##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1392512
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19921336405	33.0	31.0	34.0	30.0	34.0
2	32.37548832613292	34.0	31.0	34.0	30.0	34.0
3	32.35793731041456	34.0	31.0	34.0	30.0	34.0
4	35.94750996759812	37.0	35.0	37.0	35.0	37.0
5	35.06525904265098	37.0	35.0	37.0	35.0	37.0
6	25.81454378849159	36.0	0.0	37.0	0.0	37.0
7	30.954462869978858	37.0	19.0	37.0	17.0	37.0
8	34.64100201649968	37.0	32.0	37.0	32.0	37.0
9	37.24160868990716	39.0	37.0	39.0	34.0	39.0
10	37.28337565493152	39.0	37.0	39.0	34.0	39.0
11	37.34438985085946	39.0	37.0	39.0	34.0	39.0
12	37.25856366049269	39.0	37.0	39.0	34.0	39.0
13	37.338799234764224	39.0	37.0	39.0	34.0	39.0
14	38.57929123770567	40.0	38.0	41.0	34.0	41.0
15	38.64559156402243	40.0	38.0	41.0	35.0	41.0
16	38.70751275392959	40.0	38.0	41.0	35.0	41.0
17	38.668467488969576	40.0	38.0	41.0	35.0	41.0
18	38.60073593620737	40.0	38.0	41.0	35.0	41.0
19	38.542309868783896	40.0	37.0	41.0	35.0	41.0
20	38.41164384938873	40.0	37.0	41.0	34.0	41.0
21	38.31963027966725	40.0	37.0	41.0	34.0	41.0
22	38.31344002780587	40.0	37.0	41.0	34.0	41.0
23	38.22517579740785	40.0	37.0	41.0	34.0	41.0
24	38.16801506916996	40.0	37.0	41.0	34.0	41.0
25	38.04004992416582	40.0	36.0	41.0	34.0	41.0
26	37.99983195836014	40.0	36.0	41.0	34.0	41.0
27	37.96515721228973	40.0	36.0	41.0	34.0	41.0
28	37.91163235936207	40.0	36.0	41.0	34.0	41.0
29	37.86810813838588	40.0	36.0	41.0	34.0	41.0
30	37.62380719160768	40.0	36.0	41.0	33.0	41.0
31	37.56822346952845	40.0	36.0	41.0	33.0	41.0
32	37.269828913503076	40.0	36.0	41.0	33.0	41.0
33	37.06534449972424	40.0	36.0	41.0	32.0	41.0
34	36.832239147669824	40.0	36.0	41.0	31.0	41.0
35	36.62372101640776	39.0	35.0	41.0	30.0	41.0
36	36.5481252585256	39.0	35.0	41.0	30.0	41.0
37	36.50504627608236	39.0	35.0	41.0	30.0	41.0
38	36.39006988808714	39.0	35.0	41.0	30.0	41.0
39	36.37659280494531	39.0	35.0	41.0	30.0	41.0
40	36.28971886777277	39.0	35.0	41.0	30.0	41.0
41	36.11852249747219	39.0	35.0	41.0	29.0	41.0
42	36.01116471527714	39.0	35.0	41.0	29.0	41.0
43	36.06271974675981	39.0	35.0	41.0	29.0	41.0
44	36.0397246127861	39.0	35.0	41.0	29.0	41.0
45	36.04359028862947	39.0	35.0	41.0	29.0	41.0
46	35.945032430600236	39.0	35.0	41.0	28.0	41.0
47	35.808596263443334	39.0	35.0	40.0	28.0	41.0
48	35.76842713025094	39.0	35.0	40.0	27.0	41.0
49	35.75484520061587	39.0	35.0	40.0	27.0	41.0
50	35.67790367336152	39.0	35.0	40.0	27.0	41.0
51	34.72085267487821	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	12.0
11	4.0
12	5.0
13	5.0
14	3.0
15	17.0
16	30.0
17	76.0
18	228.0
19	476.0
20	966.0
21	1682.0
22	2854.0
23	5054.0
24	8519.0
25	14350.0
26	18581.0
27	18554.0
28	17569.0
29	17895.0
30	20210.0
31	25768.0
32	34492.0
33	50576.0
34	74884.0
35	106686.0
36	136865.0
37	224595.0
38	308274.0
39	303119.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.290419041272177	4.047864578545822	55.40842736005147	16.253289020130527
2	23.98169638753562	6.735956314918651	49.34248322456108	19.93986407298465
3	17.51029793639121	6.691073398290285	55.553129883261334	20.245498782057176
4	16.031818756319517	3.716161871495542	57.82872966265282	22.423289709532128
