##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934282.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1511852
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2384353759495	33.0	31.0	34.0	30.0	34.0
2	32.40261480621119	34.0	31.0	34.0	30.0	34.0
3	32.437449565169075	34.0	31.0	34.0	30.0	34.0
4	35.97514439244053	37.0	35.0	37.0	35.0	37.0
5	35.04925482123912	37.0	35.0	37.0	35.0	37.0
6	25.68221095715718	35.0	0.0	37.0	0.0	37.0
7	30.87061167362943	37.0	19.0	37.0	17.0	37.0
8	34.59266846225689	37.0	32.0	37.0	32.0	37.0
9	37.17163386363215	39.0	37.0	39.0	34.0	39.0
10	37.23422200056619	39.0	37.0	39.0	34.0	39.0
11	37.307994433317546	39.0	37.0	39.0	34.0	39.0
12	37.08118188817424	39.0	37.0	39.0	34.0	39.0
13	37.07454234938341	39.0	37.0	39.0	33.0	39.0
14	38.09702140156576	40.0	37.0	41.0	33.0	41.0
15	38.23691803165919	40.0	38.0	41.0	33.0	41.0
16	38.37757664109979	40.0	38.0	41.0	34.0	41.0
17	38.36482274720012	40.0	37.0	41.0	34.0	41.0
18	38.3378961697309	40.0	37.0	41.0	34.0	41.0
19	38.30784230202427	40.0	37.0	41.0	34.0	41.0
20	38.187449565169075	40.0	37.0	41.0	34.0	41.0
21	38.106534898918675	40.0	37.0	41.0	34.0	41.0
22	38.12856351018486	40.0	37.0	41.0	34.0	41.0
23	38.07268105608221	40.0	36.0	41.0	34.0	41.0
24	38.00091080343843	40.0	36.0	41.0	34.0	41.0
25	37.88825493500686	40.0	36.0	41.0	34.0	41.0
26	37.866375809272334	40.0	36.0	41.0	34.0	41.0
27	37.82762003159039	40.0	36.0	41.0	34.0	41.0
28	37.768164476417006	40.0	36.0	41.0	33.0	41.0
29	37.73741874204618	40.0	36.0	41.0	33.0	41.0
30	37.444480015239584	40.0	36.0	41.0	33.0	41.0
31	37.446350568706464	40.0	35.0	41.0	33.0	41.0
32	37.340287276796936	39.0	35.0	41.0	33.0	41.0
33	37.20837555527922	39.0	35.0	41.0	32.0	41.0
34	37.076793231083464	39.0	35.0	41.0	32.0	41.0
35	37.027524519595836	39.0	35.0	41.0	32.0	41.0
36	36.945173204784595	39.0	35.0	41.0	31.0	41.0
37	36.87183798414131	39.0	35.0	41.0	31.0	41.0
38	36.81334284043676	39.0	35.0	41.0	31.0	41.0
39	36.735283612417085	39.0	35.0	41.0	31.0	41.0
40	36.60625709394835	39.0	35.0	41.0	31.0	41.0
41	36.497792773366704	39.0	35.0	41.0	30.0	41.0
42	36.52821770914084	39.0	35.0	41.0	31.0	41.0
43	36.49712008847427	39.0	35.0	41.0	31.0	41.0
44	36.39945444395351	39.0	35.0	41.0	30.0	41.0
45	36.36363017014893	39.0	35.0	40.0	30.0	41.0
46	36.27083140413215	39.0	35.0	40.0	30.0	41.0
47	36.16580458933811	39.0	35.0	40.0	30.0	41.0
48	36.14751047060162	39.0	35.0	40.0	30.0	41.0
49	36.13201887486341	39.0	35.0	40.0	30.0	41.0
50	36.041235517762324	38.0	35.0	40.0	30.0	41.0
51	35.10212772149655	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	14.0
11	12.0
12	9.0
13	11.0
14	10.0
15	36.0
16	80.0
17	140.0
18	279.0
19	579.0
20	1007.0
21	1646.0
22	2677.0
23	4300.0
24	6844.0
25	10187.0
26	13464.0
27	15192.0
28	16007.0
29	18920.0
30	23255.0
31	30963.0
32	43486.0
33	68349.0
34	98980.0
35	119920.0
36	149182.0
37	244043.0
38	324223.0
39	317845.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.890364929900546	4.166479258551763	53.82458071292693	16.118575098620763
2	22.102163439278446	6.867669586705577	49.51926511325183	21.510901860764147
3	20.452994076139728	6.780425597214543	51.05261626138008	21.71396406526565
