##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669929
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23582349771394	33.0	31.0	34.0	30.0	34.0
2	32.40596540827461	34.0	31.0	34.0	30.0	34.0
3	32.464532808700625	34.0	31.0	34.0	30.0	34.0
4	35.965800853523284	37.0	35.0	37.0	35.0	37.0
5	35.060321317632166	37.0	35.0	37.0	35.0	37.0
6	25.82957298459986	36.0	0.0	37.0	0.0	37.0
7	30.93861588317568	37.0	19.0	37.0	17.0	37.0
8	34.64940165301099	37.0	32.0	37.0	32.0	37.0
9	37.17007324656792	39.0	37.0	39.0	34.0	39.0
10	37.24082850570732	39.0	37.0	39.0	34.0	39.0
11	37.33226953901085	39.0	37.0	39.0	34.0	39.0
12	37.14331518713177	39.0	37.0	39.0	34.0	39.0
13	37.165562320783245	39.0	37.0	39.0	34.0	39.0
14	38.21045663047875	40.0	38.0	41.0	33.0	41.0
15	38.34925044295739	40.0	38.0	41.0	34.0	41.0
16	38.432956328207915	40.0	38.0	41.0	34.0	41.0
17	38.44337235736921	40.0	38.0	41.0	34.0	41.0
18	38.456575249018925	40.0	38.0	41.0	34.0	41.0
19	38.471366368674886	40.0	38.0	41.0	34.0	41.0
20	38.40819250995255	40.0	38.0	41.0	34.0	41.0
21	38.320592182156616	40.0	37.0	41.0	34.0	41.0
22	38.33753875410678	40.0	37.0	41.0	34.0	41.0
23	38.28848878015432	40.0	37.0	41.0	34.0	41.0
24	38.22697927690845	40.0	37.0	41.0	34.0	41.0
25	38.102834778013786	40.0	37.0	41.0	34.0	41.0
26	38.02982554867754	40.0	37.0	41.0	34.0	41.0
27	37.979003745172996	40.0	37.0	41.0	34.0	41.0
28	37.9317868012879	40.0	37.0	41.0	34.0	41.0
29	37.89515456115499	40.0	36.0	41.0	34.0	41.0
30	37.575290814399736	40.0	36.0	41.0	33.0	41.0
31	37.605082031080904	40.0	36.0	41.0	33.0	41.0
32	37.55975036160548	40.0	36.0	41.0	33.0	41.0
33	37.48290789023911	40.0	36.0	41.0	33.0	41.0
34	37.457418621973375	40.0	36.0	41.0	33.0	41.0
35	37.4121003867574	40.0	36.0	41.0	33.0	41.0
36	37.31170915126827	40.0	36.0	41.0	32.0	41.0
37	37.24860395653868	39.0	35.0	41.0	32.0	41.0
38	37.17518274324593	39.0	35.0	41.0	32.0	41.0
39	37.10465138843072	39.0	35.0	41.0	32.0	41.0
40	36.99599061990151	39.0	35.0	41.0	31.0	41.0
41	36.91590750661637	39.0	35.0	41.0	31.0	41.0
42	36.90617811738259	39.0	35.0	41.0	31.0	41.0
43	36.834221238370034	39.0	35.0	41.0	31.0	41.0
44	36.6626612670895	39.0	35.0	41.0	31.0	41.0
45	36.595545199565926	39.0	35.0	40.0	31.0	41.0
46	36.41769500947115	39.0	35.0	40.0	31.0	41.0
47	36.25313130197379	39.0	35.0	40.0	30.0	41.0
48	36.16052298079349	38.0	35.0	40.0	30.0	41.0
49	36.06255886817857	38.0	35.0	40.0	30.0	41.0
50	35.913238567072035	38.0	35.0	40.0	30.0	41.0
51	34.77923481443556	36.0	34.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	9.0
11	10.0
12	5.0
13	4.0
14	10.0
15	16.0
16	28.0
17	41.0
18	103.0
19	197.0
20	355.0
21	564.0
22	960.0
23	1429.0
24	2130.0
25	3093.0
26	4091.0
27	5012.0
28	5969.0
29	7502.0
30	9843.0
31	13406.0
32	18729.0
33	29055.0
34	42134.0
35	54886.0
36	70261.0
37	116341.0
38	149415.0
39	134235.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.953390583181204	4.395092614291962	55.84994827810112	14.801568524425724
2	20.954310083605876	3.9532547478911946	52.08462389297971	23.007811275523228
3	21.291808534934297	4.403451709061707	51.87863191472529	22.426107841278704
4	19.35832006078256	4.610190035063417	50.87972008974086	25.15176981441317
5	17.501406865503657	7.946364465488134	50.44609204856037	24.10613662044784
