##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211825
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38847275803025	33.0	31.0	34.0	31.0	34.0
2	32.55603490603016	34.0	31.0	34.0	31.0	34.0
3	32.60521362407938	34.0	31.0	34.0	31.0	34.0
4	36.06508695562478	37.0	35.0	37.0	35.0	37.0
5	35.2096721886411	37.0	35.0	37.0	35.0	37.0
6	26.29923875146989	37.0	0.0	37.0	0.0	37.0
7	31.224483733212303	37.0	19.0	37.0	17.0	37.0
8	34.83287190807253	37.0	32.0	37.0	32.0	37.0
9	37.33930064159429	39.0	37.0	39.0	35.0	39.0
10	37.36177088275947	39.0	37.0	39.0	34.0	39.0
11	37.420930414870135	39.0	37.0	39.0	35.0	39.0
12	37.235723805004845	39.0	37.0	39.0	34.0	39.0
13	37.29067150784973	39.0	37.0	39.0	34.0	39.0
14	38.50792977533885	40.0	38.0	41.0	34.0	41.0
15	38.63778185794153	40.0	38.0	41.0	35.0	41.0
16	38.67493078621088	40.0	38.0	41.0	35.0	41.0
17	38.665555670166896	40.0	38.0	41.0	35.0	41.0
18	38.634523961793164	40.0	38.0	41.0	35.0	41.0
19	38.64386441936748	40.0	38.0	41.0	35.0	41.0
20	38.585445093144635	40.0	38.0	41.0	35.0	41.0
21	38.49500546696099	40.0	38.0	41.0	34.0	41.0
22	38.47317723268624	40.0	38.0	41.0	34.0	41.0
23	38.43171910135539	40.0	37.0	41.0	34.0	41.0
24	38.38073071606874	40.0	37.0	41.0	34.0	41.0
25	38.276143832649105	40.0	37.0	41.0	34.0	41.0
26	38.21996988013946	40.0	37.0	41.0	34.0	41.0
27	38.18756998741568	40.0	37.0	41.0	34.0	41.0
28	38.124587296020465	40.0	37.0	41.0	34.0	41.0
29	38.095173808099354	40.0	37.0	41.0	34.0	41.0
30	37.846561178387965	40.0	37.0	41.0	33.0	41.0
31	37.873709487756074	40.0	37.0	41.0	33.0	41.0
32	37.813787469312814	40.0	36.0	41.0	33.0	41.0
33	37.723064798960245	40.0	36.0	41.0	33.0	41.0
34	37.634050296041096	40.0	36.0	41.0	33.0	41.0
35	37.594086604914075	40.0	36.0	41.0	33.0	41.0
36	37.49490726796361	40.0	36.0	41.0	33.0	41.0
37	37.399828358055	40.0	36.0	41.0	33.0	41.0
38	37.32389990303881	40.0	36.0	41.0	33.0	41.0
39	37.24849215026922	40.0	35.0	41.0	32.0	41.0
40	37.177008231386544	39.0	35.0	41.0	32.0	41.0
41	37.14456460297485	39.0	35.0	41.0	32.0	41.0
42	37.10341097105605	39.0	35.0	41.0	32.0	41.0
43	36.96042993006416	39.0	35.0	41.0	32.0	41.0
44	36.79634848266045	39.0	35.0	41.0	31.0	41.0
45	36.77541724258866	39.0	35.0	41.0	31.0	41.0
46	36.73938646256679	39.0	35.0	41.0	31.0	41.0
47	36.692594227714395	39.0	35.0	41.0	31.0	41.0
48	36.63249974212448	39.0	35.0	41.0	31.0	41.0
49	36.57214284240711	39.0	35.0	41.0	31.0	41.0
50	36.44938089245559	39.0	35.0	40.0	31.0	41.0
51	35.485901017061046	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	10.0
10	11.0
11	18.0
12	8.0
13	5.0
14	6.0
15	18.0
16	37.0
17	73.0
18	164.0
19	288.0
20	553.0
21	954.0
22	1465.0
23	2307.0
24	3639.0
25	5440.0
26	7204.0
27	8634.0
28	10250.0
29	12272.0
30	15550.0
31	21449.0
32	29312.0
33	46560.0
34	70600.0
35	94485.0
36	114624.0
37	194498.0
38	275097.0
39	296106.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.893136385204134	4.3037567305510285	55.54465372475399	16.258453159490852
2	20.32372661069049	4.1114022239184695	53.71542920801271	21.849441957378335
3	20.829987828275534	4.354094031729003	52.11041198192808	22.70550615806738
4	19.07090545251996	4.6329296721886415	51.22893157015246	25.06723330513894
5	17.74917995585171	8.045716171889506	50.37294988962928	23.832153982629507
6	16.09143234377901	30.77936170651703	40.39419883233965	12.735007117364304
7	85.47999917479834	2.1344666102778866	8.440492645390217	3.945041569533555
8	87.1534256183855	2.193798609535205	6.565716997091164	4.0870587749881375
9	82.06432446929219	4.428362181007984	8.746890021248943	4.760423328450891
10	40.096961194892	23.77917603614383	18.921543952303345	17.20231881666082
11	28.532667670662015	25.67334392342129	24.30631485569286	21.487673550223835
12	24.477956800693168	22.43252944938419	31.30996637303241	21.779547376890225
13	23.56322076207373	23.247746167969797	33.225011862273846	19.964021207682627
