FastQCFastQC Report
Sat 14 Jan 2017
SRR2934295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211825
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT28830.23790563819033275Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC28630.23625523487302208Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC21460.17708827594743465Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC20470.16891877952674683Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.1687537391950158No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC207.0334383E-445.019
TATTCGC207.0334383E-445.032
CGGTCTA253.890931E-545.031
GCCGATT2050.043.9024439
CGTTAGG3300.041.5909082
TAGGGCG6050.041.2809945
TCGTTAG1800.041.2500041
TTAGGGT12800.040.957034
CGAGGGT1650.040.909094
AGGGCGA7850.040.7006346
GTTAGGG17650.040.665723
TTTAGGG48150.040.467293
TTAGGGA24300.040.277784
CGTTTAT5200.040.2403831
TGTAGGG16350.040.1834833
CTTAGGG22000.039.681823
CTCGTAG800.039.3751
ATTACGA403.4584264E-739.37529
TTGAGGG20350.039.3611763
CGTTTTT14250.039.1578941
Produced by FastQC (version 0.11.5)