6	15.507314954271273	31.384519852103733	39.53165186161519	13.57651333200981
7	80.14088060078008	1.8306417545739921	11.724227492764157	6.304250151881767
8	80.70108921990241	2.119627602328008	10.034048384231761	7.145234793537823
9	75.22065771148883	4.540182616366809	13.345593338995625	6.893566333148737
10	36.494464338758284	25.980812892112446	21.528997849025792	15.995724920103473
11	27.37155728442865	25.256258499034974	26.401006673841554	20.97117754269482
12	23.528762003137647	22.50701193708587	33.59460480140433	20.369621258372156
13	23.742217458865046	24.470652860228473	32.907964873889625	18.879164807016863
14	19.792545180160882	27.107499451434407	32.401344023023334	20.698611345381376
15	17.399903571871047	26.312042022363563	34.73024753369387	21.55780687207152
16	19.420864002006184	26.981963760338783	29.73538986967276	23.86178236798228
17	19.653724499163346	26.748655454533242	28.46421038647379	25.133409659829624
18	21.28046404917536	25.09967474165173	31.451691149360606	22.168170059812308
19	21.599900885019157	27.47529962130315	28.401367906151247	22.523431587526442
20	22.51149002356966	28.45570202215459	29.46819737613986	19.56461057813589
21	22.71001852435109	27.888776273306576	30.32933340697298	19.07187179536936
22	20.772649041913397	26.380108936917196	28.330166331058965	24.517075690110445
23	20.26707307789333	27.043462814716186	28.12447289190347	24.564991215487016
24	21.642442706615178	26.707457058882362	27.966844247674004	23.683255986828456
25	20.943413406495313	27.781451467245038	25.360747183656773	25.914387942602872
26	21.53019200542147	27.960873465695617	26.659840072604705	23.849094456278202
27	20.41410358411115	28.592880738108068	27.25512703585007	23.737888641930713
28	18.96544260660458	26.975246630613093	27.607253903025548	26.45205685975678
29	22.764203370804967	26.31816207389141	26.634016440548177	24.283618114755445
30	22.98094275662048	28.95441158689951	25.96484105032026	22.099804606159758
31	21.651100340483843	30.321272851302155	25.529571044095718	22.498055764118288
32	22.89481422658222	30.680116848203316	23.199025568381128	23.226043356833337
33	20.905349671383085	29.70105787329702	24.617235557797915	24.776356897521975
34	19.00753661955222	26.233675508897214	29.31952490487798	25.439262966672587
35	21.760962728886195	28.51720107653199	24.963540912544463	24.758295282037352
36	22.51178856266858	29.971534296918033	24.39153999901482	23.125137141398568
37	19.148894882890573	30.02497279562461	26.99315897654826	23.832973344936555
38	20.141238847698787	30.545027905942273	25.169831429897794	24.143901816461145
39	21.500636634628446	27.449177450147705	27.015847948066142	24.034337967157715
40	22.569257339210573	26.921957401455977	28.089842356428818	22.418942902904636
41	18.856326565949526	28.14731113297081	25.792733259793206	27.203629041286465
42	20.79727851757425	26.845829931231517	25.616147382782355	26.74074416841188
43	21.94008618823786	25.341640681325934	26.664467428637963	26.05380570179825
44	21.38540054244554	25.195505792404866	25.689737270665997	27.729356394483595
45	22.848689935799165	24.73247164998082	24.68380977685695	27.73502863736306
46	21.73110881899425	26.352494070267145	28.115516718935886	23.800880391802714
