##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934303.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1268269 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2141383255445 33.0 31.0 34.0 30.0 34.0 2 32.38316477024984 34.0 31.0 34.0 30.0 34.0 3 32.40088498575618 34.0 31.0 34.0 30.0 34.0 4 35.953925389645256 37.0 35.0 37.0 35.0 37.0 5 35.04378329833813 37.0 35.0 37.0 35.0 37.0 6 25.667595754528417 36.0 0.0 37.0 0.0 37.0 7 30.895957403358437 37.0 19.0 37.0 17.0 37.0 8 34.705247072979 37.0 32.0 37.0 32.0 37.0 9 37.27951562326289 39.0 37.0 39.0 35.0 39.0 10 37.237537147087885 39.0 37.0 39.0 34.0 39.0 11 37.261513921731115 39.0 37.0 39.0 34.0 39.0 12 37.19352597911011 39.0 37.0 39.0 34.0 39.0 13 37.277516047463116 39.0 37.0 39.0 34.0 39.0 14 38.478928366143144 40.0 38.0 41.0 34.0 41.0 15 38.57793733032977 40.0 38.0 41.0 34.0 41.0 16 38.61528114303827 40.0 38.0 41.0 35.0 41.0 17 38.60470531093956 40.0 38.0 41.0 35.0 41.0 18 38.56239488625836 40.0 38.0 41.0 35.0 41.0 19 38.55439421763049 40.0 38.0 41.0 35.0 41.0 20 38.46956994139256 40.0 38.0 41.0 34.0 41.0 21 38.37116810392748 40.0 37.0 41.0 34.0 41.0 22 38.3539990333281 40.0 37.0 41.0 34.0 41.0 23 38.28016296227378 40.0 37.0 41.0 34.0 41.0 24 38.22762757743034 40.0 37.0 41.0 34.0 41.0 25 38.100999866747514 40.0 37.0 41.0 34.0 41.0 26 38.0655933402141 40.0 37.0 41.0 34.0 41.0 27 38.0310604453787 40.0 37.0 41.0 34.0 41.0 28 37.968679357454924 40.0 37.0 41.0 34.0 41.0 29 37.93788226314764 40.0 37.0 41.0 34.0 41.0 30 37.639121511288224 40.0 36.0 41.0 33.0 41.0 31 37.63179183595909 40.0 36.0 41.0 33.0 41.0 32 37.50085194860081 40.0 36.0 41.0 33.0 41.0 33 37.32295987680847 40.0 36.0 41.0 32.0 41.0 34 37.233739056935086 40.0 36.0 41.0 32.0 41.0 35 37.161561151459196 40.0 36.0 41.0 32.0 41.0 36 37.051949546980964 40.0 36.0 41.0 31.0 41.0 37 36.981063165621805 40.0 36.0 41.0 31.0 41.0 38 36.9087764504218 40.0 36.0 41.0 31.0 41.0 39 36.804673141108076 39.0 36.0 41.0 31.0 41.0 40 36.739682985234204 39.0 35.0 41.0 31.0 41.0 41 36.7375769651391 39.0 35.0 41.0 31.0 41.0 42 36.73408638072838 39.0 35.0 41.0 31.0 41.0 43 36.6856589572086 39.0 35.0 41.0 31.0 41.0 44 36.5340002791206 39.0 35.0 41.0 30.0 41.0 45 36.48216821510263 39.0 35.0 41.0 30.0 41.0 46 36.36418141577221 39.0 35.0 41.0 30.0 41.0 47 36.281426889721345 39.0 35.0 40.0 30.0 41.0 48 36.265625036959825 39.0 35.0 40.0 30.0 41.0 49 36.23650739709005 39.0 35.0 40.0 29.0 41.0 50 36.136548319008035 39.0 35.0 40.0 29.0 41.0 51 35.17191778715714 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 2.0 10 5.0 11 4.0 12 6.0 13 2.0 14 10.0 15 7.0 16 35.0 17 73.0 18 144.0 19 321.0 20 687.0 21 1177.0 22 2059.0 23 3332.0 24 5846.0 25 9267.0 26 11983.0 27 13049.0 28 13249.0 29 14950.0 30 17968.0 31 23633.0 32 31664.0 33 47488.0 34 69948.0 35 96886.0 36 126710.0 37 211445.0 38 286945.0 39 279226.0 40 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.303889001465777 4.304291912835526 58.16502650462954 14.226792581069159 2 22.064010079880532 4.400012931010693 51.817398359496295 21.718578629612487 3 18.292728119980854 4.459227498267324 55.24096228796888 22.00708209378294 4 16.973213095959927 3.831915784427436 