##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934283.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1719047
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.197569932642914	33.0	31.0	34.0	30.0	34.0
2	32.3430202897303	34.0	31.0	34.0	30.0	34.0
3	32.409864884438875	34.0	31.0	34.0	30.0	34.0
4	35.94197249987929	37.0	35.0	37.0	35.0	37.0
5	35.03454995704015	37.0	35.0	37.0	35.0	37.0
6	25.67101888430043	35.0	0.0	37.0	0.0	37.0
7	30.867450395480752	36.0	19.0	37.0	17.0	37.0
8	34.52758359719077	37.0	32.0	37.0	32.0	37.0
9	37.13295273485833	39.0	37.0	39.0	34.0	39.0
10	37.15877052808911	39.0	37.0	39.0	34.0	39.0
11	37.2700281027802	39.0	37.0	39.0	34.0	39.0
12	37.145402656239185	39.0	37.0	39.0	34.0	39.0
13	37.19273004170334	39.0	37.0	39.0	34.0	39.0
14	38.388879419818075	40.0	38.0	41.0	34.0	41.0
15	38.4425609072934	40.0	38.0	41.0	34.0	41.0
16	38.538874736990905	40.0	38.0	41.0	34.0	41.0
17	38.48570632449258	40.0	38.0	41.0	34.0	41.0
18	38.46553991833847	40.0	38.0	41.0	34.0	41.0
19	38.48158776345266	40.0	38.0	41.0	34.0	41.0
20	38.402897652013	40.0	38.0	41.0	34.0	41.0
21	38.34304995733101	40.0	37.0	41.0	34.0	41.0
22	38.349959017990784	40.0	37.0	41.0	34.0	41.0
23	38.27209378219444	40.0	37.0	41.0	34.0	41.0
24	38.20958240234269	40.0	37.0	41.0	34.0	41.0
25	38.10571206022872	40.0	37.0	41.0	34.0	41.0
26	38.07975814506526	40.0	37.0	41.0	34.0	41.0
27	38.06911562045715	40.0	37.0	41.0	34.0	41.0
28	38.03039765637589	40.0	37.0	41.0	34.0	41.0
29	38.03740386388505	40.0	37.0	41.0	34.0	41.0
30	37.79378050745559	40.0	37.0	41.0	33.0	41.0
31	37.84373085785322	40.0	37.0	41.0	33.0	41.0
32	37.7621699697565	40.0	37.0	41.0	33.0	41.0
33	37.670890324697346	40.0	37.0	41.0	33.0	41.0
34	37.62594332790203	40.0	37.0	41.0	33.0	41.0
35	37.626685599637476	40.0	37.0	41.0	33.0	41.0
36	37.548596984259305	40.0	37.0	41.0	33.0	41.0
37	37.51042990680301	40.0	37.0	41.0	33.0	41.0
38	37.39557615353158	40.0	36.0	41.0	33.0	41.0
39	37.325279064504926	40.0	36.0	41.0	33.0	41.0
40	37.241101610369	40.0	36.0	41.0	32.0	41.0
41	37.20296652738407	40.0	36.0	41.0	32.0	41.0
42	37.1945833941713	39.0	36.0	41.0	32.0	41.0
43	37.10970264338322	39.0	36.0	41.0	32.0	41.0
44	36.98349725167491	39.0	35.0	41.0	31.0	41.0
45	36.9812343699736	39.0	35.0	41.0	31.0	41.0
46	36.90643827655672	39.0	35.0	41.0	31.0	41.0
47	36.81986530909277	39.0	35.0	41.0	31.0	41.0
48	36.81521913013431	39.0	35.0	41.0	31.0	41.0
49	36.80641890535861	39.0	35.0	41.0	31.0	41.0
50	36.710636183885605	39.0	35.0	40.0	31.0	41.0
51	35.666792705493215	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	8.0
11	13.0
12	5.0
13	10.0
14	15.0
15	23.0
16	49.0
17	108.0
18	212.0
19	449.0
20	857.0
21	1483.0
22	2298.0
23	3623.0
24	5475.0
25	8338.0
26	10881.0
27	12872.0
28	14485.0
29	17531.0
30	23016.0
31	31264.0
32	43658.0
33	66215.0
34	98106.0
35	132258.0
36	171053.0
37	284756.0
38	391691.0
39	398093.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.735541843823935	4.250087403078566	54.60036869265355	13.414002060443956
2	19.97874403666683	8.770499003226789	50.37820373730328	20.8725532228031
3	19.68538382022132	8.698715043858602	50.1141620909725	21.50173904494758
4	17.694687812491456	4.178245271944281	54.16786161169532	23.95920530386895
5	19.40546128174506	7.849698117619821	49.068757282377966	23.676083318257152