47	17.97220302449961	27.605910477080407	30.269327048090172	24.15255945032981
48	18.312985405916148	26.928674531181663	28.786931152405703	25.971408910496486
49	21.872616351882066	23.847303221684683	30.21155973244926	24.068520693983988
50	20.897587654811183	24.055384973631533	27.919077991846898	27.127949379710387
51	18.505095316070808	24.236598206675634	26.407126725369405	30.851179751884157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	98.0
1	256.0
2	414.0
3	1389.0
4	2364.0
5	1631.5
6	899.0
7	975.0
8	1051.0
9	1325.5
10	1600.0
11	1817.5
12	2035.0
13	2141.5
14	2248.0
15	2273.5
16	2299.0
17	2336.5
18	2374.0
19	2395.0
20	2416.0
21	2480.5
22	2545.0
23	2946.5
24	3348.0
25	3457.5
26	4207.0
27	4847.0
28	5650.5
29	6454.0
30	7658.0
31	8862.0
32	10820.0
33	12778.0
34	14658.5
35	16539.0
36	17550.5
37	18562.0
38	20781.0
39	23000.0
40	26600.0
41	30200.0
42	34830.5
43	39461.0
44	45744.0
45	52027.0
46	62687.5
47	73348.0
48	85005.5
49	96663.0
50	88023.5
51	79384.0
52	64932.5
53	50481.0
54	43502.0
55	36523.0
56	32114.0
57	27705.0
58	24167.0
59	20629.0
60	17711.5
61	14794.0
62	12760.5
63	10727.0
64	9065.5
65	7404.0
66	6133.5
67	4863.0
68	3975.5
69	3088.0
70	2434.0
71	1780.0
72	1462.5
73	1145.0
74	938.5
75	534.0
76	336.0
77	255.5
78	175.0
79	131.0
80	87.0
81	67.0
82	47.0
83	33.5
84	20.0
85	12.0
86	4.0
87	3.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.79406225774259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13613656523104	31.725864437210046
2	10.701407018969483	9.159133564304472
3	3.9822215428048193	5.112463098807397
4	2.2416373262326488	3.8371506919231844
5	1.6057299255342126	3.4357853201215733
6	1.248079164569779	3.204622648271429
7	0.9747281551239825	2.91988041483252
8	0.7678708317137056	2.6288249742608722
9	0.616646432089796	2.3749925225279007
>10	3.577531192007915	23.924673178757512
>50	0.09114029989381761	2.686033569245518
>100	0.049944883195841165	4.037084824683629
>500	0.0025519283384736295	0.7800527156709864
>1k	0.004010173103315704	3.346270139685398
>5k	3.645611912105185E-4	0.827167899697514
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	5302	0.7914271512354295	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	4280	0.6388736716875967	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	4156	0.6203642475545915	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	2759	0.41183468695936437	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	1526	0.22778533247553098	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1412	0.21076860383712304	No Hit
TCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1391	0.20763394329846893	TruSeq Adapter, Index 23 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	1273	0.1900201364622221	No Hit
GCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1227	0.18315373718707503	TruSeq Adapter, Index 23 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	1191	0.17778003340652518	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.16957020818624063	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	1098	0.1638979653067713	No Hit
TTCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	923	0.13777579415132052	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	819	0.12225176100750974	No Hit
TTCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	778	0.11613170947966128	No Hit
GCCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	728	0.10866823200667533	No Hit
TGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	725	0.10822042335829618	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4926954945971888E-4	0.0	0.0	0.859643335338521	0.0
2	1.4926954945971888E-4	0.0	0.0	2.7307371378160967	0.0
3	1.4926954945971888E-4	0.0	0.0	3.7672648892643847	0.0
4	1.4926954945971888E-4	0.0	0.0	4.886935779761736	0.0
5	1.4926954945971888E-4	0.0	0.0	6.594280886482	0.0
6	1.4926954945971888E-4	0.0	0.0	7.571100818146401	0.0
7	1.4926954945971888E-4	0.0	0.0	8.794215506419336	0.0
8	1.4926954945971888E-4	0.0	0.0	10.600227785332475	0.0
9	1.4926954945971888E-4	0.0	0.0	11.419120533668494	0.0
10	1.4926954945971888E-4	0.0	0.0	12.922115627178403	0.0
11	1.4926954945971888E-4	0.0	0.0	15.745847694307905	0.0
12	1.4926954945971888E-4	0.0	0.0	17.9459315837947	0.0
13	2.9853909891943775E-4	0.0	0.0	18.790797233736708	0.0
14	2.9853909891943775E-4	0.0	0.0	19.07082690852314	0.0
15	2.9853909891943775E-4	0.0	0.0	19.485199177823322	0.0
16	2.9853909891943775E-4	0.0	0.0	20.482767576862624	0.0
17	2.9853909891943775E-4	0.0	0.0	21.98695682676821	0.0
18	2.9853909891943775E-4	0.0	0.0	23.58548443193234	0.0
19	2.9853909891943775E-4	0.0	0.0	24.570663458366482	0.0
20	2.9853909891943775E-4	0.0	0.0	25.577934378120666	0.0
21	2.9853909891943775E-4	0.0	0.0	26.833291289076904	0.0
22	2.9853909891943775E-4	0.0	0.0	28.079393487966634	0.0
23	2.9853909891943775E-4	0.0	0.0	29.339527024505582	0.0
24	2.9853909891943775E-4	0.0	0.0	30.241861450989582	0.0
25	2.9853909891943775E-4	0.0	0.0	31.053141452303155	0.0
26	2.9853909891943775E-4	0.0	0.0	31.759037151698166	0.0
27	2.9853909891943775E-4	0.0	0.0	32.502548777557024	0.0
28	2.9853909891943775E-4	0.0	0.0	33.235611534953705	0.0
29	2.9853909891943775E-4	0.0	0.0	34.003752636473415	0.0
30	2.9853909891943775E-4	0.0	0.0	34.79398563131317	0.0
31	2.9853909891943775E-4	0.0	0.0	35.53257136203986	0.0
32	2.9853909891943775E-4	0.0	0.0	36.224584993335114	0.0
33	2.9853909891943775E-4	0.0	0.0	36.902865826080074	0.0
34	4.478086483791566E-4	0.0	0.0	37.598760465661286	0.0
35	4.478086483791566E-4	0.0	0.0	38.29987953947359	0.0
36	4.478086483791566E-4	0.0	0.0	38.931289733688196	0.0
37	4.478086483791566E-4	0.0	0.0	39.55284813763846	0.0
38	4.478086483791566E-4	0.0	0.0	40.21799325003097	0.0
39	4.478086483791566E-4	0.0	0.0	41.01763022648669	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	20	7.031176E-4	45.000004	27
CGTCTCG	20	7.031176E-4	45.000004	42
GCGTTAG	30	2.1641408E-6	45.000004	1
CCTCGAC	20	7.031176E-4	45.000004	26
TCGGATC	20	7.031176E-4	45.000004	38
CCTCCGA	20	7.031176E-4	45.000004	30
AAACCGC	20	7.031176E-4	45.000004	26
CGTGTGA	20	7.031176E-4	45.000004	18
CCGTTAG	30	2.1641408E-6	45.000004	1
TCGTTGA	35	1.2109194E-7	45.0	24
TCGTTAG	90	0.0	45.0	1
CGTTGAT	35	1.2109194E-7	45.0	25
CGTTAGG	150	0.0	45.0	2
CGTAAGG	25	3.8890576E-5	45.0	2
TAGGGCG	405	0.0	43.88889	5
AGGGCGA	565	0.0	43.805305	6
AGGGCGC	90	0.0	42.5	6
TTAGGGA	1255	0.0	42.131474	4
CTAGGGA	850	0.0	42.088238	4
CGTTTTT	770	0.0	42.07792	1
>>END_MODULE