FastQCFastQC Report
Sat 14 Jan 2017
SRR2934292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392512
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122670.8809259812482765No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC91050.6538543294420442No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG79260.5691871955142936No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC69640.5001034102399118No Hit
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT55580.3991348009927383TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46660.33507790238073354No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC41280.2964426877470355TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC41270.2963708750804302TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC37600.2700156264362533TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG28550.20502516315837852TruSeq Adapter, Index 21 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC23680.1700523945215553No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT21280.15281735453626252No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC21220.1523864785366302No Hit
CTGTCACTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT17460.12538491589300485TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCC17380.12481041456016179No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG15900.11418213990256458TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT15630.11224319790421915No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGGTTT15330.11008881790605754No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC14700.1055646199099182No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTA14520.10427199191102123No Hit
TCTGTATCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC14130.10147129791341115TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC14080.10111223458038424TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT13960.10025048258111958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA253.8912312E-545.0000048
AGATCGC253.8912312E-545.00000412
ACTTGCG453.8562575E-1045.0000041
GCGTTAG900.045.0000041
CCGCTAG351.2121745E-745.0000041
ATCGCAC253.8912312E-545.00000414
CGAACGA207.0338015E-445.022
TTGTACG406.8175723E-945.01
TATGCGA207.0338015E-445.026
CGAGACG207.0338015E-445.033
AGCTACG551.8189894E-1245.09
CCGTTCG207.0338015E-445.01
GCCGTCG207.0338015E-445.01
ACGAACG207.0338015E-445.021
CGTTTAT28200.044.8404271
CGTTTTT65450.044.24371
TAGGGCG5150.042.8155335
CCGTAGG800.042.18752
AGGCGAT750.042.06
ATCCGGC750.042.06
Produced by FastQC (version 0.11.5)