5	17.589004618990717	6.930784079419064	53.743235246805774	21.736976054784446
6	13.611947329717806	32.474477778288446	42.01356972148175	11.900005170511996
7	77.22561816343413	1.1642987636731317	16.915473618898798	4.694609453993933
8	78.46050877838037	3.0516792673959006	13.325271164629102	5.162540789594631
9	73.94514374023348	3.4615859683794463	15.06586657781046	7.527403713576615
10	34.91639569353801	26.38009582682232	23.89121242761283	14.812296052026841
11	22.211729593712658	24.303632571927565	32.40288054968288	21.0817572846769
12	19.25204235223826	21.027466908723227	37.697125769831786	22.023364969206728
13	19.97318515028955	22.580200386064895	39.47384295431565	17.972771509329903
14	18.628492968103686	26.25241290559794	35.696712128872136	19.422381997426232
15	16.067078775622758	25.115402955234856	39.4664462496553	19.351072019487084
16	17.02936850813494	25.702327879400684	35.15237211600331	22.115931496461073
17	17.788572019487084	26.153526863682323	33.70211531390753	22.355785802923062
18	18.10964645187977	24.754041616876552	35.67610189355639	21.46021003768729
19	18.4260530609431	27.719545684345988	33.19949846033643	20.654902794374483
20	19.35021026748782	27.349854076661455	34.52314953120691	18.77678612464381
21	19.29742795753286	29.09052130250942	33.91439355639305	17.697657183564665
22	18.79818629929221	24.20639822134387	33.18362786101664	23.811787618347275
23	17.93061747403254	27.230501424763304	31.9059368967736	22.932944204430555
24	19.155741566320433	25.523729777553083	32.67612774611637	22.644400910010113
25	17.355972515856237	29.47909964151117	30.19263029690229	22.972297545730306
26	17.464912331096606	28.37002481845758	31.789887627539297	22.375175222906517
27	19.143892476330546	27.610462243772403	31.267378665318503	21.978266614578544
28	16.33572996139351	26.09959555106168	33.63899197996139	23.925682507583417
29	18.039772727272727	25.54886421086497	32.50894785825903	23.902415203603272
30	21.81259479271992	26.744688735177867	30.928063815608052	20.51465265649416
31	21.429330591046973	27.494987475870946	28.226758548579834	22.84892338450225
32	19.538072203327513	30.570580361246435	28.817130480742716	21.074216954683333
33	21.9376206452799	26.116902403713578	28.84370116738671	23.101775783619818
34	20.746392131629747	26.63014753194227	29.236013765051933	23.387446571376046
35	19.71444411251034	27.321559886018935	29.747319951282286	23.216676050188436
36	21.97216253791709	27.21125563011306	29.57841655942642	21.238165272543434
37	20.649660469712288	27.07789950822686	31.080881170144316	21.191558851916536
38	19.23739256825076	27.575345849802375	30.133528472286052	23.053733109660815
39	21.857549522014892	26.779589691148082	30.704295546465666	20.658565240371356
40	23.909165594264177	24.549160079051383	31.17517120139719	20.36650312528725
41	21.033930048717714	25.167179887857337	31.388526633881792	22.410363429543157
42	19.5712496552992	24.8013661641695	33.64617324662193	21.981210933909367
43	19.979791915617245	24.543702316389375	32.779753424027945	22.696752343965436
44	19.95228766430738	25.387788399669088	29.021724767901464	25.63819916812207
45	21.129799958635903	24.59935713300855	27.701664330361247	26.5691785779943
