FastQCFastQC Report
Sat 14 Jan 2017
SRR2934289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827856
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT63890.7717525753271101No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC60980.736601534566398No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC46680.5638661796254422No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC37770.4562387661622311No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.35875804487736995No Hit
CTGTCACTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT20020.24182949691733827No Hit
CCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC17960.21694594228948028No Hit
TCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG16750.2023298737944763No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG15620.1886801569355057No Hit
TCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC14680.1773255252121142No Hit
GCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC12800.15461626176533116No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT11990.1448319514504938No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC11520.13915463558879806No Hit
TGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG10580.12780000386540655No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.12345142150325661No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC10030.12115633636767748No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC9310.11245917164337758No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG9290.11221758373436927No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG9040.10919773487176514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCG207.0321414E-445.00000420
GATCGCG207.0321414E-445.00000410
CGGCCAC700.045.0000046
CGTCTAC207.0321414E-445.00000420
TGACGCC207.0321414E-445.00000432
CTGCGCG750.045.0000041
CGTAGTT207.0321414E-445.00000424
TATAGCG207.0321414E-445.0000041
CCACGGT207.0321414E-445.00000437
TCGACCC207.0321414E-445.00000426
GAGATCG207.0321414E-445.0000049
ATAGCGG253.889857E-545.02
TGTGCGA253.889857E-545.017
CGTTCAT253.889857E-545.017
GCGATAC253.889857E-545.09
CCGAATC502.1827873E-1145.026
CTCGTAG302.164763E-644.9999961
CATGACG302.164763E-644.99999619
ACTTACG302.164763E-644.99999627
AACGCAT302.164763E-644.99999638
Produced by FastQC (version 0.11.5)