FastQCFastQC Report
Sat 14 Jan 2017
SRR2934284.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934284.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669929
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT53020.7914271512354295TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC42800.6388736716875967TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC41560.6203642475545915TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC27590.41183468695936437TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT15260.22778533247553098TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG14120.21076860383712304No Hit
TCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC13910.20763394329846893TruSeq Adapter, Index 23 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG12730.1900201364622221No Hit
GCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC12270.18315373718707503TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC11910.17778003340652518TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.16957020818624063No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC10980.1638979653067713No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT9230.13777579415132052No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC8190.12225176100750974No Hit
TTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG7780.11613170947966128No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG7280.10866823200667533No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG7250.10822042335829618No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG207.031176E-445.00000427
CGTCTCG207.031176E-445.00000442
GCGTTAG302.1641408E-645.0000041
CCTCGAC207.031176E-445.00000426
TCGGATC207.031176E-445.00000438
CCTCCGA207.031176E-445.00000430
AAACCGC207.031176E-445.00000426
CGTGTGA207.031176E-445.00000418
CCGTTAG302.1641408E-645.0000041
TCGTTGA351.2109194E-745.024
TCGTTAG900.045.01
CGTTGAT351.2109194E-745.025
CGTTAGG1500.045.02
CGTAAGG253.8890576E-545.02
TAGGGCG4050.043.888895
AGGGCGA5650.043.8053056
AGGGCGC900.042.56
TTAGGGA12550.042.1314744
CTAGGGA8500.042.0882384
CGTTTTT7700.042.077921
Produced by FastQC (version 0.11.5)