##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1027242
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.298014489282956	33.0	31.0	34.0	31.0	34.0
2	32.47714559957634	34.0	31.0	34.0	31.0	34.0
3	32.51867330190939	34.0	31.0	34.0	31.0	34.0
4	36.00461819123439	37.0	35.0	37.0	35.0	37.0
5	35.15569262160231	37.0	35.0	37.0	35.0	37.0
6	26.221189359469335	37.0	0.0	37.0	0.0	37.0
7	31.183695760103266	37.0	19.0	37.0	17.0	37.0
8	34.81011971862521	37.0	32.0	37.0	32.0	37.0
9	37.35281949141488	39.0	37.0	39.0	35.0	39.0
10	37.33972617942023	39.0	37.0	39.0	34.0	39.0
11	37.32205166844813	39.0	37.0	39.0	34.0	39.0
12	37.20648591081751	39.0	37.0	39.0	34.0	39.0
13	37.285869347242425	39.0	37.0	39.0	34.0	39.0
14	38.43906401802107	40.0	38.0	41.0	34.0	41.0
15	38.558485731697104	40.0	38.0	41.0	34.0	41.0
16	38.622096837940816	40.0	38.0	41.0	35.0	41.0
17	38.62578243490823	40.0	38.0	41.0	35.0	41.0
18	38.61805981453251	40.0	38.0	41.0	35.0	41.0
19	38.65227667871835	40.0	38.0	41.0	35.0	41.0
20	38.57759417936572	40.0	38.0	41.0	35.0	41.0
21	38.4983363219183	40.0	38.0	41.0	34.0	41.0
22	38.49214985368589	40.0	38.0	41.0	34.0	41.0
23	38.43015667194293	40.0	38.0	41.0	34.0	41.0
24	38.3691418380479	40.0	37.0	41.0	34.0	41.0
25	38.23247686523721	40.0	37.0	41.0	34.0	41.0
26	38.1794445709969	40.0	37.0	41.0	34.0	41.0
27	38.15789658133137	40.0	37.0	41.0	34.0	41.0
28	38.106258311089306	40.0	37.0	41.0	34.0	41.0
29	38.101351969642984	40.0	37.0	41.0	34.0	41.0
30	37.873854456885525	40.0	37.0	41.0	34.0	41.0
31	37.88943987882116	40.0	37.0	41.0	34.0	41.0
32	37.833228197445194	40.0	37.0	41.0	33.0	41.0
33	37.76578449868678	40.0	37.0	41.0	33.0	41.0
34	37.7308151341164	40.0	37.0	41.0	33.0	41.0
35	37.677415837748065	40.0	37.0	41.0	33.0	41.0
36	37.596180841515434	40.0	36.0	41.0	33.0	41.0
37	37.53858389746525	40.0	36.0	41.0	33.0	41.0
38	37.509209125016305	40.0	36.0	41.0	33.0	41.0
39	37.461528052786	40.0	36.0	41.0	33.0	41.0
40	37.416217405440975	40.0	36.0	41.0	33.0	41.0
41	37.34646461106536	40.0	36.0	41.0	33.0	41.0
42	37.286251925057584	40.0	36.0	41.0	33.0	41.0
43	37.223826517996734	39.0	36.0	41.0	32.0	41.0
44	37.120512985255665	39.0	35.0	41.0	32.0	41.0
45	37.099367043014205	39.0	35.0	41.0	32.0	41.0
46	37.01231550111853	39.0	35.0	41.0	32.0	41.0
47	36.940024843220975	39.0	35.0	41.0	31.0	41.0
48	36.938471168429636	39.0	35.0	41.0	32.0	41.0
49	36.94907529092463	39.0	35.0	41.0	32.0	41.0
50	36.88112635581489	39.0	35.0	41.0	31.0	41.0
51	35.91629820431797	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	3.0
12	1.0
13	8.0
14	6.0
15	13.0
16	18.0
17	43.0
18	92.0
19	195.0
20	395.0
21	713.0
22	1082.0
23	1804.0
24	2797.0
25	4145.0
26	5572.0
27	6546.0
28	7719.0
29	9497.0
30	12420.0
31	17410.0
32	24439.0
33	38413.0
34	56944.0
35	78853.0
36	100130.0
37	170192.0
38	241438.0
39	246210.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.974999075193576	4.320987654320987	60.227677606639915	14.476335663845521
2	19.51692006362668	3.7840158404738125	55.04116848804858	21.657895607850925
3	18.90907887333267	4.038191584845635	55.276361363729286	21.776368178092405
4	17.79541724345383	4.505072806602533	54.60923521429225	23.090274735651384
