##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742997
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39351033718844	33.0	31.0	34.0	31.0	34.0
2	32.56608707706761	34.0	31.0	34.0	31.0	34.0
3	32.62448435188836	34.0	31.0	34.0	31.0	34.0
4	36.07873921429023	37.0	35.0	37.0	35.0	37.0
5	35.22316913796422	37.0	35.0	37.0	35.0	37.0
6	26.430848307597472	37.0	0.0	37.0	0.0	37.0
7	31.26855155538986	37.0	19.0	37.0	17.0	37.0
8	34.78433156526877	37.0	32.0	37.0	32.0	37.0
9	37.272137034200675	39.0	37.0	39.0	35.0	39.0
10	37.41450773017926	39.0	37.0	39.0	35.0	39.0
11	37.4916964671459	39.0	37.0	39.0	35.0	39.0
12	37.333227455830915	39.0	37.0	39.0	35.0	39.0
13	37.373725600507136	39.0	37.0	39.0	35.0	39.0
14	38.61067945092645	40.0	38.0	41.0	35.0	41.0
15	38.71140664094202	40.0	38.0	41.0	35.0	41.0
16	38.75685096978857	40.0	38.0	41.0	35.0	41.0
17	38.74197607796532	40.0	38.0	41.0	35.0	41.0
18	38.73132462176832	40.0	38.0	41.0	35.0	41.0
19	38.733145625083274	40.0	38.0	41.0	35.0	41.0
20	38.68909834090851	40.0	38.0	41.0	35.0	41.0
21	38.601352360776694	40.0	38.0	41.0	35.0	41.0
22	38.56389998882903	40.0	38.0	41.0	35.0	41.0
23	38.52328609671371	40.0	38.0	41.0	35.0	41.0
24	38.49492797413718	40.0	38.0	41.0	34.0	41.0
25	38.37923033336608	40.0	37.0	41.0	34.0	41.0
26	38.32865274018603	40.0	37.0	41.0	34.0	41.0
27	38.283602760172656	40.0	37.0	41.0	34.0	41.0
28	38.20529154222695	40.0	37.0	41.0	34.0	41.0
29	38.16099392056765	40.0	37.0	41.0	34.0	41.0
30	37.940434483584724	40.0	37.0	41.0	34.0	41.0
31	37.934734595159874	40.0	37.0	41.0	34.0	41.0
32	37.76637456140469	40.0	36.0	41.0	33.0	41.0
33	37.72148070584404	40.0	36.0	41.0	33.0	41.0
34	37.684779346349984	40.0	36.0	41.0	33.0	41.0
35	37.597921660518146	40.0	36.0	41.0	33.0	41.0
36	37.56077615387411	40.0	36.0	41.0	33.0	41.0
37	37.48185120532115	40.0	36.0	41.0	33.0	41.0
38	37.383229003616435	40.0	36.0	41.0	33.0	41.0
39	37.40665305512674	40.0	36.0	41.0	33.0	41.0
40	37.34265145081339	40.0	36.0	41.0	33.0	41.0
41	37.19794427164578	39.0	35.0	41.0	32.0	41.0
42	37.15601812658732	39.0	35.0	41.0	32.0	41.0
43	37.113170039717524	39.0	35.0	41.0	32.0	41.0
44	37.016744347554564	39.0	35.0	41.0	32.0	41.0
45	37.02467843073391	39.0	35.0	41.0	32.0	41.0
46	36.95214381753897	39.0	35.0	41.0	32.0	41.0
47	36.8589967388832	39.0	35.0	41.0	31.0	41.0
48	36.81132629068489	39.0	35.0	41.0	31.0	41.0
49	36.79006106350362	39.0	35.0	41.0	31.0	41.0
50	36.69855598340236	39.0	35.0	40.0	31.0	41.0
51	35.653481777180794	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	6.0
12	13.0
13	5.0
14	7.0
15	20.0
16	28.0
17	63.0
18	106.0
19	181.0
20	333.0
21	585.0
22	922.0
23	1406.0
24	2008.0
25	2850.0
26	3868.0
27	4732.0
28	5533.0
29	6777.0
30	8928.0
31	11951.0
32	16941.0
33	26808.0
34	41548.0
35	57604.0
36	71852.0
37	121698.0
38	174490.0
39	181583.0
40	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.589597266206997	4.358833211977975	54.131712510279314	17.91985701153571
2	20.227268750748657	4.607690206016982	53.01582644344459	22.149214599789772
3	21.091740612680805	4.480906383202086	51.962659337790065	22.46469366632705
