FastQCFastQC Report
Sat 14 Jan 2017
SRR2934297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742997
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT52860.7114429802542944TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC49600.6675666254372494TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC35030.4714689292150574TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC25550.3438775661274541TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT15640.21049883108545525TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.18506131249520522No Hit
CCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC13450.18102361113167348TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG13430.18075443104077135No Hit
TCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG11880.1598929739958573No Hit
TCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC11400.1534326518142065TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCC9210.12395743186042474No Hit
TTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT8640.11628579926971441No Hit
GCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC8370.11265186804253584TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAC207.031674E-445.010
CGTATAA207.031674E-445.026
CGTAGTG253.8894686E-545.035
CGGTACG207.031674E-445.032
GCGCTAA207.031674E-445.012
CCGTTAG253.8894686E-545.01
TACGTTG207.031674E-445.01
CCGATAA302.164461E-644.99999615
TCTCGTA600.044.99999628
CGATAAC302.164461E-644.99999616
GTAGGGA4300.042.383724
TAGGGAT9150.042.0491835
GCGTTAG750.042.0000041
TTAGGGA17750.041.9577454
TCGTTAG1400.041.7857131
GCGATGT1350.041.6666689
CTAGGGA9100.041.5384644
CGTTAGG2500.041.3999982
TAGGGAC6850.041.3868645
TCGTGTA1200.041.24999617
Produced by FastQC (version 0.11.5)