##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1468847
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17412160694749	33.0	31.0	34.0	30.0	34.0
2	32.34204992078821	34.0	31.0	34.0	30.0	34.0
3	32.31922930026068	34.0	31.0	34.0	30.0	34.0
4	35.94232891512867	37.0	35.0	37.0	35.0	37.0
5	35.09457826444824	37.0	35.0	37.0	35.0	37.0
6	26.161604986768534	35.0	0.0	37.0	0.0	37.0
7	31.11061533297886	37.0	19.0	37.0	17.0	37.0
8	34.53838418841445	37.0	32.0	37.0	32.0	37.0
9	37.14705207553952	39.0	37.0	39.0	34.0	39.0
10	37.27889766599244	39.0	37.0	39.0	34.0	39.0
11	37.32356058867942	39.0	37.0	39.0	34.0	39.0
12	37.12083763659523	39.0	37.0	39.0	34.0	39.0
13	37.10036511631232	39.0	37.0	39.0	33.0	39.0
14	38.16630391048217	40.0	38.0	41.0	33.0	41.0
15	38.25895208963221	40.0	38.0	41.0	33.0	41.0
16	38.4326631705004	40.0	38.0	41.0	34.0	41.0
17	38.37678941373744	40.0	38.0	41.0	34.0	41.0
18	38.28241743353801	40.0	37.0	41.0	34.0	41.0
19	38.21854352427448	40.0	37.0	41.0	34.0	41.0
20	38.00243115858902	40.0	36.0	41.0	34.0	41.0
21	37.949096127779136	40.0	36.0	41.0	34.0	41.0
22	37.96932151544715	40.0	36.0	41.0	34.0	41.0
23	37.87384594855693	40.0	35.0	41.0	34.0	41.0
24	37.790110202083675	39.0	35.0	41.0	33.0	41.0
25	37.68835828374228	39.0	35.0	41.0	33.0	41.0
26	37.65419339114285	39.0	35.0	41.0	33.0	41.0
27	37.58005360667244	39.0	35.0	41.0	33.0	41.0
28	37.515533612418444	39.0	35.0	41.0	33.0	41.0
29	37.44958392535097	39.0	35.0	41.0	33.0	41.0
30	37.11951346872751	39.0	35.0	41.0	32.0	41.0
31	37.042738964643696	39.0	35.0	41.0	32.0	41.0
32	36.76324831653671	39.0	35.0	41.0	31.0	41.0
33	36.51272869127962	39.0	35.0	41.0	30.0	41.0
34	36.22592141999814	39.0	35.0	41.0	30.0	41.0
35	35.90936836852307	39.0	35.0	41.0	29.0	41.0
36	35.83676175939359	39.0	35.0	41.0	28.0	41.0
37	35.68995681646897	39.0	35.0	41.0	27.0	41.0
38	35.608822430110145	39.0	35.0	40.0	26.0	41.0
39	35.62072564399151	39.0	35.0	41.0	26.0	41.0
40	35.44223530428969	39.0	35.0	40.0	25.0	41.0
41	35.25691852180656	38.0	35.0	40.0	24.0	41.0
42	35.12784857783009	38.0	35.0	40.0	23.0	41.0
43	35.22739808843263	38.0	35.0	40.0	24.0	41.0
44	35.203556939558716	38.0	35.0	40.0	24.0	41.0
45	35.17214863086489	38.0	35.0	40.0	24.0	41.0
46	35.08158916483473	38.0	34.0	40.0	23.0	41.0
47	34.939866439458974	38.0	34.0	40.0	23.0	41.0
48	34.891302497809505	38.0	34.0	40.0	23.0	41.0
49	34.85878719839439	38.0	34.0	40.0	24.0	41.0
50	34.76566245497319	38.0	34.0	40.0	23.0	41.0
51	33.759403804480655	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	15.0
11	13.0
12	7.0
13	13.0
14	10.0
15	29.0
16	63.0
17	157.0
18	405.0
19	841.0
20	1598.0
21	2884.0
22	4672.0
23	7147.0
24	11939.0
25	18658.0
26	23977.0
27	24662.0
28	23328.0
29	23307.0
30	26922.0
31	33831.0
32	45363.0
33	66268.0
34	94256.0
35	113531.0
36	139834.0
37	221485.0
38	296855.0
39	286625.0
40	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.4025817528987	3.743276188738514	49.12717253737115	19.726969520991634
2	24.764390028369192	10.962339848874661	45.29886366653572	18.974406456220425
3	17.393710849394118	10.84408382901691	51.528307577303835	20.23389774428514
4	16.480954108903106	3.837976317478948	57.8347506581693	21.84631891544865
5	20.66525649029477	7.142541054309945	49.74554871950584	22.446653735889445
