##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380748
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35469035624169	33.0	31.0	34.0	31.0	34.0
2	32.55549383377706	34.0	31.0	34.0	31.0	34.0
3	32.51608693259016	34.0	31.0	34.0	31.0	34.0
4	36.05402506467509	37.0	35.0	37.0	35.0	37.0
5	35.16475924643744	37.0	35.0	37.0	35.0	37.0
6	25.920488749576318	37.0	0.0	37.0	0.0	37.0
7	31.02260513866397	37.0	19.0	37.0	17.0	37.0
8	34.769580691045725	37.0	32.0	37.0	32.0	37.0
9	37.32878917803973	39.0	37.0	39.0	35.0	39.0
10	37.434064000092704	39.0	37.0	39.0	35.0	39.0
11	37.48268836891308	39.0	37.0	39.0	35.0	39.0
12	37.267828742102104	39.0	37.0	39.0	35.0	39.0
13	37.29856932619131	39.0	37.0	39.0	34.0	39.0
14	38.457123964691604	40.0	38.0	41.0	34.0	41.0
15	38.58313030328489	40.0	38.0	41.0	34.0	41.0
16	38.66607302708387	40.0	38.0	41.0	35.0	41.0
17	38.640367395064125	40.0	38.0	41.0	35.0	41.0
18	38.550157595737964	40.0	38.0	41.0	35.0	41.0
19	38.472871226320805	40.0	37.0	41.0	35.0	41.0
20	38.327086477764226	40.0	37.0	41.0	34.0	41.0
21	38.19996407744208	40.0	37.0	41.0	34.0	41.0
22	38.1811872984788	40.0	37.0	41.0	34.0	41.0
23	38.10496267240655	40.0	36.0	41.0	34.0	41.0
24	38.05870223965561	40.0	36.0	41.0	34.0	41.0
25	37.94514132919258	40.0	35.0	41.0	34.0	41.0
26	37.901315084287646	40.0	35.0	41.0	34.0	41.0
27	37.880078768899175	40.0	35.0	41.0	34.0	41.0
28	37.80031982664469	40.0	35.0	41.0	34.0	41.0
29	37.759482541347154	40.0	35.0	41.0	34.0	41.0
30	37.47208976583707	40.0	35.0	41.0	33.0	41.0
31	37.41016898087124	40.0	35.0	41.0	33.0	41.0
32	37.20724129240093	40.0	35.0	41.0	33.0	41.0
33	36.933631625756476	40.0	35.0	41.0	32.0	41.0
34	36.67780507377161	40.0	35.0	41.0	31.0	41.0
35	36.537943926045884	40.0	35.0	41.0	30.0	41.0
36	36.43538140196473	40.0	35.0	41.0	30.0	41.0
37	36.35768655830029	40.0	35.0	41.0	30.0	41.0
38	36.26534385709775	39.0	35.0	41.0	30.0	41.0
39	36.192737559641586	39.0	35.0	41.0	29.0	41.0
40	36.1105147354912	39.0	35.0	41.0	29.0	41.0
41	35.99206154924722	39.0	35.0	41.0	29.0	41.0
42	35.9560513576699	39.0	35.0	41.0	29.0	41.0
43	35.891177101107516	39.0	35.0	41.0	28.0	41.0
44	35.770518588475234	39.0	35.0	41.0	27.0	41.0
45	35.72384823298676	39.0	35.0	41.0	27.0	41.0
46	35.667404913858284	39.0	35.0	41.0	27.0	41.0
47	35.60731067508336	39.0	35.0	41.0	27.0	41.0
48	35.55528669967293	39.0	35.0	40.0	27.0	41.0
49	35.53702341049924	39.0	35.0	40.0	27.0	41.0
50	35.440966056079745	38.0	35.0	40.0	27.0	41.0
51	34.521582504555504	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	17.0
11	13.0
12	9.0
13	8.0
14	11.0
15	26.0
16	72.0
17	153.0
18	273.0
19	578.0
20	1040.0
21	1813.0
22	2994.0
23	5160.0
24	9145.0
25	14926.0
26	20218.0
27	20907.0
28	19659.0
29	19242.0
30	21009.0
31	25834.0
32	34610.0
33	54616.0
34	81099.0
35	104392.0
36	122850.0
37	203376.0
38	291055.0
39	325400.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.09563222253445	4.132180528235421	52.953978568138425	20.818208681091697
2	25.452435926034294	4.115378041467379	49.271481834483914	21.16070419801441
3	19.2244348715334	4.37856871782541	54.51610286598279	21.880893544658402
4	17.315831708610116	4.210616274656926	54.52624229765316	23.947309719079804
