FastQCFastQC Report
Sat 14 Jan 2017
SRR2934282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511852
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC85580.5660606990631358No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77750.5142699153091704No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG74780.4946251352645629No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC61620.40757957789519084RNA PCR Primer, Index 29 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT39450.26093823998645366RNA PCR Primer, Index 29 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC36150.2391107066035564Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC35120.23229787042647032RNA PCR Primer, Index 29 (96% over 25bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.20147474752819722No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC29590.19572021599997885Illumina PCR Primer Index 8 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC22670.14994853993644883RNA PCR Primer, Index 29 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC20440.13519841889285458No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT20430.13513227485230037No Hit
CGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG19630.12984075160796163RNA PCR Primer, Index 29 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA207.0339936E-445.040
CGTTTAT14150.043.0918731
TCGTTAG2000.042.751
CGTTTTT38600.042.4935231
CGTTAGG4450.042.4719122
CCGTTAG700.041.7857171
TTAGGGA28000.041.6254
GTAGGGA7650.041.470594
TTTAGGG50800.041.323823
GCGTTAG1700.041.029411
CGTAGGG6200.040.6451643
TAGGGAA12250.040.591845
TCTCGCG1000.040.51
CTTAGGG27600.040.434783
TCTCGAG1450.040.3448261
TTAGGGG50100.040.329344
TAGGGTA6200.040.282265
TAGGGAT15650.040.2555925
AGGGCGA7500.040.1999976
GTTAGGG22850.040.0765843
Produced by FastQC (version 0.11.5)