6	14.67935431666499	34.10284884590125	38.763861604714705	12.453935232719058
7	79.99228642381505	1.6875047628133495	13.52592453842158	4.794284274950016
8	80.4812201178909	4.9496610622048145	9.008945072473294	5.56017374743099
9	75.61579177299981	4.249563857183661	10.989868223498252	9.14477614631828
10	37.11992749471073	26.047222676285177	20.21236184932698	16.62048797967711
11	26.941497236550248	24.885997881384277	26.76826171710256	21.404243164962914
12	23.17121056026973	21.250087984796227	32.357288660519465	23.22141279441458
13	22.930030418016496	22.799143944290062	35.23120659295528	19.03961904473816
14	20.54719853500224	26.14547478922915	31.114507049545477	22.192819626223134
15	17.20656852314102	25.775851387425707	36.0287996779611	20.98878041147217
16	19.037757548222938	24.97761841299278	31.077626149837673	24.90699788894661
17	19.15049443092597	25.473009173105797	30.923296454372682	24.453199941595546
18	19.746231487562586	24.40578995222353	32.13897002234378	23.709008537870112
19	20.54981626447677	27.02363577028435	29.85840410413444	22.568143861104435
20	22.307243490143087	26.564835051048636	31.861956072172546	19.265965386635735
21	21.856354131097056	28.69572501508103	29.91936811500791	19.528552738814007
22	20.452960273919214	24.259953334609232	29.965032951396907	25.322053440074644
23	20.0767634625464	27.199721706270974	28.507888382342077	24.21562644884055
24	21.16981094757735	24.38961820124755	29.369877612421302	25.0706932387538
25	18.653242174297734	28.838420357325887	27.295414261506522	25.212923206869853
26	18.385361191404307	25.575856855571722	30.773096954300843	25.26568499872313
27	19.551588758189858	26.740164754075952	29.56841785012277	24.139828637611423
28	17.024898097608734	24.247853607260303	34.20127547414352	24.525972820987445
29	19.66461649972339	22.175600783457348	32.042753921213325	26.11702879560594
30	19.680962765997673	25.933264186494025	30.754947363277445	23.630825684230857
31	20.130514174423386	25.901909604565788	29.10595230962272	24.86162391138811
32	20.840209720851146	27.802323031307463	28.440001931302632	22.917465316538756
33	20.285483759315483	25.594646336022226	26.58211206558052	27.537757839081774
34	19.39551390974185	23.768634598123263	30.86436845531274	25.971483036822146
35	18.843638364745118	22.993263127767886	30.732144030965998	27.430954476521002
36	19.559732805443947	24.967147495094665	30.846975097248652	24.626144602212737
37	19.12629497622811	23.994108363529328	31.57231884875748	25.307277811485086
38	20.536029555910922	25.889286331321948	30.055548219449495	23.519135893317635
39	23.08156786870865	24.678091989340604	28.56041748713095	23.679922654819794
40	19.463167673716892	25.010543632605742	30.69177282529215	24.834515868385214
41	18.577269847770307	23.857695572023335	29.83339024471117	27.731644335495194
42	20.97132888164198	21.86670870546297	32.586543590722066	24.575418822172985
43	23.305063794067294	22.23255094246987	29.846246205019412	24.61613905844343
44	20.486409039427077	22.409800313778504	29.29419614472437	27.80959450207004
45	20.399907623235432	21.678930244490115	28.692409224413296	29.228752907861157
46	22.56744579991123	23.008678645784553	29.37691639611948	25.04695915818474
