##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827856
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14452755068514	33.0	31.0	34.0	30.0	34.0
2	32.30854882974817	34.0	31.0	34.0	30.0	34.0
3	32.35722637753426	34.0	31.0	34.0	30.0	34.0
4	35.91620160994182	37.0	35.0	37.0	35.0	37.0
5	35.1132455402872	37.0	35.0	37.0	35.0	37.0
6	26.433070485688333	36.0	0.0	37.0	0.0	37.0
7	31.25599983572022	37.0	19.0	37.0	17.0	37.0
8	34.730002560831835	37.0	32.0	37.0	32.0	37.0
9	37.225269853694364	39.0	37.0	39.0	34.0	39.0
10	37.20995680408187	39.0	37.0	39.0	34.0	39.0
11	37.25429301714308	39.0	37.0	39.0	34.0	39.0
12	37.121850901606074	39.0	37.0	39.0	34.0	39.0
13	37.13943125374461	39.0	37.0	39.0	33.0	39.0
14	38.29353172532421	40.0	38.0	41.0	34.0	41.0
15	38.37912632148586	40.0	38.0	41.0	34.0	41.0
16	38.437978344059836	40.0	38.0	41.0	34.0	41.0
17	38.44130138574825	40.0	38.0	41.0	34.0	41.0
18	38.42975710751628	40.0	38.0	41.0	34.0	41.0
19	38.43443908119286	40.0	37.0	41.0	34.0	41.0
20	38.35491558918459	40.0	37.0	41.0	34.0	41.0
21	38.255253329081384	40.0	37.0	41.0	34.0	41.0
22	38.2600355617402	40.0	37.0	41.0	34.0	41.0
23	38.21537924469956	40.0	37.0	41.0	34.0	41.0
24	38.16711118841924	40.0	37.0	41.0	34.0	41.0
25	38.067221835681565	40.0	37.0	41.0	34.0	41.0
26	38.003432964187006	40.0	37.0	41.0	34.0	41.0
27	37.933004049013356	40.0	37.0	41.0	34.0	41.0
28	37.90055637695445	40.0	37.0	41.0	34.0	41.0
29	37.8893853520419	40.0	36.0	41.0	34.0	41.0
30	37.54619402408148	39.0	36.0	41.0	33.0	41.0
31	37.58353022747917	39.0	36.0	41.0	33.0	41.0
32	37.46871557372297	39.0	36.0	41.0	33.0	41.0
33	37.302883593281926	39.0	36.0	41.0	33.0	41.0
34	37.32592624804314	39.0	36.0	41.0	33.0	41.0
35	37.257770675093255	39.0	36.0	41.0	32.0	41.0
36	37.070575075858606	39.0	35.0	41.0	32.0	41.0
37	37.00193391121161	39.0	35.0	41.0	31.0	41.0
38	36.94525980363735	39.0	35.0	41.0	31.0	41.0
39	36.885773612802225	39.0	35.0	41.0	31.0	41.0
40	36.86876944782667	39.0	35.0	41.0	31.0	41.0
41	36.80381974642933	39.0	35.0	41.0	31.0	41.0
42	36.731943719680714	39.0	35.0	40.0	31.0	41.0
43	36.6541620764964	39.0	35.0	40.0	31.0	41.0
44	36.538500657119116	39.0	35.0	40.0	31.0	41.0
45	36.53323524864228	39.0	35.0	40.0	31.0	41.0
46	36.39021037475116	39.0	35.0	40.0	30.0	41.0
47	36.26881728223266	39.0	35.0	40.0	30.0	41.0
48	36.25467834985795	39.0	35.0	40.0	30.0	41.0
49	36.253425716549735	39.0	35.0	40.0	30.0	41.0
50	36.15668063044781	39.0	35.0	40.0	30.0	41.0
51	35.06128360487814	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	12.0
11	4.0
12	2.0
13	3.0
14	6.0
15	8.0
16	30.0
17	47.0
18	92.0
19	202.0
20	406.0
21	651.0
22	1058.0
23	1756.0
24	2959.0
25	4765.0
26	6224.0
27	7256.0
28	8002.0
29	9392.0
30	11969.0
31	15878.0
32	22459.0
33	34153.0
34	50643.0
35	69166.0
36	85858.0
37	141166.0
38	188535.0
39	165081.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.19406273554821	4.783561392319437	56.032812469801506	14.989563402330841
2	20.828501575153165	4.765925474961829	52.72438684988694	21.68118609999807
3	19.134728745095767	4.724855530430413	53.43598403587097	22.70443168860285
4	18.794572969212037	4.099142846098839	53.675035271834716	23.43124891285441
5	16.705199938153495	7.398388125471096	51.80345374074719	24.092958195628224
6	14.549390232117663	29.936124156858195	42.200092769757056	13.31439284126708
7	78.91348253802593	1.6436433389381728	13.429388685955047	6.013485437080845
8	79.91377727527492	1.845006861096616	11.056149861811717	7.1850660018167405
9	74.58664308768674	4.0058899132216235	14.274704779575192	7.1327622195164375
10	36.79069789915154	24.671923619566687	22.268969482615333	16.268408998666434
11	24.200827197000443	26.323780947411144	28.436104829825474	21.039287025762935
12	22.772197097079687	22.529159660617307	33.305671517751875	21.39297172455113
13	22.027623161516015	26.130389826249978	33.76215187182312	18.079835140410893
14	19.238249164105838	27.997260393111844	31.516713051545196	21.247777391237125
15	16.574863261243504	26.917724821708124	35.10997081618059	21.397441100867784
16	16.764388975860538	26.771926518621598	32.45141667149842	24.012267834019443
