##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299411
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.575582321528756	34.0	31.0	34.0	31.0	34.0
2	32.766551152791536	34.0	31.0	34.0	31.0	34.0
3	32.78254378329874	34.0	31.0	34.0	31.0	34.0
4	36.206617459756764	37.0	35.0	37.0	35.0	37.0
5	35.30428940496887	37.0	35.0	37.0	35.0	37.0
6	26.083516300847077	37.0	0.0	37.0	0.0	37.0
7	31.140193518447973	37.0	19.0	37.0	17.0	37.0
8	34.90272438820358	37.0	32.0	37.0	32.0	37.0
9	37.44936821375223	39.0	37.0	39.0	35.0	39.0
10	37.60346187618852	39.0	37.0	39.0	35.0	39.0
11	37.657230083476286	39.0	37.0	39.0	35.0	39.0
12	37.52601832676497	39.0	37.0	39.0	35.0	39.0
13	37.604459251153024	39.0	37.0	39.0	35.0	39.0
14	38.9274748328281	40.0	38.0	41.0	35.0	41.0
15	39.0150175733467	40.0	38.0	41.0	35.0	41.0
16	39.06254833920907	40.0	38.0	41.0	35.0	41.0
17	39.03602863143378	40.0	38.0	41.0	35.0	41.0
18	38.9859459401221	40.0	38.0	41.0	35.0	41.0
19	38.95383600723712	40.0	38.0	41.0	35.0	41.0
20	38.87865271265212	40.0	38.0	41.0	35.0	41.0
21	38.77628864154605	40.0	38.0	41.0	35.0	41.0
22	38.74244407658547	40.0	38.0	41.0	35.0	41.0
23	38.682493837592574	40.0	38.0	41.0	35.0	41.0
24	38.6418769734903	40.0	38.0	41.0	35.0	41.0
25	38.547538846446585	40.0	38.0	41.0	35.0	41.0
26	38.51015113770778	40.0	38.0	41.0	35.0	41.0
27	38.491368012122415	40.0	38.0	41.0	35.0	41.0
28	38.43327553791679	40.0	37.0	41.0	35.0	41.0
29	38.416631073617204	40.0	37.0	41.0	35.0	41.0
30	38.19311826666082	40.0	37.0	41.0	34.0	41.0
31	38.18880323469634	40.0	37.0	41.0	34.0	41.0
32	38.082381940740845	40.0	37.0	41.0	34.0	41.0
33	37.95810871233197	40.0	37.0	41.0	34.0	41.0
34	37.83482208477533	40.0	37.0	41.0	34.0	41.0
35	37.76866133963773	40.0	37.0	41.0	34.0	41.0
36	37.6974252180411	40.0	37.0	41.0	33.0	41.0
37	37.65071020639351	40.0	37.0	41.0	33.0	41.0
38	37.566150355815054	40.0	37.0	41.0	33.0	41.0
39	37.51215204427237	40.0	37.0	41.0	33.0	41.0
40	37.43155167995346	40.0	36.0	41.0	33.0	41.0
41	37.33889200568565	40.0	36.0	41.0	33.0	41.0
42	37.30030529216699	40.0	36.0	41.0	33.0	41.0
43	37.232695428928956	40.0	36.0	41.0	33.0	41.0
44	37.10366773869084	40.0	36.0	41.0	32.0	41.0
45	37.05686499498619	40.0	35.0	41.0	32.0	41.0
46	36.989368259927	40.0	35.0	41.0	32.0	41.0
47	36.937073797282	39.0	35.0	41.0	32.0	41.0
48	36.904933081219106	39.0	35.0	41.0	32.0	41.0
49	36.86746456663827	39.0	35.0	41.0	32.0	41.0
50	36.775031148728154	39.0	35.0	41.0	32.0	41.0
51	35.87786004582076	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	15.0
11	12.0
12	17.0
13	12.0
14	8.0
15	20.0
16	29.0
17	84.0
18	149.0
19	235.0
20	421.0
21	809.0
22	1381.0
23	2390.0
24	4259.0
25	7245.0
26	9927.0
27	10856.0
28	11075.0
29	11234.0
30	13279.0
31	17128.0
32	23691.0
33	39644.0
34	62914.0
35	90694.0
36	108655.0
37	193952.0
38	307298.0
39	381685.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.80741889979383	4.285018366013524	56.20838980122532	17.699172932967322
2	21.596785004898372	3.969798624145863	52.87857344596898	21.554842924986783
