##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934300.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1438342
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19592767227822	33.0	31.0	34.0	30.0	34.0
2	32.32953358797838	34.0	31.0	34.0	30.0	34.0
3	32.407979465245404	34.0	31.0	34.0	30.0	34.0
4	35.94959196074369	37.0	35.0	37.0	35.0	37.0
5	35.018385752484456	37.0	35.0	37.0	35.0	37.0
6	25.776690800936077	35.0	0.0	37.0	0.0	37.0
7	30.902164436552642	36.0	19.0	37.0	17.0	37.0
8	34.458308246578355	37.0	32.0	37.0	32.0	37.0
9	37.085079209256214	39.0	37.0	39.0	34.0	39.0
10	37.207780903290036	39.0	37.0	39.0	34.0	39.0
11	37.280541762668406	39.0	37.0	39.0	34.0	39.0
12	36.96276198567517	39.0	35.0	39.0	33.0	39.0
13	36.887683179661025	39.0	35.0	39.0	33.0	39.0
14	37.74054014970014	40.0	37.0	41.0	33.0	41.0
15	37.87034933277343	40.0	37.0	41.0	33.0	41.0
16	38.12291165800623	40.0	37.0	41.0	33.0	41.0
17	38.07557034418796	40.0	37.0	41.0	33.0	41.0
18	38.0877969217335	40.0	37.0	41.0	33.0	41.0
19	38.10275372616527	40.0	37.0	41.0	34.0	41.0
20	37.958752508096126	40.0	36.0	41.0	33.0	41.0
21	37.93380781483124	40.0	36.0	41.0	33.0	41.0
22	38.016544048633776	40.0	36.0	41.0	34.0	41.0
23	37.95156298015354	40.0	36.0	41.0	34.0	41.0
24	37.88007302852868	40.0	36.0	41.0	34.0	41.0
25	37.77455431323009	39.0	36.0	41.0	33.0	41.0
26	37.75920886687589	40.0	36.0	41.0	33.0	41.0
27	37.72280792746093	40.0	36.0	41.0	33.0	41.0
28	37.62830467301935	39.0	35.0	41.0	33.0	41.0
29	37.62609796557425	40.0	36.0	41.0	33.0	41.0
30	37.40130094233499	39.0	35.0	41.0	33.0	41.0
31	37.372516411256846	39.0	35.0	41.0	32.0	41.0
32	37.315446534968736	39.0	35.0	41.0	33.0	41.0
33	37.25477459463744	39.0	35.0	41.0	32.0	41.0
34	37.15816127179767	39.0	35.0	41.0	32.0	41.0
35	37.15986740288471	39.0	35.0	41.0	32.0	41.0
36	37.05197094988535	39.0	35.0	41.0	32.0	41.0
37	36.993459830833004	39.0	35.0	41.0	31.0	41.0
38	36.8480702086152	39.0	35.0	41.0	31.0	41.0
39	36.88324334546304	39.0	35.0	41.0	31.0	41.0
40	36.83084134371381	39.0	35.0	41.0	31.0	41.0
41	36.66251767660265	39.0	35.0	41.0	31.0	41.0
42	36.679664502600914	39.0	35.0	41.0	31.0	41.0
43	36.62188617171716	39.0	35.0	40.0	31.0	41.0
44	36.554515546372144	39.0	35.0	40.0	31.0	41.0
45	36.5109153455854	39.0	35.0	40.0	31.0	41.0
46	36.45381001180526	39.0	35.0	40.0	31.0	41.0
47	36.27291770663722	38.0	35.0	40.0	31.0	41.0
48	36.25198735766598	38.0	35.0	40.0	31.0	41.0
49	36.286109979406845	38.0	35.0	40.0	31.0	41.0
50	36.174915284403845	38.0	35.0	40.0	31.0	41.0
51	35.16125719752326	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	10.0
11	10.0
12	7.0
13	9.0
14	19.0
15	30.0
16	71.0
17	112.0
18	247.0
19	567.0
20	1081.0
21	1741.0
22	2869.0
23	4117.0
24	5591.0
25	7689.0
26	10034.0
27	12007.0
28	14119.0
29	17637.0
30	23191.0
31	32159.0
32	46402.0
33	73397.0
34	104460.0
35	116977.0
36	139130.0
37	226434.0
38	302216.0
39	295843.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.77522591984382	3.9711000582615257	51.14207886580521	14.111595156089443
2	19.548062978067804	11.686719848269744	47.831739600178544	20.933477573483913
3	19.217682581750374	11.809500104981986	48.12979110670481	20.843026206562833