6	14.169072748897605	33.63331919525995	40.28111845549604	11.916489600346395
7	73.69474152175141	1.583486911843099	20.101276715682438	4.62049485072305
8	74.02357086885155	6.16333763829725	14.896173665466858	4.916917827384336
9	69.62433800116689	3.5371281011568936	16.058309680994682	10.78022421668152
10	38.2294411875437	21.537437187127047	24.760849836640574	15.47227178868868
11	27.906650590565253	21.85802878039714	30.807905792774875	19.42741483626273
12	23.765987880289778	18.9353962665955	35.24376602872866	22.054849824386068
13	22.95814336006405	20.186037075338685	39.10638752708757	17.749432037509692
14	19.45430667727816	24.076095059594362	36.31855462141394	20.151043641713535
15	15.991999166693333	22.830083732342445	41.69134021446754	19.486576886496685
16	19.621989220116188	23.766396363950772	33.75205177938887	22.859562636544172
17	20.028158140364518	24.070240127120115	34.59298347615511	21.308618256360262
18	19.847608362205186	23.32714026716193	35.41859703563407	21.406654334998812
19	19.905953445117156	26.097340294802656	32.15474450368214	21.841961756398046
20	19.85754813128937	27.49421825418168	34.91303042454388	17.735203189985068
21	20.341873592007882	27.459973707268354	34.3651857545408	17.83296694618296
22	19.236516805358217	21.8113254818235	35.57348042376095	23.378677289057332
23	18.462440267774653	25.605253644525263	34.50223202280428	21.4300740648958
24	19.737181612516483	24.306071360733963	32.710758846905094	23.245988179844463
25	19.582434385609936	28.527069191004916	29.96002987377174	21.930466549613403
26	19.0369044563525	23.96171963451605	32.86965899103174	24.13171691809971
27	20.3586895027188	24.557356892855417	33.33376451053105	21.750189093894733
28	17.976753194852833	23.237682345404252	35.574365471693106	23.211198988049812
29	22.868004632204716	21.774970435995037	32.74262057246261	22.61440435933763
30	22.480489799141775	23.37622638709137	34.931752592339436	19.211531221427418
31	23.13903354127421	22.359919038538393	33.36208604435996	21.138961375827435
32	25.817188583970964	23.85653509181011	31.292231253493384	19.03404507072554
33	25.002059438457515	21.262323441447613	31.358405606574408	22.377211513520468
34	25.833459849800555	21.75073373877606	32.06229103507717	20.35351537634621
35	22.7270777691618	22.46251651805804	33.33430915541237	21.476096557367786
36	25.217602650241993	22.434331145449455	33.405521473645656	18.942544730662895
37	22.28618773772898	23.10438051069989	35.900539674996786	18.708892076574347
38	23.35961471821095	22.684118904147265	33.27787032958504	20.67839604805674
39	26.91403529434992	21.560039949702045	30.710959003898974	20.814965752049055
40	25.51994863998769	21.316107123478485	33.17806415508218	19.985880081451644
41	22.51854686022438	22.53236722408801	32.492764733154644	22.456321182532964
42	20.339899254313075	21.761626636402568	36.831814341452855	21.066659767831503
43	21.825554329348122	21.802066518840967	34.33019232091566	22.042186830895254
44	22.675813069707058	21.48310886021485	31.835242200174697	24.0058358699034
45	22.15499640193975	20.53106960765825	30.378861787510886	26.935072202891114
46	24.96189187845977	22.468711853583116	30.291786687109006	22.277609580848107
47	20.042591229719637	21.62151674068164	36.87518169012838	21.460710339470346
