FastQCFastQC Report
Sat 14 Jan 2017
SRR2934290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533260
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT72980.47597928596585054TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC64310.41943310332233275TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC46530.3034710355712665TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC44510.29029649244094285TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40140.2617951293322724No Hit
CTGTCACTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT20880.1361804260203749TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC19770.1289409493497515No Hit
CGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG17420.11361412937140472TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC17380.1133532473292201TruSeq Adapter, Index 21 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG16760.1093095756753584TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG16280.10617899116914288No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA207.0340256E-445.037
CGCTATC253.891417E-545.02
CGATCGT207.0340256E-445.010
TTAGGGA28750.041.7913064
TCAAGCG1400.041.78571317
TTTAGGG54200.041.7619933
TAGGGCG6050.041.280995
TAGGGAC12000.041.06255
CGAATAT1600.040.7812514
GCGTTAG1050.040.7142871
CTTAGGG23350.040.663813
TAGGGAG16250.040.5692335
AGGGCGA8050.040.527956
CGTTTTT21350.040.3631
TCGTTAG1900.040.2631571
TGTAGGG17950.040.236773
CGTTTAT7500.040.1999971
TCTAGGG24600.039.9695173
TTAGGGG50400.039.9107134
TAGGGGA27200.039.78865
Produced by FastQC (version 0.11.5)