##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708173
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32240144710403	33.0	31.0	34.0	31.0	34.0
2	32.49250536239026	34.0	31.0	34.0	31.0	34.0
3	32.550664879909284	34.0	31.0	34.0	31.0	34.0
4	36.024621102470725	37.0	35.0	37.0	35.0	37.0
5	35.138245880596976	37.0	35.0	37.0	35.0	37.0
6	26.00835106675911	37.0	0.0	37.0	0.0	37.0
7	31.071509362825186	37.0	19.0	37.0	17.0	37.0
8	34.76910444199369	37.0	32.0	37.0	32.0	37.0
9	37.34002708377755	39.0	37.0	39.0	35.0	39.0
10	37.317560822002534	39.0	37.0	39.0	34.0	39.0
11	37.41961074483213	39.0	37.0	39.0	35.0	39.0
12	37.18604493534772	39.0	37.0	39.0	34.0	39.0
13	37.153852236670986	39.0	37.0	39.0	33.0	39.0
14	38.350996154894354	40.0	38.0	41.0	34.0	41.0
15	38.46824010517204	40.0	38.0	41.0	34.0	41.0
16	38.54390523219609	40.0	38.0	41.0	35.0	41.0
17	38.535989087412254	40.0	38.0	41.0	35.0	41.0
18	38.52126669613216	40.0	38.0	41.0	35.0	41.0
19	38.53582528562936	40.0	37.0	41.0	35.0	41.0
20	38.475313235607686	40.0	37.0	41.0	34.0	41.0
21	38.35325407774654	40.0	37.0	41.0	34.0	41.0
22	38.365941655499434	40.0	37.0	41.0	34.0	41.0
23	38.29326591101327	40.0	37.0	41.0	34.0	41.0
24	38.24175025029195	40.0	37.0	41.0	34.0	41.0
25	38.113172911138946	40.0	37.0	41.0	34.0	41.0
26	38.079544122693186	40.0	37.0	41.0	34.0	41.0
27	38.05741534907431	40.0	37.0	41.0	34.0	41.0
28	38.003623408404444	40.0	36.0	41.0	34.0	41.0
29	38.00436334059616	40.0	36.0	41.0	34.0	41.0
30	37.75620222742183	40.0	36.0	41.0	33.0	41.0
31	37.76506164454166	40.0	36.0	41.0	33.0	41.0
32	37.64259580639194	40.0	36.0	41.0	33.0	41.0
33	37.56562167718905	40.0	36.0	41.0	33.0	41.0
34	37.51512695344217	40.0	36.0	41.0	33.0	41.0
35	37.48168456012867	40.0	36.0	41.0	33.0	41.0
36	37.41333544204594	40.0	36.0	41.0	33.0	41.0
37	37.371124005010074	40.0	35.0	41.0	33.0	41.0
38	37.25791437967841	40.0	35.0	41.0	33.0	41.0
39	37.2433473176752	39.0	35.0	41.0	33.0	41.0
40	37.15876911432658	39.0	35.0	41.0	32.0	41.0
41	37.07405676296611	39.0	35.0	41.0	32.0	41.0
42	37.02463098706107	39.0	35.0	41.0	32.0	41.0
43	37.005820611630206	39.0	35.0	41.0	32.0	41.0
44	36.90873557732362	39.0	35.0	41.0	32.0	41.0
45	36.873214595868525	39.0	35.0	41.0	31.0	41.0
46	36.77500695451535	39.0	35.0	41.0	31.0	41.0
47	36.70797531111748	39.0	35.0	41.0	31.0	41.0
48	36.68717813302682	39.0	35.0	41.0	31.0	41.0
49	36.70167035456026	39.0	35.0	40.0	31.0	41.0
50	36.60416310703741	39.0	35.0	40.0	31.0	41.0
51	35.62604759006627	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	7.0
12	7.0
13	5.0
14	4.0
15	9.0
16	15.0
17	31.0
18	86.0
19	153.0
20	281.0
21	496.0
22	788.0
23	1277.0
24	2050.0
25	3160.0
26	4239.0
27	5184.0
28	6131.0
29	7275.0
30	9414.0
31	12791.0
32	17692.0
33	29167.0
34	44252.0
35	61278.0
36	65514.0
37	111256.0
38	159638.0
39	165834.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.893879038031667	4.169885042214261	54.31681241730482	17.619423502449262
2	19.777229575259152	4.356138966043608	54.4402285882122	21.426402870485038
3	19.980004885811802	4.060730923093651	54.00657748883394	21.952686702260607
4	18.945935527053418	4.469105712869595	52.92873351568049	23.6562252443965
5	17.164449929607596	7.83438510081576	51.98306063631344	23.0181043332632
6	14.681299625938859	31.188000672152143	40.8867607208973	13.243938981011702
7	83.11147134951487	1.6473375855899617	10.457049336814592	4.784141728080568
8	85.43081987028593	1.8591502358886882	7.597578557781786	5.112451336043594
9	81.14147249330318	3.3486167927893327	10.174208844449026	5.335701869458451
10	36.24876972152285	26.13499808662573	19.682619924792387	17.933612267059036
11	27.61232071824258	27.26593643078739	26.007063245845295	19.11467960512474
12	26.4161440777889	22.12114836346486	31.65723629678059	19.80547126196565
13	21.68453188698242	26.405129819973368	31.71004825092174	20.200290042122475