5	17.00572990590338	7.708212865128178	51.82722279657569	23.458834432392756
6	15.5285706776008	30.178088512736046	41.07522862188267	13.218112187780484
7	83.27326958983376	1.3317212497152568	10.402417346642757	4.992591813808236
8	85.35457078273669	1.3625805798438928	7.992079763093798	5.290768874325622
9	80.9964935234346	4.0241734664275794	9.960067832117456	5.019265178020369
10	37.86634502872741	35.38445663241963	15.22104820480471	11.528150134048257
11	22.534806793335942	25.683334598857915	31.67676165888856	20.105096948917588
12	22.08622700395817	23.02349397707648	34.11202034184739	20.778258677117954
13	21.90535433714743	24.94543642101861	34.58990189264068	18.559307349193276
14	18.79050895504662	27.92925133512843	32.112686202472254	21.1675535073527
15	16.20465284713826	27.53246070546181	34.56731714630048	21.69556930109945
16	19.11896125742522	28.233171930275436	31.139108408729392	21.508758403569946
17	19.269266638241035	27.039782251893907	29.55846820904908	24.132482900815972
18	19.528017740707643	26.488013535272113	32.671756022436774	21.312212701583462
19	20.597775402485492	27.569160918264636	30.719051596410583	21.11401208283929
20	21.459305596928473	29.589327539177724	29.910089346035306	19.0412775178585
21	21.81462595960835	28.860288033394273	30.384855759402363	18.940230247595018
22	21.070010766693727	26.35133688069608	29.648612498320748	22.930039854289447
23	19.216406650039623	27.92389719267709	28.5994926219917	24.260203535291584
24	20.307191489444552	27.294736780622287	29.620965653662918	22.777106076270247
25	18.54022713245759	29.682781661964757	27.364924720757134	24.412066484820517
26	18.083859499514233	31.52635893002817	27.289285290126376	23.100496280331217
27	18.912486054892614	30.410458295124226	29.176863874335357	21.500191775647803
28	17.2218425648484	28.47693143387829	30.109944881537164	24.191281119736146
29	17.611429439216856	26.871856875010952	29.513201368324115	26.00351231744808
30	20.430823506048235	28.80587047647974	27.926817634014185	22.83648838345784
31	21.050054417556915	31.07826588087325	24.669649410752285	23.20203029081755
32	18.915211800140572	32.05933947404799	23.430311455333797	25.595137270477647
33	19.558292982568858	30.86059565321511	24.086339927689874	25.494771436526154
34	18.219952065822852	29.438048677916207	26.000007787843565	26.341991468417376
35	16.538264595879063	30.41444956495159	24.740324091109983	28.30696174805937
36	18.743781893653104	32.96448159245825	24.61542654992689	23.676309963961753
37	18.798588842745918	31.565395495900674	27.09381041663016	22.54220524472325
38	19.03066658100039	28.91490028639795	25.41338847126578	26.64104466133589
39	18.231147090948387	28.166099127566824	26.79826175331616	26.80449202816863
40	19.726315707496383	28.431080504885898	26.541554959785525	25.301048827832197
41	17.439220748372826	30.063217820143645	24.713163986675	27.78439744480853
42	19.103969658561468	29.621160349752056	25.226869617869983	26.048000373816492
43	20.34856440838673	28.785232691030938	27.100235387571768	23.765967513010565
44	19.772361332577912	26.650876813837442	25.864304613713223	27.712457239871423
