FastQCFastQC Report
Sat 14 Jan 2017
SRR2934283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719047
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC178601.0389477425573588No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG135260.7868313082772024No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC119790.6968395861195185No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT59630.3468782412580924TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC58290.3390832246006072TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC48110.27986436671015974TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC47330.27532696895431014TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.264797879290095No Hit
GAATCAGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC41250.23995853516512347No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC39810.23158180084663188No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT37430.21773692051468052No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT29600.17218842765788253No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA29470.17143219469857426No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT28210.16410255216989414No Hit
GCTGTATCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC21700.1262327324383801TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG18020.10482552251334606TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC17750.10325488482862888TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG17580.10226596480491806TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC207.0342625E-445.00000419
CGCGCAT302.1661326E-645.00000436
CGTCTAT351.2123928E-745.0000041
CTTACGT207.0342625E-445.00000422
CGTTTAT9550.042.879581
AGGGCGA10050.042.537316
TTAGGGA39300.042.137414
CTTAGGG33850.041.8759233
TAGGGCG7550.041.7218555
TAGGGTA9500.041.6842085
CGTTTTT24500.041.5102041
CGTTAGG4500.041.52
CTAGGGT8000.041.0625044
TCTTAGG20050.040.847882
CTAGGGA22250.040.6516844
TTTAGGG64450.040.5314183
TCGTTAG3000.040.5000041
CGAGGGT2000.040.5000044
TAGGGAC18600.040.403225
GTTAGGG23400.040.288463
Produced by FastQC (version 0.11.5)