##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932071
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34782221526043	33.0	31.0	34.0	31.0	34.0
2	32.50842156874315	34.0	31.0	34.0	31.0	34.0
3	32.56960682179791	34.0	31.0	34.0	31.0	34.0
4	36.051106621705856	37.0	35.0	37.0	35.0	37.0
5	35.13308535508561	37.0	35.0	37.0	35.0	37.0
6	25.93786310270355	36.0	0.0	37.0	0.0	37.0
7	31.022827660124605	37.0	19.0	37.0	17.0	37.0
8	34.730607432266424	37.0	32.0	37.0	32.0	37.0
9	37.24266284435413	39.0	37.0	39.0	35.0	39.0
10	37.38917314238937	39.0	37.0	39.0	35.0	39.0
11	37.43043394762845	39.0	37.0	39.0	35.0	39.0
12	37.03247713961704	39.0	35.0	39.0	34.0	39.0
13	36.911553948143435	39.0	35.0	39.0	33.0	39.0
14	37.6397946079215	40.0	37.0	41.0	32.0	41.0
15	37.899118200222944	40.0	37.0	41.0	33.0	41.0
16	38.14312858140635	40.0	37.0	41.0	33.0	41.0
17	38.213168310139466	40.0	37.0	41.0	34.0	41.0
18	38.2167442179834	40.0	37.0	41.0	34.0	41.0
19	38.23144588770598	40.0	37.0	41.0	34.0	41.0
20	38.10492011874632	40.0	36.0	41.0	33.0	41.0
21	38.01421136372658	40.0	36.0	41.0	33.0	41.0
22	38.0860610404143	40.0	36.0	41.0	34.0	41.0
23	38.070538617766246	40.0	36.0	41.0	34.0	41.0
24	38.009390915498926	40.0	36.0	41.0	34.0	41.0
25	37.89454451431275	40.0	35.0	41.0	34.0	41.0
26	37.85845069742541	40.0	35.0	41.0	34.0	41.0
27	37.82672993795537	40.0	35.0	41.0	34.0	41.0
28	37.76590731821932	40.0	35.0	41.0	34.0	41.0
29	37.73130909555174	40.0	35.0	41.0	34.0	41.0
30	37.48253620164129	40.0	35.0	41.0	33.0	41.0
31	37.475154789710224	40.0	35.0	41.0	33.0	41.0
32	37.45543418902637	40.0	35.0	41.0	33.0	41.0
33	37.356580131771075	39.0	35.0	41.0	33.0	41.0
34	37.17948096228721	39.0	35.0	41.0	32.0	41.0
35	37.15414598244125	39.0	35.0	41.0	32.0	41.0
36	37.09289313796911	39.0	35.0	41.0	32.0	41.0
37	36.98525541509177	39.0	35.0	41.0	32.0	41.0
38	36.925765311870016	39.0	35.0	41.0	32.0	41.0
39	36.87820241161886	39.0	35.0	41.0	32.0	41.0
40	36.73942221139806	39.0	35.0	41.0	31.0	41.0
41	36.62525816166365	39.0	35.0	41.0	31.0	41.0
42	36.636587770674126	39.0	35.0	41.0	31.0	41.0
43	36.55182598750525	39.0	35.0	41.0	31.0	41.0
44	36.43121714976649	39.0	35.0	40.0	31.0	41.0
45	36.467233719319665	39.0	35.0	40.0	31.0	41.0
46	36.46828192272906	38.0	35.0	40.0	31.0	41.0
47	36.39143262691361	38.0	35.0	40.0	31.0	41.0
48	36.33739382514851	38.0	35.0	40.0	31.0	41.0
49	36.32266747919418	38.0	35.0	40.0	31.0	41.0
50	36.20266696421196	38.0	35.0	40.0	31.0	41.0
51	35.18896629119455	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	18.0
11	13.0
12	8.0
13	9.0
14	23.0
15	29.0
16	58.0
17	86.0
18	184.0
19	311.0
20	521.0
21	918.0
22	1389.0
23	2101.0
24	3121.0
25	4657.0
26	6211.0
27	7635.0
28	8970.0
29	11036.0
30	14368.0
31	20033.0
32	28915.0
33	48774.0
34	71038.0
35	76679.0
36	85097.0
37	141104.0
38	195090.0
39	203521.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.505567708897715	4.405672958390508	53.5879777398932	16.500781592818573
2	21.625927638559723	4.630441243210013	51.77642046582288	21.96721065240738
3	21.742013215731422	4.757148328829027	50.89215306559264	22.60868538984691
4	20.120570214071677	5.015283170488085	49.17801326293812	25.686133352502118
5	18.013971038686968	8.132749543757932	48.83812499262395	25.015154424931147
6	16.099846470923353	31.78277191329845	39.172337729636475	12.945043886141722
7	82.93928252246879	2.4986293962584396	9.863840844742516	4.698247236530265
8	84.04928379919555	2.6933570511259335	8.107966024047524	5.149393125630987
9	78.06100608215468	5.123751302207665	10.719784222446574	6.095458393191076
10	43.24016088903099	24.00804230579001	18.21567241122189	14.536124393957111
11	33.927458315943746	22.818111495798064	24.95807722802233	18.29635296023586