17	17.399282000734427	26.690149011422275	29.63667594364237	26.273893044200925
18	18.784063895170174	24.702846871919753	33.83378268684409	22.679306546065984
19	19.541562783865793	27.28687114667285	31.105047254595004	22.066518814866352
20	20.769916507218646	27.929132602771496	31.171000753754274	20.12995013625558
21	20.346171314818037	28.704750584642742	31.4443574727972	19.504720627742024
22	19.143305115865562	26.20806033899615	29.63136100964419	25.017273535494095
23	17.843562165400744	26.757914419899116	28.761402949305193	26.637120465394947
24	19.833400977947854	25.433287914806442	29.82100751821573	24.912303589029978
25	18.440405094605826	27.164627664714637	27.23227227923697	27.16269496144257
26	19.09136371542877	27.101694014417966	29.849031715660697	23.957910554492567
27	19.85272801066852	25.79868962718154	32.039871668502734	22.308710693647203
28	19.4809242187047	23.692647030401425	32.48113198430645	24.345296766587428
29	21.838339034807987	22.025207282425928	31.53048356235867	24.605970120407413
30	22.009141686476873	25.97130418816799	30.231827757484393	21.78772636787074
31	22.235388763263174	28.590358709727298	26.003918555884116	23.170333971125412
32	21.86853752343403	31.36221758373437	24.35000773081309	22.419237162018515
33	20.08960495545119	30.059937960224964	26.329337469318336	23.52111961500551
34	19.769500976015152	26.44107187723469	27.007474729904718	26.78195241684544
35	21.074438066523644	26.476464505904406	26.09886260412439	26.350234823447554
36	22.42769273883381	28.252498018979143	28.291031290465973	21.02877795172107
37	21.041340522989504	26.988630873002066	30.312034943275158	21.65799366073327
38	21.676475135772407	29.75118861251232	26.410873388608646	22.161462863106625
39	21.4298138806749	28.705354554415262	27.82222995303531	22.04260161187453
40	20.701184747105778	27.206059991109566	26.780382095436888	25.31237316634777
41	17.925098084691058	28.761644537214202	25.577515896484414	27.735741481610326
42	21.055835797530005	28.597968728861055	26.48105467617557	23.86514079743337
43	23.10993699387333	25.548283759494407	28.692429601283315	22.64934964534895
44	22.049849249144778	23.637927369011035	27.149528420401616	27.16269496144257
45	23.760291644923758	22.66444888966197	25.39125161863899	28.184007846775284
46	23.77237104037417	23.692647030401425	29.193120542703078	23.34186138652133
47	19.333434800255116	25.93144218318162	30.83640149977774	23.89872151678553
48	19.947913646817806	24.633269554125356	30.742423803173498	24.676392995883344
49	23.355873485243812	22.784880462302624	31.255435727952687	22.603810324500877
50	22.331540751048493	21.90368856419474	28.015017104423954	27.749753580332808
51	19.697386985176166	22.706364391874914	25.425919483581687	32.17032913936723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	299.5
2	465.0
3	3209.5
4	5954.0
5	3932.0
6	1910.0
7	1983.5
8	2057.0
9	2290.0
10	2523.0
11	2687.0
12	2851.0
13	2919.0
14	2987.0
15	2851.0
16	2715.0
17	2663.0
18	2611.0
19	2547.0
20	2483.0
21	2714.0
22	2945.0
23	3049.0
24	3153.0
25	3506.0
26	4552.0
27	5245.0
28	6001.0
29	6757.0
30	7929.5
31	9102.0
32	10957.5
33	12813.0
34	15381.0
35	17949.0
36	19971.0
37	21993.0
38	24975.0
39	27957.0
40	33740.0
41	39523.0
42	45479.0
43	51435.0
44	60385.0
45	69335.0
46	87188.0
47	105041.0
48	120143.0
49	135245.0
50	123606.0
51	111967.0
52	89757.5
53	67548.0
54	53234.5
55	38921.0
56	31318.0
57	23715.0
58	19646.5
59	15578.0
60	13079.5
61	10581.0
62	9049.5
63	7518.0
64	6141.0
65	4764.0
66	4020.0
67	3276.0
68	2575.5
69	1875.0
70	1572.0
71	1269.0
72	996.0
73	723.0
74	614.0
75	392.0
76	279.0
77	230.5
78	182.0
79	127.5
80	73.0
81	51.0
82	29.0
83	18.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	827856.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.377853132426964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0816517973469	29.825840016133597
2	12.955569431155139	10.721472983385954
3	4.891821155413161	6.07239171956355
4	2.4872171514353525	4.116628240021815
5	1.4624737605865907	3.0257012237790053
6	0.9610972536631013	2.386088460483043
7	0.7273728488689742	2.1067988839110785
8	0.5684308567256516	1.8816358804426916
