##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934293.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225408
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10702721052906	33.0	31.0	34.0	30.0	34.0
2	32.261399468585154	34.0	31.0	34.0	30.0	34.0
3	32.29890371206977	34.0	31.0	34.0	30.0	34.0
4	35.867615520708206	37.0	35.0	37.0	35.0	37.0
5	34.99997225413903	37.0	35.0	37.0	35.0	37.0
6	25.772754054159922	35.0	0.0	37.0	0.0	37.0
7	30.936631717762573	36.0	19.0	37.0	17.0	37.0
8	34.68518322060897	37.0	32.0	37.0	32.0	37.0
9	37.26018109886666	39.0	37.0	39.0	35.0	39.0
10	37.10647474147386	39.0	37.0	39.0	34.0	39.0
11	37.14583958975297	39.0	37.0	39.0	34.0	39.0
12	37.096200612367475	39.0	37.0	39.0	34.0	39.0
13	37.170615827544786	39.0	37.0	39.0	34.0	39.0
14	38.30598217083616	40.0	38.0	41.0	34.0	41.0
15	38.392483972685014	40.0	38.0	41.0	34.0	41.0
16	38.45566864260719	40.0	38.0	41.0	34.0	41.0
17	38.451536957486816	40.0	38.0	41.0	34.0	41.0
18	38.427838727999166	40.0	38.0	41.0	34.0	41.0
19	38.41005118295294	40.0	37.0	41.0	34.0	41.0
20	38.32887087402726	40.0	37.0	41.0	34.0	41.0
21	38.24000414555805	40.0	37.0	41.0	34.0	41.0
22	38.25701072622343	40.0	37.0	41.0	34.0	41.0
23	38.19378198934559	40.0	37.0	41.0	34.0	41.0
24	38.12346173682561	40.0	37.0	41.0	34.0	41.0
25	38.02123456024442	40.0	37.0	41.0	34.0	41.0
26	37.96934327179193	40.0	37.0	41.0	34.0	41.0
27	37.91655269102209	40.0	37.0	41.0	34.0	41.0
28	37.86322922651068	40.0	37.0	41.0	33.0	41.0
29	37.87521380634042	40.0	37.0	41.0	33.0	41.0
30	37.5981191570481	40.0	36.0	41.0	33.0	41.0
31	37.59029237609025	40.0	36.0	41.0	33.0	41.0
32	37.5044189363869	40.0	36.0	41.0	33.0	41.0
33	37.333327348931945	39.0	36.0	41.0	32.0	41.0
34	37.26740073510211	39.0	36.0	41.0	32.0	41.0
35	37.20054626703922	40.0	36.0	41.0	32.0	41.0
36	36.97140544210581	39.0	36.0	41.0	31.0	41.0
37	36.862049211364706	39.0	35.0	41.0	31.0	41.0
38	36.85771188045125	39.0	36.0	41.0	31.0	41.0
39	36.86744904554238	39.0	36.0	41.0	31.0	41.0
40	36.88798914320781	39.0	36.0	41.0	31.0	41.0
41	36.74452508878675	39.0	35.0	41.0	31.0	41.0
42	36.54382213923852	39.0	35.0	40.0	30.0	41.0
43	36.40306575442628	39.0	35.0	40.0	30.0	41.0
44	36.32828086645427	39.0	35.0	40.0	30.0	41.0
45	36.36612377265368	39.0	35.0	40.0	30.0	41.0
46	36.21372636705489	39.0	35.0	40.0	29.0	41.0
47	36.051209882749255	39.0	35.0	40.0	29.0	41.0
48	35.97522457826291	39.0	35.0	40.0	29.0	41.0
49	35.94143419987466	39.0	35.0	40.0	29.0	41.0
50	35.83855662767013	38.0	35.0	40.0	29.0	41.0
51	34.77337262364861	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	1.0
12	4.0
13	7.0
14	5.0
15	19.0
16	36.0
17	65.0
18	127.0
19	304.0
20	560.0
21	1141.0
22	1858.0
23	3083.0
24	5176.0
25	7996.0
26	10685.0
27	11800.0
28	12707.0
29	14825.0
30	18616.0
31	24559.0
32	33659.0
33	49476.0
34	71663.0
35	98060.0
36	132926.0
37	219249.0
38	279666.0
39	227042.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.726056954092027	4.366300856531049	60.305057580822066	11.602584608554865
2	22.131159581135424	4.073173995926255	52.54576434950645	21.24990207343187
3	18.328426124197	3.654211495273411	55.04150454379276	22.975857836736825
4	16.6741199665744	4.090392750822583	55.42260210476837	23.812885177834648
