##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1289978
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22081306812984	33.0	31.0	34.0	30.0	34.0
2	32.37791109615823	34.0	31.0	34.0	30.0	34.0
3	32.42931274796935	34.0	31.0	34.0	30.0	34.0
4	35.947592129478174	37.0	35.0	37.0	35.0	37.0
5	35.055390091924046	37.0	35.0	37.0	35.0	37.0
6	25.707324465998646	35.0	0.0	37.0	0.0	37.0
7	30.909210854758765	37.0	19.0	37.0	17.0	37.0
8	34.69989100589312	37.0	32.0	37.0	32.0	37.0
9	37.27115423673892	39.0	37.0	39.0	35.0	39.0
10	37.1296122879615	39.0	37.0	39.0	34.0	39.0
11	37.209409772879845	39.0	37.0	39.0	34.0	39.0
12	37.109375508729606	39.0	37.0	39.0	34.0	39.0
13	37.19315135606964	39.0	37.0	39.0	34.0	39.0
14	38.38593991525437	40.0	38.0	41.0	34.0	41.0
15	38.48611526708208	40.0	38.0	41.0	34.0	41.0
16	38.542186765975856	40.0	38.0	41.0	34.0	41.0
17	38.541777456669806	40.0	38.0	41.0	34.0	41.0
18	38.53358661930669	40.0	38.0	41.0	34.0	41.0
19	38.53437035360293	40.0	38.0	41.0	34.0	41.0
20	38.4689172993648	40.0	38.0	41.0	34.0	41.0
21	38.371654400307605	40.0	38.0	41.0	34.0	41.0
22	38.386015110335215	40.0	37.0	41.0	34.0	41.0
23	38.320676786735895	40.0	37.0	41.0	34.0	41.0
24	38.28031795891093	40.0	37.0	41.0	34.0	41.0
25	38.17209983426074	40.0	37.0	41.0	34.0	41.0
26	38.124980426022766	40.0	37.0	41.0	34.0	41.0
27	38.087827079221505	40.0	37.0	41.0	34.0	41.0
28	38.06109018913501	40.0	37.0	41.0	34.0	41.0
29	38.052091586058054	40.0	37.0	41.0	34.0	41.0
30	37.77297054678452	40.0	37.0	41.0	33.0	41.0
31	37.843913617131456	40.0	37.0	41.0	33.0	41.0
32	37.79214994364245	40.0	37.0	41.0	33.0	41.0
33	37.69427230541916	40.0	37.0	41.0	33.0	41.0
34	37.65609180931768	40.0	37.0	41.0	33.0	41.0
35	37.610523590324796	40.0	37.0	41.0	33.0	41.0
36	37.48721838667016	40.0	37.0	41.0	33.0	41.0
37	37.431630616956255	40.0	37.0	41.0	33.0	41.0
38	37.377054492402195	40.0	36.0	41.0	33.0	41.0
39	37.32701565453054	40.0	36.0	41.0	32.0	41.0
40	37.29845857836335	40.0	36.0	41.0	32.0	41.0
41	37.242962283077695	40.0	36.0	41.0	32.0	41.0
42	37.17819528705141	40.0	36.0	41.0	32.0	41.0
43	37.08037501414753	39.0	36.0	41.0	32.0	41.0
44	36.93159030619127	39.0	36.0	41.0	31.0	41.0
45	36.901036296743044	39.0	35.0	41.0	31.0	41.0
46	36.80817269751887	39.0	35.0	41.0	31.0	41.0
47	36.738379259181166	39.0	35.0	41.0	31.0	41.0
48	36.70012666882691	39.0	35.0	41.0	31.0	41.0
49	36.64650482411328	39.0	35.0	40.0	31.0	41.0
50	36.52195308757204	39.0	35.0	40.0	31.0	41.0
51	35.45103249822865	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	3.0
11	7.0
12	5.0
13	1.0
14	5.0
15	12.0
16	27.0
17	59.0
18	128.0
19	290.0
20	544.0
21	993.0
22	1492.0
23	2584.0
24	4035.0
25	6506.0
26	8501.0
27	9937.0
28	11287.0
29	13540.0
30	17172.0
31	23177.0
32	32150.0
33	49120.0
34	72483.0
35	98476.0
36	127911.0
37	219669.0
38	298954.0
39	290769.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.17599369911735	4.3434849276499286	58.54720002976796	12.93332134346477
2	20.490426968521945	4.081542475918194	53.99068821328736	21.4373423422725
3	19.79398098262141	4.265189018727451	53.909911641903975	22.03091835674717