9	0.4492390957081156	1.672969439119924
>10	3.228023317364233	24.20747248595351
>50	0.11637288976028544	3.284131709314062
>100	0.06196470343745961	4.71144459615427
>500	0.003929468998459251	1.0947511162743833
>1k	0.004231735844494579	3.330905030117602
>5k	6.04533692070654E-4	1.5617682153453927
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	6389	0.7717525753271101	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	6098	0.736601534566398	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	4668	0.5638661796254422	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	3777	0.4562387661622311	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2970	0.35875804487736995	No Hit
CTGTCACTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	2002	0.24182949691733827	No Hit
CCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1796	0.21694594228948028	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	1675	0.2023298737944763	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	1562	0.1886801569355057	No Hit
TCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1468	0.1773255252121142	No Hit
GCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1280	0.15461626176533116	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	1199	0.1448319514504938	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC	1152	0.13915463558879806	No Hit
TGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	1058	0.12780000386540655	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.12345142150325661	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	1003	0.12115633636767748	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	931	0.11245917164337758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	929	0.11221758373436927	No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	904	0.10919773487176514	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8213988906283218	0.0
2	0.0	0.0	0.0	2.758933920875128	0.0
3	0.0	0.0	0.0	3.8054927426992133	0.0
4	0.0	0.0	0.0	4.94808275835411	0.0
5	0.0	0.0	0.0	6.832106066755571	0.0
6	0.0	0.0	0.0	7.9624958930055465	0.0
7	0.0	0.0	0.0	9.289900659051815	0.0
8	0.0	0.0	0.0	11.38893720647069	0.0
9	0.0	0.0	0.0	12.209490539417484	0.0
10	0.0	0.0	0.0	14.106680388859898	0.0
11	0.0	0.0	0.0	18.019921338976825	0.0
12	0.0	0.0	0.0	21.142565856863996	0.0
13	0.0	0.0	0.0	22.162912390560678	0.0
14	0.0	0.0	0.0	22.472507295954852	0.0
15	0.0	0.0	0.0	22.94287895479407	0.0
16	0.0	0.0	0.0	24.225227575810287	0.0
17	0.0	0.0	0.0	26.11166676330183	0.0
18	0.0	0.0	0.0	28.05185926054773	0.0
19	0.0	0.0	0.0	29.368392570688624	0.0
20	0.0	0.0	0.0	30.6641493206548	0.0
21	0.0	0.0	0.0	32.653746545292904	0.0
22	0.0	0.0	0.0	34.699029782957425	0.0
23	0.0	0.0	0.0	36.861845538354494	0.0
24	0.0	0.0	0.0	38.38227904369842	0.0
25	0.0	0.0	0.0	39.701107438974894	0.0
26	0.0	0.0	0.0	40.82412883400011	0.0
27	0.0	0.0	0.0	41.972637753425715	0.0
28	0.0	0.0	0.0	43.11945555748826	0.0
29	0.0	0.0	0.0	44.3301733634835	0.0
30	0.0	0.0	0.0	45.592107806188515	0.0
31	0.0	0.0	0.0	46.774197444966276	0.0
32	0.0	0.0	0.0	47.82305135192594	0.0
33	0.0	0.0	0.0	48.874079550066675	0.0
34	0.0	0.0	0.0	49.843451034962605	0.0
35	0.0	0.0	0.0	50.87152838174755	0.0
36	0.0	0.0	0.0	51.826404592102975	0.0
37	0.0	0.0	0.0	52.750599138014344	0.0
38	0.0	0.0	0.0	53.67274018669914	0.0
39	0.0	0.0	0.0	54.57277594170967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	20	7.0321414E-4	45.000004	20
GATCGCG	20	7.0321414E-4	45.000004	10
CGGCCAC	70	0.0	45.000004	6
CGTCTAC	20	7.0321414E-4	45.000004	20
TGACGCC	20	7.0321414E-4	45.000004	32
CTGCGCG	75	0.0	45.000004	1
CGTAGTT	20	7.0321414E-4	45.000004	24
TATAGCG	20	7.0321414E-4	45.000004	1
CCACGGT	20	7.0321414E-4	45.000004	37
TCGACCC	20	7.0321414E-4	45.000004	26
GAGATCG	20	7.0321414E-4	45.000004	9
ATAGCGG	25	3.889857E-5	45.0	2
TGTGCGA	25	3.889857E-5	45.0	17
CGTTCAT	25	3.889857E-5	45.0	17
GCGATAC	25	3.889857E-5	45.0	9
CCGAATC	50	2.1827873E-11	45.0	26
CTCGTAG	30	2.164763E-6	44.999996	1
CATGACG	30	2.164763E-6	44.999996	19
ACTTACG	30	2.164763E-6	44.999996	27
AACGCAT	30	2.164763E-6	44.999996	38
>>END_MODULE