FastQCFastQC Report
Sat 14 Jan 2017
SRR2934285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797288
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC78530.9849640280551069No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG66640.8358334754818836No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC46490.5831017148132168No Hit
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC26800.3361395129488967No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC23530.2951254753614754No Hit
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT22680.2844643340925738No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.26351832712896717No Hit
TCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC17930.22488736817812385No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT16220.20343966044892187No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTC14740.1848767321218932No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGCTTCGT13610.17070368549382406No Hit
GAATCAGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC13570.1702019847282287No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTA12720.15954084345932712No Hit
GAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT10330.12956422271500387No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8740.10962161728258799No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC253.889727E-545.09
CCGATAG406.8102963E-945.011
CCGTAGG406.8102963E-945.02
CACTCGA207.031985E-445.026
ACGACCA700.045.028
GTACGCA253.889727E-545.010
ACGAACG302.164661E-644.99999621
CGTTAGG1650.043.6363642
CTAGGGA8950.042.234644
GATCCGG750.042.0000045
CGTTTAT4500.042.0000041
GTTAGGG10500.042.03
TTAGGGA14150.041.5017664
TCGTTAG1250.041.3999981
TAGGGAG8650.041.358385
TTTCGCG1200.041.2499961
TCTAGGG13400.040.9701463
TTTAGGG27650.040.9312823
TCGTTGA556.002665E-1140.90909224
TAGGGAC5500.040.9090925
Produced by FastQC (version 0.11.5)