5	15.664252232725753	7.152801352692329	54.33520917114952	22.84773724343239
6	13.784388546508591	30.929943333159244	42.653467253355615	12.63220086697655
7	81.1512573771348	0.8013657492035305	12.849271426333106	5.1981054473285635
8	82.52435107327518	1.0221085548649917	10.246220034470152	6.207320337389669
9	78.36940839295973	2.749370005745025	12.966456886196271	5.914764715098971
10	36.136862171619576	23.529714184989817	23.369685851569436	16.963737791821174
11	22.282700945317803	27.03197628871364	28.456399827649243	22.228922938319318
12	20.063684911474382	22.399070350446546	34.91783960933827	22.619405128740794
13	21.63801770512352	24.965317673787016	35.45814944899984	17.938515172089623
14	20.05650362981146	26.561194312424924	32.8618713114326	20.520430746331016
15	16.487080221444614	27.916171593461115	34.82554381887502	20.77120436621925
16	17.72242387841437	26.77500065284379	31.8626938946049	23.63988157413694
17	17.96307841959576	27.918619757664388	29.183994228860914	24.934307593878938
18	19.262808795111503	25.613591554812764	33.83509818770565	21.288501462370085
19	19.21351908915235	30.11103240716561	30.22470883167076	20.45073967201128
20	20.023208596646995	29.552361335979526	30.923006867916648	19.501423199456834
21	19.682668955972215	29.565744633624064	31.31259139813026	19.438995012273462
22	18.46837951115057	26.8099277954771	29.388742361727687	25.332950331644643
23	17.92758003864835	27.552292787381838	28.19730244946989	26.32282472449992
24	19.066139604115527	27.026998354833655	29.786405833812086	24.120456207238732
25	18.54582310544733	28.16980140491983	27.33856805243641	25.94580743719643
26	18.50502036872617	29.648819005588344	29.330231237269544	22.51592938841594
27	18.834298454065912	29.499970622029558	29.4379504622134	22.227780461691125
28	16.35406329973364	27.732640883689353	30.7344982242649	25.17879759231211
29	17.446760589126235	25.196832401942864	30.813165900663293	26.543241108267612
30	20.49431699482948	28.265932652634877	29.61021961665013	21.629530735885517
31	19.925853266830313	31.3317686843892	26.419445605055625	22.322932443724866
32	19.572175144931318	30.689533608398182	25.829927664908343	23.908363581762156
33	19.55038648352222	28.070895571107744	28.454604507233512	23.924113438136523
34	19.786960751031494	27.1167643756202	28.081504282655246	25.014770590693058
35	21.70215960724918	25.16541429466757	26.874722541390295	26.257703556692952
36	21.40642137149423	29.738176999007678	28.048209249490778	20.80719238000731
37	18.349806758238888	31.82809317386536	28.659515851047164	21.162584216848593
38	17.341489528385647	29.810153026583798	28.34288661409098	24.505470830939576
39	19.066955658849952	24.453080116989607	32.9436399958218	23.536324228338643
40	24.612863633989658	23.064073353527967	30.34156786964015	21.98149514284222
41	24.395221836319006	24.770443803206767	27.333263696662662	23.501070663811564
42	22.683873452760224	25.97543022405599	29.10940682613464	22.231289497049143
43	19.98256907087272	26.279410612628613	30.323696270956287	23.414324045542383
44	18.586707447641928	25.944583355094792	26.963590901968974	28.505118295294302
45	22.225332297487856	23.41489528385648	26.228896824567816	28.130875594087847