12	31.85143621033162	19.96156945125425	28.92547885300583	19.2615154854083
13	28.412535096575258	21.54953860811033	31.27057917261668	18.767347122697732
14	21.106868468174635	23.776085727374845	34.83962058684371	20.27742521760681
15	18.836762435479702	22.969387525199263	38.40866200107073	19.785188038250304
16	24.331944669451147	22.493136252495784	32.02878321501259	21.146135863040477
17	25.537432234239667	22.422326196180332	28.453089947010472	23.587151622569525
18	25.436366972043977	21.870007756919804	31.19472658198785	21.498898689048364
19	26.307759816580496	24.11704687733016	27.89433422990309	21.680859076186255
20	25.019124079603376	28.271022271908468	28.60790647922744	18.10194716926071
21	24.361878011439043	25.209452927942184	32.09594548054815	18.332723580070617
22	21.51509917162963	22.597849305471364	32.82646922820257	23.060582294696435
23	21.63451067568887	24.43655043446261	31.501570159354813	22.427368730493708
24	22.718440977135863	25.54161646484012	28.774310111568752	22.96563244645526
25	23.650773385289316	24.949065039036725	26.944406595634884	24.455754980039075
26	20.00051498222775	25.630987338947353	28.034237735108164	26.33425994371673
27	19.884751268948396	25.785482007271977	31.733741313698204	22.59602541008142
28	17.45371329008198	25.250222354305627	30.681353673700823	26.614710681911568
29	19.78454431046562	26.59336037705282	30.266363828506627	23.355731483974935
30	23.646374578760632	24.97524330228062	29.44646920674498	21.931912912213768
31	22.85544770730985	25.342168139551603	29.538951431811526	22.26343272132702
32	27.208120411427885	25.07040772645002	26.78454752910454	20.936924333017547
33	27.139670690322948	24.935117603701865	25.764346278341456	22.160865427633734
34	22.029223095665458	23.91942244743158	30.76814963666931	23.283204820233653
35	21.34118538180031	26.570508040696474	29.46824866346019	22.62005791404303
36	25.463510826964896	26.55881365260801	26.31709386945844	21.66058165096865
37	22.26504204078874	28.589131085507436	27.242345271980355	21.903481601723474
38	23.469027574079657	25.813376877941703	27.408748904321666	23.308846643656974
39	22.747086863554387	24.141293957219997	28.492250053912198	24.619369125313415
40	26.7512882602291	23.2970449676044	28.464247895278366	21.487418876888135
41	23.723407336994715	22.926472339553534	27.0816279017371	26.268492421714658
42	23.20595748607134	22.909413553259355	30.10081849987823	23.783810460791077
43	22.617268426976057	22.278667612231256	30.48469483547927	24.619369125313415
44	22.448182595531886	22.69902185563117	29.016459046574777	25.83633650226217
45	21.52185831336883	22.692691865748422	27.246636790544926	28.538813030337817
46	21.944787467907485	23.60860921539239	29.534230761390496	24.912372555309627
47	19.486069194299578	24.338596523226236	32.38497925587214	23.79035502660205
48	20.47226016043842	25.84567055514011	29.9313035165776	23.750765767843866
49	23.908371787127805	22.595810834153195	30.626958675894862	22.86885870282414
50	21.66712621677962	23.483082297378633	28.298380702757626	26.551410783084123
51	20.46442813905808	24.093336237260896	26.43328673459425	29.008948889086778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	566.5
2	988.0
3	2968.0
4	4948.0
5	3310.0
6	1672.0
7	1629.5
8	1587.0
9	1763.5
10	1940.0
11	1905.0
12	1870.0
13	1846.5
14	1823.0
15	1807.0
16	1791.0
17	1945.5
18	2100.0
19	1898.5
20	1697.0
21	2031.5
22	2366.0
23	2868.5
24	3371.0
25	4238.5
26	5887.0
27	6668.0
28	8123.5
29	9579.0
30	11055.0
31	12531.0
32	14936.0
33	17341.0
34	19726.0
35	22111.0
36	24162.5
37	26214.0
38	29276.5
39	32339.0
40	36906.0
41	41473.0
42	46443.0
43	51413.0
44	58305.0
45	65197.0
46	73655.0
47	82113.0
48	93813.5
49	105514.0
50	103642.0
51	101770.0
52	88651.5
53	75533.0
54	66800.0
55	58067.0