3	19.26357403469726	3.9115414599383875	53.99661846790585	22.828266037458512
4	18.085501815822706	4.551600686772699	53.170628846454285	24.192268650950314
5	17.30661045658379	7.82531470027574	50.77092621195295	24.097148631187515
6	15.618076189904503	30.99527401261033	40.306261837093885	13.080387960391285
7	83.47027999609054	1.6477465559395756	10.636665381468989	4.245308066500899
8	85.65111423560367	2.0975657432482873	8.50639251168414	3.744927509463903
9	80.00301675143584	4.896295321495662	10.54408497388432	4.556602953184173
10	37.260497256064475	31.747460965006454	17.416737275581013	13.575304503348054
11	25.04765620731239	24.478321331741842	31.030059003656273	19.443963457289495
12	24.829172602048157	21.4966627187241	33.07898732579607	20.59517735343167
13	23.170805849727298	22.600393562929668	34.066588631310644	20.162211956032387
14	19.297204656571324	24.726202871916584	33.72266357603561	22.253928895476488
15	17.75750705511959	26.003781713407076	35.71079512178979	20.52791610968354
16	20.196843031188745	24.950612238929793	34.408897569745065	20.4436471601364
17	20.257331975795186	24.807701335451217	31.71929435721261	23.215672331540983
18	20.398088056819592	24.73566869912599	32.80817231807334	22.05807092598108
19	22.105553977917687	26.9547510372007	30.751317327619976	20.188377657261636
20	24.12031297256988	26.01086184432793	30.530294110177614	19.338531072924578
21	23.872662306229515	26.38757098408433	30.462417202871144	19.27734950681501
22	22.14041592690842	24.48193835514706	30.628800279511253	22.74884543843326
23	21.15981779436991	25.916819235792214	30.538220778491176	22.385142191346695
24	21.10294587316869	24.23036283362231	32.44739347288887	22.21929782032013
25	20.496902057932402	26.108367560379282	30.18406031655881	23.21067006512951
26	20.118884633114543	27.17523554902952	30.401004762927204	22.304875054928733
27	20.17883487210744	26.37602729236554	30.894843894656887	22.550293940870134
28	18.374709772350702	26.4211246480136	32.1256323057139	23.0785332739218
29	19.94565229938795	24.26784135273597	31.724450539513672	24.062055808362402
30	21.04976793331748	24.10784578551359	31.494346284585863	23.34803999658307
31	20.459346580873948	26.10636665381469	29.836979985547295	23.597306779764065
32	21.03399155463514	25.871183174530614	30.02883614191353	23.06598912892072
33	21.12872678467398	25.009562024640395	29.915169257455876	23.94654193322975
34	18.9939903540912	24.363730951946692	31.796329259949314	24.845949434012795
35	19.74971737194775	23.652716500014236	32.10924026347322	24.488325864564793
36	19.088956457964414	25.76936781357092	30.58770473699238	24.55397099147229
37	19.185538678678263	26.211568164345227	31.05268463942509	23.550208517551415
38	20.227164461436757	25.071282296363506	30.70167945322919	23.99987378897054
39	20.717540485650808	24.421295494651037	30.885839815116235	23.97532420458192
40	20.72084967727686	23.873970591290977	32.89921356676217	22.505966164669992
