##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934281.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	552951
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.284551434033034	33.0	31.0	34.0	31.0	34.0
2	32.45436937450154	34.0	31.0	34.0	31.0	34.0
3	32.50048919343667	34.0	31.0	34.0	30.0	34.0
4	35.99813003322175	37.0	35.0	37.0	35.0	37.0
5	35.122623885299056	37.0	35.0	37.0	35.0	37.0
6	26.08653388817454	36.0	0.0	37.0	0.0	37.0
7	31.090968277478474	37.0	19.0	37.0	17.0	37.0
8	34.695687321299715	37.0	32.0	37.0	32.0	37.0
9	37.208481402511254	39.0	37.0	39.0	34.0	39.0
10	37.34332698557377	39.0	37.0	39.0	34.0	39.0
11	37.392848552584226	39.0	37.0	39.0	35.0	39.0
12	37.03714614857374	39.0	35.0	39.0	34.0	39.0
13	36.97518767485727	39.0	37.0	39.0	33.0	39.0
14	37.957026933670434	40.0	37.0	41.0	33.0	41.0
15	38.13202616506707	40.0	37.0	41.0	33.0	41.0
16	38.28864221242027	40.0	37.0	41.0	34.0	41.0
17	38.300451577083685	40.0	37.0	41.0	34.0	41.0
18	38.28747393530349	40.0	37.0	41.0	34.0	41.0
19	38.29337500067818	40.0	37.0	41.0	34.0	41.0
20	38.19178552891667	40.0	36.0	41.0	34.0	41.0
21	38.1053673833667	40.0	36.0	41.0	34.0	41.0
22	38.12552468482741	40.0	36.0	41.0	34.0	41.0
23	38.07558535928138	40.0	36.0	41.0	34.0	41.0
24	38.013833052114926	40.0	36.0	41.0	34.0	41.0
25	37.90172366086688	40.0	36.0	41.0	34.0	41.0
26	37.84544019271147	40.0	36.0	41.0	34.0	41.0
27	37.82231698649609	40.0	35.0	41.0	34.0	41.0
28	37.77160905758377	40.0	36.0	41.0	34.0	41.0
29	37.78424670540428	40.0	36.0	41.0	34.0	41.0
30	37.570669010454814	40.0	36.0	41.0	33.0	41.0
31	37.56695439559744	40.0	35.0	41.0	33.0	41.0
32	37.47559548676103	40.0	35.0	41.0	33.0	41.0
33	37.39210888487407	40.0	35.0	41.0	33.0	41.0
34	37.32756247841129	40.0	35.0	41.0	33.0	41.0
35	37.28361283368689	40.0	35.0	41.0	33.0	41.0
36	37.1816634747021	39.0	35.0	41.0	33.0	41.0
37	37.10828084224461	39.0	35.0	41.0	32.0	41.0
38	37.053357349927936	39.0	35.0	41.0	32.0	41.0
39	37.02878374394838	39.0	35.0	41.0	32.0	41.0
40	36.94598798085183	39.0	35.0	41.0	32.0	41.0
41	36.84297342802527	39.0	35.0	41.0	32.0	41.0
42	36.811428137393726	39.0	35.0	41.0	32.0	41.0
43	36.730578297172805	39.0	35.0	41.0	31.0	41.0
44	36.62701577535803	39.0	35.0	41.0	31.0	41.0
45	36.608368553452294	39.0	35.0	41.0	31.0	41.0
46	36.57032720801662	39.0	35.0	41.0	31.0	41.0
47	36.48083103204443	39.0	35.0	40.0	31.0	41.0
48	36.47377977433805	39.0	35.0	40.0	31.0	41.0
49	36.47179587341374	39.0	35.0	40.0	31.0	41.0
50	36.374566643337296	39.0	35.0	40.0	31.0	41.0
51	35.40217125929784	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	8.0
12	6.0
13	3.0
14	7.0
15	11.0
16	24.0
17	43.0
18	78.0
19	167.0
20	270.0
21	537.0
22	864.0
23	1183.0
24	1829.0
25	2882.0
26	3846.0
27	4669.0
28	5333.0
29	6276.0
30	8012.0
31	10756.0
32	15359.0
33	25956.0
34	38719.0
35	49641.0
36	50708.0
37	82570.0
38	116899.0
39	126213.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.967673446652597	4.502207248020168	53.22153319191031	17.308586113416922
2	21.532830214612144	5.097558373165072	51.62808277767831	21.741528634544473
3	21.18940014576337	5.46124340131404	50.57880354678805	22.77055290613454
4	19.294838059791918	4.993209163198909	51.412331291561095	24.29962148544808
