FastQCFastQC Report
Sat 14 Jan 2017
SRR2934300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438342
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC233561.6238140859406176No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG197311.3717877945579007No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC155121.0784639536355052No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC58280.4051887520492345TruSeq Adapter, Index 13 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC53470.3717474703512795No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC50860.3536015773717238No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT50500.3510986955814403No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT42310.2941581348524899No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT40590.2821999218544685TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC38680.2689207434671309TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA37430.26023018169531303No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT36860.25626728552736416No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.2475767237555463No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC33370.23200323706044879TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC19570.13605943509957993TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT207.0338795E-445.029
CCGTTTA207.0338795E-445.020
AATCGCG207.0338795E-445.041
CGCGTAA253.891298E-544.99999613
TAGGGAT13950.042.2580645
TACGGCT28300.041.9787987
TTAGGGA26500.041.858494
CGTTTAT7000.041.7857131
TATTGCG650.041.538461
ATCCGGC1300.041.538466
TCGTTAG1850.041.3513531
ACGGCTG28950.041.113998
TAATACG1150.041.0869564
TTTAGGG50150.041.0518463
TAGGGAG15000.040.955
CGTTTTT16250.040.707691
GCGCTAG2050.040.609761
CTAGGGA16650.040.540544
CTTAGGG24800.040.4637073
GTTAGGG17200.040.4215133
Produced by FastQC (version 0.11.5)