41	17.47707230429787	24.827864316986698	33.99709560716355	23.69796777155188
42	19.29227934810464	24.891816369108774	31.827189395810873	23.988714886975714
43	19.7701881852624	24.342952306852876	31.670964767883298	24.21589474000143
44	20.182759727291828	24.898280836471294	29.577785627488147	25.341173808748735
45	20.804041215596914	23.240683663598354	29.6210359924612	26.334239128343533
46	19.75926015710195	24.516569430303424	30.314350117091514	25.409820295503117
47	18.492301511992743	24.74167141881976	32.30563693858217	24.46039013060533
48	18.78150946852074	22.93708457139427	33.07252285843355	25.20888310165144
49	19.794506895816642	22.69482096118934	32.010964967973955	25.499707175020063
50	19.397480858635184	22.647183993363146	31.665423795858278	26.289911352143395
51	17.87117393957724	22.58569459547441	30.818578571368104	28.724552893580245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	235.0
1	853.0
2	1471.0
3	8989.0
4	16507.0
5	10807.0
6	5107.0
7	4810.0
8	4513.0
9	4488.5
10	4464.0
11	4483.0
12	4502.0
13	4437.0
14	4372.0
15	4334.5
16	4297.0
17	4304.5
18	4312.0
19	4578.0
20	4844.0
21	5533.0
22	6222.0
23	6624.0
24	7026.0
25	8901.5
26	13140.0
27	15503.0
28	17765.5
29	20028.0
30	23048.0
31	26068.0
32	30574.5
33	35081.0
34	39213.5
35	43346.0
36	46158.0
37	48970.0
38	53393.0
39	57816.0
40	62350.0
41	66884.0
42	72868.0
43	78852.0
44	85685.0
45	92518.0
46	102530.0
47	112542.0
48	120700.5
49	128859.0
50	122914.0
51	116969.0
52	102228.5
53	87488.0
54	77005.0
55	66522.0
56	59971.5
57	53421.0
58	50678.0
59	47935.0
60	43835.5
61	39736.0
62	35085.5
63	30435.0
64	25075.0
65	19715.0
66	15691.5
67	11668.0
68	9858.0
69	8048.0
70	6668.0
71	5288.0
72	4308.5
73	3329.0
74	2536.5
75	1436.5
76	1129.0
77	771.5
78	414.0
79	371.0
80	328.0
81	195.0
82	62.0
83	53.0
84	44.0
85	25.0
86	6.0
87	4.5
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1299411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.00026378744351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42065647848878	26.43163000672869
2	10.104823643411654	7.27552633376831
3	3.512702769048747	3.7937467887791447
4	1.8810249175074623	2.708695728840912
5	1.359955080049122	2.447937081042112
6	1.0387498178011105	2.243716046999932
7	0.8680035076923718	2.1873848671746146
8	0.7417662726519152	2.136302518727815
9	0.6584567151115196	2.1334153892965424
>10	6.272539754523498	41.853212611429846
>50	0.10491533431982625	2.578491208384958
>100	0.032082531645257935	1.9460996287308605
>500	0.0027304280518601768	0.6612872153796214
>1k	0.0013652140259300884	1.0137607553001604
>5k	2.2753567098834806E-4	0.5887938194164151
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7188	0.553173707164246	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2749	0.21155739023295939	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2573	0.19801279194958332	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	2369	0.18231337121203375	TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1782	0.13713905761918285	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1500	0.11543691718786434	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	1403	0.10797199654304912	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.695794479190956E-5	0.0	0.0	0.19100961897351953	0.0