4	18.046938456938907	4.402878059492596	53.153681709585335	24.396501773983168
5	18.940478300786058	7.94919079380786	49.80262618298617	23.30770472241992
6	15.07574815524271	33.0348473263256	39.46881043911706	12.420594079314643
7	80.24654529676185	1.9179125999105735	13.15366848077722	4.681873622550356
8	81.29248101004596	3.7140540211608015	10.082666821884683	4.91079814690856
9	76.48685188761863	4.044906512013081	12.060307490415727	7.407934109952562
10	40.19434441995645	22.894172180874847	21.12336392715689	15.788119472011811
11	28.922540036987748	23.03102420078156	27.134203612522917	20.912232149707776
12	25.095181274357543	20.346766747009628	32.81835788159159	21.739694097041244
13	24.193108849278897	21.479748017663105	35.28718419527838	19.039958937779623
14	20.764929371393496	23.61626667160542	34.79923960810979	20.81956434889129
15	18.093570005529642	23.150811058225276	37.524374078944234	21.231244857300847
16	21.100015080841246	23.508253453380355	32.03931337194382	23.352418093834583
17	21.90492191034572	24.43493146154518	30.451724110561084	23.208422517548012
18	22.528726356812705	23.28455430822594	32.22630257459064	21.960416760370723
19	22.216328053275056	26.243507962419603	29.43667766421581	22.103486320089534
20	22.261636721054707	27.12877980119747	31.2908274090321	19.31875606871572
21	22.496580353103347	27.485494611906457	31.2439974283197	18.773927606670494
22	21.155311498744584	22.823530345562926	32.27915166299346	23.742006492699023
23	20.324542349383407	25.683797091249673	30.752547207001747	23.23911335236518
24	21.181901403047387	24.627146043395783	30.45384071985882	23.73711183369801
25	20.75844725541918	27.350626913216374	27.489992406664143	24.400933424700302
26	19.592460108529142	25.557660405912745	29.24175117670248	25.608128308855626
27	20.04607593865008	25.535171432124308	30.430028865259295	23.988723763966313
28	17.54880768752497	24.646195527075403	31.234604974561	26.57039181083863
29	19.24407944692999	24.02192807232454	29.962192066419203	26.77180041432627
30	20.854223826141713	26.55683228252501	29.455197995570998	23.133745895762285
31	20.774586401314416	26.36031833803838	27.820381889232543	25.044713371414662
32	21.014556980445175	26.698049809108298	27.07877490653847	25.208618303908054
33	20.59348401827692	26.46621494696571	26.44776075965108	26.49254027510629
34	18.398427888444107	26.106722086553447	28.71749351126962	26.777356513732826
35	17.308506388191436	27.12143781269595	27.19214579204843	28.377910007064184
36	19.49383934406278	28.16155285041128	24.613123506798285	27.731484298727654
37	17.770257935300545	29.0031034783828	27.929453412106476	25.297185174210174
38	18.60698004831161	27.030225180771662	27.78969105441538	26.57310371650135
39	19.206509631895187	26.25342956850274	28.79812309670523	25.741937702896845
40	20.618817185809192	26.689384939795698	28.054201072591763	24.63759680180335
41	17.923645965345813	26.51628598566526	26.14343202906105	29.41663601992788
42	18.427465122247416	23.7135645552607	28.61424266396446	29.24472765852742
43	19.182763921336214	23.703907525339783	28.872535142328747	28.24079341099526
44	19.966967666147216	23.542714498509113	28.36977429007601	28.120543545267658