14	18.431936828995173	27.614580053179093	31.499788893391866	22.45369422443386
15	16.232897893593798	26.31150862854133	36.375857311702084	21.07973616616279
16	18.018760952479127	24.82161844634009	33.545193053109905	23.614427548070882
17	17.948721569447013	24.480458870925606	30.576003321222355	26.994816238405022
18	19.62811346944885	24.08281592209813	33.61946868914799	22.66960191930503
19	21.436569877699377	26.21266272506859	29.81884370062118	22.531923696610857
20	23.676418050391643	25.29579636614217	30.51584852853752	20.51193705492867
21	22.206861882619076	27.428749754650344	29.802887147632006	20.56150121509857
22	21.261471420119097	25.0520706098651	28.54825021569588	25.138207754319918
23	19.913636922051534	26.63544077506485	28.974134851229856	24.476787451653763
24	21.59782990879347	23.524336567477157	31.40164903208679	23.47618449164258
25	19.082625290712862	26.62230839074633	28.432318091765712	25.862748226775096
26	18.220830220864112	28.02577901162569	28.374422634017392	25.37896813349281
27	18.26460483525918	27.357580704149974	29.79413222475299	24.583682235837852
28	16.446546253528446	26.480111498179117	32.298040168151005	24.775302080141433
29	18.47712352772557	24.51971481544764	30.786121470318694	26.2170401865081
30	20.830926906278552	26.949770748108158	28.55022713376534	23.669075211847954
31	21.58963981964859	28.98500790061186	25.942672200154483	23.482680079585073
32	21.836895786763968	27.543975836412855	27.33301043671532	23.286117940107857
33	21.076629580625074	26.648714367816908	28.276988814879978	23.997667236678044
34	19.899939703998882	25.75641827632513	29.096703771536053	25.246938248139934
35	19.015692493218463	24.057398404062283	28.63664669508722	28.29026240763203
36	19.39441351195259	26.02683242653984	29.275332439954642	25.303421621552925
37	20.061482151960046	27.481138083490897	29.930680779978903	22.526698984570153
38	19.987065307488425	28.12744908376908	29.228451240021858	22.657034368720637
39	21.340548142897287	26.29823503578928	30.209426227772028	22.15179059354141
40	22.877743150331913	24.397287103574975	30.619213101883297	22.10575664420982
41	19.186554697792772	23.984534852359523	30.218463567518107	26.6104468823296
42	19.361088321638924	23.79291500805594	30.300082042099884	26.545914628205253
43	20.992751771106775	23.740244262348327	30.097306731547235	25.169697234997663
44	20.65130977882523	24.468314945641815	27.893607917839287	26.98676735769367
45	21.059825777034707	24.597379453890504	26.3887496416836	27.954045127391186
46	21.589074985914458	25.18099390968026	28.624785186670486	24.60514591773479
47	18.616072626321532	24.960143919635456	31.55937885234258	24.864404601700432
48	18.99098101735028	22.963174252619066	31.94756083612338	26.098283893907283
49	20.406736771946967	22.503540801470827	32.42117392219133	24.668548504390877
50	20.535942488629193	22.71634191080428	30.084174347228714	26.663541253337815
51	18.841300077805847	22.897935956327057	28.451804855593192	29.808959110273904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	318.5
2	555.0
3	2388.0
4	4221.0
5	2792.0
6	1363.0
7	1418.0
8	1473.0
9	1535.5
10	1598.0
11	1673.0
12	1748.0
13	1728.5
14	1709.0
15	1664.0
16	1619.0
17	1623.0
18	1627.0
19	1543.0
20	1459.0
21	1591.0
22	1723.0
23	2044.0
24	2365.0
25	3039.5
26	5189.5
27	6665.0
28	6804.0
29	6943.0
30	9703.0
31	12463.0
32	13977.5
33	15492.0
34	18210.5
35	20929.0
36	22150.0
37	23371.0
38	25649.5
39	27928.0
40	32263.0
41	36598.0
42	42573.0
43	48548.0
44	53179.5
45	57811.0
46	64694.0
47	71577.0
48	80652.0
49	89727.0
50	83712.5
51	77698.0
52	64649.5
53	51601.0
54	44091.0
55	36581.0
56	32148.0
57	27715.0
58	24984.5
59	22254.0
60	19507.0
61	16760.0
62	14300.0
63	11840.0
64	10161.5
65	8483.0
66	6689.0
67	4895.0
68	3797.0
69	2699.0
70	2279.0
71	1859.0
72	1489.0
73	1119.0
74	938.5
75	504.0