4	18.244826334766	4.142199838425076	54.221735859760756	23.39123796704817
5	22.505774009241193	7.59673290496975	46.01367407751425	23.883819008274806
6	15.168784614507539	35.135037425035215	36.99509574218093	12.701082218276321
7	78.39289960245894	1.8008234481090033	15.04225003510987	4.764026914322185
8	78.53466004608084	6.786981121318853	9.101451532389376	5.576907300210937
9	74.00423543218511	4.004124192994434	10.457040119804608	11.534600255015844
10	41.70739643283725	23.9564721046872	18.617755721518247	15.718375740957297
11	32.4958181016754	21.518248093985992	25.816530421832915	20.169403382505692
12	27.45925517018901	19.248064785704653	30.80672051570489	22.48595952840145
13	26.098104623239816	21.014751707173954	34.95149276041442	17.93565090917181
14	21.440519709498854	24.993708033277205	32.6544729973817	20.911299259842234
15	16.99366353760093	22.564522206818687	39.48219547228684	20.959618783293543
16	22.456620191859795	24.130491913606082	28.54550586717206	24.86738202736206
17	22.79054633738012	23.977120879457043	30.110154608570145	23.12217817459269
18	23.701247686572458	22.024316887082488	31.62551048359848	22.64892494274658
19	23.14852795788484	24.87621163812223	28.181614664662508	23.793645739330422
20	22.56980606837595	28.617741816619414	30.565470520919224	18.246981594085412
21	22.928482933822416	28.03074651230375	30.76604868661278	18.274721867261054
22	20.83642137961625	21.62489866804974	32.999870684440836	24.53880926789317
23	20.34835943051096	26.50962010425893	30.943405671252037	22.198614793978066
24	22.186726105474218	24.425275768906143	28.49711681922658	24.890881306393055
25	21.15442641597061	29.386265575224808	25.237460909853148	24.221847098951432
26	19.59909395679192	24.001941123877355	27.72914925657458	28.669815662756143
27	21.924618762436193	24.597001269517264	29.42978790857807	24.048592059468472
28	16.545230550175134	24.054014970014084	32.1153800695523	27.285374410258477
29	20.703281973272002	23.096593160736457	28.743303053098636	27.4568218128929
30	23.91322786931064	23.4774483398246	30.003504034506395	22.60581975635836
31	23.12370771346453	22.45230967322097	29.59045901461544	24.83352359869906
32	24.416098535675104	24.437512079880864	28.192460485753735	22.953928898690297
33	23.015040929069723	22.283087054400134	25.635766736979104	29.066105279551042
34	19.76581369382247	21.769301042450266	30.74783326913905	27.717051994588214
35	19.734180048973055	23.650077658859995	29.228027826483547	27.387714465683406
36	22.235601824878923	25.407170200133212	26.505865781573508	25.851362193414364
37	21.32643001455843	24.020017492362733	31.804397007109575	22.84915548596926
38	19.9246771630113	23.8617102191273	30.961134417266546	25.25247820059485
39	19.567321262954152	22.747997346945304	29.822045104710842	27.86263628538971
40	21.097972526700882	20.990070511741994	30.298705036771505	27.61325192478562
41	19.069178262193553	24.115196524887683	26.775412245488205	30.040212967430556
42	19.538746695848413	22.21766450538189	33.88199746652743	24.361591332242263
43	20.682633198502167	24.038441483318987	29.22295253840881	26.05597277977004
44	21.926356874790557	20.980476131545906	29.366451094385067	27.726715899278474