46	23.98851859086313	25.315257606397644	28.57095665961945	22.12526714311977
47	19.416134295431565	25.099101479915433	32.716055588748965	22.768708635904037
48	19.458503768728743	25.16344562919386	31.522744507767257	23.855306094310137
49	20.905313562827466	22.80195790054233	33.304632204246715	22.988096332383492
50	21.481969275668718	21.98911032723596	31.221274933357847	25.307645463737476
51	20.069844999540397	21.38609936575053	29.511343528816987	29.032712105892085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	401.5
2	637.0
3	12689.5
4	24742.0
5	16303.0
6	7864.0
7	7892.5
8	7921.0
9	8516.5
10	9112.0
11	9595.0
12	10078.0
13	9989.0
14	9900.0
15	9587.5
16	9275.0
17	8792.5
18	8310.0
19	8164.0
20	8018.0
21	7717.0
22	7416.0
23	7381.5
24	7347.0
25	8444.0
26	10517.5
27	11494.0
28	13398.5
29	15303.0
30	17228.0
31	19153.0
32	23242.5
33	27332.0
34	31211.5
35	35091.0
36	39076.5
37	43062.0
38	47855.0
39	52648.0
40	60277.0
41	67906.0
42	78773.0
43	89640.0
44	103761.0
45	117882.0
46	154328.5
47	190775.0
48	189912.0
49	189049.0
50	172425.5
51	155802.0
52	126663.5
53	97525.0
54	79269.0
55	61013.0
56	48934.5
57	36856.0
58	30824.0
59	24792.0
60	20229.0
61	15666.0
62	12744.5
63	9823.0
64	7736.5
65	5650.0
66	4272.0
67	2894.0
68	2154.5
69	1415.0
70	986.5
71	558.0
72	487.0
73	416.0
74	300.5
75	139.0
76	93.0
77	95.0
78	97.0
79	65.0
80	33.0
81	22.5
82	12.0
83	9.0
84	6.0
85	9.5
86	13.0
87	7.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1392512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.62227574785929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82101902392436	31.8904210457353
2	11.62451790570935	9.909268152261433
3	4.238516920490742	5.4196571084137135
4	2.1480819519573426	3.662245651413027
5	1.3273740216269927	2.828785078516531
6	0.9380223390505615	2.3988388075598994
7	0.6785105688441853	2.024376519417961
8	0.5410546571741728	1.8448784634192854
9	0.44644300435049733	1.7125575153416677
>10	3.0635879587536454	23.031717509119133
>50	0.1091370522348368	3.193894806738294
>100	0.055358051982722006	4.251731270002191
>500	0.0032777780480549067	0.8997865717851626
>1k	0.004188271950292381	3.6859970741524335
>5k	7.283951217899793E-4	2.293745584056492
>10k+	1.8209878044749483E-4	0.9520988420675053
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12267	0.8809259812482765	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGC	9105	0.6538543294420442	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCG	7926	0.5691871955142936	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	6964	0.5001034102399118	No Hit
CTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	5558	0.3991348009927383	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4666	0.33507790238073354	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	4128	0.2964426877470355	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	4127	0.2963708750804302	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	3760	0.2700156264362533	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	2855	0.20502516315837852	TruSeq Adapter, Index 21 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	2368	0.1700523945215553	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	2128	0.15281735453626252	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTC	2122	0.1523864785366302	No Hit
CTGTCACTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGCT	1746	0.12538491589300485	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCC	1738	0.12481041456016179	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTG	1590	0.11418213990256458	TruSeq Adapter, Index 21 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCT	1563	0.11224319790421915	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAGGTTT	1533	0.11008881790605754	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTC	1470	0.1055646199099182	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTA	1452	0.10427199191102123	No Hit
TCTGTATCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	1413	0.10147129791341115	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCTTCTGC	1408	0.10111223458038424	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAGGTTTCGTATGCCGTCT	1396	0.10025048258111958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44365865428807794	0.0
2	0.0	0.0	0.0	1.4349607041088335	0.0
3	0.0	0.0	0.0	2.1101433955326776	0.0
4	0.0	0.0	0.0	2.990279437448295	0.0
5	0.0	0.0	0.0	4.740066871955143	0.0
6	0.0	0.0	0.0	5.8976870576339735	0.0
7	0.0	0.0	0.0	7.281660768452983	0.0
8	0.0	0.0	0.0	9.209112740141558	0.0
9	0.0	0.0	0.0	10.0642579740785	0.0
10	0.0	0.0	0.0	12.090883238349113	0.0
11	0.0	0.0	0.0	14.858830659987131	0.0
12	0.0	0.0	0.0	17.95769084934277	0.0
13	0.0	0.0	0.0	18.86425395256917	0.0
14	0.0	0.0	0.0	19.210247380273923	0.0
15	0.0	0.0	0.0	19.684857293868923	0.0
16	0.0	0.0	0.0	20.53928440113981	0.0
17	0.0	0.0	0.0	21.852307197352697	0.0
18	0.0	0.0	0.0	23.20432427153231	0.0
19	0.0	0.0	0.0	24.36072364187885	0.0
20	0.0	0.0	0.0	25.345562551705118	0.0
21	0.0	0.0	0.0	26.744329671844838	0.0
22	0.0	0.0	0.0	28.276237475870943	0.0
23	0.0	0.0	0.0	29.77906114992187	0.0
24	0.0	0.0	0.0	30.86644854766063	0.0
25	0.0	0.0	0.0	31.80848710819009	0.0
26	0.0	0.0	0.0	32.630024014155715	0.0
27	0.0	0.0	0.0	33.45536699145142	0.0
28	0.0	0.0	0.0	34.33923729203052	0.0
29	0.0	0.0	0.0	35.246949397922606	0.0
30	0.0	0.0	0.0	36.193799407114625	0.0
31	0.0	0.0	0.0	37.10050613567424	0.0
32	0.0	0.0	0.0	37.96771589760088	0.0
33	0.0	0.0	0.0	38.80282539755492	0.0
34	0.0	0.0	0.0	39.592118416214724	0.0
35	0.0	0.0	0.0	40.4464018981524	0.0
36	0.0	0.0	0.0	41.25185276679842	0.0
37	0.0	0.0	0.0	42.06628021877011	0.0
38	0.0	0.0	0.0	42.88752987406931	0.0
39	0.0	0.0	0.0	43.70518889603824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	25	3.8912312E-5	45.000004	8
AGATCGC	25	3.8912312E-5	45.000004	12
ACTTGCG	45	3.8562575E-10	45.000004	1
GCGTTAG	90	0.0	45.000004	1
CCGCTAG	35	1.2121745E-7	45.000004	1
ATCGCAC	25	3.8912312E-5	45.000004	14
CGAACGA	20	7.0338015E-4	45.0	22
TTGTACG	40	6.8175723E-9	45.0	1
TATGCGA	20	7.0338015E-4	45.0	26
CGAGACG	20	7.0338015E-4	45.0	33
AGCTACG	55	1.8189894E-12	45.0	9
CCGTTCG	20	7.0338015E-4	45.0	1
GCCGTCG	20	7.0338015E-4	45.0	1
ACGAACG	20	7.0338015E-4	45.0	21
CGTTTAT	2820	0.0	44.840427	1
CGTTTTT	6545	0.0	44.2437	1
TAGGGCG	515	0.0	42.815533	5
CCGTAGG	80	0.0	42.1875	2
AGGCGAT	75	0.0	42.0	6
ATCCGGC	75	0.0	42.0	6
>>END_MODULE