4	17.918755203577117	4.069449246421257	53.533548634162756	24.478246915838874
5	16.563615813603022	7.604160691112562	52.89694863013168	22.93527486515274
6	14.261018404965045	31.75922380071598	41.728618627604504	12.251139166714472
7	83.43545393797413	1.463978455446527	10.501109321244238	4.599458285335099
8	85.31199756895079	1.4565364680637964	8.002229495386743	5.229236467598672
9	80.91812418506362	3.3262582772729457	10.481651625066474	5.273965912596959
10	37.10187305519939	19.8635170522288	24.54227901561112	18.49233087696069
11	26.197811125461058	27.42093276009358	24.58165953217807	21.799596582267295
12	20.823610945302942	23.411174454137978	33.83173976610454	21.933474834454543
13	21.888977951561962	26.106259176513085	34.03879756088863	17.96596531103631
14	20.67601152887879	27.13464880796417	32.96242261495932	19.226917048197723
15	18.48620674151032	27.789001052731134	34.26283238938959	19.461959816368964
16	18.859313879771594	25.15709570240733	32.560322734186165	23.423267683634915
17	18.526129902990593	26.57843777180696	29.73050703190287	25.16492529329958
18	20.15763059525046	25.582296752347716	32.8650566133686	21.395016039033223
19	21.079584302988113	28.511804077278835	29.868571401992906	20.540040217740145
20	21.677423956067468	27.56628407616254	31.055878472346038	19.700413495423952
21	21.441605980877192	28.598937346218307	30.40974342198084	19.54971325092366
22	20.30546257378033	25.47795388758568	29.34189575326091	24.874687785373084
23	18.7176060366921	27.519539092914762	28.534750205042258	25.22810466535088
24	20.293679427091003	26.028350871100127	29.993379732057445	23.68458996975142
25	19.47560345990397	27.147904847989658	28.259164109775515	25.117327582330862
26	19.33474834454541	28.459787686301624	29.818725590668986	22.386738378483976
27	19.88809111473219	28.526920614150008	29.465618793498805	22.119369477618996
28	17.690689298577187	26.160213585037884	31.046188384608108	25.102908731776818
29	19.23032795908147	24.64406369721034	29.88787405676686	26.23773428694133
30	19.739483929183287	28.821111677873578	28.269784445936285	23.169619947006847
31	18.413104719615372	28.70731128747932	28.15458868290777	24.724995309997535
32	18.768692179246468	28.479788027392715	28.56141732649704	24.190102466863774
33	19.778321800836913	28.375522683332584	27.558144402462677	24.288011113367823
34	18.22705503504711	26.275331827364496	29.653528974912753	25.84408416267564
35	19.591496909249614	26.13416662919833	28.45118288839035	25.82315357316171
36	21.470366161283373	28.448934788035146	27.434266320820978	22.64643272986051
37	17.577896677307674	27.97667867203937	29.873377685510917	24.572046965142043
38	17.08494253390368	28.20272903878981	28.347227627137826	26.365100800168683
39	19.76762394397424	26.612546880644476	29.372981554724188	24.246847620657096
40	20.184142675301437	25.38818491478149	31.557592455065127	22.870079954851942
41	19.71475482527609	25.121591220935553	30.990450999939533	24.173202953848826
42	22.340380998745715	24.836237517228977	29.126620764075046	23.696760719950262
43	21.86432636835667	24.69925843696559	28.35156878644442	25.084846408233318