14	20.93594372124688	25.261980896581605	32.921255131722816	20.880820250448703
15	19.743444804324056	25.068141026963463	34.27615373506901	20.91226043364347
16	22.214181090503992	24.050749902007304	31.45858519175623	22.27648381573247
17	22.54104346749737	24.196645555257565	29.21469684154065	24.04761413570441
18	23.066449363563223	24.150846863202194	30.998040146060696	21.78466362717389
19	23.589792255482433	25.717079611330018	28.93239535411466	21.760732779072885
20	24.65454995564541	26.133228807789905	29.33121531574279	19.881005920821902
21	25.231448435211355	25.933282445897714	29.05815608689373	19.777113031997196
22	24.000082520165865	23.861036040682443	28.88205805293669	23.256823386215007
23	22.195283972520784	25.25183091618014	28.265137292925957	24.287747818373116
24	21.987828275534834	25.15511728178574	29.390464794834237	23.466589647845193
25	22.275988694737276	26.32946176221814	27.546964289398225	23.84758525364636
26	21.771873001464733	26.7356260186083	28.304746972541416	23.187754007385557
27	21.26371382006478	26.112268685660055	30.0487281579436	22.575289336331565
28	19.51965011449673	25.591401398716812	30.88028386937058	24.00866461741588
29	21.039093928578797	24.85556907969385	29.98642543271512	24.118911559012236
30	21.709322715738658	26.038743217873865	29.68398902481794	22.567945041569534
31	22.221690425597757	27.11563138241908	27.956429352422994	22.706248839560168
32	22.894559858065314	27.57213293998721	26.82961648752914	22.703690714418336
33	22.823097394425762	26.75897922554824	26.777298702370388	23.6406246776556
34	21.667815072308294	26.514967095083865	27.740804159016356	24.076413673591485
35	22.84867864584408	26.274008210756506	27.062488395601676	23.814824747797743
36	24.096878674726135	27.008437686959752	27.267881088440987	21.626802549873126
37	21.677304891382832	27.1361789037196	28.314731912611148	22.871784292286428
38	21.075815402388958	29.021929734078768	26.281435025684402	23.620819837847872
39	21.81420584655375	28.401708167433416	26.94283415509665	22.84125183091618
40	23.14558620262827	26.84158191157964	27.04119819280837	22.971633692983723
41	22.846368081199845	25.777979493738783	26.68644399975244	24.689208425308934
42	24.021290202793306	24.405834175726692	27.391001175912365	24.181874445567637
43	23.19988447176779	24.25185154622161	28.740040847482103	23.8082231345285
44	21.795473768902276	25.15907824974728	27.823819445877085	25.221628535473357
45	22.336228415819114	25.396323726610692	26.732160171641944	25.53528768592825
46	22.69494357683659	25.8715573618303	28.200028882058053	23.23347017927506
47	21.141831535081383	25.74662183070988	29.31190559693025	23.799641037278484
48	21.43568584572855	25.845563509582654	29.42091473603862	23.29783590865018
49	22.961401192416396	24.77651476079467	28.881232851278032	23.380851195510903
50	21.577868091514866	25.660635817877992	27.491263177438984	25.270232913168154
51	20.943040455511316	25.194066800074268	26.39927382254038	27.463618921874033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	656.5
2	1137.0
3	3188.5
4	5240.0
5	3610.5
6	1981.0
7	2005.5
8	2030.0
9	2134.0
10	2238.0
11	2291.5
12	2345.0
13	2328.0
14	2311.0
15	2265.0
16	2219.0
17	2246.0
18	2273.0
19	2436.5
20	2600.0
21	3136.5
22	3673.0
23	4301.0
24	4929.0
25	5997.5
26	8175.5
27	9285.0
28	11148.5
29	13012.0
30	15988.5
31	18965.0
32	22306.5
33	25648.0
34	29053.0
35	32458.0
36	35904.5
37	39351.0
38	43877.0
39	48403.0
40	54670.0
41	60937.0
42	69219.5
43	77502.0
44	83100.0
45	88698.0
46	100066.0
47	111434.0
48	122754.5
49	134075.0
50	127469.0
51	120863.0
52	106198.0
53	91533.0
54	80920.5
55	70308.0
56	63317.5
57	56327.0
58	51460.5
59	46594.0
60	41588.5
61	36583.0
62	32674.5
63	28766.0
64	25055.0
65	21344.0
66	17904.0
67	14464.0
68	12327.0
69	10190.0
70	8261.5
71	6333.0
72	5110.0
73	3887.0
74	3104.5
75	1779.0
76	1236.0
77	935.5
78	635.0
79	431.5
80	228.0
81	162.0
82	96.0
83	78.5
84	61.0
85	57.5
86	54.0
87	31.0