5	16.93965879363939	7.721901462106047	52.30027492344729	23.03816482080727
6	15.135346927897054	31.28963431415436	41.00038529840347	12.574633459545115
7	79.00420641565297	1.8237216349399021	14.733825433750402	4.438246515656731
8	80.65823741913803	2.160495615420048	12.922633239374601	4.25863372606732
9	75.68441163774997	3.96038958593458	15.103480142647319	5.251718633668128
10	38.251585372566176	24.039506122768238	22.708922989567974	14.999985515097613
11	27.088650499584283	23.343651412133134	30.47210642347481	19.09559166480777
12	24.46710044121013	20.744118405386068	34.27663845973342	20.512142693670388
13	23.03816482080727	20.942778841613386	36.47522936842928	19.543826969150054
14	19.38014757218551	22.94567871907111	37.054625463879	20.619548244864376
15	18.038048941588183	23.689116334045025	38.189300292305326	20.083534432061462
16	21.604304333593095	23.65840834098619	34.48456923348793	20.252718091932778
17	21.60589767285558	24.273220022770268	33.169412521329015	20.95146978304513
18	21.974176316025805	23.266881429486048	34.42938175539635	20.329560499091798
19	21.897985729474172	25.46098201844218	32.335299417417225	20.305732834666426
20	22.22802423034471	26.830239841013707	32.037779522403795	18.90395640623778
21	22.306749674813943	26.18276470434866	33.30651212241481	18.20397349842259
22	21.75646823316058	22.391196655725736	33.99201012784375	21.860324983269937
23	20.532421557011126	24.01821331626046	33.23988157143809	22.209483555290323
24	20.355198776315447	23.899002569621683	34.033726646716126	21.71207200734674
25	21.214153487819644	25.166359103906	31.479241686390274	22.14024572188408
26	19.687372351797723	25.70374898243561	32.03770709789187	22.571171567874803
27	19.68744477630965	24.195798219515797	33.68500262176733	22.431754382407217
28	18.35056071057137	24.253448131013045	33.88301123738727	23.51297992102831
29	19.6801299006046	22.76548653338625	33.81565644129124	23.738727124717908
30	21.08494815853436	22.844646524927068	33.51060439703697	22.559800919501605
31	20.734630794323078	24.14633227786678	32.19704102414054	22.921995903669607
32	21.38007804465406	24.02994608719332	31.70310585276966	22.886870015382964
33	21.447432840750086	23.32836984011565	30.977774365778547	24.24642295335572
34	19.403613114051225	23.880679168103086	32.62463534258242	24.091072375263263
35	19.15056186936356	24.806554128631728	31.322659891594988	24.72022411040972
36	20.09954024919826	25.287380463343055	30.418512284645715	24.194567002812967
37	19.82280618910909	25.567590900004927	31.306219527386602	23.30338338349938
38	20.712903440743712	25.235814210848034	30.57197982542796	23.479302522980298
39	20.61143669952808	24.18500696723805	30.50926019809552	24.694296135138348
40	21.512759750512043	24.23534200303024	30.290465747551327	23.96143249890639
41	18.768522568926407	24.59196029977954	30.414963483561085	26.224553647732968
42	19.681795664379017	23.90284106875404	31.72657139463537	24.688791872231572
43	19.993583188242894	23.01057108176148	33.000011587921904	23.995834142073715
44	21.226031107776365	23.272530541416682	30.448930579656825	25.05250777115013
