FastQCFastQC Report
Sat 14 Jan 2017
SRR2934296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289978
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT49300.38217706038397553TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC49080.38047160494209975TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC46010.3566727494577427TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC42470.32923042098392374TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.16535165716004457No Hit
TCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG19330.14984751677935593No Hit
TCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC17670.13697908026338435TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC17540.13597131113863958TruSeq Adapter, Index 20 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT13490.10457542686774504No Hit
CTGTCACTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT13470.10442038546393814TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC13170.10209476440683485TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1600.045.0000041
AGTCCGC207.033607E-445.00000411
GCGACTC406.8157533E-945.0000049
TATTGCG453.8562575E-1045.0000041
GTGTCGT207.033607E-445.00000414
GCGATCG207.033607E-445.0000049
GCGATAT502.1827873E-1145.0000049
TCCGTAG253.8910708E-545.0000041
TCGCGCG406.8157533E-945.0000041
TCGCGAG253.8910708E-545.0000041
GCATCCG253.8910708E-545.0000041
GTCGGTC207.033607E-445.00000410
CGGACGG502.1827873E-1145.0000042
CGCGCGG700.045.02
CGTAAGG302.1657088E-644.9999962
ACGAAGC302.1657088E-644.99999611
TAGGGCG6850.044.3430635
CGTTTAT8000.042.7500041
GCGCTAG700.041.7857131
TTAGGGT19000.041.4473724
Produced by FastQC (version 0.11.5)