##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	843764
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.365592748683284	33.0	31.0	34.0	31.0	34.0
2	32.531319184037244	34.0	31.0	34.0	31.0	34.0
3	32.60059329385942	34.0	31.0	34.0	31.0	34.0
4	36.06403804855386	37.0	35.0	37.0	35.0	37.0
5	35.21188626203536	37.0	35.0	37.0	35.0	37.0
6	26.430621595612042	37.0	0.0	37.0	0.0	37.0
7	31.277658207745294	37.0	19.0	37.0	17.0	37.0
8	34.82252975950621	37.0	32.0	37.0	32.0	37.0
9	37.31831886641288	39.0	37.0	39.0	35.0	39.0
10	37.373983720566414	39.0	37.0	39.0	34.0	39.0
11	37.42941509711246	39.0	37.0	39.0	35.0	39.0
12	37.07334870888068	39.0	37.0	39.0	34.0	39.0
13	37.01327503899194	39.0	37.0	39.0	33.0	39.0
14	37.95634205773178	40.0	37.0	41.0	33.0	41.0
15	38.1708404245737	40.0	37.0	41.0	33.0	41.0
16	38.30614247585818	40.0	37.0	41.0	34.0	41.0
17	38.33495977548225	40.0	37.0	41.0	34.0	41.0
18	38.349427090987525	40.0	37.0	41.0	34.0	41.0
19	38.36871210433249	40.0	37.0	41.0	34.0	41.0
20	38.272204076021254	40.0	37.0	41.0	34.0	41.0
21	38.17469576801096	40.0	37.0	41.0	34.0	41.0
22	38.22002716399372	40.0	37.0	41.0	34.0	41.0
23	38.17583115657933	40.0	36.0	41.0	34.0	41.0
24	38.126725008414674	40.0	36.0	41.0	34.0	41.0
25	38.00213685343295	40.0	36.0	41.0	34.0	41.0
26	37.98188000436141	40.0	36.0	41.0	34.0	41.0
27	37.95393498656022	40.0	36.0	41.0	34.0	41.0
28	37.86112704500311	40.0	36.0	41.0	34.0	41.0
29	37.85087180775667	40.0	36.0	41.0	34.0	41.0
30	37.56614645801432	40.0	35.0	41.0	33.0	41.0
31	37.52419752442626	40.0	35.0	41.0	33.0	41.0
32	37.51125433177997	40.0	35.0	41.0	33.0	41.0
33	37.46477214007708	40.0	35.0	41.0	33.0	41.0
34	37.35764976936679	40.0	35.0	41.0	33.0	41.0
35	37.35236393114663	40.0	35.0	41.0	33.0	41.0
36	37.25472051426702	40.0	35.0	41.0	32.0	41.0
37	37.13774467742165	39.0	35.0	41.0	32.0	41.0
38	37.0568180202047	39.0	35.0	41.0	32.0	41.0
39	36.996455169929035	39.0	35.0	41.0	32.0	41.0
40	36.87135028278049	39.0	35.0	41.0	31.0	41.0
41	36.78540681991647	39.0	35.0	41.0	31.0	41.0
42	36.797513285705485	39.0	35.0	41.0	31.0	41.0
43	36.73147586291901	39.0	35.0	41.0	31.0	41.0
44	36.58728625539843	39.0	35.0	41.0	31.0	41.0
45	36.51860946899844	39.0	35.0	41.0	31.0	41.0
46	36.41415016521207	39.0	35.0	40.0	31.0	41.0
47	36.315046624411565	38.0	35.0	40.0	31.0	41.0
48	36.271321127708696	38.0	35.0	40.0	31.0	41.0
49	36.209203047297585	38.0	35.0	40.0	31.0	41.0
50	36.0406144372123	38.0	35.0	40.0	30.0	41.0
51	34.965172726022914	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	9.0
11	5.0
12	10.0
13	12.0
14	11.0
15	20.0
16	46.0
17	79.0
18	154.0
19	249.0
20	483.0
21	787.0
22	1202.0
23	1937.0
24	2876.0
25	4114.0
26	5441.0
27	6675.0
28	7816.0
29	9596.0
30	12618.0
31	16708.0
32	24337.0
33	40263.0
34	59645.0
35	69421.0
36	77250.0
37	128994.0
38	181197.0
39	191679.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.688979382860612	4.426593218008827	51.43570951119033	17.44871788794023
2	19.717480243290776	4.604960628801418	52.732873173067354	22.944685954840452
3	21.521420681612394	4.754647034004769	51.637306166179165	22.086626118203668
4	19.84097449049734	4.816868223816138	51.13432191939926	24.207835366287256
5	18.264585832057307	8.126442939020864	50.23762568680342	23.371345542118412
6	16.42141641501652	30.548707932549863	39.60076514285985	13.429110509573766
7	84.19830663550472	2.2288222773192503	9.384614655282757	4.188256431893278
8	85.98044002825435	2.3738865369937563	7.114074551651884	4.531598883100014
9	81.1493498181956	4.528398936195429	9.36612607316738	4.956125172441583
10	41.84665380367022	23.604467599945007	18.70499333936978	15.843885257014994
11	33.08389549684509	24.362143917019452	23.438188877458625	19.115771708676835
12	28.809596048184087	22.2466234634329	29.25758861482595	19.68619187355706
13	25.78540919024751	24.018090366500587	31.004996657833235	19.19150378541867