56	53458.5
57	48850.0
58	45528.0
59	42206.0
60	37110.5
61	32015.0
62	27843.0
63	23671.0
64	19930.5
65	16190.0
66	13743.0
67	11296.0
68	9531.0
69	7766.0
70	6240.0
71	4714.0
72	3633.5
73	2553.0
74	2169.0
75	1318.0
76	851.0
77	664.0
78	477.0
79	370.0
80	263.0
81	194.5
82	126.0
83	69.5
84	13.0
85	10.5
86	8.0
87	12.0
88	16.0
89	8.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	932071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.01388546610228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.51555533643918	23.279924674558007
2	11.361563408294224	7.501787061545684
3	4.429521726256887	4.387071688207696
4	2.298536541408471	3.0353448847084046
5	1.468560481764331	2.4241443772505806
6	1.100318497117405	2.1795473304040676
7	0.8632755086647327	1.9950055138124072
8	0.7357714829913113	1.9432540374959502
9	0.6410006630186011	1.9045730225332551
>10	6.380819939449712	42.426002711908936
>50	0.14184454397024535	3.1466421443846473
>100	0.05707958051006676	3.29716858624547
>500	0.004101526743235755	0.9945668692387676
>1k	0.0020507633716178777	1.48496709770617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	2810	0.30147917916124417	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	2630	0.28216734562066625	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	2246	0.24096876740076667	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2191	0.23506792937447898	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	2173	0.2331367460204212	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1110	0.11908964016689716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2991188439507291	0.0
2	0.0	0.0	0.0	1.1458354567409563	0.0
3	0.0	0.0	0.0	1.6302406147171191	0.0
4	0.0	0.0	0.0	2.1577755342672393	0.0
5	0.0	0.0	0.0	3.0769115228346338	0.0
6	0.0	0.0	0.0	3.676543954269578	0.0
7	0.0	0.0	0.0	4.442043578225264	0.0
8	0.0	0.0	0.0	5.6361586188176656	0.0
9	0.0	0.0	0.0	6.230319364082779	0.0
10	0.0	0.0	0.0	7.378944307890708	0.0
11	0.0	0.0	0.0	9.43254322900294	0.0
12	0.0	0.0	0.0	11.210197506413138	0.0
13	0.0	0.0	0.0	11.808435194314596	0.0
14	0.0	0.0	0.0	12.049725825607705	0.0
15	0.0	0.0	0.0	12.37577394855113	0.0
16	0.0	0.0	0.0	13.09792923500463	0.0
17	0.0	0.0	0.0	14.058263801791924	0.0
18	0.0	0.0	0.0	15.094450959208043	0.0
19	0.0	0.0	0.0	15.80984710392234	0.0
20	0.0	0.0	0.0	16.63403324424856	0.0
21	0.0	0.0	0.0	17.715495922520923	0.0
22	0.0	0.0	0.0	18.90446114083584	0.0
23	0.0	0.0	0.0	19.92434052770658	0.0
24	0.0	0.0	0.0	20.705933346279416	0.0
25	0.0	0.0	0.0	21.391825300862273	0.0
26	0.0	0.0	0.0	22.041346635610378	0.0
27	0.0	0.0	0.0	22.81918437543921	0.0
28	0.0	0.0	0.0	23.46688181479737	0.0
29	0.0	0.0	0.0	24.252980727863005	0.0
30	0.0	0.0	0.0	25.17705196277966	0.0
31	0.0	0.0	0.0	25.89706148995087	0.0
32	0.0	0.0	0.0	26.58488462788779	0.0
33	0.0	0.0	0.0	27.262300833305616	0.0
34	0.0	0.0	0.0	28.0165352210293	0.0
35	0.0	0.0	0.0	28.830636292728773	0.0
36	0.0	0.0	0.0	29.522643661266148	0.0
37	0.0	0.0	0.0	30.254240288561707	0.0
38	0.0	0.0	0.0	31.10524841991651	0.0
39	0.0	0.0	0.0	32.83827090425515	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTAT	30	2.1650576E-6	45.000004	18
ACTCGCC	30	2.1650576E-6	45.000004	40
GTCGACA	40	6.8121153E-9	45.0	32
ACGTCGA	40	6.8121153E-9	45.0	30
CATCGAA	25	3.8902363E-5	45.0	21
GGCGATA	40	6.8121153E-9	45.0	8
TTTAGGG	3440	0.0	41.664246	3
TTAGGGA	1830	0.0	41.434425	4
CTTTAGG	925	0.0	41.351353	2
TAGGGAT	980	0.0	41.09694	5
ACGGGTA	55	6.002665E-11	40.909092	5
TTTTAGG	1885	0.0	40.82228	2
CGTAGGG	355	0.0	40.56338	3
GCGTTAG	100	0.0	40.5	1
GATTCGT	50	1.0804797E-9	40.5	11
GTTAGGG	1445	0.0	40.48443	3
TAGGGGT	695	0.0	40.46763	5
CTAGGGA	990	0.0	40.454544	4
CTTAGGG	1460	0.0	40.376713	3
TAGGGAG	1020	0.0	40.14706	5
>>END_MODULE