55.394636311381895 23.800234808230748 5 16.65805913414268 7.599807296401631 53.05294066164197 22.68919290781372 6 14.225846409555071 31.682553149213614 41.733260057606074 12.35834038362524 7 81.24979795295792 1.0890434127144952 12.79129269894636 4.869865935381217 8 83.0554874399674 1.5482519875515366 10.248772145341405 5.147488427139669 9 78.60446009482216 3.5389180055650655 12.340205429605234 5.516416470007545 10 36.763651875114824 29.720903057632096 20.09534254956953 13.420102517683553 11 22.207512759517105 25.924547552609106 31.341221775506618 20.52671791236717 12 20.295378977172824 22.326336132161238 36.10101642474901 21.277268465916933 13 21.017544385299963 23.948626040690105 36.82767614756806 18.20615342644187 14 19.576367474092642 26.909669794026346 33.94548002040577 19.568482711475248 15 17.02548907211325 26.42728001709417 36.01262823580802 20.534602674984566 16 18.194720520646644 26.971486332946714 32.99710077278558 21.83669237362105 17 18.756352161883637 26.556274733514734 31.23209666088188 23.455276443719747 18 19.426635831988325 25.656544471243876 33.66383629971244 21.252983397055356 19 19.666884548940327 27.70129996081273 31.93368283857762 20.698132651669322 20 21.126748347550876 28.330819408185487 31.50703833335042 19.03539391091322 21 21.023536804889183 27.899207502509327 32.0305865711454 19.046669121456095 22 19.97013251920531 25.514382201252257 31.321352173710782 23.19413310583165 23 18.870523524583508 27.248793434200476 29.454871166921215 24.425811874294805 24 20.095657940074226 25.905781817579708 30.89730964014732 23.101250602198746 25 18.795539432092088 27.993588111039536 28.91783998505049 24.293032471817888 26 19.272252179939745 28.246215905300847 30.312969882572233 22.16856203218718 27 19.588431160897255 26.648447608512075 31.819905714008623 21.94321551658205 28 17.30066728746031 24.976877933624493 32.65490207519067 25.067552703724527 29 18.917437862157 23.411437163567033 31.72623473411398 25.944890240161982 30 20.293407786518475 26.34914201955579 30.696957822039334 22.660492371886406 31 19.457228710943813 28.14970641086394 27.86435685174044 24.528708026451802 32 18.874308210639857 28.45563520041884 27.23491625199386 25.43514033694745 33 18.06588349947842 27.728423544216568 28.861542779962296 25.344150176342715 34 18.055712155701983 26.941918473131487 28.18983985258648 26.812529518580046 35 18.43354998032752 25.803674141684454 27.94028711574595 27.82248876224208 36 19.56935003536316 26.690788783767484 30.184684794787227 23.55517638608213 37 16.611460187073877 27.19628091516863 31.201030696169347 24.991228201588147 38 16.287396443498974 27.868693471180013 28.201824691764916 27.642085393556098 39 17.9370464783102 28.139613914713678 29.224005317483908 24.699334289492214 40 19.110614546283163 28.50491496677755 28.657169732919435 23.727300754019847 41 18.323084456057824 27.0771421520198 28.046573715828426 26.553199676093953 42 19.474575188702083 24.414221273247236 30.34356276152772 25.76764077652296 43 19.632664679180838 23.4142756781093 31.860196850983506 25.09286279172636 44 18.575869945571483 24.523977168881363 28.61727283407542 28.282880051471732 45 20.224652656494797 24.372195488496526 