SRR2934294.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543257
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	6544	1.2045864112197358	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	5973	1.0994796201429524	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	5359	0.9864576066208075	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	5191	0.9555330166017189	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	3963	0.7294889895574286	No Hit
TCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	3836	0.7061114721025225	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	3703	0.6816295050040773	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	1810	0.33317564246756143	No Hit
CTGTCACTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	1705	0.31384777370563105	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	1512	0.2783213101717971	No Hit
CCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1449	0.2667245889146389	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTC	1329	0.2446355960438614	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	1315	0.242058546875604	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.21536768049008112	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCTCGGT	1162	0.21389508096536264	No Hit
GAATCAGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	1113	0.2048754088764618	No Hit
TCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1099	0.2022983597082044	No Hit
GCTGTATCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	1091	0.2008257601834859	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	1054	0.1940149873816628	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTA	1047	0.19272646279753414	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	951	0.17505526850091208	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	871	0.16032927325372706	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	855	0.15738407420429007	No Hit
TTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	736	0.1354791562741023	No Hit
TGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	705	0.1297728331158181	No Hit
GCCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	700	0.12885245841286905	No Hit
CGTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	601	0.11062903929447757	No Hit
CGCTGACTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	572	0.10529086601737299	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTT	45	3.8380676E-10	45.000004	6
GCGACTT	20	7.029999E-4	45.0	9
CGCGGGT	40	6.8030204E-9	45.0	4
CGACCCT	20	7.029999E-4	45.0	12
CGACAAT	25	3.8880808E-5	45.0	20
GCGTTAG	40	6.8030204E-9	45.0	1
GTCCGCT	20	7.029999E-4	45.0	40
GGTGCGT	25	3.8880808E-5	45.0	8
CGAGATT	35	1.2103374E-7	45.0	27
TATCGCG	20	7.029999E-4	45.0	1
CGATAGG	25	3.8880808E-5	45.0	2
ACAAGCG	55	1.8189894E-12	45.0	14
CTAGCGG	35	1.2103374E-7	45.0	2
TGTTCCG	30	2.1633805E-6	44.999996	1
CGTTTAT	360	0.0	44.375004	1
CGTTTTT	830	0.0	43.64458	1
CGTTAGG	120	0.0	43.124996	2
GTATCTT	470	0.0	42.12766	4
TTAGGGA	1280	0.0	42.01172	4
CTAGGGT	135	0.0	41.666664	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content