4	19.759972112942584	4.578214986063201	51.129546956448	24.532265944546207
5	18.263465397572265	7.858174393705493	48.960897554095105	24.917462654627137
6	16.45470977675549	30.408871099075768	39.24066988157422	13.895749242594519
7	81.29211827234835	2.1152171543088327	10.89964024080851	5.693024332534317
8	81.26708452389444	2.878746482152687	9.551451755525257	6.302717238427611
9	75.27675078095874	5.368393142906364	12.350924700907271	7.003931375227626
10	36.283591992969015	29.312365998786	19.424439129633093	14.979602878611892
11	25.709928842242967	25.589336161518823	28.559873054669133	20.140861941569078
12	24.693370228951125	22.146926569017104	33.14629803350485	20.013405168526926
13	23.425801180893057	23.55312336388976	32.64912240560864	20.371953049608546
14	18.50599665947507	26.975344449573818	31.421392011004084	23.097266879947025
15	17.647581349588222	26.484225373722907	34.681028321783266	21.187164954905604
16	19.499540374994783	25.43657645993187	31.61708593708992	23.446797227983424
17	19.088367786141802	25.915986201828538	29.672394370367577	25.323251641662083
18	20.302369996110347	24.790544241766792	31.344541095051525	23.562544667071332
19	21.466304709171098	25.9359055285553	30.089219741129508	22.508570021144095
20	24.738996254359037	25.357706693297548	30.271050892533886	19.632246159809526
21	24.69794629049646	25.74196127306032	29.53632383441656	20.023768602026657
22	21.66940108775675	25.269550213527108	28.748702888436966	24.31234581027918
23	21.43198424758108	25.83900069583053	28.53201291526076	24.197002141327623
24	22.687843961684905	24.410192773322102	28.932956660659464	23.969006604333533
25	20.797661363370242	25.931733237146315	27.90415035323157	25.36645504625187
26	19.461316802086685	28.210208116587282	29.19325380856181	23.135221272764223
27	21.18312725354207	28.17319585408824	28.800250875844718	21.843426016524965
28	19.926056229029186	27.576154412467346	30.03861388403991	22.459175474463557
29	21.03669328409132	27.04398537275386	29.303752235877127	22.61556910727769
30	24.47789156618398	27.020297524754472	27.343044453746113	21.15876645531543
31	24.83388223640203	27.937932454639792	26.06645787264282	21.161727436315356
32	23.86362259874535	27.963773743366392	27.20953112865866	20.963072529229592
33	25.458918407476745	26.786918385942336	26.63846556580982	21.115697640771096
34	23.9773511871515	24.708444314041643	28.69446310012019	22.61974139868667
35	22.550696705370278	24.827960274402187	29.020440190202653	23.600902830024886
36	25.09700577525885	26.465651947450663	27.885576926959327	20.55176535033116
37	24.22930375223588	28.230800393541294	28.037798268364476	19.502097585858355
38	23.28596212366941	28.127973598816684	26.935505796120307	21.6505584813936
39	25.414369102432445	25.594854353382313	27.969022755138983	21.021753789046254
40	26.109795867278063	23.52983928602673	29.43215113923744	20.92821370745777
41	21.269668652767105	24.920827405763415	29.190292827561887	24.619211113907593
42	23.8126129715194	24.367931499050467	28.555162403078345	23.26429312635179
43	24.86685679753754	24.318133182233577	28.15650668845231	22.658503331776576