45	20.25373659393941	19.43612854244679	27.807572886003467	32.502561977610334
46	23.258932854634015	21.706242326233955	28.749351684091824	26.285473135040206
47	16.92170568613028	22.260978265252632	35.573736983276575	25.24357906534051
48	18.81610910339822	24.62036149955991	29.95900835823469	26.60452103880718
49	20.930279446751886	19.8525802625523	34.58725393543399	24.629886355261824
50	20.578624555217047	20.74840336999128	29.706843017863623	28.966129056928047
51	18.730176828598484	20.248313683393796	27.29781929471572	33.723690193292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	678.5
2	1178.0
3	4471.0
4	7764.0
5	5013.5
6	2263.0
7	2262.5
8	2262.0
9	2458.5
10	2655.0
11	2738.5
12	2822.0
13	2774.5
14	2727.0
15	2746.0
16	2765.0
17	2704.5
18	2644.0
19	2919.0
20	3194.0
21	3103.5
22	3013.0
23	3608.5
24	4204.0
25	4937.0
26	6777.5
27	7885.0
28	9739.0
29	11593.0
30	14379.0
31	17165.0
32	20205.0
33	23245.0
34	26664.0
35	30083.0
36	32856.0
37	35629.0
38	39035.0
39	42441.0
40	48544.5
41	54648.0
42	62740.0
43	70832.0
44	82438.5
45	94045.0
46	151870.5
47	209696.0
48	191128.0
49	172560.0
50	166423.0
51	160286.0
52	138555.0
53	116824.0
54	103803.5
55	90783.0
56	81717.0
57	72651.0
58	65290.5
59	57930.0
60	49211.0
61	40492.0
62	35207.5
63	29923.0
64	25301.0
65	20679.0
66	17000.0
67	13321.0
68	10884.0
69	8447.0
70	7026.5
71	5606.0
72	4760.0
73	3914.0
74	3014.0
75	1735.0
76	1356.0
77	885.0
78	414.0
79	313.0
80	212.0
81	170.5
82	129.0
83	99.0
84	69.0
85	45.0
86	21.0
87	13.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1438342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.73283785175184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97366317860025	23.23169408569972
2	11.948263219695471	7.822011251606844
3	4.501700623147596	4.420603096638614
4	2.3393025678419517	3.0628804655742923
5	1.4617847502928223	2.392418160274925
6	1.077370368942058	2.115923375571746
7	0.8365149456753564	1.9167055654150906
8	0.6884098335345668	1.8026885965310757
9	0.5708620991155032	1.6817342873452608
>10	5.3882364647067655	34.877149989078006
>50	0.13754715214165306	3.019110252183438
>100	0.06652265967424156	3.8172311876188707
>500	0.004018146803888933	0.9061365766783693
>1k	0.0042413771818827625	3.0947183640076585
>5k	8.929215119753184E-4	1.5571870482697143
>10k+	6.696911339814888E-4	4.281807697506311
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC	23356	1.6238140859406176	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG	19731	1.3717877945579007	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	15512	1.0784639536355052	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	5828	0.4051887520492345	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	5347	0.3717474703512795	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTC	5086	0.3536015773717238	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	5050	0.3510986955814403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTAT	4231	0.2941581348524899	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	4059	0.2821999218544685	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	3868	0.2689207434671309	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTA	3743	0.26023018169531303	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	3686	0.25626728552736416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3561	0.2475767237555463	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	3337	0.23200323706044879	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	1957	0.13605943509957993	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.952449417454263E-5	0.0	0.0	0.2958267227126789	0.0