45	20.240702775003356	26.330407051113564	24.454023492030117	28.974866681852962
46	20.43646969263328	27.942198625056218	26.69020542384365	24.931126258466847
47	17.072705360567422	29.02558501307384	28.358750907770514	25.54295871858822
48	17.599066237556485	27.955437959117713	28.03185617410503	26.41363962922077
49	20.39879599938476	25.30533214179327	28.73753214919172	25.55833970963025
50	19.457927148617365	24.846822851869373	26.740437014841685	28.95481298467158
51	17.49597465835704	24.94076371487926	24.977171883548372	32.58608974321533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	346.5
2	578.0
3	3002.0
4	5426.0
5	3459.5
6	1493.0
7	1624.5
8	1756.0
9	2251.5
10	2747.0
11	3122.0
12	3497.0
13	3660.5
14	3824.0
15	3778.5
16	3733.0
17	3596.0
18	3459.0
19	3325.0
20	3191.0
21	3352.5
22	3514.0
23	3874.0
24	4234.0
25	5069.5
26	6862.0
27	7819.0
28	10038.5
29	12258.0
30	14063.0
31	15868.0
32	18839.0
33	21810.0
34	24943.0
35	28076.0
36	31306.0
37	34536.0
38	39062.5
39	43589.0
40	51832.5
41	60076.0
42	66911.5
43	73747.0
44	83982.5
45	94218.0
46	107841.5
47	121465.0
48	133596.0
49	145727.0
50	132830.5
51	119934.0
52	96442.5
53	72951.0
54	59618.0
55	46285.0
56	38051.0
57	29817.0
58	25427.0
59	21037.0
60	17262.5
61	13488.0
62	11078.5
63	8669.0
64	6961.5
65	5254.0
66	4055.0
67	2856.0
68	2318.5
69	1781.0
70	1460.5
71	1140.0
72	890.5
73	641.0
74	476.0
75	264.0
76	217.0
77	161.5
78	106.0
79	89.5
80	73.0
81	41.0
82	9.0
83	7.0
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1027242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.201719823259026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.71729238639864	28.11441202614347
2	12.522085405583988	9.817745673452487
3	4.670049226213401	5.49221883980536
4	2.3892969319268103	3.746581955998688
5	1.4856899921831195	2.912080140889127
6	1.0387807371256763	2.443319484875955
7	0.7711226270323148	2.116053322200739
8	0.6355791176032057	1.9932635595036057
9	0.5278025605417231	1.8621691290319808
>10	4.053765529342999	29.742311988873915
>50	0.1185383441984056	3.1835159126164907
>100	0.06309961328267501	4.403632657351117
>500	0.00306556825203533	0.7518983435391458
>1k	0.003831960315044163	3.4207969657179196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	4975	0.48430652173489785	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	4952	0.48206751670979187	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	4280	0.41664963075886696	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	3374	0.3284523023786021	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2843	0.2767604907120231	No Hit
TCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	1777	0.17298747520058563	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	1657	0.1613057098522062	No Hit
CTGTCACTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	1569	0.15273908193006128	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC	1421	0.13833157133372662	No Hit
CCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	1396	0.1358978702194809	No Hit
TCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	1388	0.1351190858629223	No Hit
TTCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	1272	0.12382671269282214	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.10834837360621938	No Hit
TGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTG	1089	0.10601202053654349	No Hit
GCTGTATCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	1052	0.10241014288745982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9469608913965744E-4	0.0	0.0	0.5116613222590198	0.0
2	1.9469608913965744E-4	0.0	0.0	1.8404621306371818	0.0
3	1.9469608913965744E-4	0.0	0.0	2.66568150445562	0.0
4	1.9469608913965744E-4	0.0	0.0	3.502874687756147	0.0
5	1.9469608913965744E-4	0.0	0.0	4.874411287700464	0.0
6	1.9469608913965744E-4	0.0	0.0	5.7382778352131245	0.0
7	1.9469608913965744E-4	0.0	0.0	6.841815268456703	0.0
8	1.9469608913965744E-4	0.0	0.0	8.573636981353955	0.0
9	1.9469608913965744E-4	0.0	0.0	9.346288411104686	0.0
10	1.9469608913965744E-4	0.0	0.0	10.721524236742656	0.0
11	1.9469608913965744E-4	0.0	0.0	13.768031291555447	0.0
12	1.9469608913965744E-4	0.0	0.0	16.31923149559695	0.0
13	1.9469608913965744E-4	0.0	0.0	17.124299824189432	0.0
14	1.9469608913965744E-4	0.0	0.0	17.394245951781567	0.0
15	1.9469608913965744E-4	0.0	0.0	17.72805239661151	0.0
16	1.9469608913965744E-4	0.0	0.0	18.587732978207665	0.0
17	1.9469608913965744E-4	0.0	0.0	20.051555524404183	0.0
18	1.9469608913965744E-4	0.0	0.0	21.52871475270676	0.0
19	1.9469608913965744E-4	0.0	0.0	22.62563251891959	0.0
20	1.9469608913965744E-4	0.0	0.0	23.683805763393632	0.0
21	1.9469608913965744E-4	0.0	0.0	25.17167327659889	0.0
22	1.9469608913965744E-4	0.0	0.0	26.782978110318698	0.0
23	1.9469608913965744E-4	0.0	0.0	28.4013893512921	0.0
24	1.9469608913965744E-4	0.0	0.0	29.580663563211004	0.0
25	1.9469608913965744E-4	0.0	0.0	30.576047318937505	0.0
26	1.9469608913965744E-4	0.0	0.0	31.432807459196567	0.0
27	1.9469608913965744E-4	0.0	0.0	32.29492174190697	0.0
28	1.9469608913965744E-4	0.0	0.0	33.20055060054009	0.0
29	1.9469608913965744E-4	0.0	0.0	34.130224426181954	0.0
30	1.9469608913965744E-4	0.0	0.0	35.197353690756415	0.0
31	1.9469608913965744E-4	0.0	0.0	36.1317975705822	0.0
32	1.9469608913965744E-4	0.0	0.0	36.9916728482675	0.0
33	1.9469608913965744E-4	0.0	0.0	37.82633498240921	0.0
34	1.9469608913965744E-4	0.0	0.0	38.6468816500883	0.0
35	1.9469608913965744E-4	0.0	0.0	39.56905967629828	0.0
36	1.9469608913965744E-4	0.0	0.0	40.430395174652126	0.0
37	1.9469608913965744E-4	0.0	0.0	41.266517529462384	0.0
38	1.9469608913965744E-4	0.0	0.0	42.09144485914712	0.0
39	1.9469608913965744E-4	0.0	0.0	42.979064329534815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGAGCG	45	3.8562575E-10	45.000004	1
GCCGATC	45	3.8562575E-10	45.000004	9
TCGCTAG	70	0.0	45.000004	1
GATCGGC	25	3.890515E-5	45.0	12
GACCGTA	25	3.890515E-5	45.0	9
CAACCCG	20	7.032938E-4	45.0	23
AGGGCGT	25	3.890515E-5	45.0	6
TCACGTA	20	7.032938E-4	45.0	26
CGGACCC	25	3.890515E-5	45.0	40
CGGTCCA	30	2.1652759E-6	44.999996	44
CGTTAGG	245	0.0	43.163265	2
TAGGGCG	425	0.0	42.882355	5
CTTTGCG	180	0.0	42.500004	1
CGTTTAT	540	0.0	42.5	1
GTCTTCG	75	0.0	42.0	1
TTAGGGA	3470	0.0	41.95245	4
TCGAGGG	640	0.0	41.835938	3
CGTTTTT	1280	0.0	41.484375	1
TAGGGAC	1575	0.0	41.42857	5
ATCCGGG	60	3.6379788E-12	41.249996	3
>>END_MODULE