27.028098928539606 28.375052926469067 46 21.163175950843236 25.373166102774725 28.47014316363484 24.99351478274719 47 17.578920560228152 25.80107217002071 30.920490842242458 25.699516427508673 48 17.687099503338803 24.23452753319682 30.956681902656296 27.12169106080808 49 19.46172302563573 22.8717251624064 31.5527699565313 26.11378185542657 50 18.834647854674362 23.065690322794296 29.58102736879952 28.518634453731817 51 17.668728006440272 22.94852275029982 27.411061848866446 31.97168739439346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 99.0 1 344.5 2 590.0 3 7042.5 4 13495.0 5 8817.5 6 4140.0 7 4231.5 8 4323.0 9 4768.5 10 5214.0 11 5631.0 12 6048.0 13 6193.5 14 6339.0 15 6099.0 16 5859.0 17 5749.5 18 5640.0 19 5476.0 20 5312.0 21 5241.0 22 5170.0 23 5619.5 24 6069.0 25 6802.5 26 8547.0 27 9558.0 28 10810.0 29 12062.0 30 14467.5 31 16873.0 32 19949.5 33 23026.0 34 26602.0 35 30178.0 36 35483.5 37 40789.0 38 45609.0 39 50429.0 40 57485.0 41 64541.0 42 73757.5 43 82974.0 44 96452.0 45 109930.0 46 132325.0 47 154720.0 48 169534.5 49 184349.0 50 168234.5 51 152120.0 52 123776.5 53 95433.0 54 77000.5 55 58568.0 56 49259.5 57 39951.0 58 33167.0 59 26383.0 60 21655.0 61 16927.0 62 13946.0 63 10965.0 64 8485.0 65 6005.0 66 4630.0 67 3255.0 68 2447.5 69 1640.0 70 1227.0 71 814.0 72 634.5 73 455.0 74 335.0 75 156.0 76 97.0 77 81.5 78 66.0 79 52.5 80 39.0 81 37.5 82 36.0 83 33.5 84 31.0 85 17.5 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1268269.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.65908431897344 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6907967074685 31.435819102762284 2 12.078295478138475 10.304980504627695 3 4.395182227215772 5.624833476841533 4 2.200175382545483 3.7542986864214956 5 1.381708140988023 2.9471202045310054 6 0.9625962261593632 2.463808414611474 7 0.7287681245308381 2.176200661133881 8 0.6021185532706461 2.0548660907192637 9 0.48994243089339523 1.881044592383175 >10 3.301414481357798 25.038591190158034 >50 0.10414931069696143 3.084021352263112 >100 0.05762535185953366 4.348176247091104 >500 0.003516122371734083 1.0239628679279489 >1k 0.0033207822399710786 2.852313232935039 >5k 3.9068026352600925E-4 1.0099633755929964 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6773 0.5340349720761132 No Hit CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT 5347 0.421598257152071 No Hit TCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 4384 0.3456679931465643 No Hit CCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 4321 0.34070059269760594 No Hit GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 3628 0.28605918775906375 No Hit CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2464 0.19428055089259455 No Hit CGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG 2409 0.1899439314530277 No Hit TCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTG 1852 0.1460258036741417 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC 1634 0.12883702116822218 No Hit TCTGTATCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 1581 0.12465809698100323 No Hit GCTGTATCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC 1564 0.12331768733604621 