SRR2934308.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708173
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3007	0.42461375963218023	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	2591	0.36587105128266684	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2407	0.3398886995126897	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2118	0.2990794622218017	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1930	0.2725322767176947	No Hit
TCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	880	0.12426342150858617	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	850	0.12002716850261165	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	845	0.11932112633494922	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	765	0.10802445165235049	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	741	0.10463544924757086	TruSeq Adapter, Index 15 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	35	1.2110468E-7	45.000004	25
GAACGTT	35	1.2110468E-7	45.000004	9
GCGATAG	35	1.2110468E-7	45.000004	9
CTATGCG	20	7.03145E-4	45.0	10
TCGCATC	20	7.03145E-4	45.0	31
TCCGATA	50	2.1827873E-11	45.0	14
CCGATAA	50	2.1827873E-11	45.0	15
GACGTGA	20	7.03145E-4	45.0	9
ATTCGAT	20	7.03145E-4	45.0	41
CGACATT	20	7.03145E-4	45.0	39
CACGACC	25	3.889283E-5	45.0	27
GACTTCG	20	7.03145E-4	45.0	9
TGCGATT	20	7.03145E-4	45.0	45
CGAGGGT	90	0.0	45.0	4
ATCCCGC	20	7.03145E-4	45.0	15
GTCCGCC	20	7.03145E-4	45.0	9
CGCAGTT	20	7.03145E-4	45.0	11
GAACGGT	25	3.889283E-5	45.0	12
CCGACAA	20	7.03145E-4	45.0	24
GCGGATA	20	7.03145E-4	45.0	13