14	21.22453671879815	24.991703841358483	33.20584902887537	20.577910410967995
15	19.77768665171778	23.23564409005362	35.80633921333454	21.18033004489407
16	23.882862980643875	23.284591426038563	30.882806092698907	21.949739500618655
17	24.439061159281504	23.851337577806117	27.57014994714162	24.13945131577076
18	25.579664455937916	22.657875898948046	30.843221564323674	20.919238080790365
19	25.96662099828862	24.509104441526304	27.691510896411792	21.83276366377328
20	25.18701911909017	27.760487529688398	28.137251648565236	18.915241702656193
21	24.15011780545271	26.720860335354434	30.36607392588449	18.762947933308364
22	22.863146567049554	22.75351875642952	30.67741690804538	23.705917768475544
23	21.16030074760241	25.433296514191174	29.959917702106274	23.446485036100142
24	22.337644175385535	25.781024077822707	30.017161196732733	21.86417055005902
25	23.438070360906604	26.343029567509397	26.2012837712915	24.0176163002925
26	22.170061770826912	25.41753381277229	27.19314879516073	25.219255621240062
27	22.681105143144293	24.867261461735744	30.316771040243477	22.134862354876482
28	18.880990419121936	25.909496020214185	29.715892121493688	25.493621439170195
29	21.511583807794597	25.63797459953257	28.6471098553624	24.20333173731043
30	24.373521506013528	25.506065677132465	28.51733423089869	21.603078585955316
31	23.488202862411764	27.693881227452227	27.39083440393285	21.427081506203155
32	24.583769869299946	26.2056688837163	26.52827093831925	22.682290308664506
33	24.39995069711436	25.450718447338357	25.764076210883612	24.38525464466367
34	20.29927799716508	26.182439639520055	29.755950716077006	23.762331647237854
35	21.817119478906424	26.294911847388608	27.688903532267318	24.199065141437654
36	25.466007082549147	27.20950407933972	25.663337141665206	21.661151696445927
37	21.3944894543972	29.123309361385413	26.519026647261555	22.96317453695583
38	20.91473445181354	28.193784043879567	26.906220222716303	23.985261281590585
39	20.38069886840396	26.65330590070209	26.728800944339888	26.23719428655406
40	23.282102578446107	25.87583731944003	27.868930174788208	22.97312992732565
41	20.86104645374773	24.768181624245642	26.95896008836594	27.411811833640687
42	21.560412627227517	23.467462465807976	29.10102824960534	25.871096657359168
43	21.996435022115186	23.535846516324472	28.32154488695891	26.146173574601427
44	22.32508142087124	24.010149757515133	27.749584006902406	25.915184814711225
45	21.76272038152848	24.42223180889443	25.938888125115554	27.876159684461534
46	22.170891386691068	24.60142883555117	28.33683352216971	24.890846255588055
47	19.669836589378072	25.884014961529527	30.541596939428562	23.90455150966384
48	20.078244627644697	26.568922115662673	28.797151810221816	24.555681446470814
49	22.53734456554202	24.346025665944506	29.152227400078694	23.964402368434776
50	21.440829426237666	25.15253080245187	26.531826434879896	26.874813336430563
51	19.69993979359157	25.421800408645073	25.898355464324148	28.97990433343921
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	530.5
2	903.0
3	2218.0
4	3533.0
5	2411.0
6	1289.0
7	1325.0
8	1361.0
9	1444.0
10	1527.0
11	1565.0
12	1603.0
13	1658.0
14	1713.0
15	1650.0
16	1587.0
17	1461.5
18	1336.0
19	1517.0
20	1698.0
21	1891.0
22	2084.0
23	2411.5
24	2739.0
25	3576.5
26	5130.0
27	5846.0
28	7038.5
29	8231.0
30	10199.5
31	12168.0
32	13735.5
33	15303.0
34	17994.5
35	20686.0
36	22658.0
37	24630.0
38	28092.0
39	31554.0
40	35800.5
41	40047.0
42	43039.5
43	46032.0
44	51594.5
45	57157.0
46	66075.5
47	74994.0
48	85220.5
49	95447.0
50	92458.0
51	89469.0
52	79330.0
53	69191.0
54	62705.5
55	56220.0
56	51335.0
57	46450.0
58	41681.0
59	36912.0
60	31614.0
61	26316.0
62	22979.0
63	19642.0
64	16767.5
65	13893.0
66	11753.0
67	9613.0
68	8130.5
69	6648.0
70	5537.5
71	4427.0
72	3594.0
73	2761.0
74	2394.0
75	1728.0
76	1429.0
77	981.0
78	533.0
79	321.0