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC 1534 0.12095225855082795 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG 1474 0.1162214009803914 No Hit CTGTCACTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT 1454 0.11464444845691252 No Hit TTCCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCT 1332 0.10502503806369154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.88476261739426E-5 0.0 0.0 0.45495080302364876 0.0 2 7.88476261739426E-5 0.0 0.0 1.535636367363706 0.0 3 7.88476261739426E-5 0.0 0.0 2.28287532061416 0.0 4 7.88476261739426E-5 0.0 0.0 3.0989482515144657 0.0 5 7.88476261739426E-5 0.0 0.0 4.502278302158296 0.0 6 7.88476261739426E-5 0.0 0.0 5.555051806832777 0.0 7 1.576952523478852E-4 0.0 0.0 6.809359844007856 0.0 8 1.576952523478852E-4 0.0 0.0 8.695395062088563 0.0 9 1.576952523478852E-4 0.0 0.0 9.57888271336759 0.0 10 1.576952523478852E-4 0.0 0.0 11.221515309449336 0.0 11 1.576952523478852E-4 0.0 0.0 14.20463639811428 0.0 12 1.576952523478852E-4 0.0 0.0 16.78855195546055 0.0 13 1.576952523478852E-4 0.0 0.0 17.656348929130964 0.0 14 1.576952523478852E-4 0.0 0.0 17.954392956068467 0.0 15 1.576952523478852E-4 0.0 0.0 18.340904019573134 0.0 16 1.576952523478852E-4 0.0 0.0 19.19718923982215 0.0 17 1.576952523478852E-4 0.0 0.0 20.664937801050094 0.0 18 1.576952523478852E-4 0.0 0.0 22.176841032935442 0.0 19 1.576952523478852E-4 0.0 0.0 23.2853598093149 0.0 20 1.576952523478852E-4 0.0 0.0 24.31487326426807 0.0 21 1.576952523478852E-4 0.0 0.0 25.770636986317573 0.0 22 1.576952523478852E-4 0.0 0.0 27.383228636827045 0.0 23 1.576952523478852E-4 0.0 0.0 28.982179648008426 0.0 24 1.576952523478852E-4 0.0 0.0 30.196669633965666 0.0 25 1.576952523478852E-4 0.0 0.0 31.193855562187515 0.0 26 1.576952523478852E-4 0.0 0.0 32.085779909467156 0.0 27 1.576952523478852E-4 0.0 0.0 32.97305224680253 0.0 28 2.3654287852182778E-4 0.0 0.0 33.909446655244274 0.0 29 2.3654287852182778E-4 0.0 0.0 34.885186029146816 0.0 30 2.3654287852182778E-4 0.0 0.0 35.9448981249246 0.0 31 2.3654287852182778E-4 0.0 0.0 36.92316062286471 0.0 32 2.3654287852182778E-4 0.0 0.0 37.80538671212495 0.0 33 2.3654287852182778E-4 0.0 0.0 38.68974168729189 0.0 34 2.3654287852182778E-4 0.0 0.0 39.5611656517663 0.0 35 2.3654287852182778E-4 0.0 0.0 40.43527043553063 0.0 36 2.3654287852182778E-4 0.0 0.0 41.31315990535131 0.0 37 2.3654287852182778E-4 0.0 0.0 42.164241182272846 0.0 38 2.3654287852182778E-4 0.0 0.0 43.05372125314109 0.0 39 2.3654287852182778E-4 0.0 0.0 43.91300268318472 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCAG 60 0.0 45.000004 1 ATTACGA 30 2.1656797E-6 45.000004 15 CCGCTAG 30 2.1656797E-6 45.000004 1 GAAGCGC 20 7.033564E-4 45.0 9 TTTCGAA 20 7.033564E-4 45.0 19 CCGTCAC 20 7.033564E-4 45.0 33 CCGAACC 20 7.033564E-4 45.0 35 ACTCCGC 20 7.033564E-4 45.0 41 GACGATA 20 7.033564E-4 45.0 9 CGTCACT 20 7.033564E-4 45.0 34 CGCCAGA 35 1.2120654E-7 45.0 16 CTTCGCG 55 1.8189894E-12 44.999996 1 CGTTTAT 1485 0.0 43.939396 1 CGTTTTT 3715 0.0 43.849262 1 TAGGGCG 480 0.0 43.593754 5 TGTTCCG 135 0.0 43.333332 1 TTAGGGA 3250 0.0 42.576923 4 AAGGGCG 75 0.0 42.0 5 TCGTTAG 115 0.0 41.086956 1 TAGGGAT 2010 0.0 41.08209 5 >>END_MODULE