46	23.65579464145819	24.93447080482582	28.2715634303024	23.13817112341359
47	20.200945317804354	27.020551522431713	30.16791142215491	22.610591737609028
48	18.945526714367787	26.16018501592939	30.439494437770932	24.454793831931894
49	21.312085444194913	23.165345746069878	32.0559356557163	23.466633154018908
50	21.20167323862746	21.297314853501852	29.614381495795687	27.886630412074997
51	20.797318117720792	20.897774455528282	27.054825821277483	31.25008160547344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	234.5
2	395.0
3	4398.0
4	8401.0
5	5750.0
6	3099.0
7	3213.0
8	3327.0
9	3742.0
10	4157.0
11	4426.5
12	4696.0
13	4839.0
14	4982.0
15	5016.0
16	5050.0
17	5107.0
18	5164.0
19	5250.5
20	5337.0
21	5261.5
22	5186.0
23	5525.5
24	5865.0
25	6217.5
26	7469.5
27	8369.0
28	9627.0
29	10885.0
30	12193.0
31	13501.0
32	16869.0
33	20237.0
34	23881.5
35	27526.0
36	32209.5
37	36893.0
38	41427.5
39	45962.0
40	53955.0
41	61948.0
42	72786.0
43	83624.0
44	97579.0
45	111534.0
46	137419.0
47	163304.0
48	182132.5
49	200961.0
50	182417.5
51	163874.0
52	129858.0
53	95842.0
54	73856.0
55	51870.0
56	40069.0
57	28268.0
58	22100.0
59	15932.0
60	12816.0
61	9700.0
62	7739.0
63	5778.0
64	4383.5
65	2989.0
66	2315.0
67	1641.0
68	1375.5
69	1110.0
70	851.5
71	593.0
72	490.5
73	388.0
74	309.5
75	152.0
76	73.0
77	55.0
78	37.0
79	32.5
80	28.0
81	15.5
82	3.0
83	2.0
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1225408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.76537754024854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19997518809876	33.95789877960414
2	12.9681299872864	11.869827297183612
3	4.558293350128105	6.2583604832345126
4	2.2365238928805757	4.094214413418637
5	1.2759313304564295	2.9196739526885054
6	0.8765903115920005	2.40704919348792
7	0.6114665210174477	1.9588797331310068
8	0.47660006100776986	1.744942538218086
9	0.36382269583224486	1.4985434729226323
>10	2.2471925256316694	18.390364273903803
>50	0.11018064488768871	3.483445260439768
>100	0.06709321155004055	5.709338878935492
>500	0.004659249790314175	1.5123289468517884
>1k	0.0029819198658010723	2.700288879836488
>5k	5.59109974837701E-4	1.4948438961437283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	6327	0.5163178304695253	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	5870	0.4790241291063874	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	5329	0.4348755679740951	No Hit
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	4724	0.3855042565414947	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4096	0.3342560192197211	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	2390	0.1950370815271322	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	2375	0.19381299942549746	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	2063	0.1683520917114953	No Hit
TCTGTATCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1922	0.1568457199561289	No Hit
CTGTCACTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	1804	0.14721627408993576	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1792	0.14623700840862797	No Hit
TTCCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1714	0.13987178148012744	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1535	0.12526440173395312	No Hit
CCTGTATCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1488	0.12142894448216432	No Hit
TGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	1294	0.10559748263435526	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	1281	0.10453661147960516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.160547344231472E-5	0.0	0.0	0.5518162114169322	0.0
2	8.160547344231472E-5	0.0	0.0	1.9830130046482477	0.0
3	8.160547344231472E-5	0.0	0.0	2.9067053585418083	0.0
4	8.160547344231472E-5	0.0	0.0	3.9717383924374574	0.0
5	8.160547344231472E-5	0.0	0.0	5.690268057659163	0.0
6	8.160547344231472E-5	0.0	0.0	6.95368399749308	0.0
7	8.160547344231472E-5	0.0	0.0	8.369130934350029	0.0
8	8.160547344231472E-5	0.0	0.0	10.575906147177104	0.0
9	8.160547344231472E-5	0.0	0.0	11.474300804303546	0.0
10	8.160547344231472E-5	0.0	0.0	13.50056470987622	0.0
11	8.160547344231472E-5	0.0	0.0	17.6621174335405	0.0
12	8.160547344231472E-5	0.0	0.0	20.7574130412075	0.0
13	8.160547344231472E-5	0.0	0.0	21.849049459445343	0.0
14	8.160547344231472E-5	0.0	0.0	22.181918185616546	0.0
15	8.160547344231472E-5	0.0	0.0	22.63393090301353	0.0
16	8.160547344231472E-5	0.0	0.0	23.887309369614037	0.0
17	8.160547344231472E-5	0.0	0.0	25.864609991121323	0.0
18	8.160547344231472E-5	0.0	0.0	27.942122134015772	0.0
19	8.160547344231472E-5	0.0	0.0	29.380908236277225	0.0
20	8.160547344231472E-5	0.0	0.0	30.702916906042724	0.0
21	8.160547344231472E-5	0.0	0.0	32.883904789262026	0.0
22	8.160547344231472E-5	0.0	0.0	35.15596438084295	0.0
23	8.160547344231472E-5	0.0	0.0	37.63065036298114	0.0
24	8.160547344231472E-5	0.0	0.0	39.28707826291325	0.0
25	8.160547344231472E-5	0.0	0.0	40.710522536167545	0.0
26	8.160547344231472E-5	0.0	0.0	41.942357157779284	0.0
27	8.160547344231472E-5	0.0	0.0	43.130124823732174	0.0
28	8.160547344231472E-5	0.0	0.0	44.419654515067634	0.0
29	8.160547344231472E-5	0.0	0.0	45.782873948921505	0.0
30	8.160547344231472E-5	0.0	0.0	47.11598096307515	0.0
31	8.160547344231472E-5	0.0	0.0	48.41864913563482	0.0
32	8.160547344231472E-5	0.0	0.0	49.56039131456625	0.0
33	8.160547344231472E-5	0.0	0.0	50.688260563012484	0.0
34	8.160547344231472E-5	0.0	0.0	51.7530487804878	0.0
35	8.160547344231472E-5	0.0	0.0	52.85921097299838	0.0
36	8.160547344231472E-5	0.0	0.0	53.92563129994255	0.0
37	8.160547344231472E-5	0.0	0.0	54.98617603279887	0.0
38	8.160547344231472E-5	0.0	0.0	55.99938959105865	0.0
39	8.160547344231472E-5	0.0	0.0	57.036431947563585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGCG	45	3.8562575E-10	45.000004	1
AATCCGG	20	7.0334703E-4	45.0	2
TCGTTGA	20	7.0334703E-4	45.0	24
TCGTTAT	20	7.0334703E-4	45.0	1
CAACGCT	20	7.0334703E-4	45.0	37
GCACGAT	20	7.0334703E-4	45.0	9
GTCTACG	40	6.8157533E-9	45.0	1
CGACATC	20	7.0334703E-4	45.0	29
AGGCGGC	20	7.0334703E-4	45.0	6
CCGTACG	20	7.0334703E-4	45.0	31
TACGAAT	80	0.0	45.0	12
TGCGAAG	20	7.0334703E-4	45.0	1
CTCGCAG	20	7.0334703E-4	45.0	1
GCGGCTA	40	6.8157533E-9	45.0	24
CTCGAAG	25	3.890957E-5	45.0	17
GCTACGA	80	0.0	45.0	10
CGCCGTA	20	7.0334703E-4	45.0	29
CGGTACC	20	7.0334703E-4	45.0	15
GCTCGGA	20	7.0334703E-4	45.0	19
AGGGCGC	40	6.8157533E-9	45.0	6
>>END_MODULE