45	19.525456195447703	23.67744990911809	27.049010088289066	29.748083807145143
46	20.529192010858203	25.132288081108467	27.682140844474194	26.656379063559132
47	16.548643650304395	25.28772657641092	31.420734304680614	26.742895468604072
48	17.584723901545917	26.19158489058453	29.28983789418541	26.933853313684143
49	19.53650225022026	23.442903141312772	30.80413955863405	26.21645504983292
50	18.81791339363906	23.65013242036919	28.997150514732922	28.534803671258828
51	17.407987025185005	23.841222553530372	26.566886176689252	32.18390424459537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	163.0
1	750.5
2	1338.0
3	9097.0
4	16856.0
5	10812.5
6	4769.0
7	4655.5
8	4542.0
9	4749.0
10	4956.0
11	4972.0
12	4988.0
13	4982.0
14	4976.0
15	4817.5
16	4659.0
17	4608.0
18	4557.0
19	4526.0
20	4495.0
21	4833.0
22	5171.0
23	5673.0
24	6175.0
25	6954.5
26	9350.5
27	10967.0
28	13368.0
29	15769.0
30	17646.0
31	19523.0
32	23558.5
33	27594.0
34	31636.0
35	35678.0
36	39146.0
37	42614.0
38	46575.5
39	50537.0
40	57538.5
41	64540.0
42	73879.5
43	83219.0
44	95470.0
45	107721.0
46	140058.0
47	172395.0
48	175372.5
49	178350.0
50	169865.0
51	161380.0
52	138527.0
53	115674.0
54	101180.0
55	86686.0
56	77950.5
57	69215.0
58	62913.5
59	56612.0
60	49688.5
61	42765.0
62	37033.0
63	31301.0
64	26620.0
65	21939.0
66	17993.0
67	14047.0
68	11819.5
69	9592.0
70	8413.0
71	7234.0
72	6103.0
73	4972.0
74	3986.0
75	2254.0
76	1508.0
77	1232.5
78	957.0
79	661.0
80	365.0
81	259.0
82	153.0
83	125.0
84	97.0
85	66.0
86	35.0
87	29.0
88	23.0
89	14.0
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1511852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.83154109635538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7906711521085	28.654054793707466
2	11.37421328779656	8.833564614475671
3	4.107251592723943	4.7847272704779265
4	2.0624339115973003	3.203499487868302
5	1.2450348133645839	2.4173310260779997
6	0.8794705738285555	2.0490718638395284
7	0.7236657835811837	1.967074033061127
8	0.5602672432571287	1.7404832385184743
9	0.5044622691950869	1.7630142604028736
>10	4.590061906630651	32.54363511838703
>50	0.10298428307506016	2.752044827717073
>100	0.05268510930920995	3.6545802138051102
>500	0.002643695262546151	0.7348900750608233
>1k	0.003399036766130766	2.7041784262715067
>5k	7.553415035846146E-4	2.197850750329082
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	8558	0.5660606990631358	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7775	0.5142699153091704	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	7478	0.4946251352645629	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	6162	0.40757957789519084	RNA PCR Primer, Index 29 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	3945	0.26093823998645366	RNA PCR Primer, Index 29 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	3615	0.2391107066035564	Illumina PCR Primer Index 8 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	3512	0.23229787042647032	RNA PCR Primer, Index 29 (96% over 25bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3046	0.20147474752819722	No Hit
TCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	2959	0.19572021599997885	Illumina PCR Primer Index 8 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	2267	0.14994853993644883	RNA PCR Primer, Index 29 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	2044	0.13519841889285458	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	2043	0.13513227485230037	No Hit
CGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG	1963	0.12984075160796163	RNA PCR Primer, Index 29 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.614404055423415E-5	0.0	0.0	0.2776065382061207	0.0
2	6.614404055423415E-5	0.0	0.0	1.0065800091543353	0.0
3	6.614404055423415E-5	0.0	0.0	1.4697205811150826	0.0
4	6.614404055423415E-5	0.0	0.0	2.080494651592881	0.0
5	6.614404055423415E-5	0.0	0.0	3.427650325560968	0.0
6	6.614404055423415E-5	0.0	0.0	4.298965771781894	0.0
7	6.614404055423415E-5	0.0	0.0	5.424605053933851	0.0
8	6.614404055423415E-5	0.0	0.0	7.014575500776531	0.0
9	6.614404055423415E-5	0.0	0.0	7.732899781195514	0.0
10	1.322880811084683E-4	0.0	0.0	9.515613962213232	0.0
11	1.9843212166270244E-4	0.0	0.0	11.784156121101802	0.0
12	1.9843212166270244E-4	0.0	0.0	14.283937845767973	0.0
13	1.9843212166270244E-4	0.0	0.0	14.990819207171072	0.0
14	1.9843212166270244E-4	0.0	0.0	15.28509404359686	0.0
15	1.9843212166270244E-4	0.0	0.0	15.7246873371203	0.0
16	1.9843212166270244E-4	0.0	0.0	16.481705881263508	0.0
17	1.9843212166270244E-4	0.0	0.0	17.515008082801756	0.0
18	1.9843212166270244E-4	0.0	0.0	18.582242177144323	0.0
19	1.9843212166270244E-4	0.0	0.0	19.48854782081844	0.0
20	2.645761622169366E-4	0.0	0.0	20.322227307964006	0.0
21	2.645761622169366E-4	0.0	0.0	21.52346922846945	0.0
22	2.645761622169366E-4	0.0	0.0	22.817577381913043	0.0
23	2.645761622169366E-4	0.0	0.0	24.10321909816569	0.0
24	2.645761622169366E-4	0.0	0.0	25.039091127967552	0.0
25	2.645761622169366E-4	0.0	0.0	25.8748210803703	0.0
26	2.645761622169366E-4	0.0	0.0	26.59215320018097	0.0
27	2.645761622169366E-4	0.0	0.0	27.370800845585414	0.0
28	2.645761622169366E-4	0.0	0.0	28.152491116855355	0.0
29	2.645761622169366E-4	0.0	0.0	28.964806078901905	0.0
30	2.645761622169366E-4	0.0	0.0	29.89174866322894	0.0
31	2.645761622169366E-4	0.0	0.0	30.69235613009739	0.0
32	2.645761622169366E-4	0.0	0.0	31.464720091649184	0.0
33	2.645761622169366E-4	0.0	0.0	32.23060193722666	0.0
34	3.3072020277117074E-4	0.0	0.0	32.99760823149356	0.0
35	3.968642433254049E-4	0.0	0.0	33.85543029344142	0.0
36	3.968642433254049E-4	0.0	0.0	34.60662816201586	0.0
37	3.968642433254049E-4	0.0	0.0	35.373899032444974	0.0
38	3.968642433254049E-4	0.0	0.0	36.182311496098826	0.0
39	3.968642433254049E-4	0.0	0.0	37.37184592142617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGA	20	7.0339936E-4	45.0	40
CGTTTAT	1415	0.0	43.091873	1
TCGTTAG	200	0.0	42.75	1
CGTTTTT	3860	0.0	42.493523	1
CGTTAGG	445	0.0	42.471912	2
CCGTTAG	70	0.0	41.785717	1
TTAGGGA	2800	0.0	41.625	4
GTAGGGA	765	0.0	41.47059	4
TTTAGGG	5080	0.0	41.32382	3
GCGTTAG	170	0.0	41.02941	1
CGTAGGG	620	0.0	40.645164	3
TAGGGAA	1225	0.0	40.59184	5
TCTCGCG	100	0.0	40.5	1
CTTAGGG	2760	0.0	40.43478	3
TCTCGAG	145	0.0	40.344826	1
TTAGGGG	5010	0.0	40.32934	4
TAGGGTA	620	0.0	40.28226	5
TAGGGAT	1565	0.0	40.255592	5
AGGGCGA	750	0.0	40.199997	6
GTTAGGG	2285	0.0	40.076584	3
>>END_MODULE