FastQCFastQC Report
Sat 14 Jan 2017
SRR2934306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468847
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC202221.3767260987699876No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG185421.2623506736916779No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC162041.1031782071243634No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147621.005005967265481No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53390.3634823776744617No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT48970.3333907479812397No Hit
GAATCAGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC47900.326106122693514No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC46080.3137154516433638No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC43430.29567408994946376No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT37370.25441724018907347No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT36710.24992391991813986TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA34090.2320868000547368No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT33810.23018054297009832No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC32780.22316824012303527No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG27890.18987682175202727TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC21290.14494361904269132No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT18760.1277192246707792No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC18290.12451943599299314No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT17000.11573703728162293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.0339267E-445.00000429
GCGATCG253.891338E-545.09
CGTTTAT33600.044.5982131
CGTTTTT78750.044.0285721
TACGGCT25500.042.176477
TAGGGAC10850.041.88945
GTTTATT36200.041.457182
CCGATGA4950.041.36363618
TCGTTAG1850.041.3513531
CGGGTCG603.6379788E-1241.2500046
TTTAGGG46350.041.0194173
CGTTAGG3900.040.9615362
TTAGGGA26250.040.8000034
GCGTTAG1550.040.645161
TAGGGCG5500.040.55
GTAGGGC4450.040.449444
AGGGCGA7300.040.3767136
CCGTAGG1400.040.1785742
TTAGGGT10150.040.123154
GTTAGGG17900.039.9720653
Produced by FastQC (version 0.11.5)