88	8.0
89	6.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1211825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24400403980711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59718574298498	25.949686893075675
2	11.283412759568797	8.179121152812453
3	4.011088109759205	4.3613368096240475
4	2.1554708208602067	3.1249157255577478
5	1.4468728716166863	2.622023310198125
6	1.091095498960516	2.372740180328418
7	0.9161351977724037	2.3243085466350744
8	0.8083184934370821	2.343735899326752
9	0.6619666673737413	2.159309030986038
>10	5.900633761368647	40.76487041637066
>50	0.09242383867665563	2.2540446594353334
>100	0.03207041672635142	1.9396779894430127
>500	0.002138027718055722	0.5723978617165182
>1k	0.001187793176697623	1.0318315244901854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	2883	0.23790563819033275	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	2863	0.23625523487302208	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	2146	0.17708827594743465	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGC	2047	0.16891877952674683	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2045	0.1687537391950158	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25432715119757393	0.0
2	0.0	0.0	0.0	0.8930332349968023	0.0
3	0.0	0.0	0.0	1.2615682957522745	0.0
4	0.0	0.0	0.0	1.6848967466424607	0.0
5	0.0	0.0	0.0	2.405380314814433	0.0
6	8.252016586553339E-5	0.0	0.0	2.9595032286014895	0.0
7	8.252016586553339E-5	0.0	0.0	3.591855259629072	0.0
8	8.252016586553339E-5	0.0	0.0	4.601406968828007	0.0
9	8.252016586553339E-5	0.0	0.0	5.078373527530791	0.0
10	8.252016586553339E-5	0.0	0.0	6.029418439131063	0.0
11	8.252016586553339E-5	0.0	0.0	7.841726321869907	0.0
12	8.252016586553339E-5	0.0	0.0	9.394013161966456	0.0
13	8.252016586553339E-5	0.0	0.0	9.930971881253482	0.0
14	8.252016586553339E-5	0.0	0.0	10.113011367152849	0.0
15	8.252016586553339E-5	0.0	0.0	10.372784849297547	0.0
16	8.252016586553339E-5	0.0	0.0	11.030140490582387	0.0
17	8.252016586553339E-5	0.0	0.0	11.976729313225919	0.0
18	8.252016586553339E-5	0.0	0.0	12.98768386524457	0.0
19	8.252016586553339E-5	0.0	0.0	13.71171580054876	0.0
20	8.252016586553339E-5	0.0	0.0	14.452251769026056	0.0
21	8.252016586553339E-5	0.0	0.0	15.545478926412642	0.0
22	8.252016586553339E-5	0.0	0.0	16.684999896849792	0.0
23	8.252016586553339E-5	0.0	0.0	17.831370041053784	0.0
24	8.252016586553339E-5	0.0	0.0	18.72357807439193	0.0
25	8.252016586553339E-5	0.0	0.0	19.489942854785138	0.0
26	8.252016586553339E-5	0.0	0.0	20.186082974026778	0.0
27	8.252016586553339E-5	0.0	0.0	20.8592824871578	0.0
28	8.252016586553339E-5	0.0	0.0	21.585212386276897	0.0
29	8.252016586553339E-5	0.0	0.0	22.340684504775854	0.0
30	8.252016586553339E-5	0.0	0.0	23.17158005487591	0.0
31	8.252016586553339E-5	0.0	0.0	23.923833886906113	0.0
32	8.252016586553339E-5	0.0	0.0	24.63936624512615	0.0
33	8.252016586553339E-5	0.0	0.0	25.362738019103418	0.0
34	8.252016586553339E-5	0.0	0.0	26.072824046376333	0.0
35	8.252016586553339E-5	0.0	0.0	26.824417717079612	0.0
36	8.252016586553339E-5	0.0	0.0	27.541517958451095	0.0
37	8.252016586553339E-5	0.0	0.0	28.2719864666928	0.0
38	8.252016586553339E-5	0.0	0.0	29.0168134837951	0.0
39	8.252016586553339E-5	0.0	0.0	29.84506838858746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAC	20	7.0334383E-4	45.0	19
TATTCGC	20	7.0334383E-4	45.0	32
CGGTCTA	25	3.890931E-5	45.0	31
GCCGATT	205	0.0	43.902443	9
CGTTAGG	330	0.0	41.590908	2
TAGGGCG	605	0.0	41.280994	5
TCGTTAG	180	0.0	41.250004	1
TTAGGGT	1280	0.0	40.95703	4
CGAGGGT	165	0.0	40.90909	4
AGGGCGA	785	0.0	40.700634	6
GTTAGGG	1765	0.0	40.66572	3
TTTAGGG	4815	0.0	40.46729	3
TTAGGGA	2430	0.0	40.27778	4
CGTTTAT	520	0.0	40.240383	1
TGTAGGG	1635	0.0	40.183483	3
CTTAGGG	2200	0.0	39.68182	3
CTCGTAG	80	0.0	39.375	1
ATTACGA	40	3.4584264E-7	39.375	29
TTGAGGG	2035	0.0	39.361176	3
CGTTTTT	1425	0.0	39.157894	1
>>END_MODULE