44	19.42568012787815	24.891122174176612	26.959219459556678	28.723978238388558
45	20.49686118677993	24.59476053079975	26.821930296485675	28.086447985934644
46	21.252998113146116	25.25911294611226	29.29329027316745	24.194598667574176
47	18.847608253784173	25.52407870521823	31.332162253929912	24.296150787067685
48	18.878694055247454	24.388943067246107	30.321214780407107	26.41114809709933
49	20.66453846499708	22.84519580954094	31.083243280117955	25.40702244534403
50	19.978480253151602	22.18200620475698	29.75190274562822	28.087610796463196
51	18.415972985585803	22.63588991440164	28.087610796463196	30.860526303549364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	329.5
2	590.0
3	2975.5
4	5361.0
5	3808.0
6	2255.0
7	2290.0
8	2325.0
9	2528.5
10	2732.0
11	2799.5
12	2867.0
13	3011.5
14	3156.0
15	3131.0
16	3106.0
17	3090.5
18	3075.0
19	3061.0
20	3047.0
21	3270.5
22	3494.0
23	4110.0
24	4726.0
25	5654.0
26	7781.5
27	8981.0
28	11084.5
29	13188.0
30	16344.5
31	19501.0
32	24148.5
33	28796.0
34	31156.0
35	33516.0
36	38196.0
37	42876.0
38	47932.0
39	52988.0
40	62197.5
41	71407.0
42	81013.5
43	90620.0
44	102952.0
45	115284.0
46	134801.5
47	154319.0
48	170610.0
49	186901.0
50	172290.0
51	157679.0
52	129453.5
53	101228.0
54	81521.0
55	61814.0
56	51146.5
57	40479.0
58	33346.5
59	26214.0
60	21548.5
61	16883.0
62	13884.0
63	10885.0
64	8380.5
65	5876.0
66	4634.0
67	3392.0
68	2574.0
69	1756.0
70	1386.0
71	1016.0
72	715.0
73	414.0
74	342.5
75	223.0
76	175.0
77	123.0
78	71.0
79	43.0
80	15.0
81	19.0
82	23.0
83	18.0
84	13.0
85	8.0
86	3.0
87	4.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1289978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.0058314051805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39854670517596	27.459538439518187
2	13.226842296204532	10.31847961257329
3	5.124291897976303	5.9963179753018805
4	2.5692346541994238	4.008605350481997
5	1.5683491051961707	3.058738039087376
6	1.0061116300778732	2.354653237056531
7	0.7874900471088966	2.150169280755109
8	0.6355676802523377	1.9832676626003483
9	0.4984337432959385	1.7497640301888953
>10	3.9855698527871777	29.389317909331147
>50	0.12754138261973202	3.4004434383868998
>100	0.06574010449060891	4.503602159081557
>500	0.0031404503075485105	0.7920583292538912
>1k	0.0031404503075485105	2.835044536382935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	4930	0.38217706038397553	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	4908	0.38047160494209975	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	4601	0.3566727494577427	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	4247	0.32923042098392374	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	0.16535165716004457	No Hit
TCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTG	1933	0.14984751677935593	No Hit
TCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1767	0.13697908026338435	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1754	0.13597131113863958	TruSeq Adapter, Index 20 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCT	1349	0.10457542686774504	No Hit
CTGTCACTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	1347	0.10442038546393814	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1317	0.10209476440683485	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5504140380688663E-4	0.0	0.0	0.4037278155131328	0.0
2	1.5504140380688663E-4	0.0	0.0	1.582895212166409	0.0
3	1.5504140380688663E-4	0.0	0.0	2.274844997356544	0.0
4	1.5504140380688663E-4	0.0	0.0	3.056486234648963	0.0
5	1.5504140380688663E-4	0.0	0.0	4.325422604106427	0.0
6	1.5504140380688663E-4	0.0	0.0	5.175282059073876	0.0
7	1.5504140380688663E-4	0.0	0.0	6.180648042059632	0.0
8	1.5504140380688663E-4	0.0	0.0	7.860056528095828	0.0
9	1.5504140380688663E-4	0.0	0.0	8.507741992499097	0.0
10	1.5504140380688663E-4	0.0	0.0	10.109164652420429	0.0
11	1.5504140380688663E-4	0.0	0.0	12.939290437511337	0.0
12	1.5504140380688663E-4	0.0	0.0	15.204522867831855	0.0
13	1.5504140380688663E-4	0.0	0.0	15.967791698773157	0.0
14	1.5504140380688663E-4	0.0	0.0	16.188183054284647	0.0
15	1.5504140380688663E-4	0.0	0.0	16.54237514128148	0.0
16	1.5504140380688663E-4	0.0	0.0	17.556035839370903	0.0
17	1.5504140380688663E-4	0.0	0.0	19.206684145000924	0.0
18	1.5504140380688663E-4	0.0	0.0	20.929426703401145	0.0
19	1.5504140380688663E-4	0.0	0.0	22.049755887309708	0.0
20	1.5504140380688663E-4	0.0	0.0	23.115743059183956	0.0
21	1.5504140380688663E-4	0.0	0.0	24.757011359883656	0.0
22	1.5504140380688663E-4	0.0	0.0	26.553786188601666	0.0
23	1.5504140380688663E-4	0.0	0.0	28.470330501760497	0.0
24	1.5504140380688663E-4	0.0	0.0	29.800895829231195	0.0
25	1.5504140380688663E-4	0.0	0.0	31.01967630455713	0.0
26	1.5504140380688663E-4	0.0	0.0	32.0590738756785	0.0
27	1.5504140380688663E-4	0.0	0.0	33.05909093023292	0.0
28	1.5504140380688663E-4	0.0	0.0	34.16709432253884	0.0
29	1.5504140380688663E-4	0.0	0.0	35.20656941436211	0.0
30	1.5504140380688663E-4	0.0	0.0	36.30465015682438	0.0
31	1.5504140380688663E-4	0.0	0.0	37.410482969477	0.0
32	1.5504140380688663E-4	0.0	0.0	38.43724466618811	0.0
33	1.5504140380688663E-4	0.0	0.0	39.417493941757144	0.0
34	1.5504140380688663E-4	0.0	0.0	40.361153446027764	0.0
35	1.5504140380688663E-4	0.0	0.0	41.316363534881994	0.0
36	1.5504140380688663E-4	0.0	0.0	42.26095328757545	0.0
37	1.5504140380688663E-4	0.0	0.0	43.25562141369853	0.0
38	1.5504140380688663E-4	0.0	0.0	44.222537128540175	0.0
39	1.5504140380688663E-4	0.0	0.0	45.18069300406673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	160	0.0	45.000004	1
AGTCCGC	20	7.033607E-4	45.000004	11
GCGACTC	40	6.8157533E-9	45.000004	9
TATTGCG	45	3.8562575E-10	45.000004	1
GTGTCGT	20	7.033607E-4	45.000004	14
GCGATCG	20	7.033607E-4	45.000004	9
GCGATAT	50	2.1827873E-11	45.000004	9
TCCGTAG	25	3.8910708E-5	45.000004	1
TCGCGCG	40	6.8157533E-9	45.000004	1
TCGCGAG	25	3.8910708E-5	45.000004	1
GCATCCG	25	3.8910708E-5	45.000004	1
GTCGGTC	20	7.033607E-4	45.000004	10
CGGACGG	50	2.1827873E-11	45.000004	2
CGCGCGG	70	0.0	45.0	2
CGTAAGG	30	2.1657088E-6	44.999996	2
ACGAAGC	30	2.1657088E-6	44.999996	11
TAGGGCG	685	0.0	44.343063	5
CGTTTAT	800	0.0	42.750004	1
GCGCTAG	70	0.0	41.785713	1
TTAGGGT	1900	0.0	41.447372	4
>>END_MODULE