45	20.92322422339196	22.800322723625165	29.768285016527273	26.508168036455604
46	20.82545113228482	23.371534849226652	30.17328288724662	25.629731131241908
47	18.614475632048716	23.955855811487687	32.89948636536138	24.530182191102217
48	18.94523837803857	23.80912375031505	32.56589906340621	24.67973880824017
49	20.96131951666778	22.180658599541697	32.259398528913316	24.598623354877212
50	19.708954856353223	22.429074675465763	31.381251321747346	26.480719146433675
51	18.728906360900034	22.18152769368487	30.337179557746964	28.752386387668132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	801.5
2	1388.0
3	15727.0
4	30066.0
5	19536.5
6	9007.0
7	8859.0
8	8711.0
9	8811.0
10	8911.0
11	8965.5
12	9020.0
13	8753.5
14	8487.0
15	8131.5
16	7776.0
17	7600.5
18	7425.0
19	7263.5
20	7102.0
21	7281.0
22	7460.0
23	7651.0
24	7842.0
25	8669.5
26	11172.0
27	12847.0
28	14188.0
29	15529.0
30	18313.0
31	21097.0
32	23849.0
33	26601.0
34	30244.0
35	33887.0
36	37310.0
37	40733.0
38	44397.0
39	48061.0
40	53303.0
41	58545.0
42	65058.0
43	71571.0
44	81677.0
45	91783.0
46	108773.5
47	125764.0
48	135760.0
49	145756.0
50	140927.5
51	136099.0
52	117970.0
53	99841.0
54	89384.0
55	78927.0
56	71498.0
57	64069.0
58	58589.0
59	53109.0
60	46680.0
61	40251.0
62	35286.5
63	30322.0
64	25861.0
65	21400.0
66	17931.0
67	14462.0
68	12591.5
69	10721.0
70	8660.0
71	6599.0
72	5514.0
73	4429.0
74	3376.5
75	1999.5
76	1675.0
77	1222.5
78	770.0
79	596.5
80	423.0
81	291.0
82	159.0
83	99.0
84	39.0
85	36.5
86	34.0
87	22.5
88	11.0
89	6.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1380748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.756071700954806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05017932175457	30.23456381986146
2	9.466971294683072	7.527391791646024
3	3.3489647633499104	3.994250497671306
4	1.7856135311517756	2.839559182986604
5	1.1853744153677717	2.3562915124919264
6	0.9012476331790016	2.149803931498812
7	0.7461856504330182	2.0765787154587074
8	0.6643835529743838	2.1130624135187768
9	0.5763645282781636	2.06225905609023
>10	5.147669539438823	35.79272319883342
>50	0.08378314335840586	2.276278512448752
>100	0.03770596003302477	2.63791707922564
>500	0.0027783329990611664	0.7810000307143894
>1k	0.002381428284909571	1.6405024384932114
>5k	1.984523570757976E-4	0.4140507284765244
>10k+	1.984523570757976E-4	1.1037670905843324
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13990	1.0132189219176853	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5248	0.38008383861501155	No Hit
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	2783	0.20155741670456884	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	2450	0.17744005423147455	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	2418	0.17512246984967567	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1900	0.1376065726693068	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1837	0.1330438284176403	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	1835	0.13289897939377787	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	1661	0.12029711431774662	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	1607	0.11638619067346105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.242451193121409E-5	0.0	0.0	0.2122038199584573	0.0