2	6.952449417454263E-5	0.0	0.0	1.2754268456319846	0.0
3	6.952449417454263E-5	0.0	0.0	1.7486800774780964	0.0
4	6.952449417454263E-5	0.0	0.0	2.5368097434407115	0.0
5	6.952449417454263E-5	0.0	0.0	4.5601115729082515	0.0
6	6.952449417454263E-5	0.0	0.0	5.248125967259525	0.0
7	6.952449417454263E-5	0.0	0.0	6.412244097718067	0.0
8	6.952449417454263E-5	0.0	0.0	8.001226412077239	0.0
9	6.952449417454263E-5	0.0	0.0	8.58648360403854	0.0
10	6.952449417454263E-5	0.0	0.0	11.170222381047067	0.0
11	6.952449417454263E-5	0.0	0.0	13.399316713271253	0.0
12	6.952449417454263E-5	0.0	0.0	17.066038536036633	0.0
13	6.952449417454263E-5	0.0	0.0	17.801329586426593	0.0
14	6.952449417454263E-5	0.0	0.0	18.1159279225664	0.0
15	6.952449417454263E-5	0.0	0.0	18.71940053200143	0.0
16	6.952449417454263E-5	0.0	0.0	19.500299650569893	0.0
17	6.952449417454263E-5	0.0	0.0	20.496724701079437	0.0
18	6.952449417454263E-5	0.0	0.0	21.51790047151512	0.0
19	6.952449417454263E-5	0.0	0.0	22.668461325609627	0.0
20	6.952449417454263E-5	0.0	0.0	23.53953371312247	0.0
21	6.952449417454263E-5	0.0	0.0	24.708449033679056	0.0
22	1.3904898834908526E-4	0.0	0.0	26.024200085932275	0.0
23	1.3904898834908526E-4	0.0	0.0	27.189569657285958	0.0
24	1.3904898834908526E-4	0.0	0.0	28.030816036797923	0.0
25	1.3904898834908526E-4	0.0	0.0	28.81866760478384	0.0
26	1.3904898834908526E-4	0.0	0.0	29.48763228773129	0.0
27	1.3904898834908526E-4	0.0	0.0	30.250176939837676	0.0
28	1.3904898834908526E-4	0.0	0.0	30.949593351233574	0.0
29	1.3904898834908526E-4	0.0	0.0	31.766923304749497	0.0
30	1.3904898834908526E-4	0.0	0.0	32.789211467091974	0.0
31	2.085734825236279E-4	0.0	0.0	33.536669303962476	0.0
32	2.085734825236279E-4	0.0	0.0	34.26445170898159	0.0
33	2.085734825236279E-4	0.0	0.0	34.96644052666195	0.0
34	2.085734825236279E-4	0.0	0.0	35.76388647484395	0.0
35	2.085734825236279E-4	0.0	0.0	36.64357989963444	0.0
36	2.085734825236279E-4	0.0	0.0	37.34341345799539	0.0
37	2.085734825236279E-4	0.0	0.0	38.07196063245042	0.0
38	2.085734825236279E-4	0.0	0.0	38.92106327980411	0.0
39	2.085734825236279E-4	0.0	0.0	40.46979091203622	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAT	20	7.0338795E-4	45.0	29
CCGTTTA	20	7.0338795E-4	45.0	20
AATCGCG	20	7.0338795E-4	45.0	41
CGCGTAA	25	3.891298E-5	44.999996	13
TAGGGAT	1395	0.0	42.258064	5
TACGGCT	2830	0.0	41.978798	7
TTAGGGA	2650	0.0	41.85849	4
CGTTTAT	700	0.0	41.785713	1
TATTGCG	65	0.0	41.53846	1
ATCCGGC	130	0.0	41.53846	6
TCGTTAG	185	0.0	41.351353	1
ACGGCTG	2895	0.0	41.11399	8
TAATACG	115	0.0	41.086956	4
TTTAGGG	5015	0.0	41.051846	3
TAGGGAG	1500	0.0	40.95	5
CGTTTTT	1625	0.0	40.70769	1
GCGCTAG	205	0.0	40.60976	1
CTAGGGA	1665	0.0	40.54054	4
CTTAGGG	2480	0.0	40.463707	3
GTTAGGG	1720	0.0	40.421513	3
>>END_MODULE