FastQCFastQC Report
Sat 14 Jan 2017
SRR2934310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202257
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228111.8973480711694752No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC112310.9341596680243909No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85560.711661483360047No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG76040.6324770826869797No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC75160.6251575162382087No Hit
GAATCAGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC39720.3303786128922518No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT39250.32646929899347643No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCT34560.2874593368971859No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC30260.2516932735679643No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT29030.24146251591797763No Hit
CGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTG27800.23123175826799094No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTC25110.20885717446436164No Hit
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC24060.20012360086071448TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCT23210.19305356508633345No Hit
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT19950.16593789846929566TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC19160.15936692404369449TruSeq Adapter, Index 20 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT17750.14763898234736833No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT14580.12127190775350029No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTC13730.11420187197911927No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTA12820.10663277485595843No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCATACT12650.10521876770108222No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG253.890913E-545.02
CCGTTAG253.890913E-545.01
TACCGGT253.890913E-545.040
CGTTTAT41150.044.945321
CGTTTTT99750.043.578951
TCGTTAG1450.043.4482731
GTTTATT42550.042.8319632
CGTTATT3900.042.6923071
CGTTAGG2750.042.5454562
CGTCTAT850.042.352941
CGTAGGG3250.041.538463
GCGTTAG1100.040.9090921
TTAGGGA20800.040.8894274
TAGGGAC8650.040.838155
TAGGGAT10800.040.8333325
TTTCGCG2150.040.8139531
TTTAGGG36550.040.259923
TGTAGGG13550.040.0184483
TAGGGGC12000.039.7500045
CTTAGGG16800.039.6428573
Produced by FastQC (version 0.11.5)