44	22.4519076120092	24.761607381994814	27.816128463506583	24.970356542489405
45	23.36240926948561	23.463620983664807	27.18476656029567	25.989203186553915
46	23.644375414705575	24.155279227237795	29.731613990366046	22.468731367690584
47	21.64059881803022	24.613019971816843	31.444810678912567	22.30157053124037
48	21.485685675716052	23.36442812016738	31.13081210287525	24.019074101241326
49	23.206688586898736	22.648543668413197	31.76580793731334	22.378959807374727
50	23.04746856313013	22.574653733460565	29.509002055189992	24.86887564821931
51	21.47787945307989	22.283266285059025	28.088942485635876	28.149911776225206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	510.5
2	805.0
3	2111.5
4	3418.0
5	2343.5
6	1269.0
7	1308.5
8	1348.0
9	1542.0
10	1736.0
11	1834.5
12	1933.0
13	1960.5
14	1988.0
15	2033.0
16	2078.0
17	2114.5
18	2151.0
19	2272.0
20	2393.0
21	2525.5
22	2658.0
23	3020.0
24	3382.0
25	4306.0
26	6408.5
27	7587.0
28	8213.5
29	8840.0
30	10363.0
31	11886.0
32	13786.0
33	15686.0
34	18126.5
35	20567.0
36	21953.5
37	23340.0
38	25782.5
39	28225.0
40	31806.5
41	35388.0
42	39820.5
43	44253.0
44	49870.5
45	55488.0
46	64211.5
47	72935.0
48	82843.5
49	92752.0
50	84677.5
51	76603.0
52	62804.0
53	49005.0
54	42979.0
55	36953.0
56	34059.5
57	31166.0
58	29943.0
59	28720.0
60	26160.5
61	23601.0
62	20515.5
63	17430.0
64	14631.5
65	11833.0
66	9890.0
67	7947.0
68	6519.0
69	5091.0
70	3990.5
71	2890.0
72	2447.5
73	2005.0
74	1537.0
75	817.5
76	566.0
77	448.0
78	330.0
79	233.5
80	137.0
81	102.5
82	68.0
83	42.5
84	17.0
85	12.5
86	8.0
87	6.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.92765920788209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48369955772156	27.491370549517768
2	9.9747130311835	7.566350330862967
3	3.781294295057929	4.302469241629976
4	2.2939591823373613	3.480180084179332
5	1.6594380505520832	3.146930042896581
6	1.3005445649986391	2.959596662755898
7	1.099817872989373	2.9199462234235076
8	0.9223523930502964	2.798613378654892
9	0.8240575136370866	2.8129115290429856
>10	5.531750226501197	33.112805611809975
>50	0.08206856996909925	2.1333203435424464
>100	0.03839905434297433	2.748572083846715
>500	0.004141074487967805	1.0631619603284468
>1k	0.0033881518537918404	2.709021202432396
>5k	3.7646131708798225E-4	0.7547507550760368
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	5286	0.7114429802542944	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	4960	0.6675666254372494	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	3503	0.4714689292150574	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	2555	0.3438775661274541	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGCT	1564	0.21049883108545525	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.18506131249520522	No Hit
CCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	1345	0.18102361113167348	TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	1343	0.18075443104077135	No Hit
TCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTG	1188	0.1598929739958573	No Hit
TCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	1140	0.1534326518142065	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCC	921	0.12395743186042474	No Hit
TTCCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCT	864	0.11628579926971441	No Hit
GCTGTATCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	837	0.11265186804253584	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3459004545105836E-4	0.0	0.0	0.7628563776165987	0.0
2	1.3459004545105836E-4	0.0	0.0	2.403239851574098	0.0
3	1.3459004545105836E-4	0.0	0.0	3.2853430094603344	0.0
4	1.3459004545105836E-4	0.0	0.0	4.182520252437089	0.0
5	1.3459004545105836E-4	0.0	0.0	5.530843327765791	0.0
6	1.3459004545105836E-4	0.0	0.0	6.319271814018092	0.0
7	1.3459004545105836E-4	0.0	0.0	7.277014577447823	0.0
8	1.3459004545105836E-4	0.0	0.0	8.608379307049692	0.0
9	1.3459004545105836E-4	0.0	0.0	9.242298421124177	0.0
10	1.3459004545105836E-4	0.0	0.0	10.443783756865775	0.0
11	1.3459004545105836E-4	0.0	0.0	12.50476112285783	0.0
12	1.3459004545105836E-4	0.0	0.0	14.265198917357674	0.0
13	1.3459004545105836E-4	0.0	0.0	14.897502950886746	0.0
14	1.3459004545105836E-4	0.0	0.0	15.148378795607519	0.0
15	1.3459004545105836E-4	0.0	0.0	15.527788133734052	0.0
16	1.3459004545105836E-4	0.0	0.0	16.39212540562075	0.0
17	1.3459004545105836E-4	0.0	0.0	17.581766817362656	0.0
18	1.3459004545105836E-4	0.0	0.0	18.82753227805765	0.0
19	1.3459004545105836E-4	0.0	0.0	19.68204447662642	0.0
20	2.691800909021167E-4	0.0	0.0	20.499006052514343	0.0
21	2.691800909021167E-4	0.0	0.0	21.51314204498807	0.0
22	2.691800909021167E-4	0.0	0.0	22.524855416643675	0.0
23	2.691800909021167E-4	0.0	0.0	23.47425359725544	0.0
24	2.691800909021167E-4	0.0	0.0	24.23872505541745	0.0
25	2.691800909021167E-4	0.0	0.0	24.89081382562783	0.0
26	2.691800909021167E-4	0.0	0.0	25.45595742647682	0.0
27	2.691800909021167E-4	0.0	0.0	26.024869548598446	0.0
28	2.691800909021167E-4	0.0	0.0	26.624737381173812	0.0
29	2.691800909021167E-4	0.0	0.0	27.232546026430793	0.0
30	2.691800909021167E-4	0.0	0.0	27.8823467658685	0.0
31	2.691800909021167E-4	0.0	0.0	28.50280687539788	0.0
32	2.691800909021167E-4	0.0	0.0	29.09002324370085	0.0
33	2.691800909021167E-4	0.0	0.0	29.662030936867847	0.0
34	2.691800909021167E-4	0.0	0.0	30.229731748580413	0.0
35	2.691800909021167E-4	0.0	0.0	30.790703058020423	0.0
36	2.691800909021167E-4	0.0	0.0	31.341849294142506	0.0
37	2.691800909021167E-4	0.0	0.0	31.90981928594597	0.0
38	2.691800909021167E-4	0.0	0.0	32.445218486750285	0.0
39	2.691800909021167E-4	0.0	0.0	32.98909686041801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAC	20	7.031674E-4	45.0	10
CGTATAA	20	7.031674E-4	45.0	26
CGTAGTG	25	3.8894686E-5	45.0	35
CGGTACG	20	7.031674E-4	45.0	32
GCGCTAA	20	7.031674E-4	45.0	12
CCGTTAG	25	3.8894686E-5	45.0	1
TACGTTG	20	7.031674E-4	45.0	1
CCGATAA	30	2.164461E-6	44.999996	15
TCTCGTA	60	0.0	44.999996	28
CGATAAC	30	2.164461E-6	44.999996	16
GTAGGGA	430	0.0	42.38372	4
TAGGGAT	915	0.0	42.049183	5
GCGTTAG	75	0.0	42.000004	1
TTAGGGA	1775	0.0	41.957745	4
TCGTTAG	140	0.0	41.785713	1
GCGATGT	135	0.0	41.666668	9
CTAGGGA	910	0.0	41.538464	4
CGTTAGG	250	0.0	41.399998	2
TAGGGAC	685	0.0	41.386864	5
TCGTGTA	120	0.0	41.249996	17
>>END_MODULE