48	21.376494624695425	23.22849146303189	32.39248199438063	23.00253191789206
49	22.631356431268877	19.884508052914974	35.56381297711743	21.920322538698723
50	22.602217930117977	20.045791018397423	32.77305260520667	24.57893844627793
51	21.276552288972233	20.21367780306594	30.49419034113151	28.01557956683031
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	749.0
2	1279.0
3	15892.5
4	30506.0
5	20083.0
6	9660.0
7	9482.0
8	9304.0
9	9616.0
10	9928.0
11	9877.0
12	9826.0
13	9674.5
14	9523.0
15	9204.0
16	8885.0
17	8607.0
18	8329.0
19	8082.5
20	7836.0
21	7735.0
22	7634.0
23	7814.5
24	7995.0
25	8990.5
26	11157.5
27	12329.0
28	13516.5
29	14704.0
30	16977.0
31	19250.0
32	22417.0
33	25584.0
34	29153.5
35	32723.0
36	35935.0
37	39147.0
38	43956.5
39	48766.0
40	54816.5
41	60867.0
42	67848.0
43	74829.0
44	87274.0
45	99719.0
46	153790.5
47	207862.0
48	186553.0
49	165244.0
50	157126.5
51	149009.0
52	125947.0
53	102885.0
54	89273.0
55	75661.0
56	67303.5
57	58946.0
58	52588.0
59	46230.0
60	39378.5
61	32527.0
62	28175.5
63	23824.0
64	19962.0
65	16100.0
66	13568.5
67	11037.0
68	9673.0
69	8309.0
70	6639.0
71	4969.0
72	4151.0
73	3333.0
74	2718.5
75	1533.0
76	962.0
77	719.0
78	476.0
79	358.5
80	241.0
81	179.0
82	117.0
83	94.0
84	71.0
85	61.5
86	52.0
87	50.0
88	48.0
89	25.5
90	3.0
91	6.0
92	9.0
93	4.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1468847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.96318186218444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46225294781942	30.55674920146056
2	10.128437192943435	8.095291550426227
3	3.5498230254299608	4.255866694314818
4	1.7978829260598537	2.8739648936418494
5	1.2124027124648307	2.422573504421887
6	0.8490548764747405	2.0358560663720757
7	0.7154346308107381	2.0013730983116016
8	0.5935723549449744	1.8976831975224884
9	0.4970889838484163	1.7878731716900453
>10	4.054108800293857	28.042886590817172
>50	0.09175955590338292	2.537889611771558
>100	0.0398106072404365	2.825512149494713
>500	0.003720615896011782	1.0721866419265111
>1k	0.003720615896011782	4.013377040477103
>5k	1.8603079480058915E-4	0.3969216808518682
>10k+	7.441231792023566E-4	5.183994906499488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	20222	1.3767260987699876	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	18542	1.2623506736916779	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	16204	1.1031782071243634	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14762	1.005005967265481	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5339	0.3634823776744617	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	4897	0.3333907479812397	No Hit
GAATCAGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	4790	0.326106122693514	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	4608	0.3137154516433638	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC	4343	0.29567408994946376	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	3737	0.25441724018907347	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	3671	0.24992391991813986	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA	3409	0.2320868000547368	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT	3381	0.23018054297009832	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	3278	0.22316824012303527	