5	18.34918464746424	7.7968933956173325	49.43693021623978	24.41699174067865
6	15.924738358371718	31.265699854055782	39.41524655891752	13.39431522865498
7	80.79160721293569	2.2045353024047336	11.654558903049276	5.349298581610305
8	81.01477346093958	3.1254125591598534	9.953142321833218	5.906671658067351
9	75.59241234756787	5.096834981761495	12.278664836486415	7.032087834184223
10	41.560463766228835	24.650466316183532	19.078724877972913	14.710345039614722
11	31.51436564903581	23.53807118533107	25.89379529108366	19.05376787454946
12	29.64060106591723	20.449732435604602	30.860962363753753	19.048704134724414
13	25.676958717861076	24.069221323408403	31.205115824006107	19.048704134724414
14	19.668831415441876	26.9602550678089	32.267777795862564	21.103135720886662
15	16.598215755103073	24.68555079925708	38.772513296838234	19.94372014880161
16	20.570357952151276	22.766755101265755	34.12164911538274	22.541237831200235
17	20.365819032789524	23.614931521961257	29.6717068962711	26.34754254897812
18	20.977989008067624	22.959719758170255	33.40928943070905	22.653001803053073
19	23.025548375895873	25.207116001237	29.942797824762053	21.824537798105077
20	24.615381833109986	26.957542350045482	30.091093062495595	18.33598275434894
21	23.72723803736678	26.109727625051764	31.039640040437575	19.123394297143868
22	20.822640704149194	23.714397839953268	30.426565825905005	25.03639562999253
23	19.813509696157524	25.92833723060452	30.006275420426043	24.251877652811913
24	21.6107756383477	24.29618537628108	30.697475906545062	23.395563078826154
25	20.649569310843095	24.899674654716243	28.6634801275339	25.78727590690676
26	19.102054250738313	26.313181457308154	29.953106152263036	24.631658139690497
27	20.578134409739743	25.93032655696436	30.751911109664327	22.73962792363157
28	17.038218576329548	24.589158894730275	32.97670137136925	25.39592115757092
29	18.685019106575446	23.436796388830114	32.9018303610989	24.976354143495534
30	22.42856962009292	22.471611408605828	31.94821964333187	23.151599327969386
31	22.20232895862382	24.551542541744205	29.747120450094133	23.499008049537842
32	24.341035643302934	24.9076319601556	27.85924973460578	22.892082661935685
33	23.808077026716653	22.52803593808493	30.174825617459778	23.489061417738643
34	19.97301750064653	22.20504167638724	33.40314060377864	24.418800219187595
35	21.18433640593832	21.738815916781054	31.178531189924602	25.89831648735602
36	23.48942311344043	22.523876437514353	31.722702373266348	22.263998075778865
37	21.538255650138982	24.619541333680562	30.318599658920952	23.523603357259503
38	20.412296930469427	24.73962430667455	28.278274205128483	26.56980455772754
39	19.83503059041398	22.475409213474613	32.69041922340316	24.99914097270825
40	22.941996668782586	21.29718546489653	33.383609035882024	22.377208830438864
41	20.736918822825167	21.31237668437167	31.984027517808993	25.96667697499417
42	22.642331779850295	21.076912782506948	31.70895793659836	24.571797501044397
43	21.88566437170744	21.305685313888574	31.218679412823196	25.589970901580788
44	20.499103898898817	21.782400248846642	29.839533701901257	27.87896215035329