2	7.695794479190956E-5	0.0	0.0	0.6738437645979601	0.0
3	7.695794479190956E-5	0.0	0.0	1.038932254690779	0.0
4	7.695794479190956E-5	0.0	0.0	1.4361122077618245	0.0
5	7.695794479190956E-5	0.0	0.0	2.117113061225432	0.0
6	7.695794479190956E-5	0.0	0.0	2.8182768962245204	0.0
7	7.695794479190956E-5	0.0	0.0	3.551224362422667	0.0
8	7.695794479190956E-5	0.0	0.0	4.665960192733477	0.0
9	7.695794479190956E-5	0.0	0.0	5.2475313815259375	0.0
10	7.695794479190956E-5	0.0	0.0	6.180569504183049	0.0
11	1.5391588958381912E-4	0.0	0.0	7.8912676589624065	0.0
12	1.5391588958381912E-4	0.0	0.0	9.245342697576056	0.0
13	1.5391588958381912E-4	0.0	0.0	9.757497820166213	0.0
14	1.5391588958381912E-4	0.0	0.0	9.941889055887629	0.0
15	1.5391588958381912E-4	0.0	0.0	10.191848460571752	0.0
16	1.5391588958381912E-4	0.0	0.0	10.765723854885021	0.0
17	1.5391588958381912E-4	0.0	0.0	11.601025387656408	0.0
18	1.5391588958381912E-4	0.0	0.0	12.465109191779968	0.0
19	1.5391588958381912E-4	0.0	0.0	13.084004983796504	0.0
20	1.5391588958381912E-4	0.0	0.0	13.704516892653672	0.0
21	1.5391588958381912E-4	0.0	0.0	14.625857407702412	0.0
22	1.5391588958381912E-4	0.0	0.0	15.63323690502851	0.0
23	1.5391588958381912E-4	0.0	0.0	16.671245664381786	0.0
24	1.5391588958381912E-4	0.0	0.0	17.4073484063164	0.0
25	1.5391588958381912E-4	0.0	0.0	18.057566081863243	0.0
26	1.5391588958381912E-4	0.0	0.0	18.649218761423445	0.0
27	1.5391588958381912E-4	0.0	0.0	19.211396548128345	0.0
28	1.5391588958381912E-4	0.0	0.0	19.81897952226047	0.0
29	1.5391588958381912E-4	0.0	0.0	20.4674271650771	0.0
30	1.5391588958381912E-4	0.0	0.0	21.172669771150158	0.0
31	2.308738343757287E-4	0.0	0.0	21.83727858237309	0.0
32	2.308738343757287E-4	0.0	0.0	22.460560977242768	0.0
33	2.308738343757287E-4	0.0	0.0	23.065681297141552	0.0
34	2.308738343757287E-4	0.0	0.0	23.645405495259006	0.0
35	2.308738343757287E-4	0.0	0.0	24.286388217430822	0.0
36	2.308738343757287E-4	0.0	0.0	24.906746210398403	0.0
37	2.308738343757287E-4	0.0	0.0	25.51956232477638	0.0
38	2.308738343757287E-4	0.0	0.0	26.12252782222099	0.0
39	2.308738343757287E-4	0.0	0.0	26.732958240310417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	20	7.0336263E-4	45.000004	1
CGTTTAT	1475	0.0	43.169495	1
CGTTTTT	3155	0.0	42.86054	1
CGTTAGG	375	0.0	42.600002	2
TAGGGAT	2240	0.0	42.589287	5
TTAGGGA	3700	0.0	42.26351	4
TCGTTAG	220	0.0	40.909092	1
TGTAGGG	1700	0.0	40.897057	3
TAGGGAA	1435	0.0	40.766552	5
TTTAGGG	5245	0.0	40.753098	3
CCGTAGG	100	0.0	40.499996	2
TTTTAGG	2905	0.0	40.430294	2
AGGGATC	935	0.0	40.427807	6
CTAGGGA	1765	0.0	40.410767	4
TAGGGAC	1465	0.0	40.392494	5
TAGGGCG	535	0.0	40.373833	5
AGGGATA	865	0.0	40.057804	6
CGTTCGG	90	0.0	40.0	2
TATTGCG	45	1.9286745E-8	40.0	1
TGCGTAG	90	0.0	40.0	1
>>END_MODULE