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	2789	0.18987682175202727	TruSeq Adapter, Index 21 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	2129	0.14494361904269132	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	1876	0.1277192246707792	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	1829	0.12451943599299314	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	1700	0.11573703728162293	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4040305082830275E-4	0.0	0.0	0.2695992162560158	0.0
2	3.4040305082830275E-4	0.0	0.0	1.0210049106544112	0.0
3	3.4040305082830275E-4	0.0	0.0	1.4886506218823337	0.0
4	3.4040305082830275E-4	0.0	0.0	2.232227046111678	0.0
5	3.4040305082830275E-4	0.0	0.0	4.281725734538723	0.0
6	3.4040305082830275E-4	0.0	0.0	5.325333407768134	0.0
7	3.4040305082830275E-4	0.0	0.0	6.683745822403559	0.0
8	3.4040305082830275E-4	0.0	0.0	8.315501886854111	0.0
9	3.4040305082830275E-4	0.0	0.0	9.094548308979764	0.0
10	3.4040305082830275E-4	0.0	0.0	11.604544244567338	0.0
11	3.4040305082830275E-4	0.0	0.0	13.549403035169762	0.0
12	3.4040305082830275E-4	0.0	0.0	16.61834078021741	0.0
13	3.4040305082830275E-4	0.0	0.0	17.319366823093215	0.0
14	3.4040305082830275E-4	0.0	0.0	17.685232022123476	0.0
15	3.4040305082830275E-4	0.0	0.0	18.23757001239748	0.0
16	3.4040305082830275E-4	0.0	0.0	18.902036767614327	0.0
17	3.4040305082830275E-4	0.0	0.0	19.72628871488998	0.0
18	3.4040305082830275E-4	0.0	0.0	20.58948277118039	0.0
19	4.084836609939633E-4	0.0	0.0	21.537641428957542	0.0
20	4.084836609939633E-4	0.0	0.0	22.258955493662718	0.0
21	4.084836609939633E-4	0.0	0.0	23.243469197268332	0.0
22	4.7656427115962385E-4	0.0	0.0	24.337865005681326	0.0
23	4.7656427115962385E-4	0.0	0.0	25.389233868469624	0.0
24	4.7656427115962385E-4	0.0	0.0	26.172569369035713	0.0
25	4.7656427115962385E-4	0.0	0.0	26.875025104724998	0.0
26	4.7656427115962385E-4	0.0	0.0	27.47917243933507	0.0
27	4.7656427115962385E-4	0.0	0.0	28.133222861196572	0.0
28	4.7656427115962385E-4	0.0	0.0	28.768687276482847	0.0
29	4.7656427115962385E-4	0.0	0.0	29.468079384714677	0.0
30	4.7656427115962385E-4	0.0	0.0	30.28463822304161	0.0
31	4.7656427115962385E-4	0.0	0.0	30.968303710325173	0.0
32	4.7656427115962385E-4	0.0	0.0	31.612278201882155	0.0
33	4.7656427115962385E-4	0.0	0.0	32.24352161933816	0.0
34	4.7656427115962385E-4	0.0	0.0	32.91690693448671	0.0
35	5.446448813252844E-4	0.0	0.0	33.626102650582396	0.0
36	5.446448813252844E-4	0.0	0.0	34.282808216240355	0.0
37	6.127254914909449E-4	0.0	0.0	34.92412756400088	0.0
38	6.127254914909449E-4	0.0	0.0	35.63025965263911	0.0
39	6.127254914909449E-4	0.0	0.0	36.6800626613936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	20	7.0339267E-4	45.000004	29
GCGATCG	25	3.891338E-5	45.0	9
CGTTTAT	3360	0.0	44.598213	1
CGTTTTT	7875	0.0	44.028572	1
TACGGCT	2550	0.0	42.17647	7
TAGGGAC	1085	0.0	41.8894	5
GTTTATT	3620	0.0	41.45718	2
CCGATGA	495	0.0	41.363636	18
TCGTTAG	185	0.0	41.351353	1
CGGGTCG	60	3.6379788E-12	41.250004	6
TTTAGGG	4635	0.0	41.019417	3
CGTTAGG	390	0.0	40.961536	2
TTAGGGA	2625	0.0	40.800003	4
GCGTTAG	155	0.0	40.64516	1
TAGGGCG	550	0.0	40.5	5
GTAGGGC	445	0.0	40.44944	4
AGGGCGA	730	0.0	40.376713	6
CCGTAGG	140	0.0	40.178574	2
TTAGGGT	1015	0.0	40.12315	4
GTTAGGG	1790	0.0	39.972065	3
>>END_MODULE