.5
47	119936.0
48	135134.0
49	150332.0
50	147897.0
51	145462.0
52	130960.5
53	116459.0
54	105674.0
55	94889.0
56	86501.5
57	78114.0
58	71760.5
59	65407.0
60	58510.0
61	51613.0
62	45757.5
63	39902.0
64	34353.5
65	28805.0
66	24219.0
67	19633.0
68	16766.0
69	13899.0
70	11725.5
71	9552.0
72	7996.5
73	6441.0
74	5320.5
75	3425.0
76	2650.0
77	2016.5
78	1383.0
79	1002.0
80	621.0
81	441.5
82	262.0
83	165.0
84	68.0
85	52.5
86	37.0
87	27.0
88	17.0
89	11.0
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1450691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.882719875925286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.02559824851585	22.644892529774403
2	11.078085907343072	7.063990194905095
3	4.192961251278674	4.010490270753814
4	2.1036032115985654	2.682743677019754
5	1.3740181070851958	2.1903717206323203
6	0.9735024165934926	1.8622742908071988
7	0.7687724699030549	1.7157390114369613
8	0.6832926553430065	1.742818265886259
9	0.6051515925779363	1.7364490837769169
>10	6.982665136037553	46.63886283909199
>50	0.16164859813655374	3.345284143422073
>100	0.04607023210900067	2.5332765260450163
>500	0.0032411214346425494	0.7491843091880999
>1k	0.0013890520434182356	1.083623137260147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2973	0.20493681976382289	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2893	0.19942220638302713	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2809	0.19363186233319157	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2648	0.18253370290434007	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2279	0.15709754868541956	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20355816641862395	0.0
2	0.0	0.0	0.0	0.7932771348274719	0.0
3	0.0	0.0	0.0	1.1360103564439292	0.0
4	0.0	0.0	0.0	1.5307188091743866	0.0
5	0.0	0.0	0.0	2.239967022611983	0.0
6	0.0	0.0	0.0	2.8067314128232685	0.0
7	0.0	0.0	0.0	3.4851667239956683	0.0
8	0.0	0.0	0.0	4.546523001797075	0.0
9	0.0	0.0	0.0	5.041459552723495	0.0
10	0.0	0.0	0.0	5.9717058973964825	0.0
11	0.0	0.0	0.0	7.6968148282439195	0.0
12	0.0	0.0	0.0	9.205406251227863	0.0
13	0.0	0.0	0.0	9.721367265668567	0.0
14	6.893266725994716E-5	0.0	0.0	9.911414629304241	0.0
15	6.893266725994716E-5	0.0	0.0	10.194107497737285	0.0
16	6.893266725994716E-5	0.0	0.0	10.806160650338356	0.0
17	6.893266725994716E-5	0.0	0.0	11.60805436857332	0.0
18	6.893266725994716E-5	0.0	0.0	12.485774020794228	0.0
19	6.893266725994716E-5	0.0	0.0	13.113405956196047	0.0
20	6.893266725994716E-5	0.0	0.0	13.778537262587278	0.0
21	6.893266725994716E-5	0.0	0.0	14.71574580665352	0.0
22	6.893266725994716E-5	0.0	0.0	15.697691651771466	0.0
23	6.893266725994716E-5	0.0	0.0	16.628558390449793	0.0
24	6.893266725994716E-5	0.0	0.0	17.363518488775348	0.0
25	6.893266725994716E-5	0.0	0.0	17.986118339467193	0.0
26	6.893266725994716E-5	0.0	0.0	18.53930299422827	0.0
27	6.893266725994716E-5	0.0	0.0	19.13432977801613	0.0
28	6.893266725994716E-5	0.0	0.0	19.70998648230395	0.0
29	6.893266725994716E-5	0.0	0.0	20.38855965881087	0.0
30	6.893266725994716E-5	0.0	0.0	21.172117287554688	0.0
31	6.893266725994716E-5	0.0	0.0	21.804367711662923	0.0
32	6.893266725994716E-5	0.0	0.0	22.405460570169662	0.0
33	6.893266725994716E-5	0.0	0.0	23.01193017672268	0.0
34	6.893266725994716E-5	0.0	0.0	23.6624477576548	0.0
35	6.893266725994716E-5	0.0	0.0	24.36783574172584	0.0
36	6.893266725994716E-5	0.0	0.0	24.972168435593797	0.0
37	6.893266725994716E-5	0.0	0.0	25.587668221557866	0.0
38	6.893266725994716E-5	0.0	0.0	26.262450101365488	0.0
39	6.893266725994716E-5	0.0	0.0	27.31946362112952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.033899E-4	45.0	8
TACGCGG	35	1.2122291E-7	45.0	2
TTTAGGG	5230	0.0	41.085083	3
TTAGGGT	1050	0.0	40.92857	4
CGTTAGG	435	0.0	40.862072	2
CTTAGGG	2575	0.0	40.36893	3
TTAGGGA	2685	0.0	40.307262	4
TAGGGTA	590	0.0	40.042374	5
TCGTTAG	180	0.0	40.0	1
TGTTTAG	715	0.0	39.65035	1
TTTTAGG	2975	0.0	39.630253	2
TAGGGTG	525	0.0	39.42857	5
AGGGCGA	1085	0.0	39.40092	6
TAGGGAT	1395	0.0	39.35484	5
TAGGGCG	870	0.0	39.310345	5
CTAGGGG	2520	0.0	39.19643	4
GTTTAGG	1005	0.0	39.179104	2
GCTAGGG	1680	0.0	39.107143	3
TTGCGAG	185	0.0	38.91892	1
CTAGGGT	555	0.0	38.91892	4
>>END_MODULE