47	17.478521529661492	22.610958280954506	34.873857433799074	25.036662755584928
48	19.215704980724784	22.75283921847396	31.733803671452847	26.297652129348414
49	21.203899602512323	20.633176405298983	34.137461046731126	24.02546294545757
50	21.50447311795431	20.286181820508688	30.39009404629425	27.819251015242745
51	18.868361365337886	20.241913106506104	28.466295569580126	32.42342995857588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	243.0
1	809.5
2	1376.0
3	5809.0
4	10242.0
5	6861.0
6	3480.0
7	3447.0
8	3414.0
9	3500.0
10	3586.0
11	3720.0
12	3854.0
13	4007.0
14	4160.0
15	4186.5
16	4213.0
17	3999.5
18	3786.0
19	4019.0
20	4252.0
21	4638.0
22	5024.0
23	5717.0
24	6410.0
25	7145.0
26	10012.5
27	12145.0
28	14847.0
29	17549.0
30	20281.0
31	23013.0
32	26108.5
33	29204.0
34	35117.0
35	41030.0
36	44816.0
37	48602.0
38	55499.0
39	62396.0
40	72262.0
41	82128.0
42	94098.0
43	106068.0
44	124037.0
45	142006.0
46	193523.5
47	245041.0
48	236702.5
49	228364.0
50	213135.5
51	197907.0
52	164369.0
53	130831.0
54	106774.5
55	82718.0
56	69891.5
57	57065.0
58	51558.5
59	46052.0
60	40701.5
61	35351.0
62	29821.5
63	24292.0
64	20012.5
65	15733.0
66	13140.5
67	10548.0
68	8964.0
69	7380.0
70	6180.5
71	4981.0
72	4090.0
73	3199.0
74	2477.0
75	1378.0
76	1001.0
77	741.5
78	482.0
79	317.0
80	152.0
81	120.0
82	88.0
83	58.5
84	29.0
85	19.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1719047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.42075608848047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.1117906461895	26.23636216697664
2	13.427654071216988	10.049459356790054
3	5.1321447607369635	5.761462119069327
4	2.660353257551453	3.982097214401095
5	1.5200744505898336	2.8441167625926562
6	1.0500313584730119	2.3575780410404628
7	0.7272786351015826	1.905072148874952
8	0.5591983649430676	1.6740500495689297
9	0.48120698545105867	1.6206416307573333
>10	4.156604832786576	29.289119371920087
>50	0.11055963110084188	2.8141101253956577
>100	0.05536895712079518	3.7232527255372525
>500	0.0036912505342461812	0.9323075789505728
>1k	0.00316392902935387	3.1823694994205316
>5k	3.515476699282077E-4	0.7756293897971748
>10k+	5.273215048923116E-4	2.852371818907266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	17860	1.0389477425573588	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	13526	0.7868313082772024	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	11979	0.6968395861195185	No Hit
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	5963	0.3468782412580924	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	5829	0.3390832246006072	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	4811	0.27986436671015974	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	4733	0.27532696895431014	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4552	0.264797879290095	No Hit
GAATCAGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	4125	0.23995853516512347	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTC	3981	0.23158180084663188	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCT	3743	0.21773692051468052	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCT	2960	0.17218842765788253	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTGACTGTCGTA	2947	0.17143219469857426	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTGACTGT	2821	0.16410255216989414	No Hit
GCTGTATCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	2170	0.1262327324383801	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	1802	0.10482552251334606	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	1775	0.10325488482862888	TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTG	1758	0.10226596480491806	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1634353220127198E-4	0.0	0.0	0.36339902283067305	0.0
2	1.1634353220127198E-4	0.0	0.0	1.3324243025350675	0.0
3	1.1634353220127198E-4	0.0	0.0	1.8685352989185287	0.0
4	1.1634353220127198E-4	0.0	0.0	2.6810785278122125	0.0
5	1.1634353220127198E-4	0.0	0.0	4.421635941309342	0.0
6	1.1634353220127198E-4	0.0	0.0	5.2049769436205064	0.0
7	1.1634353220127198E-4	0.0	0.0	6.334207267166052	0.0
8	1.7451529830190797E-4	0.0	0.0	8.046609545870474	0.0
9	1.7451529830190797E-4	0.0	0.0	8.669803676106588	0.0
10	2.3268706440254396E-4	0.0	0.0	10.879341867907044	0.0
11	2.3268706440254396E-4	0.0	0.0	13.612309611081024	0.0
12	2.3268706440254396E-4	0.0	0.0	17.184405080256678	0.0
13	2.9085883050317997E-4	0.0	0.0	18.077050831070938	0.0
14	3.4903059660381595E-4	0.0	0.0	18.391411055078773	0.0
15	3.4903059660381595E-4	0.0	0.0	18.949103776685572	0.0
16	3.4903059660381595E-4	0.0	0.0	19.929065348416884	0.0
17	3.4903059660381595E-4	0.0	0.0	21.322104631228814	0.0
18	3.4903059660381595E-4	0.0	0.0	22.717761643515274	0.0
19	3.4903059660381595E-4	0.0	0.0	24.046521124786	0.0
20	3.4903059660381595E-4	0.0	0.0	25.162197426830097	0.0
21	3.4903059660381595E-4	0.0	0.0	26.812995805233946	0.0
22	3.4903059660381595E-4	0.0	0.0	28.592877332615107	0.0
23	3.4903059660381595E-4	0.0	0.0	30.306152187811037	0.0
24	3.4903059660381595E-4	0.0	0.0	31.532354845446342	0.0
25	3.4903059660381595E-4	0.0	0.0	32.62970704116874	0.0
26	3.4903059660381595E-4	0.0	0.0	33.55731402340948	0.0
27	3.4903059660381595E-4	0.0	0.0	34.48486283388412	0.0
28	3.4903059660381595E-4	0.0	0.0	35.44114849681248	0.0
29	3.4903059660381595E-4	0.0	0.0	36.42820702400807	0.0
30	3.4903059660381595E-4	0.0	0.0	37.49542624489034	0.0
31	3.4903059660381595E-4	0.0	0.0	38.49452632766876	0.0
32	3.4903059660381595E-4	0.0	0.0	39.50770397784354	0.0
33	3.4903059660381595E-4	0.0	0.0	40.44008104490453	0.0
34	3.4903059660381595E-4	0.0	0.0	41.32371017197319	0.0
35	3.4903059660381595E-4	0.0	0.0	42.27987949136934	0.0
36	3.4903059660381595E-4	0.0	0.0	43.17473576929543	0.0
37	3.4903059660381595E-4	0.0	0.0	44.07127902843843	0.0
38	3.4903059660381595E-4	0.0	0.0	44.93565330092778	0.0
39	3.4903059660381595E-4	0.0	0.0	45.81462868670839	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAC	20	7.0342625E-4	45.000004	19
CGCGCAT	30	2.1661326E-6	45.000004	36
CGTCTAT	35	1.2123928E-7	45.000004	1
CTTACGT	20	7.0342625E-4	45.000004	22
CGTTTAT	955	0.0	42.87958	1
AGGGCGA	1005	0.0	42.53731	6
TTAGGGA	3930	0.0	42.13741	4
CTTAGGG	3385	0.0	41.875923	3
TAGGGCG	755	0.0	41.721855	5
TAGGGTA	950	0.0	41.684208	5
CGTTTTT	2450	0.0	41.510204	1
CGTTAGG	450	0.0	41.5	2
CTAGGGT	800	0.0	41.062504	4
TCTTAGG	2005	0.0	40.84788	2
CTAGGGA	2225	0.0	40.651684	4
TTTAGGG	6445	0.0	40.531418	3
TCGTTAG	300	0.0	40.500004	1
CGAGGGT	200	0.0	40.500004	4
TAGGGAC	1860	0.0	40.40322	5
GTTAGGG	2340	0.0	40.28846	3
>>END_MODULE