76	250.0
77	197.5
78	145.0
79	121.0
80	97.0
81	80.5
82	64.0
83	52.0
84	40.0
85	22.5
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	708173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.17503793281592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94292512597059	23.203591941861777
2	12.382511420414472	8.215805721515528
3	4.622225521540437	4.600275210334013
4	2.317717253576451	3.075614312197627
5	1.4905094486604349	2.472385374926523
6	1.0109651429904447	2.0123284180477596
7	0.7865405842109253	1.8265459601827645
8	0.6604060344004699	1.7527196193836894
9	0.547658429061535	1.6351730242508515
>10	6.015679404309693	42.009276847415144
>50	0.17086058676066912	3.7764548061488608
>100	0.04500931054368335	2.5445042117660512
>500	0.004806819572626378	1.1280074790195787
>1k	0.0021849179875574444	1.7473170729498753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	3007	0.42461375963218023	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	2591	0.36587105128266684	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2407	0.3398886995126897	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	2118	0.2990794622218017	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1930	0.2725322767176947	No Hit
TCCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTG	880	0.12426342150858617	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	850	0.12002716850261165	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	845	0.11932112633494922	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	765	0.10802445165235049	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	741	0.10463544924757086	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3931242789544363	0.0
2	0.0	0.0	0.0	1.5665663616093808	0.0
3	0.0	0.0	0.0	2.206946607679197	0.0
4	0.0	0.0	0.0	2.8940668452482656	0.0
5	0.0	0.0	0.0	3.967392148528679	0.0
6	0.0	0.0	0.0	4.667362353549203	0.0
7	0.0	0.0	0.0	5.5099530764375375	0.0
8	0.0	0.0	0.0	6.934322545479707	0.0
9	0.0	0.0	0.0	7.538977057865804	0.0
10	0.0	0.0	0.0	8.818043048803046	0.0
11	0.0	0.0	0.0	11.03896929140196	0.0
12	0.0	0.0	0.0	12.94824852119468	0.0
13	0.0	0.0	0.0	13.596112814241717	0.0
14	0.0	0.0	0.0	13.799311750094963	0.0
15	0.0	0.0	0.0	14.12380873035261	0.0
16	0.0	0.0	0.0	14.956656071327204	0.0
17	0.0	0.0	0.0	16.1329223226528	0.0
18	0.0	0.0	0.0	17.51139905079691	0.0
19	0.0	0.0	0.0	18.330690382152383	0.0
20	0.0	0.0	0.0	19.1814711941856	0.0
21	0.0	0.0	0.0	20.43766141889058	0.0
22	0.0	0.0	0.0	21.68396705324829	0.0
23	0.0	0.0	0.0	22.946229240595166	0.0
24	0.0	0.0	0.0	23.854623093509637	0.0
25	0.0	0.0	0.0	24.69664898266384	0.0
26	0.0	0.0	0.0	25.40367960936099	0.0
27	0.0	0.0	0.0	26.06933616503312	0.0
28	0.0	0.0	0.0	26.765352533914736	0.0
29	0.0	0.0	0.0	27.55569613639605	0.0
30	0.0	0.0	0.0	28.415937913475943	0.0
31	0.0	0.0	0.0	29.202186471384817	0.0
32	0.0	0.0	0.0	29.908228639047238	0.0
33	0.0	0.0	0.0	30.615824099478516	0.0
34	0.0	0.0	0.0	31.30435642138291	0.0
35	0.0	0.0	0.0	32.06179845885116	0.0
36	0.0	0.0	0.0	32.75993295423576	0.0
37	0.0	0.0	0.0	33.473882794176	0.0
38	0.0	0.0	0.0	34.13840968237987	0.0
39	0.0	0.0	0.0	34.88102483432721	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	35	1.2110468E-7	45.000004	25
GAACGTT	35	1.2110468E-7	45.000004	9
GCGATAG	35	1.2110468E-7	45.000004	9
CTATGCG	20	7.03145E-4	45.0	10
TCGCATC	20	7.03145E-4	45.0	31
TCCGATA	50	2.1827873E-11	45.0	14
CCGATAA	50	2.1827873E-11	45.0	15
GACGTGA	20	7.03145E-4	45.0	9
ATTCGAT	20	7.03145E-4	45.0	41
CGACATT	20	7.03145E-4	45.0	39
CACGACC	25	3.889283E-5	45.0	27
GACTTCG	20	7.03145E-4	45.0	9
TGCGATT	20	7.03145E-4	45.0	45
CGAGGGT	90	0.0	45.0	4
ATCCCGC	20	7.03145E-4	45.0	15
GTCCGCC	20	7.03145E-4	45.0	9
CGCAGTT	20	7.03145E-4	45.0	11
GAACGGT	25	3.889283E-5	45.0	12
CCGACAA	20	7.03145E-4	45.0	24
GCGGATA	20	7.03145E-4	45.0	13
>>END_MODULE