45	20.436530542489297	21.575329459572366	29.12862080003472	28.85951919790361
46	22.176648563796793	21.931780573685554	31.864848784069476	24.02672207844818
47	18.653370732668897	22.211190503317653	34.753893202110135	24.381545561903316
48	19.933954364853303	21.081433978779312	32.94469130176091	26.039920354606465
49	21.06063647592644	19.37676213624715	34.591130136305026	24.971471251521383
50	20.54467755732425	20.279373760061922	31.969197994035635	27.20675068857819
51	18.832771800756305	20.512848335566805	29.6948554211856	30.959524442491286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	305.0
2	529.0
3	2484.0
4	4439.0
5	2849.5
6	1260.0
7	1319.5
8	1379.0
9	1418.0
10	1457.0
11	1487.5
12	1518.0
13	1547.0
14	1576.0
15	1554.5
16	1533.0
17	1415.5
18	1298.0
19	1224.5
20	1151.0
21	1258.0
22	1365.0
23	1757.0
24	2149.0
25	2431.0
26	3242.5
27	3772.0
28	4723.0
29	5674.0
30	6922.0
31	8170.0
32	9160.0
33	10150.0
34	11277.5
35	12405.0
36	13275.5
37	14146.0
38	16300.0
39	18454.0
40	21702.0
41	24950.0
42	28689.0
43	32428.0
44	37623.5
45	42819.0
46	49996.0
47	57173.0
48	63527.0
49	69881.0
50	65880.5
51	61880.0
52	52427.0
53	42974.0
54	36995.0
55	31016.0
56	28114.5
57	25213.0
58	23204.5
59	21196.0
60	18836.0
61	16476.0
62	14076.5
63	11677.0
64	9617.0
65	7557.0
66	6207.5
67	4858.0
68	3967.5
69	3077.0
70	2491.0
71	1905.0
72	1493.5
73	1082.0
74	950.0
75	654.0
76	490.0
77	322.0
78	154.0
79	103.0
80	52.0
81	30.5
82	9.0
83	6.5
84	4.0
85	9.5
86	15.0
87	18.5
88	22.0
89	13.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	552951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.79585233944664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25717302962727	24.446582185258634
2	12.10746822218186	8.42579352927165
3	4.589358648276706	4.79071937574596
4	2.427840555360356	3.3791512587215626
5	1.5433854158467264	2.6851705516329067
6	1.0668500478430207	2.22731740398464
7	0.8796262763775214	2.1425142218710658
8	0.725116946679983	2.018484976440567
9	0.6212045613387273	1.9453807970039767
>10	5.5907990631523035	38.40410184714533
>50	0.1354841457389963	3.198447622345993
>100	0.04712492025703687	2.7128574968675356
>500	0.005355104574663281	1.3591306121403808
>1k	0.0032130627447979686	2.2643481215697707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	2569	0.4645981289481346	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	2561	0.46315134614097814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2041	0.3691104636758049	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	1956	0.353738396349767	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	1942	0.3512065264372431	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGC	1083	0.19585822251881269	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCG	992	0.17940106808740738	No Hit
CTGTCACTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT	793	0.14341234575938916	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	758	0.13708267097807944	No Hit
GCTGTATCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	748	0.1352741924691338	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTC	743	0.13436995321466097	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	741	0.13400825751287185	No Hit
CCTGTATCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	721	0.13039130049498057	No Hit
TCTGTATCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC	660	0.11935958159041218	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTG	620	0.11212566755462962	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8084785089456389E-4	0.0	0.0	0.49154445873142466	0.0
2	1.8084785089456389E-4	0.0	0.0	1.7656175682836273	0.0
3	1.8084785089456389E-4	0.0	0.0	2.482860144931468	0.0
4	1.8084785089456389E-4	0.0	0.0	3.257431490312885	0.0
5	1.8084785089456389E-4	0.0	0.0	4.5204728809605195	0.0
6	1.8084785089456389E-4	0.0	0.0	5.249289720065612	0.0
7	1.8084785089456389E-4	0.0	0.0	6.211581134675586	0.0
8	1.8084785089456389E-4	0.0	0.0	7.632864394855964	0.0
9	1.8084785089456389E-4	0.0	0.0	8.264204242328887	0.0
10	1.8084785089456389E-4	0.0	0.0	9.666136782463546	0.0
11	3.6169570178912777E-4	0.0	0.0	12.101253094758848	0.0
12	3.6169570178912777E-4	0.0	0.0	14.410137607129746	0.0
13	3.6169570178912777E-4	0.0	0.0	15.13389070640979	0.0
14	3.6169570178912777E-4	0.0	0.0	15.399917895075694	0.0
15	3.6169570178912777E-4	0.0	0.0	15.779336686252488	0.0
16	3.6169570178912777E-4	0.0	0.0	16.679778135856523	0.0
17	3.6169570178912777E-4	0.0	0.0	17.878618539436587	0.0
18	3.6169570178912777E-4	0.0	0.0	19.23081792057524	0.0
19	3.6169570178912777E-4	0.0	0.0	20.097259974211095	0.0
20	3.6169570178912777E-4	0.0	0.0	20.99137174903382	0.0
21	3.6169570178912777E-4	0.0	0.0	22.214988308186438	0.0
22	3.6169570178912777E-4	0.0	0.0	23.485625308571645	0.0
23	3.6169570178912777E-4	0.0	0.0	24.724433087199408	0.0
24	5.425435526836917E-4	0.0	0.0	25.564290506753764	0.0
25	5.425435526836917E-4	0.0	0.0	26.348446788232593	0.0
26	5.425435526836917E-4	0.0	0.0	27.001126682111074	0.0
27	5.425435526836917E-4	0.0	0.0	27.721986215776806	0.0
28	5.425435526836917E-4	0.0	0.0	28.437601161766594	0.0
29	5.425435526836917E-4	0.0	0.0	29.2291722051321	0.0
30	5.425435526836917E-4	0.0	0.0	30.078614560783866	0.0
31	5.425435526836917E-4	0.0	0.0	30.87561103967621	0.0
32	5.425435526836917E-4	0.0	0.0	31.59411955128031	0.0
33	5.425435526836917E-4	0.0	0.0	32.312808910735306	0.0
34	5.425435526836917E-4	0.0	0.0	33.069114623176375	0.0
35	5.425435526836917E-4	0.0	0.0	33.83048407544249	0.0
36	5.425435526836917E-4	0.0	0.0	34.6084915299909	0.0
37	5.425435526836917E-4	0.0	0.0	35.3107237350145	0.0
38	5.425435526836917E-4	0.0	0.0	36.09524171219511	0.0
39	5.425435526836917E-4	0.0	0.0	37.19154138431796	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	65	0.0	45.000004	1
CGTTCAT	50	2.1827873E-11	45.000004	17
CGGTGTA	25	3.88817E-5	45.000004	13
TAGTCCG	25	3.88817E-5	45.000004	1
CTCACGC	20	7.030107E-4	45.0	19
GTTTACG	20	7.030107E-4	45.0	1
CCGTCCC	20	7.030107E-4	45.0	13
TATTGCG	20	7.030107E-4	45.0	1
CGAATAT	35	1.2103919E-7	45.0	14
GTCCATT	20	7.030107E-4	45.0	22
ACTCACG	20	7.030107E-4	45.0	18
TCGACGT	45	3.8380676E-10	45.0	26
CGTTTAT	335	0.0	45.0	1
TAGCTAT	20	7.030107E-4	45.0	23
TTCGACG	45	3.8380676E-10	45.0	25
CCGCACA	20	7.030107E-4	45.0	13
TCGCGAG	20	7.030107E-4	45.0	1
CGGGTAC	30	2.1634514E-6	44.999996	6
TAGGTTC	30	2.1634514E-6	44.999996	5
TACGCAC	30	2.1634514E-6	44.999996	19
>>END_MODULE