FastQCFastQC Report
Sat 14 Jan 2017
SRR2934301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027242
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT49750.48430652173489785No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC49520.48206751670979187No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC42800.41664963075886696No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC33740.3284523023786021No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.2767604907120231No Hit
TCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG17770.17298747520058563No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG16570.1613057098522062No Hit
CTGTCACTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT15690.15273908193006128No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC14210.13833157133372662No Hit
CCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC13960.1358978702194809No Hit
TCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC13880.1351190858629223No Hit
TTCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT12720.12382671269282214No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.10834837360621938No Hit
TGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG10890.10601202053654349No Hit
GCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC10520.10241014288745982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCG453.8562575E-1045.0000041
GCCGATC453.8562575E-1045.0000049
TCGCTAG700.045.0000041
GATCGGC253.890515E-545.012
GACCGTA253.890515E-545.09
CAACCCG207.032938E-445.023
AGGGCGT253.890515E-545.06
TCACGTA207.032938E-445.026
CGGACCC253.890515E-545.040
CGGTCCA302.1652759E-644.99999644
CGTTAGG2450.043.1632652
TAGGGCG4250.042.8823555
CTTTGCG1800.042.5000041
CGTTTAT5400.042.51
GTCTTCG750.042.01
TTAGGGA34700.041.952454
TCGAGGG6400.041.8359383
CGTTTTT12800.041.4843751
TAGGGAC15750.041.428575
ATCCGGG603.6379788E-1241.2499963
Produced by FastQC (version 0.11.5)