80	109.0
81	75.5
82	42.0
83	29.5
84	17.0
85	15.5
86	14.0
87	10.5
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	843764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.39002470661901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35267759640337	24.538203454237326
2	10.882563943142221	7.485032857520444
3	4.060945006081866	4.1896799727412946
4	2.178487257815331	2.996729224772958
5	1.4525937153898862	2.4977366880468845
6	1.1207782584567905	2.3126155199382272
7	0.9034378973530722	2.174847612760766
8	0.7372836574823584	2.0284162557283785
9	0.6695710200146672	2.072390752902645
>10	6.484399504351002	42.54460075922485
>50	0.10932080152115765	2.5556162605779567
>100	0.04249346221692023	2.3851250266129975
>500	0.003268727862839838	0.7006991725648537
>1k	0.002179151908559892	1.5183064423704744
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	3357	0.39786006513669697	RNA PCR Primer, Index 36 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	2407	0.2852693407161244	RNA PCR Primer, Index 36 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2031	0.240707117155982	RNA PCR Primer, Index 15 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1806	0.21404089295110956	RNA PCR Primer, Index 15 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1465	0.17362674871172507	No Hit
CCTGTATCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1090	0.1291830417036043	RNA PCR Primer, Index 36 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30861710146439053	0.0
2	0.0	0.0	0.0	1.2459645114036626	0.0
3	0.0	0.0	0.0	1.7247713815711503	0.0
4	0.0	0.0	0.0	2.2039338013947027	0.0
5	0.0	0.0	0.0	3.0104389379020673	0.0
6	0.0	0.0	0.0	3.5285933033407444	0.0
7	0.0	0.0	0.0	4.199159954679271	0.0
8	0.0	0.0	0.0	5.259646062169042	0.0
9	0.0	0.0	0.0	5.738926998544617	0.0
10	0.0	0.0	0.0	6.755799014890419	0.0
11	0.0	0.0	0.0	8.556895055963516	0.0
12	0.0	0.0	0.0	10.065492246647167	0.0
13	0.0	0.0	0.0	10.589335406582883	0.0
14	0.0	0.0	0.0	10.780739638097856	0.0
15	0.0	0.0	0.0	11.058186886380552	0.0
16	0.0	0.0	0.0	11.730412769447382	0.0
17	0.0	0.0	0.0	12.631375597916005	0.0
18	0.0	0.0	0.0	13.59681143068441	0.0
19	0.0	0.0	0.0	14.243437738514562	0.0
20	0.0	0.0	0.0	14.97717371208063	0.0
21	0.0	0.0	0.0	15.975201596654989	0.0
22	0.0	0.0	0.0	17.00617708269137	0.0
23	0.0	0.0	0.0	18.01641217212396	0.0
24	0.0	0.0	0.0	18.816280381718112	0.0
25	0.0	0.0	0.0	19.49858017170678	0.0
26	1.1851655202165534E-4	0.0	0.0	20.11356256014715	0.0
27	2.370331040433107E-4	0.0	0.0	20.81660274673961	0.0
28	2.370331040433107E-4	0.0	0.0	21.48159912013312	0.0
29	2.370331040433107E-4	0.0	0.0	22.226001583381134	0.0
30	2.370331040433107E-4	0.0	0.0	23.08892059865081	0.0
31	2.370331040433107E-4	0.0	0.0	23.753916972044316	0.0
32	2.370331040433107E-4	0.0	0.0	24.401609928842664	0.0
33	2.370331040433107E-4	0.0	0.0	25.040295627687364	0.0
34	2.370331040433107E-4	0.0	0.0	25.74238768186365	0.0
35	2.370331040433107E-4	0.0	0.0	26.488093827183903	0.0
36	2.370331040433107E-4	0.0	0.0	27.13851266467875	0.0
37	2.370331040433107E-4	0.0	0.0	27.814294044306227	0.0
38	2.370331040433107E-4	0.0	0.0	28.564266785499264	0.0
39	2.370331040433107E-4	0.0	0.0	29.841638183188664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.88992E-5	45.000004	12
CTAGACG	25	3.88992E-5	45.000004	4
TCCGATA	20	7.0322177E-4	45.000004	14
CGGTTAT	25	3.88992E-5	45.000004	11
AACATCG	30	2.164812E-6	45.000004	37
TATAGCG	20	7.0322177E-4	45.000004	1
CACCGTA	25	3.88992E-5	45.000004	45
CGTTTCG	30	2.164812E-6	45.000004	42
CGAGGGT	110	0.0	42.954544	4
GTTTGCG	160	0.0	42.187504	1
GCGATGT	160	0.0	42.187504	9
CCGTTAG	70	0.0	41.785713	1
TCTTGCG	190	0.0	41.44737	1
CGTTAGG	250	0.0	41.4	2
TTTAGGG	3240	0.0	40.694447	3
TGTAGGG	990	0.0	40.681816	3
TTAGGGT	795	0.0	40.4717	4
TAGGGTG	435	0.0	40.34483	5
TAGGGCG	435	0.0	40.34483	5
TCGTTTG	190	0.0	40.263157	1
>>END_MODULE