2	7.242451193121409E-5	0.0	0.0	0.691436815407301	0.0
3	7.242451193121409E-5	0.0	0.0	1.1203347750639507	0.0
4	7.242451193121409E-5	0.0	0.0	1.5924701683435356	0.0
5	7.242451193121409E-5	0.0	0.0	2.487709560325273	0.0
6	7.242451193121409E-5	0.0	0.0	3.4702929136960545	0.0
7	7.242451193121409E-5	0.0	0.0	4.534136569453659	0.0
8	7.242451193121409E-5	0.0	0.0	5.953729427817386	0.0
9	7.242451193121409E-5	0.0	0.0	6.745256918713625	0.0
10	7.242451193121409E-5	0.0	0.0	7.959091738680773	0.0
11	7.242451193121409E-5	0.0	0.0	9.752974474705015	0.0
12	7.242451193121409E-5	0.0	0.0	11.224423283611491	0.0
13	7.242451193121409E-5	0.0	0.0	11.79281085324766	0.0
14	7.242451193121409E-5	0.0	0.0	12.04282026843421	0.0
15	7.242451193121409E-5	0.0	0.0	12.328317694467056	0.0
16	7.242451193121409E-5	0.0	0.0	12.871573958463093	0.0
17	7.242451193121409E-5	0.0	0.0	13.643474406625973	0.0
18	1.4484902386242818E-4	0.0	0.0	14.441302830060229	0.0
19	1.4484902386242818E-4	0.0	0.0	15.039239600564331	0.0
20	2.172735357936423E-4	0.0	0.0	15.661945554148911	0.0
21	2.172735357936423E-4	0.0	0.0	16.563703152204457	0.0
22	2.172735357936423E-4	0.0	0.0	17.495299649175664	0.0
23	2.172735357936423E-4	0.0	0.0	18.485053029227636	2.8969804772485636E-4
24	2.172735357936423E-4	0.0	0.0	19.19582718932057	2.8969804772485636E-4
25	2.172735357936423E-4	0.0	0.0	19.85771480385994	2.8969804772485636E-4
26	2.172735357936423E-4	0.0	0.0	20.410603527942826	2.8969804772485636E-4
27	2.172735357936423E-4	0.0	0.0	20.981598380008517	2.8969804772485636E-4
28	2.172735357936423E-4	0.0	0.0	21.56106689997016	2.8969804772485636E-4
29	2.172735357936423E-4	0.0	0.0	22.214553271125506	2.8969804772485636E-4
30	2.172735357936423E-4	0.0	0.0	22.960960291088597	2.8969804772485636E-4
31	2.172735357936423E-4	0.0	0.0	23.612853322981458	2.8969804772485636E-4
32	2.8969804772485636E-4	0.0	0.0	24.222522864418416	2.8969804772485636E-4
33	2.8969804772485636E-4	0.0	0.0	24.819373267243552	2.8969804772485636E-4
34	2.8969804772485636E-4	0.0	0.0	25.41448548178234	2.8969804772485636E-4
35	2.8969804772485636E-4	0.0	0.0	26.07970462387054	2.8969804772485636E-4
36	2.8969804772485636E-4	0.0	0.0	26.67923473363713	2.8969804772485636E-4
37	2.8969804772485636E-4	0.0	0.0	27.296943395898456	2.8969804772485636E-4
38	2.8969804772485636E-4	0.0	0.0	27.95578918093671	2.8969804772485636E-4
39	3.621225596560705E-4	0.0	0.0	28.825897267278314	2.8969804772485636E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	25	3.891214E-5	45.000004	11
CGAATCG	20	7.033781E-4	45.0	30
CGTAAGG	30	2.1658216E-6	44.999996	2
CGTTTAT	2870	0.0	43.82404	1
CGTTTTT	7065	0.0	43.535034	1
GCGTTAG	110	0.0	42.954544	1
AGGGCGA	615	0.0	41.707317	6
TGCGTAG	125	0.0	41.399998	1
CGTAGGG	605	0.0	40.90909	3
CTAGGGA	1565	0.0	40.83067	4
TAGGGCG	480	0.0	40.781246	5
TTAGGGA	2720	0.0	40.69853	4
GTTAGGG	1780	0.0	40.57584	3
TCGTAGG	405	0.0	40.555553	2
CTTAGGG	2325	0.0	40.54839	3
GTTTATT	3130	0.0	40.327473	2
TAGGGAC	1070	0.0	40.16355	5
CGTTAGG	415	0.0	40.120483	2
GTAGGGA	825	0.0	40.090908	4
TTTAGGG	5155	0.0	40.024246	3
>>END_MODULE